FastQCFastQC Report
Fri 27 May 2016
SRR940149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844708
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75150.8896565440365191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61000.7221430364102152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40490.4793372384303215No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.18621819611037185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.15508317667170193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12650.1497558919768725No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.14857205093357703No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10740.12714452804992968No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT9910.11731864739057757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.090.783291
GTATCAA53250.065.656761
ATCAACG72400.048.3611683
TCAACGC73250.047.8812754
CAACGCA74050.047.3303385
TATCAAC74050.047.2059562
AACGCAG74850.046.8244676
ACGCAGA85900.040.793827
TGGTATC6750.040.582412
CGCAGAG87200.040.1856588
GTGGTAT7500.038.118951
GCAGAGT95950.035.9629449
TATCTCG350.002788335334.00253726-27
GTACATG60550.031.969081
GAGTACT59750.031.01647212-13
ACATGGG64250.029.5648423
CAGAGTA94000.029.3671910-11
TACATGG65450.029.2974592
AGAGTAC92250.028.76343310-11
AGTACTT63000.028.51928712-13
Produced by FastQC (version 0.11.5)