FastQCFastQC Report
Fri 27 May 2016
SRR940148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101787
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51080.2430312871856187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39450.18769742128959785No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG26640.12674928525107443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23310.11090562459469012No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG22140.10533893301271728No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC21280.10124717680716457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.074.100181
GTATCAA74900.059.6560861
TATCAAC97150.046.039182
ATCAACG98200.045.301083
TCAACGC98900.045.0374454
CAACGCA101450.043.8185355
AACGCAG102950.043.234816
ACGCAGA121300.036.6365667
CGCAGAG123850.035.594038
GTACATG102100.035.1857571
TACATGG107350.032.5661622
ACATGGG107400.031.9941833
GTGGTAT17350.031.5910221
GCAGAGT137800.031.4294749
TAAGGTG11100.030.5591455
CATGGGG58100.030.3377954
GAGTACT77950.028.20027412-13
GTAAGGT10900.027.3156744
TGGTATC19300.026.8467942
CAGAGTA133250.026.45307210-11
Produced by FastQC (version 0.11.5)