FastQCFastQC Report
Fri 27 May 2016
SRR940144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640055
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.063.987751
GTATCAA35400.055.992431
ATCAACG43300.044.951923
TCAACGC44000.044.2367744
CAACGCA45250.043.013455
AACGCAG46350.041.981116
TATCAAC48800.040.3735162
GTACATG104450.039.2644161
TACATGG105200.038.248932
ACATGGG109650.035.719523
CATGGGA65450.033.0130274
ACGCAGA58500.032.6476677
CGCAGAG57900.032.5769278
CATGGGG48400.028.6549034
GCAGAGT65950.026.9773359
ATGGGAG29500.026.6334255
ATGGGAT30000.024.9991025
ATGGGGA26400.024.1243135
GAGTACA77150.024.0717141
AGTACAT75200.022.9545362
Produced by FastQC (version 0.11.5)