FastQCFastQC Report
Fri 27 May 2016
SRR940141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275911
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50210.22061495374819137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38300.16828426067627425No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG25400.11160366112734636No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG25220.11081276904061714No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG23230.10206901763733292No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC23050.10127812555060368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37200.078.633321
GTATCAA75200.058.70411
ATCAACG93700.046.849013
TCAACGC94300.046.54994
TATCAAC96100.045.741992
CAACGCA98600.044.537145
AACGCAG99400.044.177726
ACGCAGA117300.037.275827
CGCAGAG119300.036.6010488
GTACATG109800.035.9732171
TACATGG112300.034.3698272
GCAGAGT131250.032.906019
ACATGGG113950.032.721453
GTGGTAT16850.028.9922581
GAGTACT73900.028.21509212-13
CAGAGTA128850.027.35532210-11
CATGGGG60050.027.0777534
TGGTATC17000.026.6285042
CATGGGA65250.026.4716174
GTACTTT79250.026.19775414-15
Produced by FastQC (version 0.11.5)