FastQCFastQC Report
Fri 27 May 2016
SRR940143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494607
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20560.1375612451968979No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19580.13100433759510025No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA18340.1227078422622134No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17530.11728835740766635No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16880.11293938807994343No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC15350.10270258335468789No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16900.062.6894571
GTATCAA31000.057.791771
TCAACGC38050.045.2068674
ATCAACG39000.044.4109043
TATCAAC40600.043.3937232
CAACGCA40100.043.041345
AACGCAG41450.041.2004856
GTACATG83850.040.1767271
TACATGG88200.036.913052
ACATGGG89850.035.175293
ACGCAGA49650.034.0307357
CGCAGAG49500.034.019368
CATGGGG43400.032.2284474
ATGGGAG23450.029.9482385
CATGGGA51200.029.4111354
GCAGAGT56500.028.6460939
ATGGGGA25250.026.6347855
TAAGGTG8350.024.9467355
GTGGTAT9200.024.5842511
GAGTACA64800.024.1569421
Produced by FastQC (version 0.11.5)