FastQCFastQC Report
Fri 27 May 2016
SRR940142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414902
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40290.9710726870441694No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16720.4029867293963394No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15490.37334117454242205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.20703684243508105No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7350.17715026680999368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.1371408187957638No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4690.11303874167875787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7550.074.899791
GTATCAA15900.065.515621
GTGGTAT2700.050.7070121
GAACAAA8850.049.7728351
ATCAACG21500.046.507443
TCAACGC21650.046.185214
CAACGCA22150.045.142665
TATCAAC23000.045.0269972
AACGCAG23100.043.2809266
GTACATG50500.040.4302671
TACATGG50700.039.913752
TGGTATC3500.037.4115942
ACATGGG53750.035.65573
CGCAGAG28600.034.949248
ACGCAGA28750.034.76697
CATGGGA40750.034.6156354
GTCGGGA2450.034.010542
ATGGGAA12750.032.6767925
TGGGATG5900.032.27736
GCAGAGT30700.031.5895759
Produced by FastQC (version 0.11.5)