FastQCFastQC Report
Fri 27 May 2016
SRR940143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494607
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49360.3302540400252374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38110.25498341704541727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21660.14492103944381365No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19640.13140578091765928No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG18400.12310928558477245No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA17700.11842578015491696No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16910.11314010974122295No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15830.10591412993516021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.078.8281861
GTATCAA57200.061.1568871
ATCAACG76200.045.8954933
TATCAAC76850.045.508832
TCAACGC76750.045.488974
CAACGCA78850.044.373585
AACGCAG80450.043.4156536
GTGGTAT11500.039.9021381
TGGTATC11150.039.007622
ACGCAGA94150.036.9630787
GTACATG81350.036.7747271
CGCAGAG94200.036.6908538
TACATGG85800.034.234272
ACATGGG84650.034.205523
GCAGAGT104400.032.5932859
CATGGGG43000.031.313164
GAGTACT60050.028.97646712-13
AGTACTT62750.027.16085812-13
CATGGGA48550.026.9973014
CAGAGTA101600.026.99229210-11
Produced by FastQC (version 0.11.5)