FastQCFastQC Report
Fri 27 May 2016
SRR940142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414902
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84102.0269846855402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66581.6047162944502555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45121.0874857195193082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.43431942964844716No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17360.41841205875122317No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.4147967471836723No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.3381521419515934No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.3145321063769276No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.15690452203170868No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.14822777426958655No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4600.11086955473822735No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.10918240934003692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.098.655741
GTATCAA45800.068.439421
GTGGTAT4300.058.2058871
TGGTATC4200.056.7334982
GTGTACG453.1445472E-452.970441
TATCAAC61700.050.6877442
ATCAACG61700.050.5911943
TCAACGC62700.049.7783164
ATAAGAC602.175718E-549.6418083
CAACGCA63600.049.0147635
AACGCAG64850.048.161736
ACGCAGA73950.042.1546867
CGCAGAG74200.042.0126578
GCAGAGT79100.039.109269
GTACATG43100.038.160841
TACATGG44150.037.2397882
ACATGGG44000.036.4190373
CATGGGA32700.034.426314
ATGGGAG11950.033.35495
ATATGGG1102.0202911E-532.492823
Produced by FastQC (version 0.11.5)