FastQCFastQC Report
Fri 27 May 2016
SRR940144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640055
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48120.2934047943514089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37500.22865086841599827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21190.12920298404626673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32000.076.5128251
GTATCAA63300.062.3012431
ATCAACG82200.047.6647873
TATCAAC83600.046.8694342
TCAACGC84100.046.5865144
CAACGCA85000.046.128115
AACGCAG87900.044.6062476
ACGCAGA103550.037.745187
GTACATG101450.037.6986161
CGCAGAG104800.037.294978
TACATGG103750.036.3316962
ACATGGG105000.034.4225463
GCAGAGT117250.033.0811779
CCGTATG902.3957272E-433.050179
CATGGGA63450.032.5632824
TGGTATC13750.032.480942
GAGTACT62750.030.47989512-13
GTGGTAT16650.028.980991
GTACTTT66950.028.03464114-15
CAGAGTA113850.027.14555510-11
Produced by FastQC (version 0.11.5)