##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940145_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 108272 Sequences flagged as poor quality 0 Sequence length 125 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.483051943254026 31.0 16.0 34.0 7.0 34.0 2 25.829983744643123 31.0 20.0 34.0 7.0 34.0 3 26.778022018619772 31.0 25.0 34.0 7.0 34.0 4 28.347569085266734 35.0 19.0 37.0 7.0 37.0 5 30.41315390867445 35.0 31.0 37.0 10.0 37.0 6 28.763918649327618 37.0 22.0 37.0 8.0 37.0 7 29.321800650214275 36.0 27.0 37.0 8.0 37.0 8 28.470721885621398 36.0 19.0 37.0 7.0 37.0 9 29.15010344318014 38.0 17.0 39.0 7.0 39.0 10-11 30.02827139057189 38.0 24.5 39.0 7.0 39.0 12-13 30.050747192256537 38.0 25.5 39.0 7.0 39.0 14-15 31.088203783064873 39.0 27.0 41.0 7.0 41.0 16-17 31.539252992463425 39.0 30.0 41.0 7.0 41.0 18-19 31.45300724102261 38.0 30.0 41.0 2.0 41.0 20-21 31.352330242352593 38.0 28.0 41.0 2.0 41.0 22-23 31.29428661149697 38.0 27.5 41.0 2.0 41.0 24-25 31.19408526673563 38.0 27.0 41.0 2.0 41.0 26-27 31.072359428106992 38.0 26.5 41.0 2.0 41.0 28-29 30.94384974878085 37.5 25.0 41.0 2.0 41.0 30-31 30.777707994680064 37.0 25.0 41.0 2.0 41.0 32-33 30.617250997487808 36.0 25.0 41.0 2.0 41.0 34-35 30.477468782325992 36.0 25.0 41.0 2.0 41.0 36-37 30.268702896409046 35.0 25.0 41.0 2.0 41.0 38-39 30.073772535835673 35.0 25.0 41.0 2.0 41.0 40-41 29.947285540121175 35.0 25.0 41.0 2.0 41.0 42-43 29.800382370326588 35.0 25.0 40.5 2.0 41.0 44-45 29.773399401507312 35.0 25.0 41.0 2.0 41.0 46-47 29.63589385990838 35.0 25.0 41.0 2.0 41.0 48-49 29.47955149992611 35.0 25.0 41.0 2.0 41.0 50-51 29.357299209398555 35.0 25.0 40.0 2.0 41.0 52-53 29.1873614600266 35.0 25.0 40.0 2.0 41.0 54-55 29.05306542781144 35.0 25.0 40.0 2.0 41.0 56-57 28.911200495049506 35.0 25.0 40.0 2.0 41.0 58-59 28.652375498743904 35.0 25.0 40.0 2.0 41.0 60-61 28.407954041672824 35.0 25.0 39.5 2.0 41.0 62-63 28.14215124870696 35.0 25.0 39.0 2.0 41.0 64-65 27.88385270430028 34.5 25.0 39.0 2.0 41.0 66-67 27.61334417023792 34.0 25.0 38.0 2.0 41.0 68-69 27.347158083345647 34.0 25.0 37.0 2.0 41.0 70-71 27.241530589626127 34.0 25.0 37.0 2.0 40.5 72-73 27.064379525639133 34.0 25.0 36.5 2.0 40.0 74-75 26.809673784542632 34.0 25.0 36.0 2.0 39.0 76-77 26.551019654204225 34.0 24.5 36.0 2.0 39.0 78-79 26.323500073887985 34.0 24.0 35.0 2.0 38.5 80-81 26.087294037239545 34.0 24.0 35.0 2.0 37.0 82-83 25.859515848972958 34.0 24.0 35.0 2.0 37.0 84-85 25.651438044923896 33.5 23.0 35.0 2.0 36.5 86-87 25.453016477020835 33.0 21.0 35.0 2.0 36.0 88-89 25.280922122062954 33.0 20.0 35.0 2.0 36.0 90-91 25.124625942071816 33.0 17.5 35.0 2.0 36.0 92-93 24.968823887985813 33.0 12.5 35.0 2.0 35.0 94-95 24.8485942810699 33.0 6.0 35.0 2.0 35.0 96-97 24.7287525860795 33.0 2.0 35.0 2.0 35.0 98-99 24.63165915472144 33.0 2.0 35.0 2.0 35.0 100-101 24.535775639131078 33.0 2.0 35.0 2.0 35.0 102-103 23.909103923452047 30.5 2.0 34.5 2.0 35.0 104-105 24.317450495049506 32.5 2.0 35.0 2.0 35.0 106-107 24.321657492241762 33.0 2.0 35.0 2.0 35.0 108-109 24.25510750701936 33.0 2.0 35.0 2.0 35.0 110-111 24.149540047288312 32.0 2.0 35.0 2.0 35.0 112-113 24.035632481158565 32.0 2.0 35.0 2.0 35.0 114-115 23.89325495049505 32.0 2.0 35.0 2.0 35.0 116-117 23.781841103886507 32.0 2.0 35.0 2.0 35.0 118-119 23.649419979311364 31.5 2.0 35.0 2.0 35.0 120-121 23.471987217378455 31.0 2.0 35.0 2.0 35.0 122-123 23.259979496083936 31.0 2.0 35.0 2.0 35.0 124-125 21.907196689818235 28.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19444657112991237 1101 2 0.10971636442933885 1101 3 -0.7592296935781668 1101 4 -0.29183292203040523 1101 5 -0.2084915704866006 1101 6 -0.8040802183077744 1101 7 -0.45070240183391164 1101 8 -0.10229137322509985 1101 9 -0.5422838643443342 1101 10-11 -0.6359707249295674 1101 12-13 -0.683653622394953 1101 14-15 -0.9417865248968553 1101 16-17 -0.3615099624485332 1101 18-19 -0.2356475658162367 1101 20-21 -0.5051995906257325 1101 22-23 -0.4759565971902795 1101 24-25 -1.0715023161378348 1101 26-27 -0.7700950887680271 1101 28-29 -1.1838130614757247 1101 30-31 -0.8768574901585282 1101 32-33 -1.03336462239891 1101 34-35 -0.9824182702059368 1101 36-37 -0.7427529637290853 1101 38-39 -0.8295099794043601 1101 40-41 -1.071067237284847 1101 42-43 -1.2167761936710164 1101 44-45 -0.9844264634058995 1101 46-47 -0.9079434049178872 1101 48-49 -0.6918603744599459 1101 50-51 -1.1113659325987904 1101 52-53 -1.0198393927188825 1101 54-55 -1.599518053543754 1101 56-57 -1.494487066292404 1101 58-59 -1.5824033437424774 1101 60-61 -1.5343796817316928 1101 62-63 -1.385003242453891 1101 64-65 -1.1136544563032658 1101 66-67 -0.8863050189116493 1101 68-69 -1.050106733833701 1101 70-71 -1.3890300969706146 1101 72-73 -1.570095337041316 1101 74-75 -1.5124412272437482 1101 76-77 -1.3722256273196578 1101 78-79 -1.6063133329511103 1101 80-81 -1.5136046689688705 1101 82-83 -1.5781035275287323 1101 84-85 -1.3202749317353195 1101 86-87 -1.3098631223683626 1101 88-89 -1.2685369675290694 1101 90-91 -1.4882717752649128 1101 92-93 -1.154404945577717 1101 94-95 -1.253717687310946 1101 96-97 -1.1035024828183069 1101 98-99 -1.0155191401179842 1101 100-101 -1.2096063144015048 1101 102-103 -1.4204657318245886 1101 104-105 -1.814105278185611 1101 106-107 -1.823632059870988 1101 108-109 -1.739278034904956 1101 110-111 -1.6397402768402678 1101 112-113 -1.633100168157025 1101 114-115 -1.2952230333765264 1101 116-117 -1.3353659660646997 1101 118-119 -1.5057520320841213 1101 120-121 -1.5569769436192935 1101 122-123 -1.9375895549146591 1101 124-125 -1.5794422545594777 1102 1 0.9064676439268489 1102 2 0.7437680885672684 1102 3 0.7161055554639795 1102 4 0.5760789553642311 1102 5 0.28537816131416704 1102 6 0.21603472422096104 1102 7 0.32793744490938437 1102 8 1.164087937119728 1102 9 0.7152352927437882 1102 10-11 0.581605903422922 1102 12-13 0.18904752703033267 1102 14-15 1.1602728620763223 1102 16-17 1.1364067042181283 1102 18-19 1.1514453460611591 1102 20-21 1.2426691833206256 1102 22-23 1.1654227131545518 1102 24-25 1.1577601359694434 1102 26-27 1.637208551078718 1102 28-29 1.696191727796304 1102 30-31 1.984588869994731 1102 32-33 1.9823202435014728 1102 34-35 1.7892292393726095 1102 36-37 1.9563849673054001 1102 38-39 2.285288871170355 1102 40-41 2.2637028776576855 1102 42-43 2.351087791003316 1102 44-45 2.464831199429348 1102 46-47 2.5933975912506924 1102 48-49 2.5815112730496352 1102 50-51 2.483150350926117 1102 52-53 2.640289917625946 1102 54-55 2.365735777873873 1102 56-57 2.184966956696101 1102 58-59 1.9852211773303239 1102 60-61 2.1796049925978096 1102 62-63 2.0882247268947722 1102 64-65 2.1287066548078464 1102 66-67 2.1769852109734096 1102 68-69 2.2784133811088303 1102 70-71 2.325337719121343 1102 72-73 2.0569640499318638 1102 74-75 1.9780999604957117 1102 76-77 1.5190818439447114 1102 78-79 1.5087968203055944 1102 80-81 1.564604143291664 1102 82-83 1.3674903422030695 1102 84-85 1.3802997809083593 1102 86-87 1.3508409006201454 1102 88-89 1.5827082431989048 1102 90-91 1.3415653894860426 1102 92-93 1.1810117210889501 1102 94-95 1.6727190942982517 1102 96-97 1.9918279769518037 1102 98-99 1.6868036568168847 1102 100-101 1.6914952181655458 1102 102-103 1.7243858007424606 1102 104-105 1.7583324612779911 1102 106-107 1.680031733232461 1102 108-109 1.5987008922981083 1102 110-111 1.9224244741175838 1102 112-113 1.8607695636437427 1102 114-115 1.6179953574280717 1102 116-117 1.5411187082648041 1102 118-119 1.7484864736630072 1102 120-121 1.5332768877983334 1102 122-123 1.5086748129014325 1102 124-125 0.9151458680458866 1103 1 -0.7624978733145298 1103 2 -0.1994503022373273 1103 3 0.5110480841996115 1103 4 0.03538930019181663 1103 5 0.27595287395784496 1103 6 -0.11408021830777315 1103 7 -1.047924624056133 1103 8 -0.8306247065584316 1103 9 -0.048672753233223176 1103 10-11 0.40930705284820945 1103 12-13 -0.0786536223949561 1103 14-15 0.8834912528809191 1103 16-17 0.8597400375514646 1103 18-19 0.38282465640598673 1103 20-21 0.8491059649298229 1103 22-23 0.5673767361430571 1103 24-25 0.9148865727510547 1103 26-27 0.5955993556764163 1103 28-29 1.0035480496353912 1103 30-31 0.9695313987303606 1103 32-33 0.4676075998233138 1103 34-35 0.4098039520162864 1103 36-37 0.408913702937582 1103 38-39 0.426323353928975 1103 40-41 0.3205994293818222 1103 42-43 0.04016825077342645 1103 44-45 0.21460131437187613 1103 46-47 0.35316770619322213 1103 48-49 0.2670285144289437 1103 50-51 -0.13317148815434265 1103 52-53 -0.4810893927188822 1103 54-55 -0.3510458313215281 1103 56-57 -0.31032039962573776 1103 58-59 -0.21018112152025026 1103 60-61 -0.23660190395391467 1103 62-63 0.1630523131016659 1103 64-65 -0.037960011858821474 1103 66-67 0.2007783144216866 1103 68-69 -0.0380234005003679 1103 70-71 -0.04569676363727737 1103 72-73 0.05351577406979402 1103 74-75 0.22269766164514238 1103 76-77 0.41402437268034475 1103 78-79 0.6813255559377787 1103 80-81 0.5000064421422401 1103 82-83 0.1330075835823834 1103 84-85 0.22639173493134734 1103 86-87 0.022220210964974285 1103 88-89 0.16201858802648772 1103 90-91 0.3314504469573052 1103 92-93 0.1876783877556143 1103 94-95 0.1562823126890578 1103 96-97 0.14608085051502329 1103 98-99 0.1012864154375741 1103 100-101 0.010115907820715364 1103 102-103 -0.27032684293569886 1103 104-105 -0.39396638929672534 1103 106-107 -0.5790487265376569 1103 108-109 -0.12233359046051362 1103 110-111 -0.15251805461804935 1103 112-113 -0.3119890570459134 1103 114-115 0.00811029995680812 1103 116-117 -0.031754854953589984 1103 118-119 -0.04450203208411807 1103 120-121 0.10982861193626192 1103 122-123 -0.05592288824799141 1103 124-125 0.46916885655163654 1104 1 1.132866365096067 1104 2 1.0670839140982125 1104 3 1.4491496294534727 1104 4 0.952740293569299 1104 5 1.3099043088364297 1104 6 1.3509970443853838 1104 7 1.7037310050829397 1104 8 1.6220463751192717 1104 9 1.380775370387088 1104 10-11 1.098070849757704 1104 12-13 0.8480792694372745 1104 14-15 1.0455663080685618 1104 16-17 1.0383934591850128 1104 18-19 1.0948114113728735 1104 20-21 1.485441505768673 1104 22-23 1.3154341312865405 1104 24-25 1.4364539016693776 1104 26-27 1.408137987022993 1104 28-29 1.4533052240283197 1104 30-31 1.5494210234544212 1104 32-33 1.4826627874612832 1104 34-35 1.3137553868948757 1104 36-37 0.9368827978603171 1104 38-39 1.038177658564738 1104 40-41 1.235500091633476 1104 42-43 1.2725082066233213 1104 44-45 1.2307823298244145 1104 46-47 0.9123288540960921 1104 48-49 0.9470285144289434 1104 50-51 1.0040912050023891 1104 52-53 1.116129150327474 1104 54-55 1.1437444556541863 1104 56-57 1.4932336842594722 1104 58-59 1.3854038674422178 1104 60-61 1.5794908112778714 1104 62-63 1.3986152270089534 1104 64-65 1.2472497011654617 1104 66-67 1.3205796389249969 1104 68-69 1.5985549659455494 1104 70-71 1.545296613846162 1104 72-73 1.2906018667850283 1104 74-75 1.0994747035877452 1104 76-77 1.3276226066759307 1104 78-79 1.5283674985426359 1104 80-81 1.432633373709571 1104 82-83 1.6091665239797308 1104 84-85 1.6769656863662306 1104 86-87 1.694206965931862 1104 88-89 1.6421510383576141 1104 90-91 1.990434994418674 1104 92-93 1.8353163568505373 1104 94-95 1.9107414738369606 1104 96-97 1.673056567954319 1104 98-99 1.5928095942455194 1104 100-101 1.5792549806683986 1104 102-103 1.3843309937088897 1104 104-105 1.8151947586061468 1104 106-107 1.5751676090031843 1104 108-109 1.7113750188110117 1104 110-111 1.6224929829095451 1104 112-113 1.9548321350070665 1104 114-115 1.8730992624292142 1104 116-117 1.8012694276071173 1104 118-119 1.65353328800418 1104 120-121 1.1953915258435508 1104 122-123 1.396571592988213 1104 124-125 1.6557030728871744 1105 1 -0.8958312066478626 1105 2 -0.3245785073655334 1105 3 -0.07279806964654156 1105 4 -0.8676876228851071 1105 5 -0.08455994655497534 1105 6 -0.8874135516411066 1105 7 -0.3107451368766476 1105 8 -0.8939580398917677 1105 9 -0.8814932660537345 1105 10-11 -0.5713339727928144 1105 12-13 -0.5714741352154711 1105 14-15 -0.6752266958370292 1105 16-17 -0.2982086803972521 1105 18-19 -0.05268816410683286 1105 20-21 0.060772631596488935 1105 22-23 0.1810946848610051 1105 24-25 -0.4662672734027886 1105 26-27 -0.14696474688768646 1105 28-29 -0.33311861703128187 1105 30-31 -0.12739167819271557 1105 32-33 -0.02187957966386378 1105 34-35 -0.048657586445251866 1105 36-37 0.12904190806578697 1105 38-39 0.27465668726230774 1105 40-41 -0.11914416036177045 1105 42-43 0.05619389179906875 1105 44-45 -0.002321762551197537 1105 46-47 -0.2904220373965245 1105 48-49 -0.6010484086479799 1105 50-51 -0.3456073855902382 1105 52-53 -0.28506375169324016 1105 54-55 -0.2595073697830692 1105 56-57 -0.36788450218983826 1105 58-59 -0.01082214716127794 1105 60-61 0.2337827114307025 1105 62-63 0.07279590284525739 1105 64-65 -0.06744719134600174 1105 66-67 -0.2797345060911347 1105 68-69 -0.22558750306447095 1105 70-71 -0.08454291748343223 1105 72-73 -0.32276627721225637 1105 74-75 -0.23973823579076026 1105 76-77 -0.20956537090939875 1105 78-79 0.06863324824546879 1105 80-81 0.29462182675762705 1105 82-83 0.07787937845417758 1105 84-85 0.4008789144185272 1105 86-87 0.44093815968292205 1105 88-89 0.3703519213598234 1105 90-91 0.49516839567525395 1105 92-93 0.3944732595504874 1105 94-95 0.2334617998685431 1105 96-97 0.4085167479509195 1105 98-99 0.7667992359503941 1105 100-101 0.36357744628225674 1105 102-103 0.4387757211668628 1105 104-105 0.15731566198532576 1105 106-107 0.2877461452572163 1105 108-109 0.3381792300523081 1105 110-111 0.28119989409990254 1105 112-113 -0.06045059550745435 1105 114-115 -0.43304354619703744 1105 116-117 0.07221950402077226 1105 118-119 -0.1668097243918112 1105 120-121 -0.19132523421758307 1105 122-123 0.05279506046995763 1105 124-125 -0.07249781011503131 1106 1 0.5528867420700863 1106 2 0.9446522618652367 1106 3 0.9207916739431994 1106 4 1.1900046848071995 1106 5 0.4410810790860502 1106 6 0.7215608073332511 1106 7 0.5482292220977136 1106 8 0.6316829857492579 1106 9 0.16337852881806114 1106 10-11 0.2523839759251345 1106 12-13 0.329166890425558 1106 14-15 -0.34509849070882126 1106 16-17 0.11845798626941573 1106 18-19 0.8126964512777803 1106 20-21 1.0062854521093065 1106 22-23 0.9118639156302315 1106 24-25 1.315784008648496 1106 26-27 1.6414967915738536 1106 28-29 1.577137793225127 1106 30-31 1.391839091038051 1106 32-33 1.3338896511053626 1106 34-35 1.374419336631668 1106 36-37 1.4687854978093746 1106 38-39 1.1240156616212822 1106 40-41 1.2718814806638719 1106 42-43 1.2805528661580432 1106 44-45 1.3406269553975179 1106 46-47 1.3473984754239936 1106 48-49 1.34125928365971 1106 50-51 1.6031105631277107 1106 52-53 1.7309618893323986 1106 54-55 1.936005450729752 1106 56-57 2.100064215758877 1106 58-59 2.2103316989925688 1106 60-61 2.060705788353779 1106 62-63 1.8420266720760239 1106 64-65 1.8235784496796406 1106 66-67 1.6625731862165587 1106 68-69 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-3.059158315035127 2216 84-85 -3.034176621443457 2216 86-87 -2.8971807106940126 2216 88-89 -2.9232348681947116 2216 90-91 -3.242528047666351 2216 92-93 -3.509771689049302 2216 94-95 -3.595499561350884 2216 96-97 -3.5156088576262974 2216 98-99 -3.6326306352536726 2216 100-101 -3.994584553008778 2216 102-103 -4.099021159371954 2216 104-105 -3.8014164661016387 2216 106-107 -4.052750723465458 2216 108-109 -3.8221953416125842 2216 110-111 -3.858570281960599 2216 112-113 -3.76622868838232 2216 114-115 -3.7218743390600864 2216 116-117 -3.5814937182408393 2216 118-119 -3.664210173405163 2216 120-121 -3.592291203731939 2216 122-123 -3.6785649773416935 2216 124-125 -3.930984753279393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7813.0 3 1867.0 4 2375.0 5 2111.0 6 1878.0 7 986.0 8 653.0 9 651.0 10 803.0 11 880.0 12 867.0 13 812.0 14 691.0 15 662.0 16 590.0 17 533.0 18 528.0 19 487.0 20 459.0 21 437.0 22 530.0 23 2213.0 24 5237.0 25 1934.0 26 4582.0 27 2537.0 28 1576.0 29 1612.0 30 1461.0 31 1512.0 32 1960.0 33 2610.0 34 3793.0 35 8227.0 36 11067.0 37 19701.0 38 11545.0 39 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.11679722140112 24.39033402305646 9.807308601832693 14.685560153709726 2 12.161600487683227 39.11164066613095 30.507910997810967 18.21884784837485 3 15.234699721057396 49.879001717990874 19.94254890732086 14.943749653630872 4 8.70693876042754 31.3653034264229 26.63445638216302 33.29330143098654 5 8.98484050660976 57.465657881366106 20.74106920156306 12.808432410461066 6 19.875455032614518 48.942106916495746 15.47572851414528 15.706709536744462 7 21.461296877886568 52.933678182154075 13.066691298725292 12.538333641234068 8 21.94934978572484 49.3747229200532 13.898330131520614 14.777597162701344 9 24.499090094500843 36.78419997598219 12.159478259263022 26.557231670253945 10-11 18.956424560366482 47.86971700901434 16.720851189596573 16.45300724102261 12-13 20.523892432100418 47.11106390249985 17.58069208850383 14.7843515768959 14-15 18.88507568164287 45.99651730492981 18.040268084379143 17.07813892904817 16-17 16.48764223437269 49.47862790010345 17.547934830796514 16.485795034727353 18-19 16.7877514259982 47.226289171650926 20.96806225896589 15.017897143384983 20-21 19.8922149051916 44.92754292469821 20.204394528544118 14.97584764156607 22-23 18.926869366041082 46.121804344613565 19.787664400768435 15.163661888576918 24-25 17.59989654726497 45.9260867155604 20.878978773715616 15.595037963459015 26-27 17.087982119107433 45.93061918132112 20.816554603221515 16.164844096349935 28-29 17.063765130767703 46.21090015656101 20.854943217767598 15.870391494903686 30-31 17.23529629082311 46.03590956110536 20.9652541746712 15.763539973400325 32-33 16.751791783655978 46.49216787350377 20.739895817940003 16.01614452490025 34-35 16.681598197133145 46.213702526969115 21.197077730161077 15.907621545736664 36-37 17.090405264980948 46.02286110148944 20.58699919177924 16.299734441750374 38-39 17.41724545588887 45.76021501403872 20.769451012265407 16.053088517807005 40-41 16.87882202967096 46.234781162820774 20.835719696269884 16.050677111238386 42-43 16.658430588333097 46.1365095346077 20.887464380885525 16.317595496173688 44-45 16.783271682760468 46.10493066042311 21.072027264792673 16.039770392023755 46-47 16.70099854974736 46.19886011989987 20.930748127141893 16.169393203210877 48-49 16.475630143439027 46.146172958095114 20.7437031837368 16.634493714729054 50-51 16.901675157147015 46.174203411279485 20.62286102246013 16.30126040911337 52-53 16.766574917333234 46.413463137088286 20.66761494836791 16.15234699721057 54-55 16.58169155883141 46.43982007444558 20.71360617732088 16.264882189402126 56-57 16.759732671941176 46.569028187683884 20.078240105674947 16.592999034699996 58-59 16.53340723804245 46.205686043557584 20.3681378322825 16.892768886117462 60-61 16.231867994846148 46.266457926359 20.571080231092147 16.930593847702703 62-63 16.2148306885149 46.37453584822006 20.527054737581054 16.88357872568399 64-65 15.962482508924488 46.686740032973276 20.2864149183288 17.064362539773438 66-67 15.995973067580424 46.88236000406388 19.879746192424562 17.241920735931135 68-69 16.131594502733858 46.38872469336486 20.344595093837743 17.135085710063542 70-71 15.819978755830602 46.52704013300698 20.201819609292016 17.45116150187041 72-73 16.032307521796955 46.43444288458697 19.9857765627309 17.54747303088518 74-75 15.762322829736434 46.58313666991488 20.278115171639826 17.376425328708855 76-77 15.66284537149086 46.49432348295197 20.604047924768828 17.23878322078834 78-79 15.918536990856195 46.40251223792371 20.377759305440104 17.301191465779993 80-81 15.777271824617955 46.72913597465514 20.165055672807377 17.328536527919532 82-83 15.534630695200008 46.48474660804832 20.147130810651053 17.83349188610062 84-85 16.14036870104921 46.09086375055416 20.43972587557263 17.329041672824 86-87 16.000738893506973 46.23487577352914 20.261383578091806 17.50300175487208 88-89 15.983706871469938 45.80733652607224 20.53312889952109 17.675827702936736 90-91 16.058117731819735 45.88871544294911 20.190646850724626 17.862519974506526 92-93 15.857246447095555 45.84620784895133 20.462050777551465 17.834494926401646 94-95 15.961652135362788 45.87474139204965 20.39077508497118 17.77283138761637 96-97 15.812028964090437 46.174911334417025 20.24807891236885 17.764980789123687 98-99 15.494739953264554 46.365533994033385 19.95354164165181 18.186184411050256 100-101 15.463831830944288 46.612235850450716 20.467895670164033 17.456036648440964 102-103 15.212612679178367 47.057410964977095 20.267936308556227 17.462040047288312 104-105 14.663051861978504 47.55561918925535 20.472757335451718 17.308571613314427 106-107 14.65152578690705 48.11724176148958 20.025953155016996 17.205279296586376 108-109 14.772055563765333 48.434960100487665 19.580316240579283 17.21266809516773 110-111 14.351817644451012 48.87874981528004 19.986238362642233 16.78319417762672 112-113 14.500992934004525 49.19641620098831 19.45319355285642 16.849397312150742 114-115 14.257610462538791 49.46708290232008 19.496268656716417 16.779037978424707 116-117 14.334731047731639 49.64118146889316 19.438081867888283 16.586005615486922 118-119 14.242895870760961 49.98129580795182 19.276863607184257 16.49894471410296 120-121 14.080077583818232 49.67211600628059 19.457375080816476 16.790431329084697 122-123 14.206812472292004 49.531273089995565 19.35865228313876 16.903262154573667 124-125 14.363427958698846 49.78646897329476 18.81568852201403 17.034414545992366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5626.0 1 5647.0 2 6520.5 3 5596.0 4 2842.5 5 1395.5 6 787.0 7 680.0 8 851.5 9 881.0 10 550.0 11 300.5 12 260.5 13 235.5 14 214.0 15 192.5 16 174.0 17 182.0 18 215.5 19 223.5 20 224.5 21 255.0 22 274.5 23 316.5 24 399.5 25 521.5 26 647.0 27 756.0 28 913.5 29 1092.5 30 1265.5 31 1408.0 32 1469.0 33 1528.5 34 1629.5 35 1701.0 36 1829.5 37 1953.5 38 1994.5 39 2063.0 40 2086.5 41 1997.0 42 1999.5 43 2181.0 44 2329.0 45 2294.5 46 2292.5 47 2491.5 48 2707.0 49 2634.5 50 2555.5 51 2483.0 52 2320.5 53 2405.5 54 2448.5 55 2345.0 56 2236.5 57 2078.5 58 1957.0 59 2014.0 60 2048.0 61 2000.5 62 1684.0 63 1029.0 64 570.5 65 409.5 66 338.5 67 270.5 68 201.5 69 160.5 70 134.0 71 124.0 72 129.5 73 148.0 74 125.0 75 67.0 76 41.0 77 41.5 78 35.5 79 19.5 80 10.5 81 4.5 82 3.0 83 2.0 84 1.5 85 2.0 86 1.5 87 0.5 88 0.5 89 0.0 90 0.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014777597162701344 2 0.00461799911334417 3 0.005541598936013004 4 0.023089995566720853 5 0.021242795921383183 6 0.035096793261415696 7 0.011083197872026008 8 0.0 9 0.017548396630707848 10-11 0.0 12-13 0.005079799024678587 14-15 0.020780996010048767 16-17 0.0 18-19 0.013392197428698094 20-21 9.23599822668834E-4 22-23 0.0 24-25 0.00923599822668834 26-27 0.0 28-29 0.006926998670016255 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.008774198315353924 38-39 0.0 40-41 0.016624796808039015 42-43 0.00785059849268509 44-45 4.61799911334417E-4 46-47 0.01385399734003251 48-49 0.002770799468006502 50-51 0.01246859760602926 52-53 0.005541598936013004 54-55 0.00461799911334417 56-57 0.014315797251366927 58-59 0.022628195655386436 60-61 0.003232599379340919 62-63 0.00923599822668834 64-65 0.002308999556672085 66-67 9.23599822668834E-4 68-69 0.0 70-71 0.006465198758681838 72-73 0.0 74-75 0.006926998670016255 76-77 0.017548396630707848 78-79 0.001847199645337668 80-81 0.005079799024678587 82-83 9.23599822668834E-4 84-85 0.0 86-87 0.001847199645337668 88-89 0.005079799024678587 90-91 0.008312398404019507 92-93 0.014315797251366927 94-95 0.0 96-97 0.0 98-99 0.002770799468006502 100-101 0.0 102-103 0.0 104-105 0.01246859760602926 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.006465198758681838 114-115 0.0 116-117 0.0 118-119 0.006926998670016255 120-121 0.001847199645337668 122-123 0.0 124-125 0.0840475838628639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 108272.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.368368553273235 #Duplication Level Percentage of deduplicated Percentage of total 1 81.0013325718637 43.229089700014775 2 10.667497360815464 11.386138613861387 3 3.653323641901597 5.849157676961727 4 1.7340740356160116 3.701788089256687 5 0.8739594690479899 2.332089552238806 6 0.46034300745894124 1.474065316979459 7 0.351314400429192 1.3124353480124131 8 0.21978782686949447 0.9383774198315354 9 0.14364086322966962 0.689929067533619 >10 0.7770451516882128 7.248411408305009 >50 0.06057144834986068 2.275749963056007 >100 0.044995933059896506 4.837815871139353 >500 0.005191838429988059 2.1695359834490913 >1k 0.005191838429988059 6.594502733855475 >5k 0.0017306128099960196 5.960913255504655 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6454 5.960913255504655 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2960 2.7338554750997486 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2876 2.6562730899955667 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1304 1.2043741687601595 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 994 0.9180582237328211 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 788 0.7277966602630412 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.5236810994532288 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 420 0.3879119255209103 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 398 0.36759272942219595 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 337 0.31125314023939704 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 309 0.2853923452046697 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 292 0.2696911482192995 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 281 0.2595315501699424 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 266 0.24567755282990986 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 265 0.24475395300724104 No Hit GAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 257 0.23736515442589037 No Hit GAACAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 218 0.20134476134180584 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 210 0.19395596276045515 No Hit GAACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 169 0.15608837003103296 No Hit GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 159 0.1468523718043446 No Hit AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 156 0.14408157233633812 No Hit GAACAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 152 0.14038717304566278 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 147 0.1357691739323186 No Hit GAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.12838037535096794 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 128 0.11822077730161075 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.11822077730161075 No Hit GAACAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 127 0.11729717747894193 No Hit GAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 123 0.11360277818826658 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.11267917836559777 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.1062139796069159 No Hit TAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.10067238067090292 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.235998226688341E-4 0.0 18-19 0.0 0.0 0.0 9.235998226688341E-4 0.0 20-21 0.0 0.0 0.0 0.002770799468006502 0.0 22-23 0.0 0.0 0.0 0.01062139796069159 0.0 24-25 0.0 0.0 0.0 0.018010196542042264 0.0 26-27 0.0 0.0 0.0 0.022166395744052016 0.0 28-29 0.0 0.0 0.0 0.028169794591399438 0.0 30-31 0.0 0.0 0.0 0.05356878971479238 0.0 32-33 0.0 0.0 0.0 0.09051278262154575 0.0 34-35 0.0 0.0 0.0 0.11775897739027634 0.0 36-37 0.0 0.0 0.0 0.14592877198167578 0.0 38-39 0.0 0.0 0.0 0.20550096054381556 0.0 40-41 0.0 0.0 0.0 0.29093394414068274 0.0 42-43 0.0 0.0 0.0 0.36851632924486477 0.0 44-45 0.0 0.0 0.0 0.4479459139943845 0.0 46-47 0.0 0.0 0.0 0.5476946948426187 0.0 48-49 0.0 0.0 0.0 0.6451344761341806 0.0 50-51 0.0 0.0 0.0 0.7578136544997783 0.0 52-53 0.0 0.0 0.0 0.8617186345500222 0.0 54-55 0.0 0.0 0.0 0.9670090143342692 0.0 56-57 0.0 0.0 0.0 1.1110905866706073 0.0 58-59 0.0 0.0 0.0 1.252401359538939 0.0 60-61 0.0 0.0 0.0 1.4325033249593617 0.0 62-63 0.0 0.0 0.0 1.619994088961135 0.0 64-65 0.0 0.0 0.0 1.7968634550022164 0.0 66-67 0.0 0.0 0.0 1.9885104182059998 0.0 68-69 0.0 0.0 0.0 2.2110979754691886 0.0 70-71 0.0 0.0 0.0 2.4392271316683907 0.0 72-73 0.0 0.0 0.0 2.696449682281661 0.0 74-75 0.0 0.0 0.0 2.9278114378602043 0.0 76-77 0.0 0.0 0.0 3.18457218856214 0.0 78-79 0.0 0.0 0.0 3.4570341362494457 0.0 80-81 0.0 0.0 0.0 3.7415028816314466 0.0 82-83 0.0 0.0 0.0 4.085082015664254 0.0 84-85 0.0 0.0 0.0 4.419425151470371 0.0 86-87 0.0 0.0 0.0 4.750997487808482 0.0 88-89 0.0 0.0 0.0 5.131058814836708 0.0 90-91 0.0 0.0 0.0 5.538366336633663 0.0 92-93 0.0 0.0 0.0 5.965531254617999 0.0 94-95 0.0 0.0 0.0 6.425945766218413 0.0 96-97 0.0 0.0 0.0 6.849878084823407 0.0 98-99 0.0 0.0 0.0 7.336615191369884 0.0 100-101 0.0 0.0 0.0 7.8838480863011675 0.0 102-103 0.0 0.0 0.0 8.471719373429881 0.0 104-105 0.0 0.0 0.0 9.042965863750554 0.0 106-107 0.0 0.0 0.0 9.601281956553866 0.0 108-109 0.0 0.0 0.0 10.17991724545589 0.0 110-111 0.0 0.0 0.0 10.829669720703414 0.0 112-113 0.0 0.0 0.0 11.47110979754692 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 50 9.094947E-12 95.25437 1 GAACAAA 670 0.0 93.29952 1 GGTATCA 240 0.0 76.898056 1 ATGGGCG 25 0.0020546794 71.37479 7 CACGGGA 25 0.0020546794 71.37479 9 GTATCAA 630 0.0 67.09385 1 GAAAACA 410 0.0 62.438072 1 ACGCAAA 90 2.3646862E-11 59.533978 1 CTAAGAC 30 0.004216653 59.533978 3 CGCAAAA 60 3.114037E-7 59.533978 2 CGAAAGG 15 0.00423784 59.478985 42-43 CGATCCA 15 0.00423784 59.478985 72-73 TACCTCC 90 2.3646862E-11 59.47898 7 CACTACC 100 6.730261E-11 53.58058 4 TAATATC 35 0.007759749 51.029125 2 ACTACCT 105 1.09139364E-10 51.029125 5 GTCTTAG 35 0.007759749 51.029125 1 GGGATTC 70 9.0859794E-7 50.981987 7 ATCAACG 805 0.0 50.289574 3 TCAACGC 805 0.0 49.550022 4 >>END_MODULE