##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940149_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 844708 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.715949180071696 34.0 31.0 34.0 31.0 34.0 2 32.78403069463057 34.0 31.0 34.0 31.0 34.0 3 32.88206812294899 34.0 31.0 34.0 31.0 34.0 4 36.297719448614195 37.0 37.0 37.0 35.0 37.0 5 36.26498269224395 37.0 37.0 37.0 35.0 37.0 6 36.322229693574585 37.0 37.0 37.0 35.0 37.0 7 36.30359485171207 37.0 37.0 37.0 35.0 37.0 8 36.287050673132015 37.0 37.0 37.0 35.0 37.0 9 38.097340145943924 39.0 39.0 39.0 35.0 39.0 10-11 38.07408299672787 39.0 39.0 39.0 35.0 39.0 12-13 38.07130570564029 39.0 39.0 39.0 35.0 39.0 14-15 39.53597811314679 41.0 39.5 41.0 36.5 41.0 16-17 39.526283638843246 41.0 39.0 41.0 36.5 41.0 18-19 39.59492984557978 41.0 40.0 41.0 36.5 41.0 20-21 39.580940395971155 41.0 40.0 41.0 37.0 41.0 22-23 39.5312042741397 41.0 40.0 41.0 36.0 41.0 24-25 39.49601519104827 41.0 39.5 41.0 36.0 41.0 26-27 39.36912518882265 41.0 39.0 41.0 35.5 41.0 28-29 39.24274956553034 41.0 39.0 41.0 35.0 41.0 30-31 39.02017087561619 41.0 38.5 41.0 35.0 41.0 32-33 38.76215272022995 41.0 38.0 41.0 35.0 41.0 34-35 38.59842572818063 40.5 38.0 41.0 35.0 41.0 36-37 38.44938546811443 40.0 38.0 41.0 35.0 41.0 38-39 38.27336191914839 40.0 38.0 41.0 34.0 41.0 40-41 38.13439141099646 40.0 38.0 41.0 34.0 41.0 42-43 37.92111238439792 40.0 37.5 41.0 33.5 41.0 44-45 37.885926260909095 40.0 38.0 41.0 33.5 41.0 46-47 37.51321048220213 40.0 37.5 41.0 33.0 41.0 48-49 37.133452033128606 40.0 37.0 41.0 31.5 41.0 50-51 36.916365773734825 40.0 37.0 41.0 31.0 41.0 52-53 36.782257300747716 40.0 36.5 41.0 31.0 41.0 54-55 36.594644539888336 40.0 36.0 41.0 31.0 41.0 56-57 36.421904374055885 40.0 35.5 41.0 31.0 41.0 58-59 36.19646789186322 39.5 35.0 41.0 30.0 41.0 60-61 35.95090907153715 39.0 35.0 41.0 30.0 41.0 62-63 35.69272517840484 39.0 35.0 41.0 29.5 41.0 64-65 35.39915331688584 38.5 35.0 41.0 29.0 41.0 66-67 35.097578689914144 37.0 35.0 40.5 29.0 41.0 68-69 34.75564988137913 37.0 35.0 40.0 29.0 41.0 70-71 34.490557683838674 36.0 35.0 39.0 28.5 41.0 72-73 34.29959287706521 36.0 35.0 39.0 29.0 41.0 74-75 33.98949341074076 35.5 35.0 39.0 29.0 41.0 76-77 33.65787053040815 35.0 35.0 37.0 29.0 39.5 78-79 33.332643943232455 35.0 35.0 37.0 28.5 39.0 80-81 33.04950764051009 35.0 34.0 36.5 28.0 39.0 82-83 32.782556812531666 35.0 34.0 36.0 27.5 37.5 84-85 32.55117212101697 35.0 34.0 36.0 27.0 37.0 86-87 32.349283421016494 35.0 34.0 35.5 27.0 37.0 88-89 32.17529844632702 35.0 34.0 35.0 27.0 36.0 90-91 32.05123959995643 35.0 34.0 35.0 26.5 36.0 92-93 31.91215899458748 35.0 34.0 35.0 26.0 36.0 94-95 31.806814307429313 35.0 34.0 35.0 25.0 36.0 96-97 31.723594425529296 35.0 34.0 35.0 25.0 35.5 98-99 31.655259568987155 35.0 34.0 35.0 25.0 35.0 100-101 31.61284017672379 35.0 34.0 35.0 25.0 35.0 102-103 30.849827395975886 34.5 32.0 35.0 23.5 35.0 104-105 31.436062521013177 35.0 33.5 35.0 24.0 35.0 106-107 31.481878945150278 35.0 34.0 35.0 24.0 35.0 108-109 31.47314456593284 35.0 34.0 35.0 24.0 35.0 110-111 31.429560274082878 35.0 34.0 35.0 24.0 35.0 112-113 31.37014329211988 35.0 34.0 35.0 24.0 35.0 114-115 31.289656899188834 35.0 34.0 35.0 23.0 35.0 116-117 31.20580662193326 35.0 34.0 35.0 20.0 35.0 118-119 31.115529271653635 35.0 34.0 35.0 19.0 35.0 120-121 31.00463888112815 35.0 34.0 35.0 17.5 35.0 122-123 30.843348825866453 35.0 34.0 35.0 2.0 35.0 124-125 29.174994199178887 33.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1355815991542002 1101 2 0.07717510829312602 1101 3 0.05185907023912506 1101 4 0.05265682952684614 1101 5 0.028081396274991732 1101 6 0.12797864965290273 1101 7 0.2050831609226833 1101 8 0.18815333676597135 1101 9 0.1731689047459426 1101 10-11 0.20963696902949636 1101 12-13 0.16940725515571842 1101 14-15 0.2594138144427731 1101 16-17 0.22500310293458625 1101 18-19 0.20312549184366446 1101 20-21 0.2779901791365802 1101 22-23 0.285748794401556 1101 24-25 0.2588909426262589 1101 26-27 0.2955578766569218 1101 28-29 0.20128387441704376 1101 30-31 0.23169364625726985 1101 32-33 0.30804619114510956 1101 34-35 0.4017201972887534 1101 36-37 0.44734821699329785 1101 38-39 0.4068619429699041 1101 40-41 0.44493940420276346 1101 42-43 0.30071813335428743 1101 44-45 0.29258166680598663 1101 46-47 0.15373833065408604 1101 48-49 0.3780009175403336 1101 50-51 0.48395747235753817 1101 52-53 0.3746279110748034 1101 54-55 0.31243176897281444 1101 56-57 0.33667776663491367 1101 58-59 0.28281646368739644 1101 60-61 0.28233596933377214 1101 62-63 0.2723269631862806 1101 64-65 0.21723024594524531 1101 66-67 0.11052582575680958 1101 68-69 0.028468002093894995 1101 70-71 0.25098820514386233 1101 72-73 0.1439758828463198 1101 74-75 0.13853045408690434 1101 76-77 0.14786809342428597 1101 78-79 0.08683953192880267 1101 80-81 0.07198507513728458 1101 82-83 0.06991293779102392 1101 84-85 0.034830599118805594 1101 86-87 0.07310223945820837 1101 88-89 0.08075606921870104 1101 90-91 0.0783451604049148 1101 92-93 0.11095446321679603 1101 94-95 0.09875584025727946 1101 96-97 0.10192595694952189 1101 98-99 0.10881183124840987 1101 100-101 0.010713643281501817 1101 102-103 0.08247425374378281 1101 104-105 0.010754071881578398 1101 106-107 0.07428926602953823 1101 108-109 0.17332613377693562 1101 110-111 0.005287960385029322 1101 112-113 -0.021420663089486425 1101 114-115 0.12778293864042567 1101 116-117 0.08859623475746403 1101 118-119 0.11059221884763915 1101 120-121 0.05673992315400156 1101 122-123 0.02281989709479504 1101 124-125 0.03176467151325113 1102 1 0.09419052637431236 1102 2 0.13261208225780763 1102 3 0.09309272956753034 1102 4 0.05077075642923745 1102 5 -0.029217100065508816 1102 6 0.09073376053551385 1102 7 0.15504590159196852 1102 8 0.15966583452752303 1102 9 0.1740729489719044 1102 10-11 0.15683053269673763 1102 12-13 0.18791561106608867 1102 14-15 0.2800941871516116 1102 16-17 0.20656406670927652 1102 18-19 0.2448487214477879 1102 20-21 0.30898546539799554 1102 22-23 0.2585928077802535 1102 24-25 0.3011217945074165 1102 26-27 0.26204614141190774 1102 28-29 0.2245955068378649 1102 30-31 0.22301424402402859 1102 32-33 0.1762622490501542 1102 34-35 0.11975948999690189 1102 36-37 0.31778972562071317 1102 38-39 0.34614656382011333 1102 40-41 0.35493718019620957 1102 42-43 0.2741513640559745 1102 44-45 0.22470180937347095 1102 46-47 0.14097339948275334 1102 48-49 0.10977561701061944 1102 50-51 0.030177094449719277 1102 52-53 0.08416543324266001 1102 54-55 0.16546609377019195 1102 56-57 0.13623195441038405 1102 58-59 0.07361254250388072 1102 60-61 0.0971036617392258 1102 62-63 0.023321267418822345 1102 64-65 0.09570706139891882 1102 66-67 -0.06459158445975532 1102 68-69 0.012808251991351938 1102 70-71 0.04638321181587912 1102 72-73 -0.06377232495098895 1102 74-75 -0.011737293196631526 1102 76-77 -0.01002048673525735 1102 78-79 0.10059660109251922 1102 80-81 -0.013948227772409894 1102 82-83 -0.07935400653949642 1102 84-85 -0.017130281472262254 1102 86-87 -0.1398897136816828 1102 88-89 -0.23489689333133867 1102 90-91 0.025603711276900754 1102 92-93 0.04152285575106518 1102 94-95 -8.435497294883021E-4 1102 96-97 0.02547645345176619 1102 98-99 -0.03687576226095857 1102 100-101 -0.05939022071327216 1102 102-103 -0.23609519013904645 1102 104-105 -0.13498912179184686 1102 106-107 -0.24187853959806205 1102 108-109 -0.17255526601856985 1102 110-111 -0.15277897354753378 1102 112-113 -0.09492480205234699 1102 114-115 -0.13905669026819467 1102 116-117 -0.32112137417553654 1102 118-119 -0.20375666712725504 1102 120-121 -0.30688587185613514 1102 122-123 -0.31923014594117305 1102 124-125 -0.407270109638727 1103 1 0.027878588037367535 1103 2 0.07330188441033414 1103 3 -0.03232409990001628 1103 4 -0.07321816127047498 1103 5 -0.0031694319661923487 1103 6 0.050637856013793225 1103 7 0.011102449739588849 1103 8 0.051042242015178374 1103 9 -0.02205748117764017 1103 10-11 -0.001041452526855835 1103 12-13 -0.05433565878082902 1103 14-15 0.033896623836874085 1103 16-17 0.15160473733052982 1103 18-19 0.08473043920433554 1103 20-21 0.0563694031666131 1103 22-23 0.10738760762396993 1103 24-25 0.11666720707446387 1103 26-27 0.0011648854178716306 1103 28-29 0.06763887699379723 1103 30-31 0.06374915682195592 1103 32-33 0.15065680735655462 1103 34-35 0.104243203988311 1103 36-37 0.03503797625118921 1103 38-39 0.19758122442466686 1103 40-41 0.18203908763057797 1103 42-43 0.13569862367306484 1103 44-45 0.16469754695028627 1103 46-47 0.22865108924938937 1103 48-49 0.22925027335274706 1103 50-51 0.17276296451685624 1103 52-53 0.2958537080268755 1103 54-55 0.14489919148624608 1103 56-57 0.0818338440846631 1103 58-59 0.12791474830787308 1103 60-61 0.24390116111761984 1103 62-63 0.12922934115874796 1103 64-65 0.010247252497556758 1103 66-67 0.10381817648934089 1103 68-69 0.10085002373859453 1103 70-71 0.06334445928506938 1103 72-73 0.11144772264606928 1103 74-75 0.19776258248269585 1103 76-77 0.142035066846951 1103 78-79 0.11998316735544279 1103 80-81 -0.04577609691602191 1103 82-83 0.015351353457667472 1103 84-85 0.06519060795337595 1103 86-87 0.17470056088941277 1103 88-89 0.16718174837647126 1103 90-91 0.18857817225796936 1103 92-93 -0.023035450646116118 1103 94-95 0.039595124656898406 1103 96-97 0.21975486072630446 1103 98-99 0.22487786967880297 1103 100-101 0.16230018528253254 1103 102-103 0.01812212240313471 1103 104-105 -0.13547503803523142 1103 106-107 0.023709497200854912 1103 108-109 0.041787077603778755 1103 110-111 0.005958283583147761 1103 112-113 -0.034796574154785986 1103 114-115 0.044889247997712545 1103 116-117 0.11054168127148856 1103 118-119 0.0882576830308075 1103 120-121 -0.2491030423175431 1103 122-123 -0.08298036794238328 1103 124-125 -0.32502985105172044 1104 1 -0.0776744276123651 1104 2 -0.12864290194450945 1104 3 0.05440704129920704 1104 4 -0.12388811027697955 1104 5 -0.22998145939717318 1104 6 -0.020141113565948388 1104 7 -0.1364164530210914 1104 8 0.03453441715145544 1104 9 0.016704607797215942 1104 10-11 0.1685083980094575 1104 12-13 0.16740691199463242 1104 14-15 0.13048533493235226 1104 16-17 0.17508460369240453 1104 18-19 0.2127311425628733 1104 20-21 0.1634909083538858 1104 22-23 0.16162885960216045 1104 24-25 0.1494261309359004 1104 26-27 0.110749903881036 1104 28-29 0.224347159040569 1104 30-31 0.17253410494926413 1104 32-33 0.18249784832718774 1104 34-35 0.2839987868966958 1104 36-37 0.22829276788122854 1104 38-39 0.25478009905100407 1104 40-41 0.34052334998331446 1104 42-43 0.2276434100279161 1104 44-45 0.08415420248044114 1104 46-47 0.05266023291036248 1104 48-49 0.09399794347759638 1104 50-51 -0.023558470334556603 1104 52-53 0.03823281827832403 1104 54-55 -0.05546479655666303 1104 56-57 -0.09504676080367602 1104 58-59 0.09484282989746617 1104 60-61 -0.07854652659118955 1104 62-63 -0.08626564741167897 1104 64-65 -0.1440168586330941 1104 66-67 -0.20713839112099208 1104 68-69 -0.17635412607678091 1104 70-71 -0.20899420785050182 1104 72-73 -0.20206203645363274 1104 74-75 -0.10417165349409174 1104 76-77 -0.11575111215779543 1104 78-79 -0.07410158734826666 1104 80-81 -0.20039188731517044 1104 82-83 -0.1649036140119975 1104 84-85 -0.13379303896063988 1104 86-87 -0.14047264115032476 1104 88-89 -0.10801255441938196 1104 90-91 -0.11246455677315481 1104 92-93 -0.20688633863627004 1104 94-95 -0.07041542572115844 1104 96-97 0.0692484425796529 1104 98-99 0.0650923940325896 1104 100-101 0.055240223967253144 1104 102-103 0.1168525602438244 1104 104-105 0.03762501471666013 1104 106-107 -0.014544415231004137 1104 108-109 -0.0420567768010045 1104 110-111 0.0018313273672134756 1104 112-113 0.06582589815047157 1104 114-115 0.15687799426111937 1104 116-117 0.0492721191121781 1104 118-119 0.15530181877900517 1104 120-121 0.17963091231583306 1104 122-123 0.14537201468466066 1104 124-125 0.2169272440775245 1105 1 -0.1703804390660153 1105 2 -0.19061265606816846 1105 3 -0.04291472407235375 1105 4 -0.10236621459439021 1105 5 -0.20241724692160545 1105 6 -5.601664105014947E-4 1105 7 -0.1605617155292336 1105 8 -0.21971612295347853 1105 9 -0.23836894935102748 1105 10-11 -0.04372883665327976 1105 12-13 -0.015764104088489717 1105 14-15 -0.14260200775137122 1105 16-17 -0.07542978195966299 1105 18-19 -0.012449749724773085 1105 20-21 0.0028071183604012617 1105 22-23 0.030184464691153323 1105 24-25 0.14314376750528623 1105 26-27 0.12743485981533098 1105 28-29 0.06303924778924852 1105 30-31 -0.07042052623622652 1105 32-33 0.10159527296968918 1105 34-35 -0.10215566596843217 1105 36-37 0.12051868605961147 1105 38-39 0.13062969268634106 1105 40-41 0.013644985043846702 1105 42-43 0.11803640066609233 1105 44-45 0.13473374364843949 1105 46-47 0.06680240910460356 1105 48-49 0.040817325528962556 1105 50-51 -0.012156255047024445 1105 52-53 0.05930033996825301 1105 54-55 0.022949866863662294 1105 56-57 0.04180382731058074 1105 58-59 -0.1365312656410964 1105 60-61 0.07004577134098611 1105 62-63 0.04339725852432963 1105 64-65 -0.03568724030724724 1105 66-67 0.0772568628140533 1105 68-69 0.041217287797913116 1105 70-71 0.079920781347397 1105 72-73 0.12424193117436744 1105 74-75 0.004692925909907331 1105 76-77 0.11743190625720956 1105 78-79 0.08778929430874172 1105 80-81 0.08884030636817641 1105 82-83 0.04200801453297487 1105 84-85 0.06819934813170647 1105 86-87 0.08760424235846642 1105 88-89 0.041051263693852036 1105 90-91 0.04144477418257253 1105 92-93 0.06223337605115731 1105 94-95 0.12161331129060571 1105 96-97 0.17106765316913908 1105 98-99 0.21230613841721535 1105 100-101 0.1964133661777403 1105 102-103 0.3459565004379179 1105 104-105 0.22939827352122677 1105 106-107 0.29173280434308424 1105 108-109 0.17691376604732056 1105 110-111 0.18640147242396665 1105 112-113 0.29816360947724263 1105 114-115 0.21952330820782961 1105 116-117 0.14227647424403145 1105 118-119 0.232980910716865 1105 120-121 0.24361877281443967 1105 122-123 0.21937153459138514 1105 124-125 0.418729308478607 1106 1 -0.10095776604721607 1106 2 -0.07705973015931988 1106 3 -0.012858203743817853 1106 4 -0.08679665702391048 1106 5 -0.07048753020627885 1106 6 0.01887531332926784 1106 7 0.11551362214130734 1106 8 -0.08038582908191927 1106 9 0.13634546077140186 1106 10-11 -0.10618636102874035 1106 12-13 -0.07644405559371137 1106 14-15 -0.08920993425786605 1106 16-17 -0.10280030601068546 1106 18-19 -0.14130756097078034 1106 20-21 0.05412791818281448 1106 22-23 -0.1458686746630491 1106 24-25 -0.09784115471521915 1106 26-27 -0.16011871496738195 1106 28-29 -0.2119688631340324 1106 30-31 -0.12190183696142753 1106 32-33 -0.04508437117422659 1106 34-35 0.016769252495443254 1106 36-37 -0.049182319694871524 1106 38-39 -0.16324357200296902 1106 40-41 -0.2780869958997627 1106 42-43 -0.12332247780666705 1106 44-45 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-0.45203403287402466 2216 70-71 -0.4764619223546589 2216 72-73 -0.28971345191842346 2216 74-75 -0.18496574926616205 2216 76-77 -0.30694287994147373 2216 78-79 -0.4146624681974984 2216 80-81 -0.4140352723642451 2216 82-83 -0.3063770184609709 2216 84-85 -0.414147126202991 2216 86-87 -0.40165229049577533 2216 88-89 -0.3749351992555212 2216 90-91 -0.4620708375568512 2216 92-93 -0.3426482139255782 2216 94-95 -0.3480823923148364 2216 96-97 -0.3355752679509081 2216 98-99 -0.3465225829007714 2216 100-101 -0.44376143516656796 2216 102-103 -0.5520113751799833 2216 104-105 -0.48791160325087546 2216 106-107 -0.37788926021381286 2216 108-109 -0.4868965585650926 2216 110-111 -0.38800660802538545 2216 112-113 -0.5559451621868661 2216 114-115 -0.5678295769022021 2216 116-117 -0.557414346204645 2216 118-119 -0.4696387592652975 2216 120-121 -0.48732137848508117 2216 122-123 -0.6701359455169325 2216 124-125 -0.8816245245383207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 29.0 5 70.0 6 152.0 7 374.0 8 668.0 9 1205.0 10 2294.0 11 3038.0 12 3529.0 13 5351.0 14 9490.0 15 10324.0 16 3904.0 17 2993.0 18 2894.0 19 2720.0 20 2579.0 21 2523.0 22 2515.0 23 2606.0 24 3040.0 25 3743.0 26 4707.0 27 5792.0 28 7143.0 29 8697.0 30 10972.0 31 13489.0 32 17260.0 33 22671.0 34 32962.0 35 58751.0 36 117521.0 37 296726.0 38 180944.0 39 1023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.778563638753404 19.43129084473937 14.4320546021416 21.358090914365626 2 15.567323242009456 21.812129004150655 40.029075893290525 22.591471860549365 3 17.37491047064325 28.746470619572744 30.806859280568727 23.07175962921528 4 11.6530070346019 18.124793387450516 38.57691792727475 31.64528165067283 5 11.782136489663712 39.80139553495112 33.99609799525035 14.420369980134817 6 28.64283109218622 38.93450387681348 18.08354686989348 14.339118161106823 7 26.487022734483396 35.07969617903465 21.981915644222617 16.451365442259338 8 26.873428451015023 33.830743878357964 20.509454154571756 18.786373516055253 9 28.50073634912893 14.94918954242176 20.21574319173016 36.334330916719146 10-11 24.193863441567974 27.501160164222426 27.46854534347964 20.836431050729956 12-13 26.271149320238475 25.481231384099594 28.117882155727187 20.129737139934747 14-15 23.123848714585396 25.459093556589966 27.567987991116453 23.849069737708177 16-17 20.812339885498897 29.270706563688282 27.738520293403162 22.17843325740966 18-19 20.780080217069095 26.40385790119189 32.04758330689422 20.7684785748448 20-21 22.144752979727905 25.40599828579817 32.220779251528334 20.228469482945584 22-23 21.858085871093916 25.463177808189343 32.35112015039517 20.327616170321576 24-25 21.361341026037163 25.753806248753254 32.33676237559881 20.548090349610778 26-27 20.861587672899983 26.1881028710513 32.05948090937933 20.890828546669383 28-29 20.847731919341875 25.693292656218 32.50925475404907 20.949720670391063 30-31 20.993082202566086 25.567376600251237 32.567043911379265 20.87249728580342 32-33 20.757172892881325 25.95985831790394 32.176503596509086 21.106465192705645 34-35 21.247519853014296 25.853608584268173 32.032193373331374 20.866678189386153 36-37 21.53615759347938 25.436098993139616 31.494131087184286 21.533612326196717 38-39 21.203730285335457 25.731155459138222 31.69292329001459 21.37219096551173 40-41 21.311675304780596 25.546639896011158 31.896600710779843 21.2450840884284 42-43 21.30079269996259 25.672658480800465 31.312358826955588 21.71418999228136 44-45 21.26353126004467 25.77348757997998 31.18682032925499 21.77616083072036 46-47 21.81496595740147 26.014263440344653 30.353200098972742 21.817570503281136 48-49 21.828608229115858 26.512593701018577 29.511322255738076 22.147475814127485 50-51 22.131656506140416 26.39878156555567 29.177491637469583 22.292070290834335 52-53 22.229279230219202 26.374143491005174 29.310187662482186 22.08638961629344 54-55 21.804881571517022 26.674377609694478 29.262140887689757 22.25859993109874 56-57 21.98987930098632 26.67613348810146 29.024824657616954 22.309162553295263 58-59 22.053360231284497 26.127772371352737 29.245484056570938 22.573383340791832 60-61 22.042179197365236 26.503260603843586 29.311576638248475 22.1429835605427 62-63 21.91187090816532 26.55332851882984 29.7895627878343 21.74523778517054 64-65 21.702391388892668 26.28272480647917 29.742333307485175 22.27255049714299 66-67 21.881873693332356 26.67277135476722 29.236562780423007 22.208792171477413 68-69 21.83397557698341 26.547511231864757 29.551968461989237 22.066544729162597 70-71 21.85525293118645 26.266816548306345 29.573743009815118 22.30418751069209 72-73 21.786583810194116 26.78011089491975 28.860204564248786 22.573100730637343 74-75 21.681989515903723 27.09243904402468 28.992977454931175 22.23259398514043 76-77 21.80160011743981 26.579162957210233 29.323075502434037 22.296161422915915 78-79 21.72626499577067 26.571614775247674 29.191431478643516 22.51068875033814 80-81 21.730037977800507 27.099847994090236 28.7414289502595 22.428685077849757 82-83 21.934505470307975 26.639272211971992 28.95454166082137 22.47168065689866 84-85 21.707974826804055 26.564682706923577 28.904485336944834 22.82285712932753 86-87 21.855989145999217 26.68399502524964 28.819988504325085 22.64002732442606 88-89 21.83995614782823 26.69092506298422 28.743121460097363 22.725997329090188 90-91 21.739012042829472 26.523408456973996 28.9070528454322 22.83052665476433 92-93 21.908573586701653 26.50671020361773 28.617297295377416 22.967418914303202 94-95 21.61514692302228 26.222918097222554 28.759974427580325 23.401960552174838 96-97 21.884497040859696 26.267801309622325 28.760902173745755 23.086799475772224 98-99 21.571983072208315 26.46758140831697 28.566625209481128 23.39381030999359 100-101 21.368483675335863 26.312085075903546 28.906391051994447 23.413040196766143 102-103 21.806209644767407 26.0430503169225 28.62969198021513 23.52104805809496 104-105 21.425142434332223 26.178734739178687 28.80443951165372 23.591683314835368 106-107 21.6239477120469 25.9146662688956 28.809220238496895 23.652165780560598 108-109 21.698670002267274 26.065516610616186 28.246838391689572 23.988974995426968 110-111 21.647614131708735 26.121191170168185 28.417143966605185 23.814050731517895 112-113 21.270473438176122 26.30799438591673 28.81509338356253 23.606438792344626 114-115 21.446345956235767 26.331821173707365 28.350625304839067 23.8712075652178 116-117 21.117960950980073 26.17799652542448 28.593862372478046 24.1101801511174 118-119 20.873853358991497 26.399809161999798 28.339206258349904 24.387131220658805 120-121 20.85246227681584 26.58469337260538 28.474226535623043 24.088617814955736 122-123 20.78672835413786 26.61580370503665 28.38379377947834 24.21367416134715 124-125 20.70233159118337 26.616818201736464 28.16900157397505 24.511848633105114 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1301.0 1 837.0 2 858.0 3 1181.0 4 601.5 5 304.5 6 791.5 7 2103.0 8 3728.5 9 4108.0 10 2671.0 11 1227.0 12 906.0 13 931.5 14 1028.0 15 1136.0 16 1251.0 17 1390.0 18 1550.5 19 1651.5 20 1784.5 21 2012.5 22 2327.0 23 2667.5 24 3053.5 25 3681.0 26 4443.5 27 5502.5 28 6639.0 29 8053.0 30 10298.5 31 12794.0 32 15934.0 33 18302.0 34 19706.0 35 20914.0 36 23519.5 37 26445.5 38 26505.0 39 26214.5 40 25964.5 41 26118.5 42 25917.5 43 26782.0 44 29225.0 45 30337.0 46 29118.0 47 28026.5 48 27693.5 49 26707.0 50 26290.5 51 25032.0 52 23802.5 53 24256.0 54 25029.0 55 24063.5 56 22184.5 57 20679.0 58 19796.5 59 18092.5 60 14719.0 61 11947.0 62 10410.5 63 8214.5 64 6262.5 65 5208.5 66 3848.0 67 2846.5 68 1962.5 69 1177.5 70 818.5 71 589.0 72 481.5 73 432.0 74 324.5 75 240.5 76 180.5 77 109.5 78 55.0 79 25.5 80 15.5 81 8.0 82 5.5 83 4.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02355843676157915 2 0.002604450295249956 3 0.001538993356284065 4 0.08724908489087353 5 0.0016573774606136085 6 0.023676820865908693 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013614171997897498 26-27 0.0 28-29 8.286887303068042E-4 30-31 0.00887880782471576 32-33 0.0 34-35 0.0 36-37 3.551523129886304E-4 38-39 5.9192052164771734E-5 40-41 2.3676820865908694E-4 42-43 0.0 44-45 0.005149708538335141 46-47 0.0036699072342158477 48-49 0.0 50-51 0.0020717218257670107 52-53 0.0 54-55 0.0024860661909204126 56-57 4.143443651534021E-4 58-59 0.004025059547204478 60-61 2.9596026082385866E-4 62-63 0.004498595964522651 64-65 0.003729099286380619 66-67 2.3676820865908694E-4 68-69 0.001538993356284065 70-71 0.004912940329676054 72-73 0.004084251599369249 74-75 0.0 76-77 0.0023676820865908693 78-79 0.0013614171997897498 80-81 4.735364173181739E-4 82-83 0.0013614171997897498 84-85 0.0 86-87 0.005031324434005597 88-89 0.00615597342513626 90-91 0.006629509842454434 92-93 0.0072806224162669225 94-95 0.005682437007818087 96-97 0.004380211860193109 98-99 0.0072806224162669225 100-101 0.0059783972686419445 102-103 0.002604450295249956 104-105 0.002426874138755641 106-107 1.1838410432954347E-4 108-109 0.009766688607187336 110-111 0.00307798671256813 112-113 0.0055048608513237705 114-115 0.0 116-117 5.9192052164771734E-5 118-119 0.0014798013041192933 120-121 2.3676820865908694E-4 122-123 0.0023676820865908693 124-125 0.04131605241101067 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 844708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.05076002217927 #Duplication Level Percentage of deduplicated Percentage of total 1 65.98225264044298 25.106748609443002 2 15.162662305396546 11.53901649359975 3 6.50539105306953 7.426052214323422 4 3.5367503029229024 5.383041481395568 5 2.116160984082756 4.0260766886815835 6 1.3796111197761132 3.1497150985518543 7 0.978975010695495 2.607552023977926 8 0.6848168732400024 2.0846242002235593 9 0.5222635793869079 1.7885273514818043 >10 2.785934502058235 19.36338357607089 >50 0.2265936933868757 5.9269546441656935 >100 0.11329661033378012 8.067414684194112 >500 0.0028012898159023364 0.6717614705339506 >1k 0.001867526543934891 1.24664337779273 >5k 6.22508847978297E-4 1.6124880855641288 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7515 0.8896565440365191 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6100 0.7221430364102152 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4049 0.4793372384303215 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1573 0.18621819611037185 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1310 0.15508317667170193 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1265 0.1497558919768725 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.14857205093357703 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1074 0.12714452804992968 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 991 0.11731864739057757 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.9192052164771734E-5 0.0 18-19 0.0 0.0 0.0 1.775761564943152E-4 0.0 20-21 0.0 0.0 0.0 3.551523129886304E-4 0.0 22-23 0.0 0.0 0.0 0.0011838410432954347 0.0 24-25 0.0 0.0 0.0 0.0025452582430851847 0.0 26-27 0.0 0.0 0.0 0.003906675442874935 0.0 28-29 0.0 0.0 0.0 0.007043854207607836 0.0 30-31 0.0 0.0 0.0 0.015508317667170195 0.0 32-33 0.0 0.0 0.0 0.02876733735207906 0.0 34-35 0.0 0.0 0.0 0.04036897957637432 0.0 36-37 0.0 0.0 0.0 0.060908621677550116 0.0 38-39 0.0 0.0 0.0 0.09683819734156655 0.0 40-41 0.0 0.0 0.0 0.14478375959503165 0.0 42-43 0.0 0.0 0.0 0.1876388053623264 0.0 44-45 0.0 0.0 0.0 0.2338677981030131 0.0 46-47 0.0 0.0 0.0 0.2861935722166713 0.0 48-49 0.0 0.0 0.0 0.3484044190418464 0.0 50-51 0.0 0.0 0.0 0.42760338483831095 0.0 52-53 0.0 0.0 0.0 0.5016526420964404 0.0 54-55 0.0 0.0 0.0 0.5727422967463314 0.0 56-57 0.0 0.0 0.0 0.6594586531677218 0.0 58-59 0.0 0.0 0.0 0.7612689828911292 0.0 60-61 0.0 0.0 0.0 0.883737338820042 0.0 62-63 0.0 0.0 0.0 1.0033644762450455 0.0 64-65 0.0 0.0 0.0 1.1209790838964468 0.0 66-67 0.0 0.0 0.0 1.2435066318775245 0.0 68-69 0.0 0.0 0.0 1.3881128153160618 0.0 70-71 0.0 0.0 0.0 1.5635580579324453 0.0 72-73 0.0 0.0 0.0 1.7519663599729136 0.0 74-75 0.0 0.0 0.0 1.9376518276138026 0.0 76-77 0.0 0.0 0.0 2.131860950766419 0.0 78-79 0.0 0.0 0.0 2.355014987427608 0.0 80-81 0.0 0.0 0.0 2.610191924309939 0.0 82-83 0.0 0.0 0.0 2.893129933657548 0.0 84-85 0.0 0.0 0.0 3.172398035770941 0.0 86-87 0.0 0.0 0.0 3.4527907868754646 0.0 88-89 0.0 0.0 0.0 3.7712440275219365 0.0 90-91 2.3676820865908694E-4 0.0 0.0 4.136163029117754 0.0 92-93 2.3676820865908694E-4 0.0 0.0 4.528902295231015 0.0 94-95 2.3676820865908694E-4 0.0 0.0 4.9228845944397355 0.0 96-97 2.3676820865908694E-4 0.0 0.0 5.320477608830507 0.0 98-99 3.5515231298863037E-4 0.0 0.0 5.736183391183699 0.0 100-101 3.5515231298863037E-4 0.0 0.0 6.209483040293214 0.0 102-103 3.5515231298863037E-4 0.0 0.0 6.735463615829375 0.0 104-105 3.5515231298863037E-4 0.0 0.0 7.269198350199122 0.0 106-107 4.143443651534021E-4 0.0 0.0 7.794231852900648 0.0 108-109 4.735364173181739E-4 0.0 0.0 8.33151811040028 0.0 110-111 5.919205216477173E-4 0.0 0.0 8.922432367161196 0.0 112-113 5.919205216477173E-4 0.0 0.0 9.555787325324253 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2375 0.0 90.78329 1 GTATCAA 5325 0.0 65.65676 1 ATCAACG 7240 0.0 48.361168 3 TCAACGC 7325 0.0 47.881275 4 CAACGCA 7405 0.0 47.330338 5 TATCAAC 7405 0.0 47.205956 2 AACGCAG 7485 0.0 46.824467 6 ACGCAGA 8590 0.0 40.79382 7 TGGTATC 675 0.0 40.58241 2 CGCAGAG 8720 0.0 40.185658 8 GTGGTAT 750 0.0 38.11895 1 GCAGAGT 9595 0.0 35.962944 9 TATCTCG 35 0.0027883353 34.002537 26-27 GTACATG 6055 0.0 31.96908 1 GAGTACT 5975 0.0 31.016472 12-13 ACATGGG 6425 0.0 29.564842 3 CAGAGTA 9400 0.0 29.36719 10-11 TACATGG 6545 0.0 29.297459 2 AGAGTAC 9225 0.0 28.763433 10-11 AGTACTT 6300 0.0 28.519287 12-13 >>END_MODULE