FastQCFastQC Report
Fri 27 May 2016
SRR940147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923482
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68180.7382926792292649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55740.603585126726888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.37770091891341684No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12640.13687326878055012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.12842697529567443No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA10750.11640724995181281No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10640.11521610599881751No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA9850.10666152670003312No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA9390.10168037926023463No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG9370.10146380763241732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.081.021041
GTATCAA59750.065.0950851
ATCAACG77000.050.2636873
TCAACGC78150.049.524044
TATCAAC78350.049.397622
CAACGCA79100.048.8248755
GTGGTAT10150.048.1194571
AACGCAG81150.047.6647956
TGGTATC10350.045.4483832
ACGCAGA93650.040.9761127
CGCAGAG93550.040.9563188
GCAGAGT100900.037.7370229
GTACATG77100.036.0001641
TACATGG77350.035.718182
ACATGGG78950.034.5418053
CATGGGA53300.031.9499744
GAGTACT60750.031.3877812-13
CCCTATA2500.030.962432
CAGAGTA100450.030.05833410-11
TAGAGCG800.005358289529.753835
Produced by FastQC (version 0.11.5)