FastQCFastQC Report
Fri 27 May 2016
SRR940140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494704
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44790.9053898897118277No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9430.1906190368381901No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6690.13523238138361524No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6670.1348280992270125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.12694459717325918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.10187910346388952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.074.960091
GTATCAA24450.059.8773421
ATCAACG30300.045.7635353
TCAACGC30600.045.3148734
TATCAAC32550.044.9769862
CAACGCA31400.044.160355
AACGCAG32150.042.5705456
GTACATG65250.042.0461961
GTCGGGA2550.042.0086362
TACATGG66500.040.8083882
CGTCACT600.001306468639.674821
ATGGGGA10500.038.5412565
CATGGGG21800.038.4918564
ACATGGG69600.036.8531233
GTGGTAT4250.036.4074861
ATGGGAC6150.035.8041085
GGATAGG1001.05947165E-535.707341
ATGGGCG700.00278464934.0069925
ACGCAGA40100.033.530397
CGGGAGT3200.033.4756364
Produced by FastQC (version 0.11.5)