FastQCFastQC Report
Fri 27 May 2016
SRR940146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12769
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT10608.301354843762237No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT4943.868744615866552RNA PCR Primer, Index 33 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3792.9681259299866865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3472.717518991307072No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT3212.513900853629885RNA PCR Primer, Index 33 (95% over 24bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG2702.114496045109249RNA PCR Primer, Index 33 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2421.8952149737645863No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT1511.182551491894432RNA PCR Primer, Index 33 (96% over 27bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG1030.8066410838750099RNA PCR Primer, Index 15 (95% over 24bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA920.7204949487038923RNA PCR Primer, Index 33 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.4698880100242776No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.4698880100242776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.4463936095230637No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT500.39157334168689795No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC480.37591040801942205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.37591040801942205No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT470.36807894118568407RNA PCR Primer, Index 33 (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.3524160075182082No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC450.3524160075182082No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC320.2506069386796147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.2506069386796147No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.2349440050121388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG290.22711253817840082No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC290.22711253817840082RNA PCR Primer, Index 15 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTT280.21928107134466285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC270.21144960451092487No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.21144960451092487No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTTCT250.19578667084344897Illumina PCR Primer Index 4 (95% over 21bp)
CCACAGGACAGTCCCATGTATCCAGTGGTTCCAGTTCACTTGGATGGGAC250.19578667084344897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA230.18012373717597308No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG220.1722922703422351RNA PCR Primer, Index 26 (100% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC220.1722922703422351No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC220.1722922703422351No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1722922703422351No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG210.16446080350849715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC210.16446080350849715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG200.15662933667475917No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT190.14879786984102122No Hit
GTACAGGGGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG180.14096640300728325No Hit
GTACATGGGAGAACTCCACAGGACAGTCCCATGTATCCAGTGGTTCCAGT180.14096640300728325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC180.14096640300728325No Hit
GTCCCATGTATCCAGTGGTTCCAGTTCACTTGGATGGGACATTTTAGCTC180.14096640300728325No Hit
GTGCTATATGGAGCGCAGACAGAGGAATTGTCTCTTCACTACTATGATCT180.14096640300728325No Hit
TTCCAAGGCAGGTAAGATGGCTTTTGTGAGTAAAGGAGCTTGCTGCCCAA170.13313493617354533No Hit
TTGTAGAACAACTCAGCAAAATAAAATTCCGGTTTATTGTTGGACATTGT170.13313493617354533No Hit
GTCCTGCACTAGCCTGGAAGTGCAGGAACAGTCTTCACTGCGGTCACTTG170.13313493617354533No Hit
ATCTTATTCCGAGCATTCCAGTAACTTTTGTGTATGTACCTAGCTGTACT170.13313493617354533No Hit
GATCTATTCAGCAAAATTAAAGGGAGTAAGTGACAGGTTTCACGAGGACA170.13313493617354533No Hit
TCCACCGACTGCTCGTAAATTCATCTGGACAAACCATAAATTCAATGTGT170.13313493617354533No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATTTCGTATGCCGT170.13313493617354533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG170.13313493617354533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC170.13313493617354533No Hit
AAATTAAAGGGAGTAAGTGACAGGTTTCACGAGGACATGTGCTATATGGA160.12530346933980735No Hit
GGTATTTATTAATTAGAAAAGATCTATTCAGCAAAATTAAAGGGAGTAAG160.12530346933980735No Hit
GCTATATGGAGCGCAGACAGAGGAATTGTCTCTTCACTACTATGATCTCG160.12530346933980735No Hit
ATGCAAAGGTATTTATTAATTAGAAAAGATCTATTCAGCAAAATTAAAGG160.12530346933980735No Hit
GTACATGGGAGAAAAAAAAAACCTTGTAAAAAAAAGCAAAAACAACAACA160.12530346933980735No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG150.1174720025060694No Hit
CTTGTAAGGTGTCGAAGGTGGAACCCATTCATGAATCTTCTTTCTAGTGG150.1174720025060694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG140.10964053567233142No Hit
ATCTCATACAAACCAACTACTTATAGTACAGCTAGGTACATACACAAAAG140.10964053567233142No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC140.10964053567233142No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG140.10964053567233142No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA140.10964053567233142RNA PCR Primer, Index 26 (100% over 29bp)
GTATGAGATGGTTAAAAAGGCCAAAGATAAAAAGATTTTTTTTTTCCTTT140.10964053567233142No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG140.10964053567233142No Hit
ATTTTAATCAAGTTCATGGTCAGCTGCTAGGATGGTCCTGCACTAGCCTG140.10964053567233142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC130.10180906883859348No Hit
ACTATGATCTCGGCAGTAGCAGATGAGCAAAGGTGCAAAGTGCTGGCACT130.10180906883859348No Hit
AATTAGAAAAGATCTATTCAGCAAAATTAAAGGGAGTAAGTGACAGGTTT130.10180906883859348No Hit
GTAGAATGATGGGTAGAGAAATAGCTTTTAAAACACATTCCAAGGCAGGT130.10180906883859348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC130.10180906883859348No Hit
GGTATCAACGCAGAGTACATGGGAGAACTCCACAGGACAGTCCCATGTAT130.10180906883859348No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA208.289063E-489.249996
TATAAGA208.289063E-489.249999
ATGTGTA208.289063E-489.249994
GTATAAG208.289063E-489.249998
AGATGTG208.289063E-489.249992
TGTATAA208.289063E-489.249997
GAGATGT208.289063E-489.249991
GATGTGT250.002010364871.43
GATGTAT150.004189269659.578-79
GGTATCA1300.054.9230771
GTATCAA2300.051.739131
CTTATAC3600.041.3194471
TATACAC3700.040.20273
TTATACA3850.038.6363642
TATCAAC3100.038.38712
GTGGTAT650.001867969336.6153831
ATACACA4200.035.4166644
TCAACGC3400.035.04
ATCAACG3400.035.03
TACACAT4350.034.1954045
Produced by FastQC (version 0.11.5)