##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940142_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 414902 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.302266559332082 33.0 31.0 34.0 25.0 34.0 2 30.298834423550623 33.0 31.0 34.0 25.0 34.0 3 30.446023398296465 34.0 31.0 34.0 25.0 34.0 4 33.25306457910543 37.0 35.0 37.0 23.0 37.0 5 33.68390125861047 37.0 35.0 37.0 28.0 37.0 6 33.36863644908918 37.0 35.0 37.0 22.0 37.0 7 33.34794963629965 37.0 35.0 37.0 25.0 37.0 8 33.14068382413196 37.0 35.0 37.0 19.0 37.0 9 34.41319395905539 39.0 37.0 39.0 17.0 39.0 10-11 34.60922458797499 39.0 37.0 39.0 19.5 39.0 12-13 34.5474075805853 39.0 37.0 39.0 18.0 39.0 14-15 35.86204332589382 41.0 37.0 41.0 17.5 41.0 16-17 36.04414536444751 40.5 37.0 41.0 23.5 41.0 18-19 36.13003793666938 41.0 37.0 41.0 25.0 41.0 20-21 36.21634385951381 41.0 37.0 41.0 25.5 41.0 22-23 36.30566133689402 40.0 37.0 41.0 27.0 41.0 24-25 36.412917990272405 40.0 37.0 41.0 27.0 41.0 26-27 36.43664407498638 40.0 37.0 41.0 28.0 41.0 28-29 36.379513716492085 40.0 37.0 41.0 28.0 41.0 30-31 36.28023485063943 40.0 36.0 41.0 28.0 41.0 32-33 36.18746595581607 40.0 36.0 41.0 28.0 41.0 34-35 36.088173833821 40.0 36.0 41.0 27.5 41.0 36-37 35.920688499934926 40.0 35.0 41.0 27.0 41.0 38-39 35.76962993670794 40.0 35.0 41.0 26.5 41.0 40-41 35.63242645251168 40.0 35.0 41.0 25.5 41.0 42-43 35.562237588635384 40.0 35.0 41.0 25.5 41.0 44-45 35.61303030595177 40.0 35.0 41.0 25.0 41.0 46-47 35.49969390362062 40.0 35.0 41.0 25.0 41.0 48-49 35.361893893015704 40.0 35.0 41.0 25.0 41.0 50-51 35.228702199555556 40.0 35.0 41.0 25.0 41.0 52-53 35.0430619760811 40.0 35.0 41.0 25.0 41.0 54-55 34.83855223643174 39.0 34.5 41.0 25.0 41.0 56-57 34.63226376349114 39.0 34.0 41.0 25.0 41.0 58-59 34.37230599033025 39.0 34.0 41.0 25.0 41.0 60-61 34.09905351143161 38.0 34.0 41.0 23.5 41.0 62-63 33.7983572024237 37.5 33.0 40.5 22.5 41.0 64-65 33.488709381974544 37.0 33.0 40.0 21.5 41.0 66-67 33.16222385045143 36.5 33.0 40.0 20.0 41.0 68-69 32.84106487797118 36.0 33.0 39.0 18.5 41.0 70-71 32.73250430222076 35.5 33.0 39.0 18.0 41.0 72-73 32.502909120708026 35.0 33.0 39.0 17.0 41.0 74-75 32.18197791285653 35.0 33.0 37.5 11.5 40.5 76-77 31.85022246217179 35.0 33.0 37.0 7.0 39.0 78-79 31.54340061026459 35.0 33.0 36.5 2.0 39.0 80-81 31.237169259246762 35.0 33.0 36.0 2.0 39.0 82-83 30.964097545926506 35.0 32.0 36.0 2.0 37.0 84-85 30.704888142260103 35.0 32.0 35.5 2.0 37.0 86-87 30.490520653069883 35.0 32.0 35.0 2.0 36.5 88-89 30.30457433321604 35.0 32.0 35.0 2.0 36.0 90-91 30.149267778897183 35.0 32.0 35.0 2.0 36.0 92-93 29.994225142322765 35.0 32.0 35.0 2.0 36.0 94-95 29.870986401608093 35.0 31.5 35.0 2.0 35.5 96-97 29.737130695923376 35.0 31.0 35.0 2.0 35.0 98-99 29.642305893921936 35.0 31.0 35.0 2.0 35.0 100-101 29.55548539173106 35.0 31.0 35.0 2.0 35.0 102-103 28.824301883336304 34.0 29.5 35.0 2.0 35.0 104-105 29.331326433712057 35.0 30.5 35.0 2.0 35.0 106-107 29.362804951530723 35.0 31.0 35.0 2.0 35.0 108-109 29.299686431976706 35.0 31.0 35.0 2.0 35.0 110-111 29.196534362331345 35.0 30.0 35.0 2.0 35.0 112-113 29.078728229798845 35.0 30.0 35.0 2.0 35.0 114-115 28.926140389778794 35.0 30.0 35.0 2.0 35.0 116-117 28.822981089510293 35.0 30.0 35.0 2.0 35.0 118-119 28.675097974943483 35.0 30.0 35.0 2.0 35.0 120-121 28.486428120375415 35.0 29.0 35.0 2.0 35.0 122-123 28.24748856356441 35.0 29.0 35.0 2.0 35.0 124-125 26.334131915488477 33.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07884573477048917 1101 2 0.09779213699653866 1101 3 0.24976192948722442 1101 4 0.12881716482654326 1101 5 -0.17499265821187038 1101 6 -0.058300721590462956 1101 7 0.2791713721104756 1101 8 0.14557678969165977 1101 9 -0.0958033614875049 1101 10-11 -0.11754328873512776 1101 12-13 -0.049463951379806304 1101 14-15 0.006439222186749305 1101 16-17 -0.03755373050463362 1101 18-19 -0.15756589794949605 1101 20-21 -0.09162778330133392 1101 22-23 -0.0520913575718609 1101 24-25 0.09539503473232003 1101 26-27 0.22455925345755645 1101 28-29 0.2523782128846719 1101 30-31 0.1324955703396924 1101 32-33 0.1083061467484967 1101 34-35 0.12211502292095844 1101 36-37 0.22038986508864866 1101 38-39 0.14998894697535547 1101 40-41 0.08286617004284835 1101 42-43 0.26889090242119806 1101 44-45 0.3424483044098068 1101 46-47 0.22194752083780145 1101 48-49 0.2925971580854636 1101 50-51 0.13264188147558542 1101 52-53 0.24356555854573259 1101 54-55 0.09477986807497985 1101 56-57 0.2787665156382104 1101 58-59 0.23780110088948447 1101 60-61 0.17448752398735934 1101 62-63 0.21802247102530004 1101 64-65 0.21585105458571974 1101 66-67 0.3001096705780739 1101 68-69 0.13105763907267942 1101 70-71 0.14307304700028567 1101 72-73 0.3322185021186641 1101 74-75 0.32333343626523003 1101 76-77 0.22922779443536712 1101 78-79 0.15270300494819367 1101 80-81 0.1429273633856205 1101 82-83 -0.03476886418498282 1101 84-85 0.044920779970013314 1101 86-87 0.11208824764166891 1101 88-89 0.11595785825097948 1101 90-91 0.05347750297961085 1101 92-93 -0.0055406185763651195 1101 94-95 -0.05355640626503799 1101 96-97 -0.052169318275137044 1101 98-99 -0.10204285545670899 1101 100-101 -0.07892043884073985 1101 102-103 0.03695006481978069 1101 104-105 0.02222603561938641 1101 106-107 -0.13245659013113809 1101 108-109 -0.12503394097418408 1101 110-111 -0.10604831895485134 1101 112-113 0.047983724770695346 1101 114-115 -0.036827864951739286 1101 116-117 -0.04739868389867041 1101 118-119 -0.3216731547913163 1101 120-121 -0.3644427460352091 1101 122-123 -0.24776034926212276 1101 124-125 -0.6950253765959893 1102 1 0.5104246821389076 1102 2 0.399546522961451 1102 3 0.39186719264511893 1102 4 0.5253083928967186 1102 5 0.4162354119635694 1102 6 0.318892260865681 1102 7 0.3318029510578455 1102 8 0.31224345635832407 1102 9 0.46033698938968826 1102 10-11 0.6298251323174995 1102 12-13 0.42948341704124715 1102 14-15 0.6502988713095519 1102 16-17 0.5589374975655375 1102 18-19 0.4231358564364669 1102 20-21 0.45661783073375517 1102 22-23 0.5672068880421719 1102 24-25 0.7445178417498681 1102 26-27 0.5956118850364973 1102 28-29 0.8234308444636227 1102 30-31 0.7228464475326675 1102 32-33 0.6688324625379636 1102 34-35 0.7300097597630568 1102 36-37 0.8238986370184804 1102 38-39 0.5920942101332543 1102 40-41 0.7153223103937307 1102 42-43 0.7618733585615516 1102 44-45 0.45823777809400923 1102 46-47 0.5044036611886753 1102 48-49 0.4250532984363318 1102 50-51 0.5545717060369881 1102 52-53 0.661986611177305 1102 54-55 0.7789903943907603 1102 56-57 0.5875384454627692 1102 58-59 0.47113443422281875 1102 60-61 0.7323822608294606 1102 62-63 0.8188996640077519 1102 64-65 0.7763773703751937 1102 66-67 0.7571272144377232 1102 68-69 0.6573734285463644 1102 70-71 0.6913186610353819 1102 72-73 0.7269553442239243 1102 74-75 0.9417544888968123 1102 76-77 0.9739646365406358 1102 78-79 0.934281952316617 1102 80-81 0.9850326265435143 1102 82-83 0.8941785042360735 1102 84-85 0.8957979729524688 1102 86-87 1.0761233353609683 1102 88-89 1.1238525950930836 1102 90-91 1.0201441696462794 1102 92-93 0.8725295568622293 1102 94-95 1.0288997340858401 1102 96-97 1.1346727869880233 1102 98-99 1.148834337525745 1102 100-101 0.9886234208083806 1102 102-103 0.8001079595566232 1102 104-105 1.0204716496544748 1102 106-107 1.037718848465353 1102 108-109 1.0425099186749378 1102 110-111 0.9562323827995343 1102 112-113 1.1751767072268393 1102 114-115 1.1315931876798366 1102 116-117 0.9069872810136097 1102 118-119 1.1432391259104335 1102 120-121 1.2223993592279498 1102 122-123 1.2478536858255964 1102 124-125 1.0514658514741875 1103 1 -0.25123032951610114 1103 2 -0.17108898188458 1103 3 0.0882357363820816 1103 4 0.04710326469159298 1103 5 -0.14180041186172332 1103 6 0.005231360362678572 1103 7 -0.13224440509477375 1103 8 -0.24890241531386437 1103 9 0.28922301564414 1103 10-11 0.1611771136694884 1103 12-13 0.030823743381574786 1103 14-15 0.08194222795291495 1103 16-17 -0.01936271099782516 1103 18-19 -0.06046124821326515 1103 20-21 -0.17861097554768435 1103 22-23 -0.3160927070995285 1103 24-25 -0.09162802992231889 1103 26-27 -0.19994387499293964 1103 28-29 0.03776957590761043 1103 30-31 0.175652851654867 1103 32-33 0.17108372005237982 1103 34-35 0.2122634709641389 1103 36-37 0.06776748746626993 1103 38-39 -0.10759907903371868 1103 40-41 0.19219631358352274 1103 42-43 -0.11957983736532896 1103 44-45 -0.12419075828189108 1103 46-47 -0.11015896916342172 1103 48-49 -0.0043418678009388145 1103 50-51 -0.044388544239048144 1103 52-53 0.04931945377330749 1103 54-55 -0.08952267149072668 1103 56-57 -0.06278973512856822 1103 58-59 -0.1499181973561292 1103 60-61 -0.3947923189767053 1103 62-63 -0.297834479042173 1103 64-65 -0.2517848183133182 1103 66-67 -0.4097020089704486 1103 68-69 -0.5857593159021732 1103 70-71 -0.3696953249786077 1103 72-73 -0.5886132976605047 1103 74-75 -0.30790199497020154 1103 76-77 -0.5272468712498224 1103 78-79 -0.40220130127190146 1103 80-81 -0.5140319054157558 1103 82-83 -0.3675961246438213 1103 84-85 -0.24002891945337268 1103 86-87 -0.2947544710431522 1103 88-89 -0.22811955556327845 1103 90-91 -0.12465708173391832 1103 92-93 0.002332648507426427 1103 94-95 0.007359432282378009 1103 96-97 -0.27134794666428874 1103 98-99 -0.008667809450084718 1103 100-101 0.008584161821758585 1103 102-103 -0.05787572342705971 1103 104-105 0.09785785993542007 1103 106-107 0.022260622480811065 1103 108-109 0.16451335383626997 1103 110-111 0.18987856118255309 1103 112-113 0.1942694565827452 1103 114-115 0.18460202095182865 1103 116-117 0.09051690928008327 1103 118-119 0.3202450147584308 1103 120-121 0.05888874703312297 1103 122-123 -0.1308483139290324 1103 124-125 -0.1430900312395913 1104 1 -0.13497761671166586 1104 2 -0.038414759555184474 1104 3 -0.17603964341053668 1104 4 -0.2913582737699443 1104 5 -0.3285619262094457 1104 6 -0.4898494209250046 1104 7 -0.23746504652231692 1104 8 -0.31685515223211524 1104 9 -0.36619053632114174 1104 10-11 -0.43368061480893516 1104 12-13 -0.3958039392805901 1104 14-15 -0.3979769907594104 1104 16-17 -0.4969911170745007 1104 18-19 -0.5114981423536094 1104 20-21 -0.5222690416195377 1104 22-23 -0.5487187017944848 1104 24-25 -0.5026842151164317 1104 26-27 -0.6109216902811028 1104 28-29 -0.553278169450472 1104 30-31 -0.5098668011061633 1104 32-33 -0.4390320262702616 1104 34-35 -0.45318745741781896 1104 36-37 -0.4571963418079008 1104 38-39 -0.5227726985176844 1104 40-41 -0.39440243249798357 1104 42-43 -0.42684412480203093 1104 44-45 -0.4309787978285513 1104 46-47 -0.3561982746854895 1104 48-49 -0.3526539005957261 1104 50-51 -0.16874952808279176 1104 52-53 -0.4030617856769041 1104 54-55 -0.4347119649558806 1104 56-57 -0.30373499676348814 1104 58-59 -0.2835388870113036 1104 60-61 -0.4343751499388375 1104 62-63 -0.415341110341906 1104 64-65 -0.3969741117784693 1104 66-67 -0.4680996458163591 1104 68-69 -0.42341906994123235 1104 70-71 -0.45437099413703663 1104 72-73 -0.4541245105855083 1104 74-75 -0.3763792074129313 1104 76-77 -0.5828714191157474 1104 78-79 -0.3430320222750396 1104 80-81 -0.627595927600467 1104 82-83 -0.4612963898958071 1104 84-85 -0.49552084132460195 1104 86-87 -0.37165343414901386 1104 88-89 -0.37897559607448983 1104 90-91 -0.3947378630215965 1104 92-93 -0.3037862326114542 1104 94-95 -0.33248865066915556 1104 96-97 -0.26570531343545056 1104 98-99 -0.27781418032059335 1104 100-101 -0.2663977528153296 1104 102-103 -0.210811580673262 1104 104-105 -0.4151756582705133 1104 106-107 -0.3143682658722149 1104 108-109 -0.242955901046777 1104 110-111 -0.1097537031048823 1104 112-113 -0.09533145974230806 1104 114-115 -0.011328772392754871 1104 116-117 -0.12078041408620876 1104 118-119 0.030716439885026148 1104 120-121 -0.0804288319396278 1104 122-123 0.07787485944931305 1104 124-125 0.011069602230389108 1105 1 0.2463734857003992 1105 2 0.31548829726365213 1105 3 0.16595851443875276 1105 4 0.15263170953556227 1105 5 0.49829029876320874 1105 6 0.4683020959709765 1105 7 0.22026910810321 1105 8 0.3821201514349113 1105 9 0.2880176267091983 1105 10-11 0.23129981233976338 1105 12-13 0.2884427937802556 1105 14-15 0.1670768345816782 1105 16-17 0.24646349118063426 1105 18-19 0.2024273656770177 1105 20-21 0.3780072809871058 1105 22-23 0.40387326182303696 1105 24-25 0.35721590577470863 1105 26-27 0.41390992563047035 1105 28-29 0.2753931793492512 1105 30-31 0.39826600644665433 1105 32-33 0.5121912769362282 1105 34-35 0.32443760734528837 1105 36-37 0.36588235256778745 1105 38-39 0.41261818283629736 1105 40-41 0.4088832159966316 1105 42-43 0.5364596573636433 1105 44-45 0.5222406483748969 1105 46-47 0.41269672272398594 1105 48-49 0.35259335057001095 1105 50-51 0.44142259787425786 1105 52-53 0.40412116291557965 1105 54-55 0.42640714160102533 1105 56-57 0.4555318848207648 1105 58-59 0.4498313852815983 1105 60-61 0.48399166832147245 1105 62-63 0.42417014404758646 1105 64-65 0.37599808325923334 1105 66-67 0.2989776669462927 1105 68-69 0.31422988521390494 1105 70-71 0.3080966243074954 1105 72-73 0.335759491486904 1105 74-75 0.39767956870819177 1105 76-77 0.4196269977859828 1105 78-79 0.5034937966185211 1105 80-81 0.2959821623195147 1105 82-83 0.38306202355189 1105 84-85 0.3786055762679368 1105 86-87 0.34981193917434084 1105 88-89 0.1547256876742864 1105 90-91 0.3811932865955825 1105 92-93 0.34679807456717526 1105 94-95 0.2844836019357615 1105 96-97 0.413828104329788 1105 98-99 0.46590811545972954 1105 100-101 0.432370308896715 1105 102-103 0.4155225394119384 1105 104-105 0.5702900604561023 1105 106-107 0.2944625440984261 1105 108-109 0.2339063981875782 1105 110-111 0.6120944921630915 1105 112-113 0.6102439245188158 1105 114-115 0.5446236770920159 1105 116-117 0.39687832749458707 1105 118-119 0.3253540542998543 1105 120-121 0.3640403983867806 1105 122-123 0.3117862635135573 1105 124-125 0.3588172851501952 1106 1 -0.14714073310568665 1106 2 0.0015464032081453638 1106 3 0.041325308925582505 1106 4 0.14772022452356737 1106 5 0.2872730743789944 1106 6 -0.07754507821581313 1106 7 -0.1570719666117597 1106 8 -0.01677277021493495 1106 9 -0.13696257351073626 1106 10-11 -0.07500990750047265 1106 12-13 0.17646862750768122 1106 14-15 0.1088312945177492 1106 16-17 0.23419047642908453 1106 18-19 0.11692365357436785 1106 20-21 -0.26660233572334135 1106 22-23 0.09539981062165737 1106 24-25 0.07864154691722547 1106 26-27 0.2782686121785929 1106 28-29 0.09700428317986365 1106 30-31 0.20670669542202091 1106 32-33 0.13692419357203534 1106 34-35 0.12982713596807827 1106 36-37 0.11390522345042342 1106 38-39 0.11462579506829229 1106 40-41 0.09446128410787935 1106 42-43 0.1790579582861156 1106 44-45 0.05517808106988298 1106 46-47 0.1379555443094489 1106 48-49 0.2784453108906817 1106 50-51 0.26388551958108764 1106 52-53 0.12859938885529232 1106 54-55 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-0.804650178648167 2216 32-33 -1.045641221672561 2216 34-35 -0.987736529035864 2216 36-37 -0.9289691092703194 2216 38-39 -0.9522377736724081 2216 40-41 -0.927558931171724 2216 42-43 -0.7728432406287382 2216 44-45 -0.9381184972096293 2216 46-47 -0.9624316964361554 2216 48-49 -0.7005539890130521 2216 50-51 -0.7858803857308914 2216 52-53 -0.8127434832896299 2216 54-55 -0.7394643964324459 2216 56-57 -0.6499109472497793 2216 58-59 -0.5967130691510008 2216 60-61 -0.5978808970652807 2216 62-63 -0.49270627391397426 2216 64-65 -0.7030085945370885 2216 66-67 -0.6669281161965515 2216 68-69 -0.5157709709138274 2216 70-71 -0.5830403133235933 2216 72-73 -0.5590095680567728 2216 74-75 -0.6311537432219474 2216 76-77 -0.6148342838372329 2216 78-79 -0.6018516509222493 2216 80-81 -0.6788337702176186 2216 82-83 -0.6682371502848454 2216 84-85 -0.7192829987074489 2216 86-87 -0.7705703218590045 2216 88-89 -0.6671055695492925 2216 90-91 -0.7263470584238938 2216 92-93 -0.8730170018422214 2216 94-95 -0.773293248370301 2216 96-97 -0.6276416529579976 2216 98-99 -0.8096002103824844 2216 100-101 -0.9782456750080826 2216 102-103 -0.8986099891613257 2216 104-105 -0.9400209279433689 2216 106-107 -0.9570517318315446 2216 108-109 -0.9427127533898378 2216 110-111 -0.8344221381181427 2216 112-113 -1.0598097974965057 2216 114-115 -1.0341625478127376 2216 116-117 -1.3522569834938096 2216 118-119 -1.3170510225376084 2216 120-121 -1.247404959260578 2216 122-123 -1.48655926963999 2216 124-125 -1.612029425178985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12342.0 3 2616.0 4 2392.0 5 1940.0 6 2209.0 7 1718.0 8 1446.0 9 1417.0 10 1785.0 11 2192.0 12 2184.0 13 2027.0 14 1923.0 15 1854.0 16 1801.0 17 1765.0 18 1545.0 19 1444.0 20 1321.0 21 1350.0 22 1488.0 23 2701.0 24 4798.0 25 4265.0 26 7028.0 27 5132.0 28 4571.0 29 5866.0 30 6131.0 31 6289.0 32 8408.0 33 11313.0 34 16991.0 35 29210.0 36 61090.0 37 126223.0 38 65856.0 39 271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.66002786656831 19.307240968676627 12.292145773972239 20.74058539078282 2 14.870692252623154 24.12917035262243 38.67951588837761 22.320621506376803 3 18.109133507525286 32.88901530125244 27.075599949863573 21.926251241358695 4 11.441768965949526 19.708965370929324 35.409695368325636 33.43957029479551 5 12.026132761176939 44.97872494304553 29.59872711273972 13.396415183037814 6 28.014782814134797 38.99027497191458 18.468151376788185 14.526790837162432 7 27.56335780709916 38.67248701990522 18.48355854661505 15.280596626380566 8 27.05712219812003 37.40684502289709 18.47240298867197 17.06362979031092 9 29.0452590778487 20.039001484852577 17.186204369709007 33.729535067589715 10-11 23.942848948121295 32.43078762257457 24.125061309880323 19.501302119423812 12-13 25.136693023082053 30.117537320178023 24.883372560645668 19.862397096094263 14-15 22.658883736963332 29.975448336043215 23.664573889419884 23.70109403757357 16-17 21.213888714816655 34.02812257470511 23.64026821049993 21.117720499978308 18-19 21.638937263858175 32.09058204629133 26.08500201869249 20.185478671158005 20-21 24.092119692934357 30.58424420636547 25.263861940973015 20.05977415972716 22-23 23.162179847939445 31.370051373763108 24.77871200444444 20.689056773853007 24-25 22.16397188557545 31.024450679239095 25.64055283988469 21.171024595300764 26-27 22.0271292980029 31.300523979156523 25.344780213158767 21.327566509681805 28-29 21.793708354272948 31.310958377472232 25.381703538463206 21.513629729791617 30-31 21.97544224565226 30.996422025092823 25.619939286501914 21.408196442753006 32-33 21.375813003966048 31.367582035519703 25.55492755422135 21.701677406292895 34-35 21.870277487882653 31.26420519593831 25.616472363286665 21.249044952892376 36-37 22.04637485537987 30.798785190898574 25.41192634014655 21.74291361357501 38-39 21.72526117770689 30.92158462471032 25.642354353283142 21.710799844299643 40-41 21.95789833989389 30.87238130132842 25.536756393228444 21.632963965549244 42-43 21.701492249510096 30.900724793131456 25.602166425229644 21.7956165321288 44-45 21.486890198710775 30.736841090432744 25.80142902076286 21.974839690093624 46-47 21.800622857721365 30.998259662249133 25.458947794688353 21.74216968534115 48-49 21.482845515032242 30.93121936699113 25.58114591405223 22.0047892039244 50-51 21.6714352172121 30.904787271224066 25.462493160524595 21.96128435103924 52-53 22.070828924502333 30.634486016674416 25.654334794775448 21.6403502640478 54-55 21.41961903430523 30.708640541055164 25.770620376820048 22.10112004781956 56-57 21.5107141996288 31.05129317617567 25.488345746860464 21.94964687733507 58-59 21.751502996350382 30.767070519094204 25.362430635573403 22.118995848982014 60-61 21.453887675059562 30.922800856603306 25.610607033836313 22.01270443450082 62-63 21.50503941505907 30.692887934930503 25.60285576896424 22.199216881046183 64-65 21.311967336392733 30.785425745247004 25.574481315799623 22.328125602560643 66-67 21.209721930985282 31.22353258760608 25.268440118872874 22.29830536253576 68-69 21.354345549485842 30.743079984044407 25.589085602245355 22.3134888642244 70-71 21.03715487424226 30.847222724369406 25.60552924304325 22.510093158345086 72-73 21.42043694813576 30.938622633619385 25.30172896875275 22.339211449492108 74-75 20.924414106281592 30.990240527961166 25.557813241933193 22.527532123824052 76-77 21.177292997468964 30.88694708930939 25.60106062432204 22.3346992888996 78-79 21.188296358248383 30.937474767803536 25.449118033051615 22.425110840896465 80-81 21.13102268071055 31.34915278748584 25.35925184988792 22.16057268191569 82-83 21.210057905868318 31.145403382763213 25.304201640164138 22.340337071204335 84-85 21.28502634357029 30.656395968204542 25.58531894278649 22.47325874543868 86-87 21.13804286129191 30.66825099753794 25.477558861065354 22.7161472801048 88-89 21.24600042180109 30.68169081980055 25.595251724865175 22.47705703353319 90-91 21.260769641451834 30.862547498502586 25.458353770896025 22.41832908914955 92-93 21.188509030426843 30.710923893719983 25.449472396483046 22.65109467937013 94-95 21.0064063320977 30.454782093120787 25.746079797156916 22.792731777624596 96-97 21.05240324127858 30.456521843923085 25.650762356048958 22.84031255874938 98-99 20.87080283350165 30.688153331099826 25.278927337891936 23.162116497506585 100-101 20.619326973598582 30.989486673961565 25.54555051554343 22.845635836896424 102-103 20.774594421319783 30.805059520222894 25.472341594741877 22.948004463715442 104-105 20.494993064167595 31.055897626872703 25.576168214725175 22.872941094234523 106-107 20.425305252806687 31.304500821880833 25.509517910253503 22.760676015058976 108-109 20.426871887819292 31.36318937966074 25.206795821663913 23.00314291085606 110-111 20.05864049496145 32.024989093783816 25.128886168025627 22.78748424322911 112-113 20.20815682625919 32.11402346946523 25.070653045040793 22.60716665923479 114-115 20.048542343795003 32.27101764179173 24.976409722448185 22.704030291965086 116-117 20.01858270145721 32.40813493306853 25.084236759523936 22.48904560595032 118-119 19.976234263635774 32.34261225453923 24.969569734117812 22.711583747707184 120-121 19.715181635879837 32.41450869615518 25.064232964405537 22.806076703559448 122-123 19.925789704556742 32.52081214359053 24.797422041831567 22.755976110021162 124-125 19.869100697430135 32.523061746023515 24.484103103401285 23.12373445314507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3333.0 1 3175.5 2 3768.0 3 3602.5 4 2055.5 5 1169.0 6 809.5 7 737.5 8 926.0 9 1002.0 10 732.5 11 493.5 12 427.5 13 410.0 14 407.5 15 409.5 16 419.5 17 452.5 18 508.5 19 578.5 20 662.0 21 793.0 22 1023.5 23 1241.5 24 1462.5 25 1796.0 26 2306.0 27 2914.0 28 3446.5 29 4041.0 30 4686.5 31 5376.0 32 6272.0 33 7316.0 34 8396.0 35 9092.5 36 9583.0 37 10499.0 38 10982.5 39 10840.0 40 10981.0 41 11365.5 42 11597.5 43 12295.5 44 13028.5 45 13329.0 46 13677.5 47 14003.0 48 14052.5 49 13746.0 50 13429.5 51 13129.5 52 12985.5 53 12926.5 54 12644.0 55 12083.5 56 11336.0 57 10384.0 58 9936.0 59 9617.5 60 8589.5 61 7465.5 62 6511.5 63 5031.0 64 3676.0 65 2780.5 66 1915.5 67 1440.0 68 1077.0 69 766.0 70 552.0 71 440.0 72 391.0 73 355.0 74 275.0 75 191.0 76 138.0 77 89.5 78 65.0 79 34.0 80 16.0 81 10.0 82 9.0 83 7.5 84 6.5 85 5.0 86 5.0 87 4.5 88 2.5 89 0.0 90 1.0 91 1.5 92 1.5 93 2.5 94 1.5 95 1.0 96 1.0 97 1.0 98 2.5 99 3.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016389412439564044 2 0.009399810075632318 3 0.008194706219782022 4 0.025548201744026302 5 0.023379014803495766 6 0.023137994032325707 7 0.00867674776212214 8 4.820415423401189E-4 9 0.011086955473822734 10-11 3.6153115675508917E-4 12-13 0.0030127596396257433 14-15 0.015063798198128715 16-17 9.640830846802378E-4 18-19 0.007110112749516753 20-21 0.001687145398190416 22-23 3.6153115675508917E-4 24-25 0.0062665400504215455 26-27 0.0 28-29 0.0044588842666461 30-31 6.025519279251486E-4 32-33 0.001325614241435327 34-35 2.4102077117005944E-4 36-37 0.005302456965741308 38-39 6.025519279251486E-4 40-41 0.011810017787332913 42-43 0.0060255192792514865 44-45 6.025519279251486E-4 46-47 0.009640830846802378 48-49 0.0025307180972856244 50-51 0.007471643906271842 52-53 0.0036153115675508917 54-55 0.002169186940530535 56-57 0.007712664677441902 58-59 0.015425329354883804 60-61 0.0018076557837754458 62-63 0.005422967351326338 64-65 0.0014461246270203567 66-67 0.0012051038558502972 68-69 1.2051038558502972E-4 70-71 0.004097353109891011 72-73 3.6153115675508917E-4 74-75 0.005543477736911367 76-77 0.012533080100843091 78-79 0.001325614241435327 80-81 0.0028922492540407133 82-83 0.0010845934702652675 84-85 0.0 86-87 0.001325614241435327 88-89 0.003494801181965862 90-91 0.004940925808986219 92-93 0.013135632028768239 94-95 0.0 96-97 9.640830846802378E-4 98-99 0.002169186940530535 100-101 0.0 102-103 2.4102077117005944E-4 104-105 0.0066280712071766345 106-107 0.0 108-109 0.0 110-111 2.4102077117005944E-4 112-113 0.005422967351326338 114-115 0.0015666350126053864 116-117 0.0 118-119 0.004579394652231129 120-121 0.0014461246270203567 122-123 0.0 124-125 0.07435490790596333 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 414902.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.267432788646694 #Duplication Level Percentage of deduplicated Percentage of total 1 70.36853103463179 33.96508342149889 2 13.236293372058352 12.777638014132725 3 5.883598823380474 8.519586322886333 4 3.1412103373588773 6.064726353338873 5 1.9248824791242303 4.64545678435862 6 1.249054025329118 3.617317873017708 7 0.91058264574014 3.07660406562392 8 0.6314885231843977 2.438426387968374 9 0.48148975265691274 2.091624684731069 >10 2.0789181662468934 16.51564903525805 >50 0.06846417616797355 2.208768673572564 >100 0.022488233047841755 1.8085997067192432 >500 0.0014992155365227836 0.5217392858940681 >1k 0.0014992155365227836 1.7487793909995348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4029 0.9710726870441694 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1672 0.4029867293963394 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1549 0.37334117454242205 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 859 0.20703684243508105 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 735 0.17715026680999368 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 569 0.1371408187957638 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 469 0.11303874167875787 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2051038558502972E-4 0.0 14-15 0.0 0.0 0.0 2.4102077117005944E-4 0.0 16-17 0.0 0.0 0.0 7.230623135101783E-4 0.0 18-19 0.0 0.0 0.0 8.43572699095208E-4 0.0 20-21 0.0 0.0 0.0 0.0015666350126053864 0.0 22-23 0.0 0.0 0.0 0.003856332338720951 0.0 24-25 0.0 0.0 0.0 0.008074195834196992 0.0 26-27 0.0 0.0 0.0 0.010604913931482616 0.0 28-29 0.0 0.0 0.0 0.014943287812543685 0.0 30-31 0.0 0.0 0.0 0.028560961383652043 0.0 32-33 0.0 0.0 0.0 0.04856568539076697 0.0 34-35 0.0 0.0 0.0 0.07170367942309269 0.0 36-37 0.0 0.0 0.0 0.10713373278509142 0.0 38-39 0.0 0.0 0.0 0.17980149529286435 0.0 40-41 0.0 0.0 0.0 0.26982275332488154 0.0 42-43 0.0 0.0 0.0 0.3496006285821712 0.0 44-45 0.0 0.0 0.0 0.42636574419983514 0.0 46-47 0.0 0.0 0.0 0.5206048657273283 0.0 48-49 0.0 0.0 0.0 0.6393075955285827 0.0 50-51 0.0 0.0 0.0 0.7821124024468429 0.0 52-53 0.0 0.0 0.0 0.9179276070011713 0.0 54-55 0.0 0.0 0.0 1.042053304153752 0.0 56-57 0.0 0.0 0.0 1.1870272980125427 0.0 58-59 0.0 0.0 0.0 1.3567059209162646 0.0 60-61 0.0 0.0 0.0 1.5672375645333116 0.0 62-63 0.0 0.0 0.0 1.7788538016206235 0.0 64-65 0.0 0.0 0.0 1.958052744985563 0.0 66-67 0.0 0.0 0.0 2.159184578526977 0.0 68-69 0.0 0.0 0.0 2.419848542547397 0.0 70-71 0.0 0.0 0.0 2.71654511185774 0.0 72-73 0.0 0.0 0.0 3.029390072836477 0.0 74-75 0.0 0.0 0.0 3.3117459062622014 0.0 76-77 0.0 0.0 0.0 3.6031400186068034 0.0 78-79 0.0 0.0 0.0 3.9397255255457915 0.0 80-81 0.0 0.0 0.0 4.340181536844845 0.0 82-83 0.0 0.0 0.0 4.782213631170734 0.0 84-85 0.0 0.0 0.0 5.196528336812067 0.0 86-87 0.0 0.0 0.0 5.612771208622759 0.0 88-89 0.0 0.0 0.0 6.068300466134172 0.0 90-91 0.0 0.0 0.0 6.603246067746118 0.0 92-93 0.0 0.0 0.0 7.202785236031641 0.0 94-95 0.0 0.0 0.0 7.775450588331703 0.0 96-97 0.0 0.0 0.0 8.323170290815662 0.0 98-99 0.0 0.0 0.0 8.918371085220123 0.0 100-101 0.0 0.0 0.0 9.599857315703467 0.0 102-103 0.0 0.0 0.0 10.313881350294768 0.0 104-105 0.0 0.0 0.0 11.055020221642701 0.0 106-107 0.0 0.0 0.0 11.76566996543762 0.0 108-109 0.0 0.0 0.0 12.4758376676902 0.0 110-111 0.0 0.0 0.0 13.274942034504534 0.0 112-113 0.0 0.0 0.0 14.144665487271693 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 755 0.0 74.89979 1 GTATCAA 1590 0.0 65.51562 1 GTGGTAT 270 0.0 50.707012 1 GAACAAA 885 0.0 49.772835 1 ATCAACG 2150 0.0 46.50744 3 TCAACGC 2165 0.0 46.18521 4 CAACGCA 2215 0.0 45.14266 5 TATCAAC 2300 0.0 45.026997 2 AACGCAG 2310 0.0 43.280926 6 GTACATG 5050 0.0 40.430267 1 TACATGG 5070 0.0 39.91375 2 TGGTATC 350 0.0 37.411594 2 ACATGGG 5375 0.0 35.6557 3 CGCAGAG 2860 0.0 34.94924 8 ACGCAGA 2875 0.0 34.7669 7 CATGGGA 4075 0.0 34.615635 4 GTCGGGA 245 0.0 34.01054 2 ATGGGAA 1275 0.0 32.676792 5 TGGGATG 590 0.0 32.2773 6 GCAGAGT 3070 0.0 31.589575 9 >>END_MODULE