FastQCFastQC Report
Fri 27 May 2016
SRR940141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275911
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG27550.12105042771883436No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG22960.10088267950723907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.060.2247241
GTATCAA42550.057.0668071
ATCAACG49200.048.1438643
TCAACGC49400.048.0694274
CAACGCA51950.045.5943455
TATCAAC53000.045.2534982
AACGCAG53200.044.404356
GTACATG119800.038.1528851
TACATGG121950.035.967372
ACGCAGA66100.035.372927
ACATGGG123550.034.6826863
CGCAGAG67550.034.1762478
CATGGGG63500.029.616684
CATGGGA71900.028.143174
GCAGAGT80150.028.1354399
TGGACCG2901.4551915E-1024.6261585
GAGTACA90450.023.5557771
ATGGGAT31750.022.8680735
AGAGTAC71500.022.59341210-11
AGTACAT90200.022.2354722
Produced by FastQC (version 0.11.5)