FastQCFastQC Report
Fri 27 May 2016
SRR940146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12769
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1761.3783381627378808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.4150677421881119No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.36807894118568407No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.29759573968204245No Hit
CCACAGGACAGTCCCATGTATCCAGTGGTTCCAGTTCACTTGGATGGGAC330.2584384055133527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.20361813767718695No Hit
GTACATGGGAGAAAAAAAAAACCTTGTAAAAAAAAGCAAAAACAACAACA250.19578667084344897No Hit
GTACATGGGATGACTGACGACCCTCCGACGACTAATCCACCGACTGCTCG250.19578667084344897No Hit
GTCCTGCACTAGCCTGGAAGTGCAGGAACAGTCTTCACTGCGGTCACTTG240.18795520400971102No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.14879786984102122No Hit
GGTATTTATTAATTAGAAAAGATCTATTCAGCAAAATTAAAGGGAGTAAG180.14096640300728325No Hit
GTACATGGGAGAACTCCACAGGACAGTCCCATGTATCCAGTGGTTCCAGT170.13313493617354533No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170.13313493617354533No Hit
GTGCTATATGGAGCGCAGACAGAGGAATTGTCTCTTCACTACTATGATCT170.13313493617354533No Hit
GATCTATTCAGCAAAATTAAAGGGAGTAAGTGACAGGTTTCACGAGGACA170.13313493617354533No Hit
CTTGTAAGGTGTCGAAGGTGGAACCCATTCATGAATCTTCTTTCTAGTGG160.12530346933980735No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.12530346933980735No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG160.12530346933980735No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG150.1174720025060694No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC150.1174720025060694No Hit
ATGCAAAGGTATTTATTAATTAGAAAAGATCTATTCAGCAAAATTAAAGG150.1174720025060694No Hit
GTCTTCACTGCGGTCACTTGTAAGGTGTCGAAGGTGGAACCCATTCATGA150.1174720025060694No Hit
GTACAGGGGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG140.10964053567233142No Hit
ACTATGATCTCGGCAGTAGCAGATGAGCAAAGGTGCAAAGTGCTGGCACT140.10964053567233142No Hit
GTACATGGGAACTAAGCTTTTGTCTAAGGCTGCTAGTCACCCTCATTTTT140.10964053567233142No Hit
ATTTTAATCAAGTTCATGGTCAGCTGCTAGGATGGTCCTGCACTAGCCTG140.10964053567233142No Hit
TATCAACGCAGAGTACATGGGAGAAAAAAAAAACCTTGTAAAAAAAAGCA130.10180906883859348No Hit
GTCTAAGGCTGCTAGTCACCCTCATTTTTCTTCAACAGAAAAATTAACAA130.10180906883859348No Hit
GTATGAGATGGTTAAAAAGGCCAAAGATAAAAAGATTTTTTTTTTCCTTT130.10180906883859348No Hit
TTCCAAGGCAGGTAAGATGGCTTTTGTGAGTAAAGGAGCTTGCTGCCCAA130.10180906883859348No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACA208.291234E-489.244122
GTAACAA208.291234E-489.244121
GGAAACA208.291234E-489.244121
GAACAAA300.00414231759.496081
ATATTAA255.236217E-447.596863108-109
TATCAAC1355.1257302E-835.2569351
ACAACAC750.003769643131.7312414
GCTGGCA400.005267337429.74804110-111
GTATCAA1004.201482E-429.748041
TAACAAA400.005267337429.7480478-79
GGTATCA800.005169754429.748041
CATGGGA953.4297045E-828.18235216-17
CAACACA850.006952502827.9981544
ATGGGAA656.1437146E-527.45972818-19
GCAGAGT1159.515875E-425.867869
AACGCAG1200.001219840524.7900335
CGCAGAG1200.001219840524.7900338
TCAACGC1300.001944395422.8831083
ACGCAGA1400.002990908221.24867
CAGAGTA1155.7644793E-620.6942910-11
Produced by FastQC (version 0.11.5)