##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940145_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 108272 Sequences flagged as poor quality 0 Sequence length 125 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51226540564504 33.0 31.0 34.0 31.0 34.0 2 32.46721220629526 33.0 31.0 34.0 31.0 34.0 3 32.53129156199202 34.0 31.0 34.0 30.0 34.0 4 36.086716787350376 37.0 35.0 37.0 35.0 37.0 5 36.13962982119107 37.0 35.0 37.0 35.0 37.0 6 36.29047214422935 37.0 37.0 37.0 35.0 37.0 7 36.268361164474655 37.0 37.0 37.0 35.0 37.0 8 36.27874242648146 37.0 37.0 37.0 35.0 37.0 9 38.011655829762084 39.0 39.0 39.0 35.0 39.0 10-11 38.03927146445988 39.0 39.0 39.0 35.0 39.0 12-13 38.012274641643266 39.0 39.0 39.0 35.0 39.0 14-15 39.08532676961726 40.5 38.5 41.0 36.0 41.0 16-17 39.12677792965864 40.0 38.5 41.0 36.0 41.0 18-19 39.4280192478203 41.0 39.0 41.0 36.0 41.0 20-21 39.35552127973992 41.0 39.0 41.0 35.5 41.0 22-23 39.0813968523718 41.0 38.5 41.0 35.0 41.0 24-25 38.946218782326 41.0 38.0 41.0 35.0 41.0 26-27 38.672537682872765 41.0 37.0 41.0 35.0 41.0 28-29 38.399955667208516 40.5 36.5 41.0 35.0 41.0 30-31 37.62750295551943 39.0 35.0 41.0 34.5 41.0 32-33 36.67186345500221 37.0 35.0 41.0 33.5 41.0 34-35 36.12183667060736 36.0 35.0 41.0 33.0 41.0 36-37 35.84149641643269 36.0 35.0 41.0 33.0 41.0 38-39 35.66709767991725 35.0 35.0 41.0 32.0 41.0 40-41 35.44819528594651 35.0 35.0 41.0 31.0 41.0 42-43 35.04210229791636 35.0 35.0 41.0 30.5 41.0 44-45 34.54529795330279 35.0 35.0 41.0 25.0 41.0 46-47 33.37856047731639 35.0 34.0 41.0 16.0 41.0 48-49 32.4122026008571 35.0 33.0 41.0 6.0 41.0 50-51 32.31360370178809 35.0 33.0 41.0 2.0 41.0 52-53 32.41983153539235 35.0 33.5 41.0 2.0 41.0 54-55 32.35029832274272 35.0 34.0 40.0 2.0 41.0 56-57 32.227016218412885 35.0 34.0 40.0 2.0 41.0 58-59 32.0523265479533 35.0 34.0 40.0 2.0 41.0 60-61 31.863773644155458 35.0 33.5 40.0 2.0 41.0 62-63 31.652084564799765 35.0 33.0 39.0 2.0 41.0 64-65 31.425382370326584 35.0 33.0 39.0 2.0 41.0 66-67 31.18625775823851 35.0 33.0 38.5 2.0 41.0 68-69 30.91790582976208 35.0 33.0 37.0 2.0 41.0 70-71 30.688968523718046 35.0 33.0 37.0 2.0 40.5 72-73 30.50604496083937 35.0 33.0 36.5 2.0 40.0 74-75 30.268846054381555 35.0 33.0 36.0 2.0 39.0 76-77 30.016246120880744 35.0 33.0 36.0 2.0 39.0 78-79 29.747783360425593 35.0 33.0 35.0 2.0 38.0 80-81 29.496430286685385 35.0 33.0 35.0 2.0 37.0 82-83 29.271385953893898 35.0 32.5 35.0 2.0 37.0 84-85 29.07982211467415 35.0 32.0 35.0 2.0 36.5 86-87 28.863418058223733 35.0 32.0 35.0 2.0 36.0 88-89 28.662221072853555 35.0 31.0 35.0 2.0 36.0 90-91 28.493239249298064 35.0 31.0 35.0 2.0 36.0 92-93 28.30222956997192 35.0 31.0 35.0 2.0 35.0 94-95 28.154379710359095 35.0 31.0 35.0 2.0 35.0 96-97 28.006031106842027 35.0 30.0 35.0 2.0 35.0 98-99 27.8613307226245 35.0 30.0 35.0 2.0 35.0 100-101 27.717498522240284 35.0 29.0 35.0 2.0 35.0 102-103 26.899660115265256 33.5 26.5 35.0 2.0 35.0 104-105 27.3663135436678 35.0 29.0 35.0 2.0 35.0 106-107 27.30679677109502 35.0 29.0 35.0 2.0 35.0 108-109 27.174920570415253 35.0 28.0 35.0 2.0 35.0 110-111 27.0023875055416 35.0 27.0 35.0 2.0 35.0 112-113 26.817131853110684 35.0 26.0 35.0 2.0 35.0 114-115 26.59910687897148 35.0 24.5 35.0 2.0 35.0 116-117 26.391721774789417 35.0 23.5 35.0 2.0 35.0 118-119 26.173867666617408 35.0 19.5 35.0 2.0 35.0 120-121 25.931884513078174 35.0 8.5 35.0 2.0 35.0 122-123 25.680078875424854 35.0 2.0 35.0 2.0 35.0 124-125 24.50364360130043 33.0 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05188699936326202 1101 2 -0.04593681140323724 1101 3 -0.05098159358355758 1101 4 -0.3916376740006058 1101 5 -0.017795299056615477 1101 6 0.014537232526684818 1101 7 0.16875048783553126 1101 8 -0.033110458089481654 1101 9 -8.795044088287796E-4 1101 10-11 0.014701226955352809 1101 12-13 -0.21824772103704504 1101 14-15 -0.4691157727057771 1101 16-17 -0.2039479782721898 1101 18-19 -0.18252236580220682 1101 20-21 -0.05132230894565737 1101 22-23 -0.2703715100003592 1101 24-25 -0.046547399259232236 1101 26-27 -0.05514918110045386 1101 28-29 -0.06681156734986615 1101 30-31 -0.06084156971608934 1101 32-33 -0.006745177189728224 1101 34-35 -0.2716203156090842 1101 36-37 -0.1143612732045014 1101 38-39 0.10029969746888412 1101 40-41 0.434151280895108 1101 42-43 -4.056988632541447E-4 1101 44-45 -0.24709605871644413 1101 46-47 -0.6393636231587791 1101 48-49 -0.07100871472812997 1101 50-51 -0.5682237058538533 1101 52-53 -0.9944300083129498 1101 54-55 -0.7344361096958991 1101 56-57 -0.7063723409349372 1101 58-59 -0.5969104894380806 1101 60-61 -0.3805633305932936 1101 62-63 -0.6695198018493507 1101 64-65 -0.1642853509773552 1101 66-67 -0.2703211215857344 1101 68-69 -0.7065298806247711 1101 70-71 -0.669656496289825 1101 72-73 -1.190667027323503 1101 74-75 -0.7197243261171344 1101 76-77 -1.1044615549657166 1101 78-79 -1.3073495564011175 1101 80-81 -1.3561187029427373 1101 82-83 -1.2448335016536376 1101 84-85 -1.23918285739758 1101 86-87 -1.0862973772371554 1101 88-89 -1.5305176557420985 1101 90-91 -1.7018202365647426 1101 92-93 -1.597135434859517 1101 94-95 -1.925147266303938 1101 96-97 -1.9355217311782056 1101 98-99 -1.9136187304482206 1101 100-101 -1.5834800433905514 1101 102-103 -1.767336128004704 1101 104-105 -1.7589071987024418 1101 106-107 -1.5014154266036854 1101 108-109 -1.5288124586289378 1101 110-111 -1.3259662910120937 1101 112-113 -1.6519748141004236 1101 114-115 -1.6487765750385996 1101 116-117 -1.6970299523533967 1101 118-119 -1.5309145123192387 1101 120-121 -2.217835845882128 1101 122-123 -2.595310708695173 1101 124-125 -2.551642451991384 1102 1 0.18584293806058128 1102 2 0.11646740315615034 1102 3 0.014679325956677758 1102 4 0.10697343711051133 1102 5 -0.16348495422902687 1102 6 -0.2772731123009038 1102 7 -0.42052154281580556 1102 8 0.09615199401780217 1102 9 -0.01476839329771451 1102 10-11 -0.09605068875345779 1102 12-13 -0.20304178233972436 1102 14-15 -0.156136845502715 1102 16-17 0.04851849682359699 1102 18-19 0.2192736112092888 1102 20-21 0.06711638837235512 1102 22-23 0.2860461145207154 1102 24-25 0.27119685361433454 1102 26-27 0.009505991313339734 1102 28-29 -0.017649689955220538 1102 30-31 0.32471877511149216 1102 32-33 0.0676561638064328 1102 34-35 -0.043339664268088995 1102 36-37 0.3047718685579568 1102 38-39 0.6500602338673502 1102 40-41 -0.03383243557998128 1102 42-43 -0.4663060820050191 1102 44-45 -0.632057744540198 1102 46-47 -0.25806573043847436 1102 48-49 0.29965699408413116 1102 50-51 -0.1660924798002128 1102 52-53 -0.42108230716352324 1102 54-55 -0.631562546477511 1102 56-57 -0.7648972451494984 1102 58-59 -0.5581413323499582 1102 60-61 -0.8662434072216456 1102 62-63 -1.3520389589374737 1102 64-65 -1.2797786459965117 1102 66-67 -1.4244877882523994 1102 68-69 -1.5626362024638496 1102 70-71 -1.7451593698530417 1102 72-73 -1.7406191346031967 1102 74-75 -1.474609383588401 1102 76-77 -1.2308025511342962 1102 78-79 -1.4684846138723842 1102 80-81 -1.077263338958062 1102 82-83 -1.1272329269409909 1102 84-85 -1.3547958842175056 1102 86-87 -1.1255454615283433 1102 88-89 -1.0653356634049338 1102 90-91 -0.954885370664357 1102 92-93 -1.0323844770051096 1102 94-95 -0.9227286839284616 1102 96-97 -0.7697650261973621 1102 98-99 -0.6436235197202507 1102 100-101 -0.9908794686779032 1102 102-103 -0.7263399594223294 1102 104-105 -0.497101643146884 1102 106-107 -0.6893464610864442 1102 108-109 -0.3085298915791306 1102 110-111 -0.5160046051883427 1102 112-113 -0.32819607846824184 1102 114-115 -0.6370907512838109 1102 116-117 -0.46295428185531406 1102 118-119 -0.5759097230472072 1102 120-121 -0.449181631322741 1102 122-123 -0.4635817814921097 1102 124-125 -0.6258282757461728 1103 1 0.16216477714104371 1103 2 0.16267429970787362 1103 3 0.05651840641644412 1103 4 0.08030677044384049 1103 5 0.21053803427671625 1103 6 0.2753705658600154 1103 7 0.19097271005775696 1103 8 0.24994509746607463 1103 9 0.10523160670228293 1103 10-11 0.2523401158442411 1103 12-13 0.1332800567407375 1103 14-15 0.07282867173866236 1103 16-17 0.3803575772833625 1103 18-19 0.23789430086445407 1103 20-21 0.22562213549879573 1103 22-23 -0.053149287778133214 1103 24-25 -0.0036307325925619693 1103 26-27 -0.07348251443378473 1103 28-29 0.05166065487235727 1103 30-31 -0.21792490304942191 1103 32-33 -0.551884066078614 1103 34-35 -0.7809258711646407 1103 36-37 -0.5133890509822763 1103 38-39 -0.3277558580866682 1103 40-41 -0.3901542746604463 1103 42-43 -0.4926279210854787 1103 44-45 -0.3798738364942196 1103 46-47 -0.8945025120476657 1103 48-49 -1.3278142702836853 1103 50-51 -0.9758625947427433 1103 52-53 -1.1590133416462827 1103 54-55 -1.097769443029236 1103 56-57 -0.6158167853793799 1103 58-59 -0.5120493783269673 1103 60-61 -0.49417444170440206 1103 62-63 -0.564797579627129 1103 64-65 -0.3441464620884638 1103 66-67 0.07884554508093089 1103 68-69 -0.14194654729143608 1103 70-71 -0.037573162956491046 1103 72-73 0.05294408378760451 1103 74-75 -0.138057659450471 1103 76-77 0.016094000589841784 1103 78-79 0.3714004435988798 1103 80-81 0.07457574150171098 1103 82-83 0.0939164983463634 1103 84-85 0.1835949203801981 1103 86-87 0.1293970672072895 1103 88-89 0.4375378998134529 1103 90-91 0.5370686523241481 1103 92-93 0.5450867873627061 1103 94-95 0.585547178140505 1103 96-97 0.6892004910440157 1103 98-99 0.8423534917740021 1103 100-101 0.20693662327612117 1103 102-103 0.39377498310640746 1103 104-105 0.2062316901864456 1103 106-107 0.39858457339631315 1103 108-109 -0.20450690307338348 1103 110-111 0.045144820099018546 1103 112-113 0.21594185256624243 1103 114-115 0.2023345360725095 1103 116-117 0.060608936535491154 1103 118-119 0.19144659879187031 1103 120-121 -0.13630806810435203 1103 122-123 0.045383735749268794 1103 124-125 0.24474643689750764 1104 1 0.09801466676576354 1104 2 0.08545575666151706 1104 3 0.12548087882262848 1104 4 0.04604628479263084 1104 5 -0.06332510038112105 1104 6 0.25832862325516004 1104 7 -0.2823164731652028 1104 8 0.10729609084356184 1104 9 0.17728458683473747 1104 10-11 0.366269475667643 1104 12-13 0.25482531060387004 1104 14-15 0.4922988704141602 1104 16-17 0.48647236757033596 1104 18-19 0.4388214531823422 1104 20-21 0.27129542468200896 1104 22-23 0.24130987336977228 1104 24-25 0.3409388038312855 1104 26-27 0.12779783876709416 1104 28-29 0.0947732376538184 1104 30-31 -0.10492269554390532 1104 32-33 -0.0794778850631701 1104 34-35 0.25291518843800986 1104 36-37 -0.25727426069530424 1104 38-39 -0.25727020687254054 1104 40-41 0.3116117297545671 1104 42-43 0.25529702372688945 1104 44-45 0.22816148359408572 1104 46-47 0.06936062261016929 1104 48-49 0.6920753544403766 1104 50-51 0.6977577143080325 1104 52-53 0.40588732060537325 1104 54-55 0.5994491000171251 1104 56-57 0.5558609188148793 1104 58-59 0.782895434035062 1104 60-61 0.6461566841234117 1104 62-63 0.88838122831989 1104 64-65 0.6885246195892343 1104 66-67 0.5642539336019041 1104 68-69 0.43951040635094785 1104 70-71 0.07116855889781704 1104 72-73 0.09938999990239594 1104 74-75 0.5404853869071466 1104 76-77 0.6456304244308981 1104 78-79 0.5201200903979988 1104 80-81 0.22029318079530924 1104 82-83 0.5522829442624797 1104 84-85 0.3181203066936611 1104 86-87 0.4478959634545312 1104 88-89 0.48378514043155363 1104 90-91 0.6269141269378338 1104 92-93 0.6626143811816902 1104 94-95 0.7599842642332213 1104 96-97 0.39390247779898147 1104 98-99 0.6462828515974053 1104 100-101 0.896583422393114 1104 102-103 0.6406844753801373 1104 104-105 0.6068718667868929 1104 106-107 0.536664043594989 1104 108-109 0.8525571145866628 1104 110-111 0.8602000077369851 1104 112-113 1.2396504618377655 1104 114-115 1.3238135647700808 1104 116-117 1.3697700844383611 1104 118-119 1.6070315877543457 1104 120-121 1.8462967884077912 1104 122-123 1.8265757887294036 1104 124-125 1.724525686345629 1105 1 -0.09501471003844131 1105 2 -0.22168467465109387 1105 3 -0.03014826025022188 1105 4 0.3364606165976909 1105 5 0.0046405983792823235 1105 6 0.15075518124463372 1105 7 0.09610091518595709 1105 8 -0.03364464612366902 1105 9 0.08779570926638769 1105 10-11 0.022981141485267642 1105 12-13 0.19174159520227363 1105 14-15 0.16603379994378997 1105 16-17 0.14728065420643333 1105 18-19 0.3503301983003553 1105 20-21 0.3274170072936613 1105 22-23 0.32044045581160674 1105 24-25 0.28918978022795017 1105 26-27 0.531389280438006 1105 28-29 0.3789683471800558 1105 30-31 0.09861355848903486 1105 32-33 0.45132106212651024 1105 34-35 0.4124074621686873 1105 36-37 0.34263659004336233 1105 38-39 0.6264749111440935 1105 40-41 0.3938200843139157 1105 42-43 0.3094233609658019 1105 44-45 0.5071774455570619 1105 46-47 -0.3543743069194605 1105 48-49 0.08577547330605739 1105 50-51 0.24721432833418078 1105 52-53 -0.18593641856936216 1105 54-55 -0.026102776362563418 1105 56-57 -0.06107319563579239 1105 58-59 -0.0939724552500465 1105 60-61 -0.23366162119158318 1105 62-63 -0.4681309129604614 1105 64-65 -0.3178644108064148 1105 66-67 -0.38089804466265775 1105 68-69 -0.8772029575478442 1105 70-71 -0.9605218809052083 1105 72-73 -0.8247482239047024 1105 74-75 -0.7069038132966234 1105 76-77 -0.6931367686409295 1105 78-79 -0.7007790435806065 1105 80-81 -0.793885796959831 1105 82-83 -0.9203142708844041 1105 84-85 -0.7338409770557028 1105 86-87 -0.992013189202968 1105 88-89 -0.6272056899301361 1105 90-91 -0.5501108348553387 1105 92-93 -0.3813234690475511 1105 94-95 -0.2315041039107797 1105 96-97 -0.37836361152008635 1105 98-99 -0.4566208672003569 1105 100-101 -0.6051146587751646 1105 102-103 -0.1818660425346188 1105 104-105 -0.2233836944289358 1105 106-107 -0.2546205548088132 1105 108-109 -0.3634812620477419 1105 110-111 -0.4071628722086764 1105 112-113 -0.4059812243568359 1105 114-115 -0.3357423870044123 1105 116-117 -0.038109012182456325 1105 118-119 0.17875429109956187 1105 120-121 0.19535859856231852 1105 122-123 0.3611529665184996 1105 124-125 0.30038746253853077 1106 1 0.2447288797051428 1106 2 0.04113583816941713 1106 3 0.2775440474420847 1106 4 0.20825548839255958 1106 5 0.06874316248184442 1106 6 0.016139796629246916 1106 7 0.3076393767244241 1106 8 -0.05287541535443552 1106 9 0.2480521195227965 1106 10-11 -0.15650603800191476 1106 12-13 0.17251082597150003 1106 14-15 -0.07435081544082323 1106 16-17 0.022280654206433326 1106 18-19 0.08750968547984428 1106 20-21 0.3338272637039239 1106 22-23 0.08967122504238034 1106 24-25 0.15136926740743917 1106 26-27 0.24933799838672854 1106 28-29 0.11614783435954479 1106 30-31 -0.05202746715198714 1106 32-33 -0.10957637377092055 1106 34-35 0.5213818211430521 1106 36-37 0.6471237695305447 1106 38-39 0.4053210649902539 1106 40-41 0.4418970073908355 1106 42-43 0.6651925917350354 1106 44-45 -0.2492328108531936 1106 46-47 -0.8864255889707451 1106 48-49 -1.2667886292580448 1106 50-51 -1.9418882357683813 1106 52-53 -1.4679877006206397 1106 54-55 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-0.4392939471221453 2216 84-85 -0.8486938661021384 2216 86-87 -0.8394815810261953 2216 88-89 -0.7196817622449174 2216 90-91 -0.5368176764008901 2216 92-93 -0.8303663616388306 2216 94-95 -0.6297523610300004 2216 96-97 -0.9330575734721123 2216 98-99 -0.4785988891783788 2216 100-101 -0.7877331155871694 2216 102-103 -0.961140531486528 2216 104-105 -0.9261339012114007 2216 106-107 -0.5878209565576 2216 108-109 -0.898900144240816 2216 110-111 -0.9990333673049783 2216 112-113 -2.093459069092745 2216 114-115 -1.9842860476448472 2216 116-117 -2.4726475918823283 2216 118-119 -2.700381358197376 2216 120-121 -2.9588272693332307 2216 122-123 -3.2526807803797624 2216 124-125 -3.3201076337630155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 12.0 5 37.0 6 90.0 7 134.0 8 256.0 9 409.0 10 843.0 11 1017.0 12 1155.0 13 1732.0 14 3211.0 15 3223.0 16 1172.0 17 857.0 18 804.0 19 745.0 20 681.0 21 725.0 22 694.0 23 762.0 24 843.0 25 1009.0 26 1157.0 27 1287.0 28 1470.0 29 1623.0 30 2016.0 31 2295.0 32 2891.0 33 3495.0 34 5925.0 35 20754.0 36 11075.0 37 21163.0 38 12564.0 39 140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.48657687120079 17.034347689522015 14.486447536167619 12.992627903109582 2 16.29260182876143 23.54484159970444 42.62399556663895 17.538561004895172 3 17.550243830353185 37.447354810107875 31.519691148219298 13.48271021131964 4 9.091749480009243 20.286572683152297 36.07118095678299 34.55049688005546 5 7.547657750849712 39.86164474656421 33.622727944436235 18.967969558149843 6 20.157609800262374 40.421463018052144 19.287337632342343 20.13358954934314 7 18.097014925373134 42.21867149401508 23.651544258903503 16.03276932170829 8 25.810920644303238 30.041931431949166 21.86253140239397 22.284616521353627 9 28.81169646815428 12.948869513817055 23.42249150288163 34.81694251514703 10-11 22.16270134476134 28.81539086744495 26.13140978276932 22.89049800502438 12-13 28.69439929067534 28.81816166691296 29.61799911334417 12.869439929067534 14-15 23.647388059701495 24.519266292300873 37.451972809221225 14.381372838776416 16-17 13.752401359538938 28.73365228313876 36.81792153095906 20.69602482636323 18-19 13.231952859465052 18.866373577656272 52.01898921235407 15.882684350524604 20-21 15.734446578986258 16.89633515590365 54.60322151618146 12.765996748928623 22-23 14.694473178661148 17.890128565095313 54.980050243830355 12.435348012413181 24-25 13.830971215878602 17.796465367155715 55.62867421251207 12.743889204453618 26-27 13.582458992167872 17.68555120437417 55.685218708438015 13.046771095019949 28-29 13.390473903445981 17.69448882895697 55.50793841379501 13.407098853802033 30-31 13.667852029741837 17.5966378792777 55.621391954925414 13.11411813605505 32-33 13.458234816018916 17.819934978572487 55.26821338850303 13.453616816905573 34-35 13.471627013447613 17.807466380966456 55.199867001625535 13.521039603960396 36-37 13.950513521501403 17.493442441259052 54.276728978868036 14.279315058371509 38-39 14.18326067681395 17.372450864489434 54.267954780552685 14.176333678143935 40-41 13.980068715826807 17.786685384956407 53.71056228757204 14.522683611644746 42-43 14.061807300132998 17.851337372543224 53.24783877641496 14.839016550908823 44-45 14.285054541088234 18.174051188266045 52.42132875206666 15.119565518579067 46-47 15.029763966435297 18.387157852930446 50.59273934708618 15.990338833548076 48-49 15.182595684941628 19.07510713757943 48.79054603221516 16.95175114526378 50-51 15.536764909623251 19.407217208988722 47.80731326603183 17.248704615356196 52-53 15.382555046549431 20.18758312398404 47.261526525786905 17.168335303679623 54-55 15.20358731140985 20.273758780092454 47.02666007822999 17.495993830267707 56-57 15.492463425447022 20.13216713462391 46.63994384513078 17.735425594798286 58-59 15.550157478133167 19.57254615818009 47.1464592819736 17.73083708171314 60-61 15.967729273169763 19.56470539338607 47.336556711600004 17.13100862184416 62-63 16.448149259934883 19.499388089685272 47.585840626226705 16.46662202415314 64-65 16.146432249453905 19.64791236601597 47.82323575185765 16.382419632672477 66-67 16.18562509235998 19.8153723954485 47.390368701049205 16.608633811142308 68-69 16.708691234875776 19.609310058187866 47.545488131523044 16.13651057541332 70-71 16.206855147826246 19.955850705188006 47.21296031181594 16.624333835169807 72-73 16.260650703119587 19.92564712402152 46.69822430553952 17.11547786731937 74-75 15.766772572779667 20.357063691443773 47.01261637357766 16.863547362198904 76-77 15.277226168155241 20.53311658923607 47.06379480737792 17.12586243523077 78-79 15.284935808626582 20.216126350789693 47.165419783873645 17.333518056710076 80-81 15.177054086005615 20.365376089847793 46.83159080833457 17.625979015812028 82-83 15.156623056354746 20.11323595287685 46.433667838126155 18.29647315264225 84-85 15.337760455149994 19.7294776119403 46.51479606915915 18.417965863750556 86-87 15.3728213985536 19.907360370927968 45.98315307243994 18.736665158078488 88-89 15.225392896232906 19.686144837045624 46.21789750292103 18.870564763800438 90-91 15.500424871614882 19.762995529611704 46.051003066464695 18.685576532308716 92-93 15.245251301187349 19.86717959849076 45.894621143197575 18.99294795712432 94-95 15.166461159062885 20.105109057574456 45.78155234440304 18.94687743895962 96-97 15.406000766605555 20.238386618576794 45.6947709188645 18.660841695953152 98-99 15.414278654985614 20.162284732581178 45.28963252714367 19.13380408528954 100-101 15.572540235989562 20.00554182926548 45.63881127762255 18.783106657122406 102-103 15.75452223828282 19.87014006585358 45.48976868121972 18.885569014643877 104-105 15.61828937706947 19.877528966800746 45.54841808248784 18.955763573641942 106-107 15.848049357174524 19.9991687601596 45.003324959361606 19.14945692330427 108-109 16.00094212837885 19.92001145332539 44.572370699807415 19.506675718488346 110-111 15.57402789322989 20.1861088020689 44.77325205504757 19.466611249653642 112-113 15.579929341676864 20.256771422633754 44.21086660355139 19.95243263213799 114-115 15.177977685828283 20.230530515738142 44.24227870548249 20.349213092951086 116-117 15.108707699128122 20.459583271760014 44.42884586966159 20.002863159450275 118-119 15.125402345030778 20.469837399500328 44.3399232485003 20.06483700696859 120-121 14.935208874029057 20.63849045450767 43.81875109678492 20.60754957467835 122-123 15.316268050854113 20.772509594026072 42.95537565634221 20.9558466987776 124-125 15.216447216447214 20.598290598290596 42.65650265650265 21.52875952875953 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1973.0 1 1331.5 2 2708.5 3 3501.0 4 1301.5 5 516.5 6 1249.0 7 3159.0 8 5370.0 9 5649.0 10 3320.5 11 1041.0 12 467.0 13 411.5 14 418.0 15 397.5 16 379.5 17 419.0 18 458.0 19 458.5 20 437.0 21 460.0 22 516.0 23 554.0 24 587.5 25 638.0 26 735.5 27 886.0 28 1025.5 29 1117.5 30 1226.5 31 1361.5 32 1470.5 33 1526.0 34 1603.5 35 1645.5 36 1718.0 37 1824.0 38 1893.5 39 1953.5 40 1959.0 41 1915.5 42 1911.0 43 2057.0 44 2205.5 45 2249.5 46 2325.0 47 2531.0 48 2775.0 49 2789.5 50 2710.0 51 2534.5 52 2287.5 53 2335.0 54 2320.0 55 2092.0 56 1925.5 57 1724.5 58 1584.0 59 1639.0 60 1615.0 61 1484.0 62 1285.5 63 875.0 64 509.5 65 377.0 66 296.5 67 237.0 68 184.5 69 114.0 70 97.0 71 93.5 72 89.0 73 131.0 74 107.0 75 48.5 76 35.0 77 37.0 78 35.0 79 19.0 80 5.5 81 1.0 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024013595389389686 2 0.001847199645337668 3 0.0 4 0.0895891827988769 5 0.0 6 0.02770799468006502 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.61799911334417E-4 26-27 0.0 28-29 9.23599822668834E-4 30-31 0.006465198758681838 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.00461799911334417 46-47 0.003232599379340919 48-49 0.0 50-51 0.002770799468006502 52-53 0.0 54-55 0.001385399734003251 56-57 0.0 58-59 0.002770799468006502 60-61 4.61799911334417E-4 62-63 0.004156199202009754 64-65 0.003232599379340919 66-67 0.0 68-69 0.001847199645337668 70-71 0.002770799468006502 72-73 0.004156199202009754 74-75 0.0 76-77 0.002770799468006502 78-79 0.001847199645337668 80-81 0.0 82-83 0.002308999556672085 84-85 0.0 86-87 0.002770799468006502 88-89 0.005079799024678587 90-91 0.003694399290675336 92-93 0.005079799024678587 94-95 0.003232599379340919 96-97 0.002308999556672085 98-99 0.005079799024678587 100-101 0.004156199202009754 102-103 0.001385399734003251 104-105 0.001385399734003251 106-107 0.0 108-109 0.0060033988473474215 110-111 0.001847199645337668 112-113 0.004156199202009754 114-115 0.0 116-117 0.0 118-119 4.61799911334417E-4 120-121 9.23599822668834E-4 122-123 0.001385399734003251 124-125 0.0434091916654352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 108272.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.21457071080243 #Duplication Level Percentage of deduplicated Percentage of total 1 77.85135360962568 34.42164179104478 2 12.324532085561497 10.898477907492241 3 4.244652406417113 5.630264518989212 4 2.0366811497326203 3.602039308408453 5 1.0256517379679144 2.2674375646519875 6 0.6412934491978609 1.7012708733559923 7 0.43866978609625673 1.3576917393231862 8 0.27991310160427807 0.9900990099009901 9 0.20262366310160426 0.8063026451898923 >10 0.8501838235294117 7.030441850155164 >50 0.0501336898395722 1.547953302792966 >100 0.03342245989304812 2.449386729717748 >500 0.004177807486631015 1.058445396778484 >1k 0.01253342245989305 14.610425594798285 >5k 0.004177807486631015 11.62812176740062 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6997 6.462427959213832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5593 5.165693808186789 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4924 4.547805526821339 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4113 3.798766070636914 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2127 1.96449682281661 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1978 1.8268804492389539 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1574 1.453746120880745 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1103 1.0187306044037239 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 594 0.5486182946652874 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 552 0.5098271021131964 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.24752475247524752 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 260 0.24013595389389686 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 0.2382887542485592 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 203 0.1874907640017733 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 190 0.17548396630707846 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 166 0.15331757056302647 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 162 0.1496231712723511 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 161 0.1486995714496823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 156 0.14408157233633812 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.12838037535096794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 135 0.12468597606029261 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 125 0.11544997783360425 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 112 0.10344318013890941 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 112 0.10344318013890941 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.235998226688341E-4 0.0 18-19 0.0 0.0 0.0 9.235998226688341E-4 0.0 20-21 0.0 0.0 0.0 0.002770799468006502 0.0 22-23 0.0 0.0 0.0 0.01062139796069159 0.0 24-25 0.0 0.0 0.0 0.018010196542042264 0.0 26-27 0.0 0.0 0.0 0.022166395744052016 0.0 28-29 0.0 0.0 0.0 0.028169794591399438 0.0 30-31 0.0 0.0 0.0 0.05403058962612679 0.0 32-33 0.0 0.0 0.0 0.09189818235554899 0.0 34-35 0.0 0.0 0.0 0.119606177035614 0.0 36-37 0.0 0.0 0.0 0.14777597162701345 0.0 38-39 0.0 0.0 0.0 0.20550096054381556 0.0 40-41 0.0 0.0 0.0 0.2895485444066795 0.0 42-43 0.0 0.0 0.0 0.36482192995418944 0.0 44-45 0.0 0.0 0.0 0.44425151470370916 0.0 46-47 0.0 0.0 0.0 0.5444620954632777 0.0 48-49 0.0 0.0 0.0 0.6437490764001773 0.0 50-51 0.0 0.0 0.0 0.7578136544997783 0.0 52-53 0.0 0.0 0.0 0.864951233929363 0.0 54-55 0.0 0.0 0.0 0.9776304122949608 0.0 56-57 0.0 0.0 0.0 1.1263299837446432 0.0 58-59 0.0 0.0 0.0 1.269949756169647 0.0 60-61 0.0 0.0 0.0 1.4532843209694102 0.0 62-63 0.0 0.0 0.0 1.6435458844391904 0.0 64-65 0.0 0.0 0.0 1.8241096497709473 0.0 66-67 0.0 0.0 0.0 2.0189892123540716 0.0 68-69 0.0 0.0 0.0 2.24573296881927 0.0 70-71 0.0 0.0 0.0 2.4789419240431507 0.0 72-73 0.0 0.0 0.0 2.744015073149106 0.0 74-75 0.0 0.0 0.0 2.982303827397665 0.0 76-77 0.0 0.0 0.0 3.243682577212945 0.0 78-79 0.0 0.0 0.0 3.518915324368258 0.0 80-81 0.0 0.0 0.0 3.808463868774937 0.0 82-83 0.0 0.0 0.0 4.163588000591103 0.0 84-85 0.0 0.0 0.0 4.510399734003251 0.0 86-87 0.0 0.0 0.0 4.850284468745382 0.0 88-89 0.0 0.0 0.0 5.245123392936309 0.0 90-91 0.0 0.0 0.0 5.667208511895966 0.0 92-93 0.0 0.0 0.0 6.102224028372987 0.0 94-95 0.0 0.0 0.0 6.568641938820748 0.0 96-97 0.0 0.0 0.0 7.003657455297768 0.0 98-99 0.0 0.0 0.0 7.511637357765627 0.0 100-101 0.0 0.0 0.0 8.072724250036945 0.0 102-103 0.0 0.0 0.0 8.672140534949017 0.0 104-105 0.0 0.0 0.0 9.254470223141716 0.0 106-107 0.0 0.0 0.0 9.830334712575734 0.0 108-109 0.0 0.0 0.0 10.430674597310478 0.0 110-111 0.0 0.0 0.0 11.102131668390719 0.0 112-113 0.0 0.0 0.0 11.762043741687602 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1220 0.0 79.17633 1 ATGTTAG 25 0.0020345617 71.55194 1 TATTAAG 30 0.0041907546 59.626617 2 GTATCAA 2480 0.0 59.386192 1 GTGGTAT 390 0.0 51.98218 1 TAGGGTA 35 0.0077121705 51.10853 4 TGGTATC 410 0.0 47.99216 2 ATCACCG 25 5.327852E-4 47.59113 62-63 GGGATAA 50 5.3159747E-4 47.59113 7 TATCAAC 3405 0.0 43.253376 2 ATCAACG 3465 0.0 42.848568 3 TCAACGC 3495 0.0 42.31016 4 CAACGCA 3540 0.0 41.714386 5 AACGCAG 3635 0.0 40.460377 6 GGAACCT 60 0.0013044658 39.659275 8 ACGCAGA 4120 0.0 35.664474 7 CGCAGAG 4130 0.0 35.578114 8 GCATGCA 85 1.6791477E-4 35.074482 2 ATGCACT 85 1.6791477E-4 35.074482 4 GCAGAGT 4335 0.0 34.03287 9 >>END_MODULE