FastQCFastQC Report
Fri 27 May 2016
SRR940148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101787
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG24350.11585379488977712No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG21870.10405431187841584No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21680.10315031922835188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42950.058.076921
GGTATCA24750.054.3608171
ATCAACG52750.045.9329833
TCAACGC53200.045.4325524
TATCAAC54750.045.016212
CAACGCA55200.043.6785965
AACGCAG56400.042.5219576
GTACATG105250.037.840491
TACATGG110050.035.270392
ACGCAGA68550.034.885987
CGCAGAG68600.034.8688478
ACATGGG109500.034.088363
CATGGGG57900.031.4626714
GCAGAGT79100.028.2091779
ATGGGGA31700.027.2308675
CATGGGA60900.026.8824064
TAAGGTA7300.025.2808444
GTGGTAT13150.023.9940091
GTCCAAG17000.023.8129221
GTGTAAG9800.023.0839561
Produced by FastQC (version 0.11.5)