##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940142_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 414902 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65716964487999 34.0 31.0 34.0 31.0 34.0 2 32.670659577442386 34.0 31.0 34.0 31.0 34.0 3 32.74607015632607 34.0 31.0 34.0 31.0 34.0 4 36.219463873396606 37.0 35.0 37.0 35.0 37.0 5 36.21984709642277 37.0 37.0 37.0 35.0 37.0 6 36.30626750413351 37.0 37.0 37.0 35.0 37.0 7 36.276062781090474 37.0 37.0 37.0 35.0 37.0 8 36.27356580590115 37.0 37.0 37.0 35.0 37.0 9 38.05690982448868 39.0 39.0 39.0 35.0 39.0 10-11 38.03618926879119 39.0 39.0 39.0 35.0 39.0 12-13 38.027334165658395 39.0 39.0 39.0 35.0 39.0 14-15 39.354509016587045 41.0 39.0 41.0 36.0 41.0 16-17 39.36673117989308 41.0 39.0 41.0 36.0 41.0 18-19 39.508430906575526 41.0 39.5 41.0 36.0 41.0 20-21 39.51694014490168 41.0 40.0 41.0 36.0 41.0 22-23 39.44471104019745 41.0 39.0 41.0 36.0 41.0 24-25 39.38746981214841 41.0 39.0 41.0 35.5 41.0 26-27 39.2103773903235 41.0 39.0 41.0 35.0 41.0 28-29 39.053719914582246 41.0 39.0 41.0 35.0 41.0 30-31 38.703280533716395 40.0 38.0 41.0 35.0 41.0 32-33 38.27362244578238 40.0 36.5 41.0 35.0 41.0 34-35 38.032504061199994 40.0 36.0 41.0 34.0 41.0 36-37 37.82445613662985 40.0 35.5 41.0 34.0 41.0 38-39 37.610504408269904 40.0 35.0 41.0 33.0 41.0 40-41 37.40628268844209 40.0 35.0 41.0 33.0 41.0 42-43 37.0897296228989 40.0 35.0 41.0 33.0 41.0 44-45 36.881538290969914 40.0 35.0 41.0 32.5 41.0 46-47 36.262999455293055 40.0 35.0 41.0 29.5 41.0 48-49 35.66043065591393 40.0 35.0 41.0 24.5 41.0 50-51 35.405872953131095 40.0 35.0 41.0 24.0 41.0 52-53 35.2917435924628 40.0 35.0 41.0 24.0 41.0 54-55 35.10000433837388 39.0 35.0 41.0 24.0 41.0 56-57 34.914935334127094 39.0 35.0 41.0 23.5 41.0 58-59 34.685690837836404 39.0 35.0 41.0 23.0 41.0 60-61 34.42227080129766 38.0 35.0 41.0 22.5 41.0 62-63 34.16044993757562 37.5 35.0 41.0 22.0 41.0 64-65 33.859722295867456 37.0 35.0 40.0 20.5 41.0 66-67 33.55056495268762 36.5 34.5 40.0 20.0 41.0 68-69 33.216416165745166 36.0 34.0 39.0 18.5 41.0 70-71 32.95863239994023 35.5 34.0 39.0 18.0 41.0 72-73 32.77517702975642 35.0 34.0 39.0 16.5 41.0 74-75 32.48067977498301 35.0 34.0 37.5 14.0 40.5 76-77 32.17879764378094 35.0 34.0 37.0 10.5 39.0 78-79 31.875418773589907 35.0 34.0 36.5 7.0 39.0 80-81 31.60447165836752 35.0 34.0 36.0 6.0 39.0 82-83 31.35904502750047 35.0 34.0 36.0 2.0 37.0 84-85 31.14139604051077 35.0 34.0 35.5 2.0 37.0 86-87 30.9405305349215 35.0 34.0 35.0 2.0 36.5 88-89 30.77322355640609 35.0 33.0 35.0 2.0 36.0 90-91 30.638720709950782 35.0 33.0 35.0 2.0 36.0 92-93 30.49846469768765 35.0 33.0 35.0 2.0 36.0 94-95 30.378494198630037 35.0 33.0 35.0 2.0 35.5 96-97 30.288747704277156 35.0 33.0 35.0 2.0 35.0 98-99 30.202386346655356 35.0 33.0 35.0 2.0 35.0 100-101 30.152091337231443 35.0 33.0 35.0 2.0 35.0 102-103 29.37186010190358 34.5 31.5 35.0 2.0 35.0 104-105 29.9398508563468 35.0 33.0 35.0 2.0 35.0 106-107 29.967292276248365 35.0 33.0 35.0 2.0 35.0 108-109 29.93806489243243 35.0 33.0 35.0 2.0 35.0 110-111 29.870476642677065 35.0 33.0 35.0 2.0 35.0 112-113 29.777007582513463 35.0 33.0 35.0 2.0 35.0 114-115 29.65824580262327 35.0 33.0 35.0 2.0 35.0 116-117 29.54471778877904 35.0 33.0 35.0 2.0 35.0 118-119 29.42442673209577 35.0 33.0 35.0 2.0 35.0 120-121 29.27639177444312 35.0 33.0 35.0 2.0 35.0 122-123 29.07911868344814 35.0 32.0 35.0 2.0 35.0 124-125 27.468629941528363 33.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.320699164225708 1101 2 0.22094890094660968 1101 3 0.19336025863302098 1101 4 0.1602257950435373 1101 5 0.0426541205796056 1101 6 0.10474397819735515 1101 7 0.1556646242264179 1101 8 0.19281757584970904 1101 9 0.26516400268479146 1101 10-11 0.17221571451045747 1101 12-13 0.11059086928984385 1101 14-15 0.29676490030113456 1101 16-17 0.3878143217807519 1101 18-19 0.30535539913510235 1101 20-21 0.2393195739760472 1101 22-23 0.25513993622960385 1101 24-25 0.24612134496810256 1101 26-27 0.2057958335472918 1101 28-29 0.3020975144051903 1101 30-31 0.34709476955728036 1101 32-33 0.44722435357108736 1101 34-35 0.4510081190017061 1101 36-37 0.4939710487181017 1101 38-39 0.44114690889932007 1101 40-41 0.28589627088815206 1101 42-43 0.42547369172950056 1101 44-45 0.4469065530448688 1101 46-47 0.28913057457365454 1101 48-49 0.35947410592414286 1101 50-51 0.3718832467591824 1101 52-53 0.3272741535107073 1101 54-55 0.2293845591023782 1101 56-57 0.22018498145410348 1101 58-59 0.10490518861892895 1101 60-61 0.06823144582211427 1101 62-63 0.14020853138762845 1101 64-65 0.16080644010707346 1101 66-67 0.15372031990642654 1101 68-69 0.16166387358104117 1101 70-71 0.16384033697123357 1101 72-73 0.26860552558888884 1101 74-75 0.3946647877173888 1101 76-77 0.3863054053074464 1101 78-79 0.2678532243568803 1101 80-81 0.25237250533498923 1101 82-83 0.21565767657464363 1101 84-85 0.2553832392421853 1101 86-87 0.3015451043842212 1101 88-89 0.17175009718251033 1101 90-91 0.257313462303113 1101 92-93 0.25881223518791074 1101 94-95 0.30951318179232956 1101 96-97 0.06702222387988854 1101 98-99 -0.10557487798632437 1101 100-101 -0.07866606281087485 1101 102-103 -0.0022815658370802794 1101 104-105 0.0171947335828726 1101 106-107 -0.04634784305447326 1101 108-109 -0.08166968963450927 1101 110-111 -0.1884824753576595 1101 112-113 0.0184975012561317 1101 114-115 0.11487519657292111 1101 116-117 0.1842627283556233 1101 118-119 0.08102163599021495 1101 120-121 0.028325711037609835 1101 122-123 0.2010847288230515 1101 124-125 0.27556590752148225 1102 1 0.006664076506410765 1102 2 0.03147521673608367 1102 3 0.14072867968565816 1102 4 -0.0906513979389203 1102 5 -0.12050377415723545 1102 6 0.01000713609209214 1102 7 0.09776988738430958 1102 8 0.029659681112867986 1102 9 0.06340961671988055 1102 10-11 0.06870694258063281 1102 12-13 0.015854027184580843 1102 14-15 0.1809754266169179 1102 16-17 0.10711256739478614 1102 18-19 0.08254838159124489 1102 20-21 0.1437055388883266 1102 22-23 0.07706976079100514 1102 24-25 0.19875292391546395 1102 26-27 0.24000635986308083 1102 28-29 0.053851900370105454 1102 30-31 0.09709476955728036 1102 32-33 0.34020680971143236 1102 34-35 0.28346425935257713 1102 36-37 0.3088833294198565 1102 38-39 0.3086907685484377 1102 40-41 0.08940504281797956 1102 42-43 0.28950877944879494 1102 44-45 0.5012925179571468 1102 46-47 0.5198323289596161 1102 48-49 0.4112284918890552 1102 50-51 0.25433938711006476 1102 52-53 0.13604608333528034 1102 54-55 0.005700348576063163 1102 56-57 0.17807971829620328 1102 58-59 0.1610455394961221 1102 60-61 0.20068758617299665 1102 62-63 0.06038396998411599 1102 64-65 0.040631001510583076 1102 66-67 0.07126417955555553 1102 68-69 0.049383171826661965 1102 70-71 0.06296314398877456 1102 72-73 0.223868683483623 1102 74-75 0.24817355964720633 1102 76-77 0.23893698425481347 1102 78-79 0.2441690138305681 1102 80-81 0.08044268077358652 1102 82-83 0.0209208344693792 1102 84-85 0.28871657257551675 1102 86-87 0.17347492894562322 1102 88-89 0.23578518490180755 1102 90-91 0.18889240967153498 1102 92-93 0.22372451588966413 1102 94-95 0.383197392318646 1102 96-97 0.22667134668690636 1102 98-99 0.1882847711364839 1102 100-101 0.17308832315403677 1102 102-103 0.15912194293484916 1102 104-105 0.21807192656532948 1102 106-107 0.02382759554201641 1102 108-109 0.1314882051023325 1102 110-111 0.24397366499322004 1102 112-113 0.35797118546665985 1102 114-115 0.4017173018360758 1102 116-117 0.052683780987202056 1102 118-119 0.2643549693235485 1102 120-121 0.23095728998497833 1102 122-123 0.3019619218055105 1102 124-125 0.2396009952407816 1103 1 -0.027110920426480334 1103 2 -0.047895412634545664 1103 3 -0.21902472480459068 1103 4 -0.24146724875477332 1103 5 -0.15409474459031003 1103 6 -0.010395268520838385 1103 7 -0.05043278713414878 1103 8 -0.04837982324242773 1103 9 0.014023038385936104 1103 10-11 0.011437895837900669 1103 12-13 -0.015850671625379675 1103 14-15 0.05489580448466569 1103 16-17 0.11589676565266416 1103 18-19 0.04896967959148668 1103 20-21 0.10371044626165826 1103 22-23 0.1259042596255 1103 24-25 0.16427862628327716 1103 26-27 0.15094366816881433 1103 28-29 0.2256007655400225 1103 30-31 0.19820506277283556 1103 32-33 0.11070736179092933 1103 34-35 0.11789561746814314 1103 36-37 -0.10080873290378634 1103 38-39 0.14285835534760594 1103 40-41 0.31480683400923937 1103 42-43 0.18137480815692442 1103 44-45 0.15095206893249014 1103 46-47 0.0667314824377101 1103 48-49 0.088255850495365 1103 50-51 -0.09079004486147824 1103 52-53 0.1064024813232507 1103 54-55 -0.05262991764893599 1103 56-57 0.03418939809009913 1103 58-59 -0.05536962428745795 1103 60-61 0.15818359893216183 1103 62-63 -0.21326650235056377 1103 64-65 -0.20476526888568714 1103 66-67 -0.2492455738489383 1103 68-69 -0.07931576081963954 1103 70-71 0.07675286304164786 1103 72-73 -0.1285205939056553 1103 74-75 -0.12253555579874131 1103 76-77 -0.17445707782345465 1103 78-79 -0.3640109640862512 1103 80-81 -0.2840739429535688 1103 82-83 -0.20439201058030676 1103 84-85 -0.3309589273631417 1103 86-87 -0.32187533482569464 1103 88-89 -0.33438411947802393 1103 90-91 -0.2653248642825794 1103 92-93 -0.46172818929988324 1103 94-95 -0.10741725619073961 1103 96-97 9.045696043408213E-4 1103 98-99 0.07138500423671701 1103 100-101 0.1272718589165187 1103 102-103 0.15971082773426204 1103 104-105 -0.14104965360888144 1103 106-107 -0.022614558794874284 1103 108-109 0.00608948101939788 1103 110-111 0.03716468450002708 1103 112-113 0.10648670503481128 1103 114-115 0.03945806984000555 1103 116-117 0.13617660395370734 1103 118-119 0.07740244816050179 1103 120-121 0.15297912779629286 1103 122-123 0.1134053029857327 1103 124-125 -0.0785869571576967 1104 1 0.12460116059594384 1104 2 0.06250969949470431 1104 3 0.07539292650961471 1104 4 -0.13605369678949586 1104 5 -0.31717649043067553 1104 6 -0.011287479757875474 1104 7 -0.13991916282742523 1104 8 0.01898212515400388 1104 9 0.11815855803869368 1104 10-11 0.11820785002164769 1104 12-13 0.18365499512166394 1104 14-15 0.203237979550984 1104 16-17 0.2021518898388308 1104 18-19 0.19024650621314976 1104 20-21 0.25597111662577277 1104 22-23 0.23683987573353704 1104 24-25 0.19858655972913652 1104 26-27 0.2534213628878845 1104 28-29 0.3559238906907325 1104 30-31 0.18188000851674246 1104 32-33 0.1025026354827645 1104 34-35 0.09462578385348053 1104 36-37 0.2016086772722474 1104 38-39 0.38234473104088096 1104 40-41 0.25086904765766604 1104 42-43 0.34164126583717547 1104 44-45 0.48754478655968825 1104 46-47 0.47350746507576247 1104 48-49 0.37467374294774203 1104 50-51 0.11714942945731366 1104 52-53 0.014872459620811185 1104 54-55 0.039623518570010674 1104 56-57 -0.09006910203046914 1104 58-59 -0.1423119922643039 1104 60-61 -0.019245868152474088 1104 62-63 0.029107502954474285 1104 64-65 0.0366080130048303 1104 66-67 -0.029870121714864695 1104 68-69 0.019074641881104526 1104 70-71 0.08182279311158425 1104 72-73 0.0551905225640823 1104 74-75 0.07622861106887058 1104 76-77 0.1668559195240249 1104 78-79 0.14952291582693888 1104 80-81 0.14075726032591263 1104 82-83 0.12799887439678415 1104 84-85 -0.012614340915103384 1104 86-87 0.15933397310775277 1104 88-89 0.12081240813229854 1104 90-91 0.07154516224261798 1104 92-93 0.15925083167913812 1104 94-95 0.2415458496689169 1104 96-97 0.37116620452114546 1104 98-99 0.40839971366521866 1104 100-101 0.3885450684655929 1104 102-103 0.5059912714284955 1104 104-105 0.4015413760511102 1104 106-107 0.45257835174286143 1104 108-109 0.5812462208313107 1104 110-111 0.4664328301474825 1104 112-113 0.4144442647165114 1104 114-115 0.3240252268209538 1104 116-117 0.2592173563048057 1104 118-119 0.45627874427817616 1104 120-121 0.5016166971235165 1104 122-123 0.5713206634873025 1104 124-125 0.6637086782413846 1105 1 -0.03695833511530111 1105 2 -0.12428438259613728 1105 3 -0.07261812639465148 1105 4 -0.2039513422906154 1105 5 -0.44788859681487736 1105 6 -0.019571401236198938 1105 7 -0.1462918529431363 1105 8 -0.16776878152954566 1105 9 -0.3199163944683576 1105 10-11 0.00820698651350682 1105 12-13 -0.013292503583073767 1105 14-15 -0.036394165978926196 1105 16-17 0.05845837766336359 1105 18-19 0.08421490181500957 1105 20-21 0.11537615951334601 1105 22-23 0.16310203680659185 1105 24-25 0.1917749196393359 1105 26-27 0.22049723647029396 1105 28-29 0.17778359940065513 1105 30-31 -0.09679709700395023 1105 32-33 0.04977538892239863 1105 34-35 0.16372082731673032 1105 36-37 0.20456619267147857 1105 38-39 0.29380924085608484 1105 40-41 0.18203017827766388 1105 42-43 0.13447846576810463 1105 44-45 0.17162025717163232 1105 46-47 0.004359171943534079 1105 48-49 -0.2682123891114472 1105 50-51 -0.2531789914741296 1105 52-53 -0.12916933417344012 1105 54-55 -0.06406797875310843 1105 56-57 -0.3560209758431512 1105 58-59 -0.334692972058221 1105 60-61 -0.08400034400303014 1105 62-63 -0.21283308768509812 1105 64-65 -0.4018843017726681 1105 66-67 -0.3040411538949357 1105 68-69 -0.4379773413092707 1105 70-71 -0.5465995775061145 1105 72-73 -0.400449624807969 1105 74-75 -0.4353179320201903 1105 76-77 -0.33859281687572107 1105 78-79 -0.30507094750850783 1105 80-81 -0.3065370808173711 1105 82-83 -0.31003865942395237 1105 84-85 -0.5257051244048299 1105 86-87 -0.4891879887827564 1105 88-89 -0.3626859512022236 1105 90-91 -0.426763800971937 1105 92-93 -0.39379591289515403 1105 94-95 -0.4651463384607233 1105 96-97 -0.30172539642295604 1105 98-99 -0.29763620827364434 1105 100-101 -0.3665640213968224 1105 102-103 -0.3473317957524351 1105 104-105 -0.3947066810555562 1105 106-107 -0.2573764579975055 1105 108-109 -0.22712117310110358 1105 110-111 -0.2230942552246873 1105 112-113 -0.1796953933342671 1105 114-115 -0.2954080239993786 1105 116-117 -0.23698320785384652 1105 118-119 -0.11182902153841567 1105 120-121 -0.2214648756126536 1105 122-123 -0.33307747133510546 1105 124-125 -0.318497297234984 1106 1 0.13199788885297892 1106 2 0.06185064335723922 1106 3 0.010640661004138963 1106 4 -0.06869462888317202 1106 5 -0.08929606204418405 1106 6 0.09983409256176401 1106 7 0.0650472959227244 1106 8 -0.14629385460355593 1106 9 -0.02190264003121456 1106 10-11 -0.12508684222267163 1106 12-13 -0.03633147068979525 1106 14-15 -0.1149978683953563 1106 16-17 -0.12463403460509426 1106 18-19 -0.11823001491195839 1106 20-21 0.10101348452032255 1106 22-23 -0.056092924078164685 1106 24-25 0.04900141202995201 1106 26-27 0.07183080151327914 1106 28-29 -0.06232078390030438 1106 30-31 0.045355352755890976 1106 32-33 0.23493467031858017 1106 34-35 0.09550844832329375 1106 36-37 0.09599787345913313 1106 38-39 0.13147383543066127 1106 40-41 0.15329343273474905 1106 42-43 0.16950698314916934 1106 44-45 0.2518763153345489 1106 46-47 -0.12929646572333553 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-0.4364382352117211 2216 96-97 -0.5471140784143103 2216 98-99 -0.7514588186071052 2216 100-101 -0.42977942313476447 2216 102-103 -0.6032262352028006 2216 104-105 -0.6490333365925629 2216 106-107 -0.5676029037832215 2216 108-109 -0.6819058569759378 2216 110-111 -0.6758306534953107 2216 112-113 -0.7787697052215989 2216 114-115 -0.8992948439129087 2216 116-117 -0.9247791070020028 2216 118-119 -0.876501980743928 2216 120-121 -1.009899660082496 2216 122-123 -1.1970259324455021 2216 124-125 -1.3884354420061804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 12.0 5 55.0 6 152.0 7 290.0 8 494.0 9 947.0 10 1768.0 11 2328.0 12 2718.0 13 4397.0 14 7863.0 15 8263.0 16 3145.0 17 2291.0 18 2210.0 19 1936.0 20 1729.0 21 1777.0 22 1779.0 23 1740.0 24 1978.0 25 2403.0 26 2761.0 27 3297.0 28 3964.0 29 4607.0 30 5659.0 31 7075.0 32 8944.0 33 11591.0 34 17481.0 35 36584.0 36 58832.0 37 131173.0 38 72319.0 39 331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.83943509852989 18.099412244032035 13.898090154725914 19.163062502712165 2 16.2345080912233 21.91258490120368 40.36645504634919 21.486451961223832 3 18.24842852184644 30.559947553121745 30.816879410743898 20.37474451428792 4 11.379770315677245 18.14607459795595 37.48736639739103 32.986788688975786 5 11.06536929154545 40.51734319299861 32.24091262964311 16.176374885812834 6 27.214059279437823 38.5680363535552 17.99790267950773 16.22000168749925 7 25.201131833541414 37.87207581549378 20.43301791748413 16.493774433480677 8 27.78270531354392 32.6853088199141 19.322153183161326 20.209832683380654 9 30.09529961292064 14.121889024396122 19.442663568746354 36.340147793936886 10-11 24.62545372160173 28.127967568245033 25.470231524552783 21.776347185600457 12-13 27.836814476671602 26.39514873391789 27.3360938245658 18.431942964844712 14-15 23.987110209157827 24.870692356267263 29.236301584470546 21.90589585010436 16-17 19.49954447074249 29.40983653971299 28.895980255578422 22.194638733966094 18-19 19.711763259757724 24.28127606037088 36.38256744966281 19.62439323020858 20-21 21.6270348178606 22.975787053328254 36.844242736839064 18.55293539197208 22-23 20.80443092585719 23.75573026883457 36.5822531585772 18.857585646731035 24-25 19.958375361684848 23.620475851322674 37.19018024890425 19.23096853808823 26-27 19.903013241681165 23.900342731536604 36.858462962338095 19.338181064444136 28-29 19.551287845099463 23.844506547987038 36.99471075455804 19.609494852355454 30-31 19.75047665418889 23.604445719271773 37.107799773905626 19.53727785263371 32-33 19.49809834611547 23.806465141165866 36.94523043995932 19.75020607275935 34-35 19.892408327749685 23.80007809072986 36.85376305730028 19.45375052422018 36-37 20.27900732587048 23.401209208495068 36.4573830530044 19.862400412630045 38-39 20.073294416512816 23.48723312975112 36.36256272565569 20.076909728080366 40-41 20.35878351996375 23.53869106439593 35.920169100173055 20.182356315467267 42-43 20.36938843389523 23.67101146776829 35.60527546263937 20.354324635697104 44-45 20.203092667959396 23.812892503817327 35.217512343796884 20.76650248442639 46-47 20.977196420264598 24.271435148026832 33.72283322607625 21.028535205632323 48-49 21.06509488987761 24.784527430573966 32.688201069168144 21.462176610380283 50-51 21.220436619124232 25.089872677862342 32.18219177256759 21.50749893044584 52-53 21.422649203908392 25.22957228453948 31.978756429229072 21.369022082323056 54-55 20.77750541708545 25.310382727029506 32.02094043977816 21.89117141610688 56-57 20.96425296124502 25.36361039909473 31.865997110157473 21.806139529502786 58-59 21.156349122181194 25.026994977006062 32.01893504815711 21.79772085265563 60-61 21.22175839113815 24.987466919899155 32.34101064829767 21.449764040665027 62-63 21.430250032840387 24.695247242914007 32.52114137515049 21.353361349095113 64-65 21.235475170706664 24.634062276297744 32.64950240182408 21.48096015117151 66-67 21.004116634771584 25.173655465628027 32.28388872553037 21.53833917407002 68-69 21.19877367744921 24.917269022099354 32.301957825880514 21.58199947457092 70-71 21.041049533730707 24.99596277558576 32.11148042006416 21.85150727061937 72-73 21.416855772788242 25.250156366559366 31.711258753815763 21.621729106836625 74-75 20.705491899291882 25.3445391923876 31.798352382008282 22.15161652631224 76-77 20.801744053623143 25.20984199635812 31.90393725648989 22.084476693528853 78-79 20.823019578258805 25.199144852120636 31.74857013393621 22.229265435684347 80-81 20.596167751265366 25.650156664256446 31.511328030850805 22.242347553627383 82-83 20.725384854554264 25.45680012147593 31.25920409161792 22.558610932351886 84-85 20.655962130816434 25.009640830846802 31.4552593142477 22.879137724089063 86-87 20.536503005009756 24.927420608570213 31.245473203170476 23.290603183249555 88-89 20.58370532486189 25.09074825734422 31.36479112233781 22.960755295456078 90-91 20.67107914501403 25.208130465383093 31.23499580605663 22.885794583546247 92-93 20.45249119627074 25.108284062176867 31.115307762948895 23.323916978603503 94-95 20.369691915369522 24.786206331377773 31.371268252001755 23.472833501250946 96-97 20.433827924644724 24.691724031545863 31.44475135458244 23.429696689226972 98-99 20.167854551765195 24.9464306804569 31.080810734592646 23.80490403318526 100-101 20.277450712186162 24.86667976268094 31.145656200701644 23.710213324431255 102-103 20.41456521870123 24.563832800062666 31.103117072494683 23.918484908741423 104-105 20.21258390677159 24.442328781980983 31.34347244483544 24.001614866411984 106-107 20.28021074856231 24.375997223440717 31.189774934803882 24.15401709319309 108-109 20.228432177192214 24.494933973531563 30.876428002405564 24.400205846870655 110-111 19.869098779685366 24.824713841882122 30.97871017346641 24.3274772049661 112-113 19.956855254198477 24.68024079106735 30.78618645038053 24.576717504353642 114-115 19.51304163392801 24.76837903890557 30.819326009515503 24.899253317650917 116-117 19.490553168105162 24.700832246728737 31.03234265523583 24.77627192993027 118-119 19.327906290107798 24.776239914677724 30.858826577648696 25.037027217565782 120-121 19.310774510997213 24.926578782141743 30.74580531958867 25.016841387272372 122-123 19.35388627511473 25.02952547144345 30.33856677336007 25.278021480081758 124-125 19.013134497495464 25.018716206848744 30.137011073002252 25.831138222653543 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1755.0 1 1141.5 2 1073.5 3 1437.0 4 737.5 5 442.0 6 1265.5 7 3000.0 8 5051.0 9 5436.0 10 3299.0 11 1319.0 12 887.5 13 850.0 14 929.5 15 1013.0 16 1095.5 17 1195.5 18 1275.5 19 1368.5 20 1463.0 21 1564.5 22 1699.5 23 1838.0 24 2052.5 25 2315.5 26 2731.0 27 3311.0 28 3737.5 29 4220.5 30 4864.5 31 5451.0 32 6219.0 33 7166.5 34 8248.0 35 8774.5 36 8983.0 37 9902.0 38 10395.5 39 10342.5 40 10508.5 41 10904.5 42 11173.5 43 11753.5 44 12579.0 45 13015.0 46 13522.5 47 13987.0 48 14082.5 49 13696.0 50 13271.0 51 12901.5 52 12655.5 53 12545.0 54 12083.0 55 11366.5 56 10543.5 57 9583.5 58 9214.0 59 8858.5 60 7866.0 61 6757.5 62 5774.5 63 4552.5 64 3349.0 65 2527.5 66 1753.5 67 1333.0 68 1020.0 69 648.0 70 418.0 71 367.0 72 341.5 73 325.0 74 239.5 75 144.5 76 117.0 77 72.5 78 41.5 79 20.0 80 8.5 81 1.0 82 1.5 83 2.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025066160201686182 2 0.0019281661693604756 3 0.0014461246270203567 4 0.0802599167996298 5 0.0031332700252107728 6 0.02096880709179517 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.43572699095208E-4 26-27 0.0 28-29 8.43572699095208E-4 30-31 0.007471643906271842 32-33 0.0 34-35 0.0 36-37 6.025519279251486E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.00421786349547604 46-47 0.002651228482870654 48-49 0.0 50-51 0.0022896973261155645 52-53 0.0 54-55 0.002651228482870654 56-57 1.2051038558502972E-4 58-59 0.0024102077117005944 60-61 0.0 62-63 0.003976842724305981 64-65 0.002169186940530535 66-67 0.0 68-69 7.230623135101783E-4 70-71 0.0031332700252107728 72-73 0.0027717388684556834 74-75 0.0 76-77 0.0015666350126053864 78-79 7.230623135101783E-4 80-81 4.820415423401189E-4 82-83 0.0012051038558502972 84-85 0.0 86-87 0.0027717388684556834 88-89 0.00433837388106107 90-91 0.00433837388106107 92-93 0.005061436194571249 94-95 0.003856332338720951 96-97 0.003374290796380832 98-99 0.004579394652231129 100-101 0.00421786349547604 102-103 0.0022896973261155645 104-105 0.001687145398190416 106-107 0.0 108-109 0.007351133520686813 110-111 0.002169186940530535 112-113 0.004699905037816159 114-115 0.0 116-117 2.4102077117005944E-4 118-119 0.0010845934702652675 120-121 3.6153115675508917E-4 122-123 0.0014461246270203567 124-125 0.037237709145774185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 414902.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.32884567716827 #Duplication Level Percentage of deduplicated Percentage of total 1 66.55618570366309 28.17246513511308 2 14.886125515643894 12.602250193652944 3 6.562269733426364 8.333199085144702 4 3.6054650587046746 6.104606962573303 5 2.19060830885522 4.636296052232758 6 1.418970787851294 3.603803729962038 7 1.0141897306166092 3.005063641624751 8 0.7222880040026586 2.4458893964718746 9 0.5327111465683462 2.0294143122239006 >10 2.3852089237856715 16.85928671776853 >50 0.08265464976432672 2.373791285904623 >100 0.03762211644445188 2.8817025134741567 >500 0.00114006413468036 0.3054706005240126 >1k 0.0034201924040410804 3.011032878308968 >5k 0.00114006413468036 3.6357274950203964 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8410 2.0269846855402 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6658 1.6047162944502555 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4512 1.0874857195193082 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1802 0.43431942964844716 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1736 0.41841205875122317 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.4147967471836723 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1403 0.3381521419515934 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1305 0.3145321063769276 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 651 0.15690452203170868 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 615 0.14822777426958655 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 460 0.11086955473822735 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 453 0.10918240934003692 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2051038558502972E-4 0.0 14-15 0.0 0.0 0.0 2.4102077117005944E-4 0.0 16-17 0.0 0.0 0.0 7.230623135101783E-4 0.0 18-19 0.0 0.0 0.0 8.43572699095208E-4 0.0 20-21 0.0 0.0 0.0 0.0015666350126053864 0.0 22-23 0.0 0.0 0.0 0.003856332338720951 0.0 24-25 0.0 0.0 0.0 0.008074195834196992 0.0 26-27 0.0 0.0 0.0 0.010845934702652674 0.0 28-29 0.0 0.0 0.0 0.015184308583713745 0.0 30-31 0.0 0.0 0.0 0.028681471769237073 0.0 32-33 0.0 0.0 0.0 0.04880670616193704 0.0 34-35 0.0 0.0 0.0 0.07170367942309269 0.0 36-37 0.0 0.0 0.0 0.10689271201392136 0.0 38-39 0.0 0.0 0.0 0.18028353683520448 0.0 40-41 0.0 0.0 0.0 0.2709073467951468 0.0 42-43 0.0 0.0 0.0 0.3517698155227017 0.0 44-45 0.0 0.0 0.0 0.42853493114036567 0.0 46-47 0.0 0.0 0.0 0.5226535422822739 0.0 48-49 0.0 0.0 0.0 0.6407537201556031 0.0 50-51 0.0 0.0 0.0 0.7830764855315231 0.0 52-53 0.0 0.0 0.0 0.9191327108570218 0.0 54-55 0.0 0.0 0.0 1.0434994287807724 0.0 56-57 0.0 0.0 0.0 1.1891964849530732 0.0 58-59 0.0 0.0 0.0 1.3615263363396657 0.0 60-61 0.0 0.0 0.0 1.5742271668972432 0.0 62-63 0.0 0.0 0.0 1.7883741220818412 0.0 64-65 0.0 0.0 0.0 1.9681756173747051 0.0 66-67 0.0 0.0 0.0 2.1711151066998955 0.0 68-69 0.0 0.0 0.0 2.433707236889675 0.0 70-71 0.0 0.0 0.0 2.731849930827039 0.0 72-73 0.0 0.0 0.0 3.0465025475895513 0.0 74-75 0.0 0.0 0.0 3.3305455264134665 0.0 76-77 0.0 0.0 0.0 3.621939638758068 0.0 78-79 0.0 0.0 0.0 3.9578020833835463 0.0 80-81 0.0 0.0 0.0 4.360306771237545 0.0 82-83 0.0 0.0 0.0 4.804508052503964 0.0 84-85 0.0 0.0 0.0 5.220509903543487 0.0 86-87 0.0 0.0 0.0 5.64012706615056 0.0 88-89 0.0 0.0 0.0 6.098548572916014 0.0 90-91 0.0 0.0 0.0 6.640483776891893 0.0 92-93 0.0 0.0 0.0 7.245204891757572 0.0 94-95 0.0 0.0 0.0 7.823895763336885 0.0 96-97 0.0 0.0 0.0 8.378123026642436 0.0 98-99 0.0 0.0 0.0 8.981036485724339 0.0 100-101 0.0 0.0 0.0 9.672404567825655 0.0 102-103 0.0 0.0 0.0 10.395828412492587 0.0 104-105 0.0 0.0 0.0 11.145041479674719 0.0 106-107 0.0 0.0 0.0 11.865452564702025 0.0 108-109 0.0 0.0 0.0 12.58887640936896 0.0 110-111 0.0 0.0 0.0 13.400031814741794 0.0 112-113 0.0 0.0 0.0 14.284698555321498 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1945 0.0 98.65574 1 GTATCAA 4580 0.0 68.43942 1 GTGGTAT 430 0.0 58.205887 1 TGGTATC 420 0.0 56.733498 2 GTGTACG 45 3.1445472E-4 52.97044 1 TATCAAC 6170 0.0 50.687744 2 ATCAACG 6170 0.0 50.591194 3 TCAACGC 6270 0.0 49.778316 4 ATAAGAC 60 2.175718E-5 49.641808 3 CAACGCA 6360 0.0 49.014763 5 AACGCAG 6485 0.0 48.16173 6 ACGCAGA 7395 0.0 42.154686 7 CGCAGAG 7420 0.0 42.012657 8 GCAGAGT 7910 0.0 39.10926 9 GTACATG 4310 0.0 38.16084 1 TACATGG 4415 0.0 37.239788 2 ACATGGG 4400 0.0 36.419037 3 CATGGGA 3270 0.0 34.42631 4 ATGGGAG 1195 0.0 33.3549 5 ATATGGG 110 2.0202911E-5 32.49282 3 >>END_MODULE