##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940146_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12769 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.270890437778995 33.0 31.0 34.0 30.0 34.0 2 32.35594016759339 33.0 31.0 34.0 30.0 34.0 3 32.39658548046049 34.0 31.0 34.0 30.0 34.0 4 35.9658548046049 37.0 35.0 37.0 35.0 37.0 5 36.00438562142689 37.0 35.0 37.0 35.0 37.0 6 36.09421254600987 37.0 36.0 37.0 35.0 37.0 7 36.01018090688386 37.0 35.0 37.0 35.0 37.0 8 36.01440989897408 37.0 35.0 37.0 35.0 37.0 9 37.778526117941894 39.0 38.0 39.0 35.0 39.0 10-11 37.76814942438719 39.0 38.0 39.0 35.0 39.0 12-13 37.7277390555251 39.0 38.0 39.0 35.0 39.0 14-15 38.83495183647897 40.5 38.0 41.0 34.5 41.0 16-17 38.82993969770538 40.0 38.0 41.0 34.5 41.0 18-19 39.10051687681103 41.0 38.5 41.0 35.0 41.0 20-21 39.10701699428303 41.0 39.0 41.0 35.0 41.0 22-23 38.97231576474273 41.0 38.0 41.0 35.0 41.0 24-25 38.77758634192185 41.0 38.0 41.0 35.0 41.0 26-27 38.35950348500275 40.0 38.0 41.0 34.0 41.0 28-29 37.823126321560025 40.0 36.5 41.0 33.0 41.0 30-31 37.15706006735061 39.0 35.5 41.0 33.0 41.0 32-33 36.156668494008926 38.0 35.0 41.0 31.5 41.0 34-35 35.43781815334012 38.0 35.0 41.0 30.0 41.0 36-37 34.92188111833346 37.0 35.0 41.0 27.5 41.0 38-39 33.95947215913541 36.0 34.0 40.0 21.0 41.0 40-41 33.90300728326416 37.0 35.0 40.0 22.0 41.0 42-43 33.479403242227264 37.0 34.5 40.0 12.0 41.0 44-45 32.869136189208234 37.5 33.5 40.5 2.0 41.0 46-47 31.536690422116063 37.5 32.0 41.0 2.0 41.0 48-49 30.215639439266972 37.5 28.0 41.0 2.0 41.0 50-51 29.20890437778996 36.0 23.5 40.0 2.0 41.0 52-53 28.8858172135641 36.0 22.5 39.0 2.0 41.0 54-55 28.303469339807343 36.0 17.5 39.0 2.0 41.0 56-57 27.76446863497533 35.0 9.0 39.0 2.0 41.0 58-59 27.148993656511863 35.0 2.0 38.0 2.0 41.0 60-61 25.958101652439503 35.0 2.0 38.0 2.0 41.0 62-63 25.095700524708278 35.0 2.0 37.0 2.0 41.0 64-65 24.273044091158276 34.5 2.0 37.0 2.0 41.0 66-67 22.99913853864829 33.5 2.0 36.0 2.0 40.0 68-69 21.4748609914637 31.5 2.0 36.0 2.0 39.5 70-71 19.781032187328687 24.5 2.0 35.0 2.0 39.0 72-73 18.748179183961156 16.5 2.0 35.0 2.0 39.0 74-75 18.594525804683215 17.0 2.0 35.0 2.0 38.0 76-77 18.480499647583994 18.5 2.0 35.0 2.0 37.0 78-79 18.329117393687838 18.5 2.0 35.0 2.0 36.5 80-81 18.134936173545306 17.0 2.0 35.0 2.0 36.0 82-83 17.96444514057483 12.5 2.0 35.0 2.0 36.0 84-85 17.774962800532542 7.0 2.0 35.0 2.0 35.5 86-87 17.603806092881197 4.5 2.0 35.0 2.0 35.0 88-89 17.468713289999215 2.0 2.0 35.0 2.0 35.0 90-91 17.36369331975879 2.0 2.0 35.0 2.0 35.0 92-93 17.31020440128436 2.0 2.0 35.0 2.0 35.0 94-95 17.21607016994283 2.0 2.0 35.0 2.0 35.0 96-97 17.113477954420866 2.0 2.0 35.0 2.0 35.0 98-99 17.03590727543269 2.0 2.0 35.0 2.0 35.0 100-101 16.92912522515467 2.0 2.0 35.0 2.0 35.0 102-103 16.430769833189757 2.0 2.0 34.0 2.0 35.0 104-105 16.666888558226955 2.0 2.0 35.0 2.0 35.0 106-107 16.550356331740936 2.0 2.0 35.0 2.0 35.0 108-109 16.384877437544052 2.0 2.0 35.0 2.0 35.0 110-111 16.25742031482497 2.0 2.0 35.0 2.0 35.0 112-113 16.01370506695904 2.0 2.0 35.0 2.0 35.0 114-115 15.765604197666224 2.0 2.0 35.0 2.0 35.0 116-117 15.489466677108624 2.0 2.0 35.0 2.0 35.0 118-119 15.310752603962722 2.0 2.0 35.0 2.0 35.0 120-121 15.155141357976348 2.0 2.0 35.0 2.0 35.0 122-123 14.855783538256716 2.0 2.0 35.0 2.0 35.0 124-125 13.889654632312633 2.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0629825511581217 1101 2 0.5463522010970934 1101 3 0.9697179961508908 1101 4 0.9402481172566723 1101 5 0.8906536227538169 1101 6 0.6498466655196182 1101 7 1.0314653508996514 1101 8 0.9536462350893444 1101 9 1.2273341346238098 1101 10-11 1.1079292955052509 1101 12-13 1.165053309292439 1101 14-15 1.3187179738718413 1101 16-17 1.3953575466921393 1101 18-19 1.3278299961413467 1101 20-21 1.35055192857115 1101 22-23 1.0951271515807122 1101 24-25 0.8202493227523959 1101 26-27 0.924284350208282 1101 28-29 1.3371633944673604 1101 30-31 0.9572664346123787 1101 32-33 0.05337316192402852 1101 34-35 0.360299546915094 1101 36-37 0.4208595310930221 1101 38-39 2.7983771269298217 1101 40-41 1.0874147439897683 1101 42-43 -1.7482497559994137 1101 44-45 -1.9590217165615371 1101 46-47 -5.1137541190236355 1101 48-49 -4.328563982829547 1101 50-51 -2.2213552530013203 1101 52-53 -2.1054913853938935 1101 54-55 -3.2572220238670404 1101 56-57 -3.0116394272890155 1101 58-59 -2.223134710598835 1101 60-61 -0.7484864835029121 1101 62-63 0.4632395077834772 1101 64-65 -0.46451452363686485 1101 66-67 0.9165121496502309 1101 68-69 2.130650599086305 1101 70-71 3.4500023420100483 1101 72-73 4.207748143835541 1101 74-75 4.272767702267615 1101 76-77 4.402535898589683 1101 78-79 4.161020800888593 1101 80-81 4.470910618522861 1101 82-83 4.565081847150136 1101 84-85 4.3913584769929415 1101 86-87 4.509133981499282 1101 88-89 4.538320129642198 1101 90-91 3.807438859560623 1101 92-93 4.466614753664228 1101 94-95 4.542607511287528 1101 96-97 4.623048109453261 1101 98-99 4.646783431237818 1101 100-101 4.787115811010228 1101 102-103 5.130613667535966 1101 104-105 5.192508591292874 1101 106-107 5.068880484994903 1101 108-109 5.344818682707416 1101 110-111 5.389215783067737 1101 112-113 5.648209921960905 1101 114-115 5.856930032131556 1101 116-117 6.057399154110607 1101 118-119 6.373754757204933 1101 120-121 6.411549729829806 1101 122-123 6.6728400883439285 1101 124-125 5.902619144241557 1102 1 0.3571001982169406 1102 2 0.4287051422735644 1102 3 -0.08910553326087012 1102 4 0.23436576431549838 1102 5 0.6553595051067589 1102 6 0.7674937243431472 1102 7 0.44323005678200644 1102 8 0.48305799979522845 1102 9 0.16851060521204175 1102 10-11 0.6667528249170189 1102 12-13 -0.04082904364873485 1102 14-15 0.4657767974012472 1102 16-17 0.6306516643392044 1102 18-19 0.5337123490825206 1102 20-21 0.52702251680644 1102 22-23 0.5363036221689441 1102 24-25 0.555543440399461 1102 26-27 0.688990232561224 1102 28-29 0.6018692768203024 1102 30-31 1.4572664346123787 1102 32-33 1.6710202207475646 1102 34-35 1.007358370444507 1102 36-37 1.1267418840342032 1102 38-39 0.2101418328121767 1102 40-41 0.9109441557544784 1102 42-43 1.2811620087064668 1102 44-45 1.688037106967876 1102 46-47 4.150951763329314 1102 48-49 3.9655536642292866 1102 50-51 3.8080565117045566 1102 52-53 4.188626261664933 1102 54-55 4.625130917309434 1102 56-57 4.90012527859334 1102 58-59 5.453335877636462 1102 60-61 6.04563116355591 1102 62-63 6.404415978371713 1102 64-65 6.5943090057749 1102 66-67 7.06357097317964 1102 68-69 8.248297657909834 1102 70-71 6.567649400833577 1102 72-73 4.560689320306128 1102 74-75 4.625708878738202 1102 76-77 3.0495947221190924 1102 78-79 2.5433737420650644 1102 80-81 3.2944400302875643 1102 82-83 2.8003759647971975 1102 84-85 3.185476124051764 1102 86-87 2.773839863852224 1102 88-89 3.8324377767010205 1102 90-91 3.8368506242665035 1102 92-93 4.260732400723054 1102 94-95 3.572019275993412 1102 96-97 4.328930462394439 1102 98-99 4.087959901826057 1102 100-101 3.5224099286572894 1102 102-103 1.6600254322418468 1102 104-105 1.574861532469349 1102 106-107 1.833586367347845 1102 108-109 2.521289270942713 1102 110-111 2.565686371303034 1102 112-113 1.6776216866667895 1102 114-115 1.9157535615433172 1102 116-117 1.9691638599929586 1102 118-119 0.25610769838140435 1102 120-121 0.6174320827709838 1102 122-123 0.8198989118733433 1102 124-125 0.6967367913003812 1103 1 0.5335707864522377 1103 2 0.8992933775676804 1103 3 0.7932474079156009 1103 4 0.3520128231390274 1103 5 0.7730065639302879 1103 6 0.6498466655196182 1103 7 0.32558299795847745 1103 8 0.48305799979522845 1103 9 0.7567458993296938 1103 10-11 0.2549881190346568 1103 12-13 0.45917095635126515 1103 14-15 0.6422473856365372 1103 16-17 -0.31052480624903467 1103 18-19 0.06312411378840466 1103 20-21 0.9387872226887879 1103 22-23 0.8892447986395382 1103 24-25 0.437896381575932 1103 26-27 1.306637291384746 1103 28-29 1.4842222179967735 1103 30-31 1.3984429052006107 1103 32-33 1.4357261031005066 1103 34-35 2.33088778220921 1103 36-37 2.891447766387145 1103 38-39 2.2689653622239376 1103 40-41 2.7638853322250583 1103 42-43 4.134103185177061 1103 44-45 2.89391945990905 1103 46-47 4.5921282339175455 1103 48-49 3.9067301348175185 1103 50-51 4.396291805822209 1103 52-53 4.7768615557825775 1103 54-55 5.09571915260355 1103 56-57 5.3707135138874555 1103 58-59 6.512159407048223 1103 60-61 6.457395869438265 1103 62-63 6.992651272489361 1103 64-65 7.0943090057749 1103 66-67 6.53415920847376 1103 68-69 7.601238834380425 1103 70-71 8.71470822436299 1103 72-73 7.178336379129657 1103 74-75 6.919826525797024 1103 76-77 5.049594722119092 1103 78-79 5.778667859712122 1103 80-81 5.765028265581684 1103 82-83 3.9474347883266105 1103 84-85 3.7442996534635284 1103 86-87 4.126781040322811 1103 88-89 4.2442024825833755 1103 90-91 4.278027094854739 1103 92-93 4.260732400723054 1103 94-95 4.248489864228702 1103 96-97 4.446577521217968 1103 98-99 4.7350187253554665 1103 100-101 4.875351105127876 1103 102-103 5.130613667535966 1103 104-105 4.810155650116407 1103 106-107 5.068880484994903 1103 108-109 5.0801128003544775 1103 110-111 5.183333430126563 1103 112-113 5.70703345137267 1103 114-115 5.856930032131556 1103 116-117 5.85151680116943 1103 118-119 5.69728416896964 1103 120-121 6.323314435712158 1103 122-123 6.40813420599099 1103 124-125 6.284972085418028 1104 1 0.6512178452757666 1104 2 0.7228227893323904 1104 3 0.6167768196803038 1104 4 -1.2362224709786247 1104 5 -0.40346402430500916 1104 6 0.3557290184607922 1104 7 -0.8508875902768196 1104 8 -0.5169420002047715 1104 9 0.6390988405061648 1104 10-11 0.43145870726995383 1104 12-13 0.5474062504689101 1104 14-15 0.9951885621071312 1104 16-17 0.2777104878686174 1104 18-19 0.5043005843766366 1104 20-21 -1.384742189075915 1104 22-23 -0.9931081425369399 1104 24-25 -0.29739773607112596 1104 26-27 -2.487480355674073 1104 28-29 -2.1040130761208786 1104 30-31 -1.9250865065640923 1104 32-33 -2.7701562498406744 1104 34-35 -2.727935747202551 1104 36-37 -1.6379639983187388 1104 38-39 -4.554564049540765 1104 40-41 -3.9125852560102317 1104 42-43 -1.5423674030582362 1104 44-45 -1.7531393636203596 1104 46-47 -0.9372835307883385 1104 48-49 -0.005034571064836513 1104 50-51 -0.07429642947191084 1104 52-53 -0.3996090324527195 1104 54-55 -0.021927906219978865 1104 56-57 1.2530664550639266 1104 58-59 0.8062770541070492 1104 60-61 0.13386645767355532 1104 62-63 0.02206303719524172 1104 64-65 1.2119560645984286 1104 66-67 0.9459239143561113 1104 68-69 0.13065059908630516 1104 70-71 0.8911788125982874 1104 72-73 1.6195128497178928 1104 74-75 0.4198265257970242 1104 76-77 -0.626875866116201 1104 78-79 -0.6330968461702291 1104 80-81 0.2062047361699193 1104 82-83 -0.40550638814398 1104 84-85 -0.16746505241882303 1104 86-87 0.7444280991463401 1104 88-89 0.391261306112785 1104 90-91 1.101556506619442 1104 92-93 1.0548500477818799 1104 94-95 1.2484898642287021 1104 96-97 2.328930462394439 1104 98-99 0.7350187253554665 1104 100-101 0.7577040463043474 1104 102-103 0.3953195498889084 1104 104-105 0.8101556501164069 1104 106-107 0.480645190877258 1104 108-109 1.0801128003544775 1104 110-111 1.153921665420679 1104 112-113 1.70703345137267 1104 114-115 1.7392829733080273 1104 116-117 1.498575624698839 1104 118-119 2.2561076983814043 1104 120-121 2.0586085533592193 1104 122-123 1.8787224412851096 1104 124-125 -1.1267926204643253 1105 1 0.16886490409929422 1105 2 0.5139992599206238 1105 3 0.46677681968030527 1105 4 0.30789517608020134 1105 5 0.22594774040087628 1105 6 0.0027878419902052087 1105 7 0.03146535089965141 1105 8 -0.3787067060871294 1105 9 0.12733413462380838 1105 10-11 -0.9111883515535695 1105 12-13 0.10475919164537828 1105 14-15 0.25107091504830237 1105 16-17 0.7938869584568522 1105 18-19 0.1998888196707469 1105 20-21 0.2270225168064428 1105 22-23 0.861303622168947 1105 24-25 0.7731904992229843 1105 26-27 1.40957846785534 1105 28-29 1.3695163356438371 1105 30-31 0.8925605522594395 1105 32-33 0.13719669133580226 1105 34-35 1.625005429268036 1105 36-37 1.054683060504793 1105 38-39 0.04543595045922899 1105 40-41 -0.9772911383631708 1105 42-43 -2.210014461881766 1105 44-45 -0.8619628930321213 1105 46-47 -0.6372835307883378 1105 48-49 0.5729066054057519 1105 50-51 -1.7081199588836746 1105 52-53 -0.38049138539389205 1105 54-55 -0.10869261210233105 1105 56-57 -1.7219335449360749 1105 58-59 -2.5951935341282493 1105 60-61 -1.4720158952676172 1105 62-63 -1.5044075510400532 1105 64-65 -1.3159851118721555 1105 66-67 -2.7628996150556517 1105 68-69 -1.460525871501929 1105 70-71 -4.370585893284067 1105 72-73 -6.915781267929166 1105 74-75 -6.433114650673563 1105 76-77 -6.5945229249397315 1105 78-79 -6.424273316758466 1105 80-81 -6.168795263830081 1105 82-83 -5.8613887410851575 1105 84-85 -5.6159944641835295 1105 86-87 -5.455571900853659 1105 88-89 -5.220503399769566 1105 90-91 -5.136678787498203 1105 92-93 -5.0569146581004745 1105 94-95 -5.339745429888943 1105 96-97 -5.26371659642909 1105 98-99 -4.859098921703357 1105 100-101 -5.168766541930946 1105 102-103 -4.623798097169917 1105 104-105 -4.998667879295358 1105 106-107 -4.937001867946272 1105 108-109 -4.203710729057287 1105 110-111 -4.559313628696966 1105 112-113 -3.812084195686154 1105 114-115 -3.8327758502213864 1105 116-117 -3.699953787065864 1105 118-119 -3.538009948677418 1105 120-121 -4.407567917229015 1105 122-123 -3.8506893234207737 1105 124-125 -2.7062043851702082 1106 1 0.7688649040992956 1106 2 0.7889992599206224 1106 3 0.30427681968030384 1106 4 0.5578951760802013 1106 5 0.18844774040087486 1106 6 0.6277878419902052 1106 7 0.7814653508996514 1106 8 0.3837932939128734 1106 9 0.9773341346238098 1106 10-11 -0.27993835155357516 1106 12-13 0.3360091916453811 1106 14-15 0.2948209150483052 1106 16-17 -0.24986304154315064 1106 18-19 0.8498888196707526 1106 20-21 0.65202251680644 1106 22-23 0.3175536221689441 1106 24-25 0.92319049922299 1106 26-27 0.12832846785534002 1106 28-29 0.6257663356438314 1106 30-31 -0.5261894477405633 1106 32-33 0.3309466913357966 1106 34-35 0.593755429268036 1106 36-37 1.2921830605047901 1106 38-39 1.0391859504592347 1106 40-41 0.3852088616368263 1106 42-43 -0.8475144618817652 1106 44-45 -2.030712893032124 1106 46-47 -0.15603353078833848 1106 48-49 -2.9333433945942495 1106 50-51 -4.039369958883675 1106 52-53 -3.9492413853938935 1106 54-55 -3.152442612102334 1106 56-57 -3.3406835449360734 1106 58-59 -2.620193534128248 1106 60-61 -1.1970158952676186 1106 62-63 -1.6231575510400518 1106 64-65 -2.0784851118721583 1106 66-67 -1.6128996150556532 1106 68-69 -0.5605258715019303 1106 70-71 0.3231641067159323 1106 72-73 1.5717187320708348 1106 74-75 2.441885349326437 1106 76-77 2.81797707506027 1106 78-79 2.4257266832415354 1106 80-81 2.8312047361699193 1106 82-83 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36-37 -0.0657714849497566 2216 38-39 -1.668200413177125 2216 40-41 0.02725431618227958 2216 42-43 -1.591832643699945 2216 44-45 -0.19832652939576434 2216 46-47 1.8127164692116615 2216 48-49 2.438815696314844 2216 50-51 1.9577891320254182 2216 52-53 3.7126904327879195 2216 54-55 1.748125569715846 2216 56-57 3.1621573641548366 2216 58-59 2.220715556780842 2216 60-61 4.677984104732381 2216 62-63 1.391046994414495 2216 64-65 2.0862876154005683 2216 66-67 -0.47653597869201647 2216 68-69 0.5076559466798898 2216 70-71 1.4339595612613856 2216 72-73 2.0660369138890147 2216 74-75 1.7714308038718904 2216 76-77 1.4145679841511765 2216 78-79 1.334817592332442 2216 80-81 0.6152956452608258 2216 82-83 -0.15684328653970425 2216 84-85 -0.059176282365349664 2216 86-87 0.5626099173281602 2216 88-89 -0.11141249067865644 2216 90-91 -0.9184969693163829 2216 92-93 -1.6978237490095651 2216 94-95 -1.0897454298889429 2216 96-97 -1.7205347782472735 2216 98-99 -2.400008012612446 2216 100-101 -1.759675632840036 2216 102-103 -2.466979915351736 2216 104-105 -1.7259406065680842 2216 106-107 -1.046092777037181 2216 108-109 -1.183256183602742 2216 110-111 -1.343404537787876 2216 112-113 0.18564307704112082 2216 114-115 0.6149514225058876 2216 116-117 -0.09768105979313724 2216 118-119 0.5528991422316718 2216 120-121 1.0947048100437105 2216 122-123 0.3720379493064989 2216 124-125 -0.6334771124429341 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 6.0 6 10.0 7 39.0 8 87.0 9 129.0 10 195.0 11 223.0 12 290.0 13 426.0 14 719.0 15 753.0 16 392.0 17 338.0 18 341.0 19 507.0 20 508.0 21 734.0 22 673.0 23 113.0 24 81.0 25 128.0 26 155.0 27 128.0 28 182.0 29 184.0 30 187.0 31 196.0 32 226.0 33 276.0 34 354.0 35 642.0 36 1026.0 37 1749.0 38 770.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.79320068933104 20.867930440231866 16.559611467961773 22.779257402475324 2 13.12553841334482 21.841960999295168 45.563474038687445 19.469026548672566 3 13.988095238095239 33.53696741854637 40.327380952380956 12.147556390977442 4 7.713412244258054 27.929764051109196 27.772987379477932 36.58383632515482 5 7.244674185463659 37.94642857142857 36.270363408521305 18.538533834586467 6 19.016290726817044 43.17042606516291 14.653822055137844 23.159461152882205 7 13.08638107917613 41.65557208865221 17.76959824575143 27.48844858642024 8 24.073929046910486 34.71689247396037 22.10823087164226 19.10094760748688 9 20.98833111441773 12.295402928968596 16.751507557365493 49.964758399248176 10-11 20.162111363458376 29.08606782050278 25.099851202130157 25.651969613908687 12-13 26.110110423682354 26.544756832954814 25.929986686506385 21.41514605685645 14-15 22.315764742736313 26.1296890907667 26.53692536612107 25.017620800375912 16-17 17.894901715091237 31.76442947764116 26.928498707807975 23.41217009945963 18-19 17.644294776411623 22.014253269637404 37.78682747278565 22.554624481165323 20-21 17.906648915341844 19.582582817761768 40.1793405904926 22.331427676403788 22-23 17.07259769754875 26.384211762863185 40.0148797869841 16.528310752603964 24-25 17.714777977915265 27.35139791682982 40.813689404025375 14.120134701229539 26-27 17.683452110580312 29.05082621975096 42.01581956300415 11.249902106664578 28-29 15.780405669981986 23.55705223588378 43.4607251938288 17.201816900305428 30-31 20.782424812030076 22.023809523809522 43.03728070175439 14.156484962406015 32-33 16.908136894040254 23.72542877280915 45.49690657060068 13.869527762549927 34-35 16.097580076748375 18.9090766700603 47.03970553684705 17.95363771634427 36-37 13.442712820111208 24.55947999060224 43.961939071188034 18.03586811809852 38-39 15.122562455948 16.34427128201112 47.30205967577727 21.231106586263607 40-41 13.642415224371524 16.23854647975566 50.08614613517112 20.0328921607017 42-43 18.63889106429634 14.206280836400659 49.38914558696844 17.76568251233456 44-45 13.173043035595411 19.90836824999021 47.335239064886245 19.583349649528138 46-47 20.949994126169873 15.185808826408739 40.255315816266595 23.608881231154797 48-49 19.41029054741953 15.713838201895214 38.934137363928265 25.94173388675699 50-51 16.25812514684 19.128357741404965 39.57240191087791 25.041115200877123 52-53 15.565040332054195 22.186545539979637 42.68932571070562 19.559088417260554 54-55 14.296342704988643 23.75283890672723 36.37716344271282 25.573654945571306 56-57 19.95457749236432 26.854099772887462 29.567703030777665 23.62361970397055 58-59 12.687473078278577 32.18075733249795 37.0090456983984 18.12272389082508 60-61 17.017777429712584 36.9723549220769 27.19868431357193 18.811183334638578 62-63 12.74179653849166 41.22875714621348 27.3670608504973 18.662385464797556 64-65 12.801033794102676 39.92246544229941 28.268786466695385 19.007714296902535 66-67 12.808364006578433 39.474508575456184 27.641162189678127 20.075965228287256 68-69 13.68602420018013 37.455456788189686 28.445001370560362 20.41351764106982 70-71 14.920112781954886 34.140037593984964 29.209743107769427 21.730106516290725 72-73 15.397266711046717 31.777420996984766 30.41469240709559 22.41061988487293 74-75 17.209648367139167 29.301433158430573 30.444827316156314 23.044091158273943 76-77 17.166575299553607 29.877045970710313 29.344506226016133 23.611872503719948 78-79 16.915968360873993 29.136972354922076 30.758085989505833 23.188973294698098 80-81 16.559636619938914 28.455634740386877 30.62886678674916 24.355861852925052 82-83 17.084344897799358 28.3890672723001 29.736079567703033 24.790508262197513 84-85 17.91839611559245 28.216775001957867 29.234865690343803 24.62996319210588 86-87 18.060069702784194 27.30156243881427 29.142029212515176 25.49633864588636 88-89 18.47515369855504 27.520852096957356 28.194384618396835 25.80960958609077 90-91 19.396146616541355 27.631578947368425 27.306547619047617 25.665726817042607 92-93 18.656015037593985 27.61983082706767 27.192982456140353 26.531171679197996 94-95 19.14793640848931 27.39447098441538 27.386639517581646 26.070953089513665 96-97 19.30216165413534 27.631578947368425 27.31437969924812 25.75187969924812 98-99 19.086032031953636 28.033833261542075 27.31722598582449 25.5629087206798 100-101 19.364818295739347 27.24389097744361 27.275219298245613 26.11607142857143 102-103 18.53708199545775 28.596601143394157 26.736627770381393 26.1296890907667 104-105 19.10094760748688 29.054741953167827 26.29023416085833 25.55407627848696 106-107 18.928655337144647 29.223118490093192 26.72488057013079 25.123345602631375 108-109 18.899549637752106 29.89622087331114 26.72410417074603 24.48012531819072 110-111 18.52925052862401 30.922546793014334 26.333307228443886 24.21489544991777 112-113 17.887770685671768 31.644280847397894 26.600618710106904 23.867329756823434 114-115 18.470514527370977 31.196648132195158 26.415537630198138 23.917299710235724 116-117 17.851828647505677 31.615631607800143 26.701386169629572 23.831153575064608 118-119 17.836165713838202 31.224058266113243 27.69598245751429 23.24379356253426 120-121 18.196413188190146 31.133996397525255 26.98331897564414 23.686271438640457 122-123 18.05615381603164 31.060813721267184 27.047029799898187 23.83600266280299 124-125 17.307014451885795 32.47170328594368 26.941604981788274 23.27967728038225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 61.0 1 42.5 2 30.0 3 34.5 4 21.5 5 14.5 6 27.0 7 61.5 8 87.0 9 94.5 10 69.0 11 33.0 12 28.0 13 29.0 14 38.0 15 45.5 16 43.5 17 46.5 18 44.0 19 47.0 20 63.0 21 73.0 22 84.0 23 86.0 24 94.0 25 107.5 26 127.5 27 158.0 28 211.5 29 282.0 30 315.5 31 340.0 32 359.5 33 332.5 34 344.5 35 351.5 36 312.0 37 331.0 38 369.5 39 374.5 40 390.0 41 419.0 42 458.5 43 509.5 44 472.0 45 426.5 46 448.5 47 453.5 48 527.0 49 521.5 50 468.0 51 457.5 52 334.0 53 216.5 54 159.0 55 125.5 56 96.0 57 90.5 58 95.0 59 91.0 60 91.0 61 90.5 62 81.0 63 53.0 64 35.0 65 25.0 66 13.0 67 9.5 68 10.0 69 4.5 70 3.5 71 4.0 72 1.5 73 1.0 74 1.0 75 0.5 76 1.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023494400501213878 2 0.0 3 0.00783146683373796 4 0.09397760200485551 5 0.00783146683373796 6 0.00783146683373796 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.00783146683373796 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.00391573341686898 46-47 0.00391573341686898 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.00391573341686898 60-61 0.0 62-63 0.0 64-65 0.00391573341686898 66-67 0.0 68-69 0.00391573341686898 70-71 0.00783146683373796 72-73 0.00391573341686898 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.00391573341686898 88-89 0.00391573341686898 90-91 0.00783146683373796 92-93 0.00783146683373796 94-95 0.0 96-97 0.00783146683373796 98-99 0.00391573341686898 100-101 0.00783146683373796 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.011747200250606939 110-111 0.0 112-113 0.00391573341686898 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.00391573341686898 124-125 0.019578667084344897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 12769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.023102827159526 #Duplication Level Percentage of deduplicated Percentage of total 1 83.47538702383022 37.583209335108464 2 7.392590015654897 6.656746808677265 3 2.9744303357105584 4.017542485707573 4 1.5828839798225778 2.850653927480617 5 0.9740824491215864 2.1928107134466286 6 0.9392937902243869 2.537395254131099 7 0.38267524786919466 1.2060458923956459 8 0.31309793007479564 1.127731224058266 9 0.27830927117759613 1.127731224058266 >10 1.4611236736823796 12.224919727464954 >50 0.06957731779439903 2.106664578275511 >100 0.13915463558879806 18.067193985433473 >500 0.0 0.0 >1k 0.017394329448599758 8.301354843762237 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 1060 8.301354843762237 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 494 3.868744615866552 RNA PCR Primer, Index 33 (95% over 22bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 379 2.9681259299866865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 347 2.717518991307072 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 321 2.513900853629885 RNA PCR Primer, Index 33 (95% over 24bp) TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 270 2.114496045109249 RNA PCR Primer, Index 33 (96% over 29bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 242 1.8952149737645863 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 151 1.182551491894432 RNA PCR Primer, Index 33 (96% over 27bp) CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 103 0.8066410838750099 RNA PCR Primer, Index 15 (95% over 24bp) CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA 92 0.7204949487038923 RNA PCR Primer, Index 33 (96% over 29bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.4698880100242776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.4698880100242776 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57 0.4463936095230637 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT 50 0.39157334168689795 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 48 0.37591040801942205 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.37591040801942205 No Hit TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT 47 0.36807894118568407 RNA PCR Primer, Index 33 (95% over 21bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45 0.3524160075182082 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC 45 0.3524160075182082 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 32 0.2506069386796147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 32 0.2506069386796147 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.2349440050121388 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 29 0.22711253817840082 No Hit ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC 29 0.22711253817840082 RNA PCR Primer, Index 15 (96% over 25bp) ATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTT 28 0.21928107134466285 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 27 0.21144960451092487 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27 0.21144960451092487 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTTCT 25 0.19578667084344897 Illumina PCR Primer Index 4 (95% over 21bp) CCACAGGACAGTCCCATGTATCCAGTGGTTCCAGTTCACTTGGATGGGAC 25 0.19578667084344897 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 23 0.18012373717597308 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 22 0.1722922703422351 RNA PCR Primer, Index 26 (100% over 29bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 22 0.1722922703422351 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC 22 0.1722922703422351 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.1722922703422351 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 21 0.16446080350849715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 21 0.16446080350849715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 20 0.15662933667475917 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 19 0.14879786984102122 No Hit GTACAGGGGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG 18 0.14096640300728325 No Hit GTACATGGGAGAACTCCACAGGACAGTCCCATGTATCCAGTGGTTCCAGT 18 0.14096640300728325 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 18 0.14096640300728325 No Hit GTCCCATGTATCCAGTGGTTCCAGTTCACTTGGATGGGACATTTTAGCTC 18 0.14096640300728325 No Hit GTGCTATATGGAGCGCAGACAGAGGAATTGTCTCTTCACTACTATGATCT 18 0.14096640300728325 No Hit TTCCAAGGCAGGTAAGATGGCTTTTGTGAGTAAAGGAGCTTGCTGCCCAA 17 0.13313493617354533 No Hit TTGTAGAACAACTCAGCAAAATAAAATTCCGGTTTATTGTTGGACATTGT 17 0.13313493617354533 No Hit GTCCTGCACTAGCCTGGAAGTGCAGGAACAGTCTTCACTGCGGTCACTTG 17 0.13313493617354533 No Hit ATCTTATTCCGAGCATTCCAGTAACTTTTGTGTATGTACCTAGCTGTACT 17 0.13313493617354533 No Hit GATCTATTCAGCAAAATTAAAGGGAGTAAGTGACAGGTTTCACGAGGACA 17 0.13313493617354533 No Hit TCCACCGACTGCTCGTAAATTCATCTGGACAAACCATAAATTCAATGTGT 17 0.13313493617354533 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATTTCGTATGCCGT 17 0.13313493617354533 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 17 0.13313493617354533 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 17 0.13313493617354533 No Hit AAATTAAAGGGAGTAAGTGACAGGTTTCACGAGGACATGTGCTATATGGA 16 0.12530346933980735 No Hit GGTATTTATTAATTAGAAAAGATCTATTCAGCAAAATTAAAGGGAGTAAG 16 0.12530346933980735 No Hit GCTATATGGAGCGCAGACAGAGGAATTGTCTCTTCACTACTATGATCTCG 16 0.12530346933980735 No Hit ATGCAAAGGTATTTATTAATTAGAAAAGATCTATTCAGCAAAATTAAAGG 16 0.12530346933980735 No Hit GTACATGGGAGAAAAAAAAAACCTTGTAAAAAAAAGCAAAAACAACAACA 16 0.12530346933980735 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 15 0.1174720025060694 No Hit CTTGTAAGGTGTCGAAGGTGGAACCCATTCATGAATCTTCTTTCTAGTGG 15 0.1174720025060694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 14 0.10964053567233142 No Hit ATCTCATACAAACCAACTACTTATAGTACAGCTAGGTACATACACAAAAG 14 0.10964053567233142 No Hit GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC 14 0.10964053567233142 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 14 0.10964053567233142 No Hit CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA 14 0.10964053567233142 RNA PCR Primer, Index 26 (100% over 29bp) GTATGAGATGGTTAAAAAGGCCAAAGATAAAAAGATTTTTTTTTTCCTTT 14 0.10964053567233142 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 14 0.10964053567233142 No Hit ATTTTAATCAAGTTCATGGTCAGCTGCTAGGATGGTCCTGCACTAGCCTG 14 0.10964053567233142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 13 0.10180906883859348 No Hit ACTATGATCTCGGCAGTAGCAGATGAGCAAAGGTGCAAAGTGCTGGCACT 13 0.10180906883859348 No Hit AATTAGAAAAGATCTATTCAGCAAAATTAAAGGGAGTAAGTGACAGGTTT 13 0.10180906883859348 No Hit GTAGAATGATGGGTAGAGAAATAGCTTTTAAAACACATTCCAAGGCAGGT 13 0.10180906883859348 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 13 0.10180906883859348 No Hit GGTATCAACGCAGAGTACATGGGAGAACTCCACAGGACAGTCCCATGTAT 13 0.10180906883859348 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.00391573341686898 0.0 22-23 0.0 0.0 0.0 0.0391573341686898 0.0 24-25 0.0 0.0 0.0 0.07439893492051061 0.0 26-27 0.0 0.0 0.0 0.14879786984102122 0.0 28-29 0.0 0.0 0.0 0.2701856057639596 0.0 30-31 0.0 0.0 0.0 0.5090453441929673 0.0 32-33 0.0 0.0 0.0 0.7557365494557131 0.0 34-35 0.0 0.0 0.0 1.0455008223040174 0.0 36-37 0.0 0.0 0.0 1.546714699663247 0.0 38-39 0.0 0.0 0.0 2.177147779779153 0.0 40-41 0.0 0.0 0.0 2.862401127731224 0.0 42-43 0.0 0.0 0.0 3.30096327042055 0.0 44-45 0.0 0.0 0.0 3.7395254131098756 0.0 46-47 0.0 0.0 0.0 4.174171822382332 0.0 48-49 0.0 0.0 0.0 4.542250763568016 0.0 50-51 0.0 0.0 0.0 4.961234239172997 0.0 52-53 0.0 0.0 0.0 5.290155846189991 0.0 54-55 0.0 0.0 0.0 5.7208865220455785 0.0 56-57 0.0 0.0 0.0 6.1281227973999535 0.0 58-59 0.0 0.0 0.0 6.5040332054193755 0.0 60-61 0.0 0.0 0.0 6.860364946354452 0.0 62-63 0.0 0.0 0.0 7.232359620957006 0.0 64-65 0.0 0.0 0.0 7.55736549455713 0.0 66-67 0.0 0.0 0.0 7.878455634740387 0.0 68-69 0.0 0.0 0.0 8.414911112851438 0.0 70-71 0.0 0.0 0.0 8.841726055290156 0.0 72-73 0.0 0.0 0.0 9.342939932649386 0.0 74-75 0.0 0.0 0.0 9.75409194142063 0.0 76-77 0.0 0.0 0.0 10.079097815020752 0.0 78-79 0.0 0.0 0.0 10.537238624794423 0.0 80-81 0.0 0.0 0.0 11.065862636071735 0.0 82-83 0.0 0.0 0.0 11.594486647349047 0.0 84-85 0.0 0.0 0.0 12.095700524708278 0.0 86-87 0.0 0.0 0.0 12.612577335734983 0.0 88-89 0.0 0.0 0.0 13.211684548515937 0.0 90-91 0.0 0.0 0.0 13.720729892708905 0.0 92-93 0.0 0.0 0.0 14.225859503485003 0.0 94-95 0.0 0.0 0.0 14.770146448429792 0.0 96-97 0.0 0.0 0.0 15.42798966246378 0.0 98-99 0.0 0.0 0.0 15.944866473490485 0.0 100-101 0.0 0.0 0.0 16.645782755110034 0.0 102-103 0.0 0.0 0.0 17.46417103923565 0.0 104-105 0.0 0.0 0.0 18.098519852768423 0.0 106-107 0.0 0.0 0.0 18.705458532383116 0.0 108-109 0.0 0.0 0.0 19.20275667632548 0.0 110-111 0.0 0.0 0.0 19.71963348735218 0.0 112-113 0.0 0.0 0.0 20.389223901636775 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTATA 20 8.289063E-4 89.24999 6 TATAAGA 20 8.289063E-4 89.24999 9 ATGTGTA 20 8.289063E-4 89.24999 4 GTATAAG 20 8.289063E-4 89.24999 8 AGATGTG 20 8.289063E-4 89.24999 2 TGTATAA 20 8.289063E-4 89.24999 7 GAGATGT 20 8.289063E-4 89.24999 1 GATGTGT 25 0.0020103648 71.4 3 GATGTAT 15 0.0041892696 59.5 78-79 GGTATCA 130 0.0 54.923077 1 GTATCAA 230 0.0 51.73913 1 CTTATAC 360 0.0 41.319447 1 TATACAC 370 0.0 40.2027 3 TTATACA 385 0.0 38.636364 2 TATCAAC 310 0.0 38.3871 2 GTGGTAT 65 0.0018679693 36.615383 1 ATACACA 420 0.0 35.416664 4 TCAACGC 340 0.0 35.0 4 ATCAACG 340 0.0 35.0 3 TACACAT 435 0.0 34.195404 5 >>END_MODULE