FastQCFastQC Report
Fri 27 May 2016
SRR940147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923482
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17560.19014988922361237No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG11900.12886011855130908No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA10970.1187895378578034No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA9800.10612009763048982No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA9660.10460409623576854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.062.4872551
GTATCAA25950.062.378321
ATCAACG31500.049.6875233
TCAACGC32300.048.456874
TATCAAC33550.047.3612
CAACGCA33300.047.001715
AACGCAG34650.045.1631366
CCGTATG558.5261324E-443.2741859
GTACATG77050.040.549811
TACATGG79750.038.729232
ACATGGG81950.036.5276033
ACGCAGA43200.036.0868647
CGCAGAG43700.035.6739738
CATGGGG27300.033.5706864
GTGTAAG5150.033.511431
CATGGGA56650.032.2508134
GCAGAGT47450.031.4752459
ATGGGAG20750.030.6879485
AGGTAAG4600.029.7510016
TAAGGTA5050.028.2827764
Produced by FastQC (version 0.11.5)