##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940140_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 494704 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.797743701284 33.0 31.0 34.0 22.0 34.0 2 29.840278226980175 33.0 31.0 34.0 22.0 34.0 3 30.023347294543807 33.0 31.0 34.0 23.0 34.0 4 32.574106940716064 37.0 35.0 37.0 18.0 37.0 5 33.180871794042496 37.0 35.0 37.0 25.0 37.0 6 32.8617072026909 37.0 35.0 37.0 18.0 37.0 7 32.92110635854976 37.0 35.0 37.0 17.0 37.0 8 32.638941670170446 37.0 35.0 37.0 16.0 37.0 9 33.71443529868366 39.0 35.0 39.0 13.0 39.0 10-11 33.94636995051586 39.0 35.0 39.0 13.5 39.0 12-13 33.836240458941106 39.0 35.0 39.0 12.5 39.0 14-15 35.13646139913969 40.0 36.0 41.0 13.0 41.0 16-17 35.37341925676768 40.0 36.0 41.0 17.0 41.0 18-19 35.523396819107994 40.0 36.0 41.0 18.0 41.0 20-21 35.7320074226204 40.0 36.0 41.0 22.0 41.0 22-23 35.92990555968822 40.0 36.0 41.0 24.5 41.0 24-25 36.15675939551732 40.0 36.0 41.0 27.0 41.0 26-27 36.25820288495747 40.0 36.0 41.0 28.0 41.0 28-29 36.21381573627866 40.0 35.0 41.0 28.5 41.0 30-31 36.12623306057763 40.0 35.0 41.0 28.5 41.0 32-33 36.042174714576795 40.0 35.0 41.0 29.0 41.0 34-35 35.91887775316149 40.0 35.0 41.0 28.0 41.0 36-37 35.75004346033184 40.0 35.0 41.0 27.0 41.0 38-39 35.564703337753485 40.0 35.0 41.0 27.0 41.0 40-41 35.43416972573499 40.0 35.0 41.0 26.5 41.0 42-43 35.35196198130599 40.0 34.5 41.0 26.0 41.0 44-45 35.406401605808725 40.0 35.0 41.0 26.0 41.0 46-47 35.31027139461173 40.0 34.5 41.0 26.0 41.0 48-49 35.17646713994631 40.0 34.0 41.0 25.5 41.0 50-51 35.05009763414082 40.0 34.0 41.0 25.5 41.0 52-53 34.87779156829134 40.0 34.0 41.0 25.0 41.0 54-55 34.691505425466545 39.5 33.5 41.0 25.0 41.0 56-57 34.47434526504738 39.0 33.0 41.0 24.5 41.0 58-59 34.216202011708006 39.0 33.0 41.0 22.5 41.0 60-61 33.95344185614024 38.5 33.0 41.0 21.0 41.0 62-63 33.644030571816685 38.0 33.0 41.0 16.0 41.0 64-65 33.33218449820499 37.0 33.0 40.5 8.5 41.0 66-67 32.98451397199133 37.0 32.0 40.0 2.0 41.0 68-69 32.638426210420775 36.0 32.0 40.0 2.0 41.0 70-71 32.49386703968433 36.0 32.0 39.0 2.0 41.0 72-73 32.222574509201465 35.5 32.0 39.0 2.0 41.0 74-75 31.87301396390569 35.0 32.0 39.0 2.0 41.0 76-77 31.502792578996733 35.0 32.0 37.5 2.0 40.0 78-79 31.13872436851127 35.0 32.0 37.0 2.0 39.0 80-81 30.779398387722757 35.0 32.0 36.5 2.0 39.0 82-83 30.430949618357644 35.0 31.0 36.0 2.0 38.0 84-85 30.10465652187975 35.0 31.0 36.0 2.0 37.0 86-87 29.83446060674666 35.0 31.0 35.5 2.0 37.0 88-89 29.595003274685467 35.0 31.0 35.0 2.0 36.5 90-91 29.38157261716097 35.0 30.0 35.0 2.0 36.0 92-93 29.176056793557358 35.0 30.0 35.0 2.0 36.0 94-95 29.002464099744493 35.0 30.0 35.0 2.0 36.0 96-97 28.828459038131893 35.0 30.0 35.0 2.0 35.5 98-99 28.695674585206508 35.0 30.0 35.0 2.0 35.0 100-101 28.582324986254406 35.0 29.5 35.0 2.0 35.0 102-103 27.850898719234127 34.0 27.5 35.0 2.0 35.0 104-105 28.30261530127106 35.0 29.0 35.0 2.0 35.0 106-107 28.30002688476341 35.0 29.0 35.0 2.0 35.0 108-109 28.215628941751028 35.0 29.0 35.0 2.0 35.0 110-111 28.07387548918141 35.0 28.0 35.0 2.0 35.0 112-113 27.902331495197128 35.0 28.0 35.0 2.0 35.0 114-115 27.712250153627217 35.0 27.0 35.0 2.0 35.0 116-117 27.569794260810504 35.0 27.0 35.0 2.0 35.0 118-119 27.376954704227174 35.0 27.0 35.0 2.0 35.0 120-121 27.1480390293994 35.0 26.0 35.0 2.0 35.0 122-123 26.849906206539668 35.0 25.0 35.0 2.0 35.0 124-125 24.953353924771175 32.0 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30445562618410094 1101 2 0.2931243099995662 1101 3 0.3250617044010333 1101 4 0.46577121954732803 1101 5 0.47096358540249383 1101 6 0.08663821381523462 1101 7 0.3472548194069418 1101 8 0.38942812582406106 1101 9 0.30198136034138656 1101 10-11 0.4277478319111978 1101 12-13 0.34509375232431694 1101 14-15 0.45723309099263076 1101 16-17 0.20095356806568532 1101 18-19 0.3884606123928407 1101 20-21 0.3581551650154182 1101 22-23 0.24722043410733363 1101 24-25 0.2660086242748747 1101 26-27 0.5541367077698851 1101 28-29 0.45887588946798985 1101 30-31 0.36319775035582325 1101 32-33 0.34502787117380507 1101 34-35 0.5088815902810779 1101 36-37 0.4440500121312425 1101 38-39 0.4233941880862204 1101 40-41 0.44668321115170784 1101 42-43 0.3715408609035933 1101 44-45 0.2984290987724023 1101 46-47 0.14202164153542896 1101 48-49 0.25302499099871767 1101 50-51 0.2430377533862611 1101 52-53 0.2689236124079315 1101 54-55 0.25812562680904705 1101 56-57 0.31535062496450905 1101 58-59 0.345784097451336 1101 60-61 0.11539520616620536 1101 62-63 0.11341303913448542 1101 64-65 0.0712667238742668 1101 66-67 0.03241152073443487 1101 68-69 0.03149320647025178 1101 70-71 0.12445225677578264 1101 72-73 -0.06217855803165406 1101 74-75 -0.03402962873326132 1101 76-77 -0.11551896852075316 1101 78-79 0.0809099011749943 1101 80-81 0.07189096200248457 1101 82-83 0.09744803389512668 1101 84-85 0.09740137950483074 1101 86-87 0.12712257495072166 1101 88-89 0.13037159696280654 1101 90-91 -0.042346110211564536 1101 92-93 0.16554072511515727 1101 94-95 0.1478995299219079 1101 96-97 0.2316440084378577 1101 98-99 0.29213378021840697 1101 100-101 0.12616131473547654 1101 102-103 0.15683030151182464 1101 104-105 -0.06113749946511504 1101 106-107 0.14848178831675796 1101 108-109 0.18164005531745886 1101 110-111 0.23261218467381184 1101 112-113 0.33927576325941544 1101 114-115 0.312515281432006 1101 116-117 0.30470579418542343 1101 118-119 0.2588635358408915 1101 120-121 0.3082703362645809 1101 122-123 0.3197533689094776 1101 124-125 0.34836397842511246 1102 1 0.6834149022022018 1102 2 0.7366386388080137 1102 3 0.6277766365277309 1102 4 0.6223323055201817 1102 5 0.7204733893240629 1102 6 0.5396925124577692 1102 7 0.5894493895426933 1102 8 0.5617509010880113 1102 9 0.6962498369628065 1102 10-11 0.8216957957121096 1102 12-13 0.7186608714796705 1102 14-15 0.6172632569051473 1102 16-17 0.726349495667499 1102 18-19 0.9209266757412564 1102 20-21 0.6830797502341284 1102 22-23 0.5860590464753557 1102 24-25 0.6235312487092699 1102 26-27 0.7702189098814927 1102 28-29 0.9018811685026833 1102 30-31 0.9391027277313952 1102 32-33 0.691992426226598 1102 34-35 0.7513778195420144 1102 36-37 0.729268714997005 1102 38-39 0.7666634188554582 1102 40-41 0.6677616425242618 1102 42-43 0.60146167538322 1102 44-45 0.6131538348206718 1102 46-47 0.8840465284132577 1102 48-49 0.8628666199579982 1102 50-51 0.7904547971268343 1102 52-53 0.9698474434788196 1102 54-55 1.063970272359576 1102 56-57 1.010090443969034 1102 58-59 0.8899394519008084 1102 60-61 1.0137549346729955 1102 62-63 0.938488453915781 1102 64-65 0.8407802985349022 1102 66-67 0.8373511889093876 1102 68-69 0.6634502200449148 1102 70-71 0.7884982597923766 1102 72-73 0.8117844887255146 1102 74-75 1.0450804768474278 1102 76-77 1.161837743394777 1102 78-79 1.1902990414464902 1102 80-81 1.1165176588350647 1102 82-83 1.1419616085557607 1102 84-85 0.9493998712092058 1102 86-87 0.9666022129597707 1102 88-89 1.0968874340668826 1102 90-91 1.0937964237250881 1102 92-93 0.9418604837878561 1102 94-95 1.1065345223804286 1102 96-97 1.0397322437319758 1102 98-99 1.2333668118926155 1102 100-101 1.041545930120094 1102 102-103 1.1071131069416893 1102 104-105 1.1037757735363947 1102 106-107 1.1232743976681903 1102 108-109 1.0735706737186632 1102 110-111 1.1505043415365535 1102 112-113 1.1473639985535335 1102 114-115 1.0109692784154163 1102 116-117 0.942978795693719 1102 118-119 0.8456282417232437 1102 120-121 0.9908118143942914 1102 122-123 1.1271251637812725 1102 124-125 0.9628247627388333 1103 1 -0.09446358005261146 1103 2 0.1694354368599491 1103 3 -0.1443571474770664 1103 4 -0.07676953169292489 1103 5 -0.3125410212594488 1103 6 0.057137859456624085 1103 7 -0.32999535777236133 1103 8 -0.09214522640839817 1103 9 -0.17787335262813997 1103 10-11 -0.013029985239754183 1103 12-13 -0.05477336319658832 1103 14-15 -0.23628214642764078 1103 16-17 -0.16236145673941849 1103 18-19 -0.007455404616372618 1103 20-21 -0.11276085199379082 1103 22-23 -0.07140111089621826 1103 24-25 -0.014014409034828645 1103 26-27 0.03289290904557163 1103 28-29 -0.06071128275029736 1103 30-31 0.03454785666340854 1103 32-33 0.17498889172660625 1103 34-35 0.069556643434872 1103 36-37 0.022903662024937432 1103 38-39 0.0025401838339149663 1103 40-41 0.0613359397130111 1103 42-43 0.08561775672215788 1103 44-45 -0.030008179753465924 1103 46-47 0.03579378894860952 1103 48-49 0.1865561745564719 1103 50-51 0.03914866763147984 1103 52-53 0.14772410850999051 1103 54-55 0.06051754743272397 1103 56-57 -0.06219189806879655 1103 58-59 -0.1411134929101152 1103 60-61 -0.34993966272110555 1103 62-63 -0.2002031904898942 1103 64-65 -0.39595510461616357 1103 66-67 -0.3175353254739335 1103 68-69 -0.2926297559677309 1103 70-71 -0.2676721230966521 1103 72-73 -0.43828592869076033 1103 74-75 -0.002252520299528271 1103 76-77 -0.17729253336838013 1103 78-79 0.015831942280595257 1103 80-81 -0.07384787570127571 1103 82-83 -0.0614640851693693 1103 84-85 0.01253249219087138 1103 86-87 -0.13552802745891768 1103 88-89 -0.01940692890537221 1103 90-91 0.13671129588340492 1103 92-93 0.1562565295092 1103 94-95 0.04156536975181524 1103 96-97 -0.28237973358907453 1103 98-99 -0.09184921056826667 1103 100-101 -0.226744425874017 1103 102-103 -0.3687632491614572 1103 104-105 -0.2575939133559686 1103 106-107 -0.2448917056591462 1103 108-109 -0.31321288585901286 1103 110-111 -0.2881177940646751 1103 112-113 -0.18781495254497926 1103 114-115 0.024926691779278087 1103 116-117 -0.16173644536099374 1103 118-119 0.057623280702692625 1103 120-121 0.12235609104842382 1103 122-123 0.36286109392719723 1103 124-125 0.789779641075711 1104 1 0.2221742157658575 1104 2 0.16664530638094988 1104 3 0.1526894078429244 1104 4 0.3672102500951695 1104 5 0.06356856730941729 1104 6 0.30366043042720037 1104 7 -0.08781418673628139 1104 8 -0.04444799973345681 1104 9 0.22981103298297256 1104 10-11 0.49796894610795306 1104 12-13 0.46662661439618347 1104 14-15 0.48328711775354805 1104 16-17 0.4799308886076332 1104 18-19 0.40798640554271515 1104 20-21 0.41873961338704646 1104 22-23 0.47028279209773416 1104 24-25 0.3690371103474206 1104 26-27 0.421805856974629 1104 28-29 0.4929971130647246 1104 30-31 0.46917477635935967 1104 32-33 0.5268243451281975 1104 34-35 0.6258364838264754 1104 36-37 0.6613857017719056 1104 38-39 0.6155878681333462 1104 40-41 0.6799218714226853 1104 42-43 0.6619294412284233 1104 44-45 0.5571526955072343 1104 46-47 0.500450699854035 1104 48-49 0.5817151365848474 1104 50-51 0.5548014073456997 1104 52-53 0.5298814934144076 1104 54-55 0.646897401613387 1104 56-57 0.7034565325636066 1104 58-59 0.728580950097971 1104 60-61 0.6421445539739992 1104 62-63 0.6927301307780098 1104 64-65 0.7006900597322172 1104 66-67 0.8832635782956473 1104 68-69 0.8764176784049553 1104 70-71 0.8919362675474716 1104 72-73 0.6770444917032634 1104 74-75 0.8498759506658793 1104 76-77 0.5000778925412632 1104 78-79 0.6223998447919286 1104 80-81 0.5864779770660178 1104 82-83 0.5951979707797506 1104 84-85 0.7478579140499804 1104 86-87 0.8037677823899188 1104 88-89 0.7772873589757694 1104 90-91 0.6121613794795913 1104 92-93 0.6806631689424698 1104 94-95 0.7931974344914607 1104 96-97 0.8276225071334729 1104 98-99 0.8643198864205459 1104 100-101 0.7880227975260183 1104 102-103 0.8433814670424127 1104 104-105 0.9454338629854497 1104 106-107 0.9957457368146088 1104 108-109 1.1784716634972092 1104 110-111 1.1285822679861042 1104 112-113 1.2387550614164482 1104 114-115 1.267246830704078 1104 116-117 1.1845909242030999 1104 118-119 1.0080451398129533 1104 120-121 0.9892873353388865 1104 122-123 0.9554966576412802 1104 124-125 0.9135106164794777 1105 1 0.07038348729989252 1105 2 0.08219392065185005 1105 3 0.18022772496398431 1105 4 -0.016817689941966307 1105 5 -0.07215541004669035 1105 6 0.07612498273483936 1105 7 0.050916541975631446 1105 8 0.15838986524762078 1105 9 0.3901997995795483 1105 10-11 0.31377724367590787 1105 12-13 0.41620870260241816 1105 14-15 0.4684163035326634 1105 16-17 0.4109590459015209 1105 18-19 0.1800963784471179 1105 20-21 0.3838883522731322 1105 22-23 0.3605947809807475 1105 24-25 0.41284201379114194 1105 26-27 0.21584031471408593 1105 28-29 0.10653938179902411 1105 30-31 0.146330229708596 1105 32-33 0.214682767516301 1105 34-35 0.26710255775115854 1105 36-37 -0.046410126078775704 1105 38-39 0.07794711240781282 1105 40-41 0.10782850021289647 1105 42-43 0.07035469875964395 1105 44-45 0.12091140114893761 1105 46-47 0.11414156400298481 1105 48-49 0.0771148275063851 1105 50-51 0.08794125920119455 1105 52-53 0.2024458608488544 1105 54-55 0.1789540441358639 1105 56-57 0.15000215033110464 1105 58-59 0.22493882524502595 1105 60-61 0.2526339555341437 1105 62-63 0.4210188035032729 1105 64-65 0.48156716210113615 1105 66-67 0.3458575008456748 1105 68-69 0.33279313905073593 1105 70-71 0.3767508409659044 1105 72-73 0.26072933005195154 1105 74-75 0.176142291037511 1105 76-77 0.21930531936058628 1105 78-79 0.2352626738026693 1105 80-81 0.2940319530693962 1105 82-83 0.08091129025784483 1105 84-85 0.1323541858422672 1105 86-87 0.28536039730029117 1105 88-89 0.24562526289910025 1105 90-91 0.24901365719109947 1105 92-93 0.4691729516447367 1105 94-95 0.42546989904376886 1105 96-97 0.34434205327183776 1105 98-99 0.44322302680529546 1105 100-101 0.49703608298088753 1105 102-103 0.4095966086077283 1105 104-105 0.39212602657567786 1105 106-107 0.4482310719843774 1105 108-109 0.6991438476653435 1105 110-111 0.7829134656446541 1105 112-113 0.5011479189985018 1105 114-115 0.8718149611892976 1105 116-117 0.8526031479693472 1105 118-119 0.8302481641907988 1105 120-121 0.9221713981219892 1105 122-123 0.8413128666340697 1105 124-125 0.8975401961901532 1106 1 0.20477313955308318 1106 2 0.33530345438459364 1106 3 0.34997814825129936 1106 4 0.28376921419973655 1106 5 0.06452976187308224 1106 6 0.4216983742430429 1106 7 0.41904626325721495 1106 8 0.25540406165293916 1106 9 0.3963496758494074 1106 10-11 0.4011351982213611 1106 12-13 0.22722109189651007 1106 14-15 0.46112680757016733 1106 16-17 0.28207823384109076 1106 18-19 0.3061912273660994 1106 20-21 0.3528744163523214 1106 22-23 0.629815688118029 1106 24-25 0.5751747339005462 1106 26-27 0.42581117301051563 1106 28-29 0.21705603652681305 1106 30-31 0.44463992950021236 1106 32-33 0.37820801823263395 1106 34-35 0.33674756621691415 1106 36-37 0.21920876881573292 1106 38-39 0.10900581910226492 1106 40-41 0.21700406676668393 1106 42-43 0.2941678662511862 1106 44-45 0.1508772939595744 1106 46-47 0.16056275116109475 1106 48-49 0.19613996426075175 1106 50-51 0.36210526675524335 1106 52-53 0.28875984235445173 1106 54-55 0.2841450118357898 1106 56-57 0.5032516944832253 1106 58-59 0.4284545519967864 1106 60-61 0.27267294680792276 1106 62-63 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8 -0.5706559077893871 2216 9 -0.5881446900787779 2216 10-11 -0.7508235966602328 2216 12-13 -0.7003894409529963 2216 14-15 -0.8133551443014895 2216 16-17 -0.8404329865561664 2216 18-19 -0.9496696397080058 2216 20-21 -0.9908994568333185 2216 22-23 -1.069376204548135 2216 24-25 -1.0586762496747042 2216 26-27 -0.9985943846671006 2216 28-29 -1.2027837743975596 2216 30-31 -1.1012980479635033 2216 32-33 -1.2139007002547615 2216 34-35 -1.2226310147609354 2216 36-37 -1.2419793996334647 2216 38-39 -1.333853711073445 2216 40-41 -1.306152923302072 2216 42-43 -1.2329759458190992 2216 44-45 -1.277831405429275 2216 46-47 -1.3247850811536495 2216 48-49 -1.4121010594214454 2216 50-51 -1.3866891373700412 2216 52-53 -1.3964125220458499 2216 54-55 -1.3825256336951526 2216 56-57 -1.5447334086489377 2216 58-59 -1.531211700867992 2216 60-61 -1.3933232812287528 2216 62-63 -1.5204104902772784 2216 64-65 -1.5017374778064045 2216 66-67 -1.4411178910302773 2216 68-69 -1.2870992305045377 2216 70-71 -1.312732617173797 2216 72-73 -1.3767793983677876 2216 74-75 -1.223315343018978 2216 76-77 -1.0642584643190744 2216 78-79 -1.1884178299174408 2216 80-81 -1.1911342480815499 2216 82-83 -1.0545477644241998 2216 84-85 -0.9840061835203784 2216 86-87 -1.0290749040408755 2216 88-89 -0.9182628568187035 2216 90-91 -0.977430143825007 2216 92-93 -1.1482197790865243 2216 94-95 -1.0114492095738896 2216 96-97 -1.0716122940831525 2216 98-99 -0.9858073962521772 2216 100-101 -1.0386496096342697 2216 102-103 -0.9378125556310337 2216 104-105 -1.1125030456835994 2216 106-107 -1.183556026809292 2216 108-109 -1.0899986001447282 2216 110-111 -1.0289424371749298 2216 112-113 -1.0937074300178935 2216 114-115 -1.0448376597444629 2216 116-117 -1.0259664747221393 2216 118-119 -1.0225440271843205 2216 120-121 -0.9059103360043252 2216 122-123 -1.0474735218468219 2216 124-125 -1.1707536686337114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14036.0 3 2909.0 4 2657.0 5 2048.0 6 2985.0 7 2889.0 8 2510.0 9 2525.0 10 3106.0 11 3705.0 12 3788.0 13 3674.0 14 3467.0 15 3409.0 16 3228.0 17 3044.0 18 2734.0 19 2371.0 20 2131.0 21 1985.0 22 2061.0 23 3120.0 24 5476.0 25 5923.0 26 8519.0 27 6172.0 28 5213.0 29 7369.0 30 7506.0 31 7668.0 32 10053.0 33 13451.0 34 19660.0 35 31890.0 36 62242.0 37 139465.0 38 89147.0 39 568.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.908036658370754 21.04601300793941 12.458225590906977 19.587724742782857 2 14.443833467433803 24.80076983103341 39.65761926518334 21.09777743634945 3 18.177977237350152 34.21816564578406 27.871510299795823 19.732346817069963 4 10.621295972403919 20.78643165784743 36.77852973230913 31.813742637439518 5 11.061281269461272 46.29780901466318 29.842570950235757 12.798338765639786 6 26.065859147070064 39.980711725208806 19.62774034019341 14.325688787527724 7 26.13568593995435 39.54234954726204 19.672419474504554 14.649545038279049 8 26.562860065251403 37.17247959377565 19.59988033199785 16.6647800089751 9 29.18262057923316 20.21635324893764 17.82498473680593 32.77604143502327 10-11 22.93391603868172 33.41715450046897 24.882353892428604 18.766575568420713 12-13 24.426862129539973 31.624717751961334 25.514616253848377 18.433803864650315 14-15 22.101583688223574 31.101424985822923 24.72714811786029 22.069843208093214 16-17 20.40509604354176 34.59942692251325 24.855290354206357 20.14018667973863 18-19 20.47403990191302 32.81334969464059 27.65316993589575 19.059440467550637 20-21 23.571226424629565 31.104833329964222 26.325880854676665 18.998059390729548 22-23 22.658714396459278 31.797154445660663 26.23551778193517 19.308613375944887 24-25 20.879689573648935 31.890869652670595 27.227196873903953 20.00224389977652 26-27 20.789283245266848 32.04242149819487 26.95430784593553 20.213987410602748 28-29 20.736885621209012 31.878308348991713 26.977226530874216 20.407579498925056 30-31 20.54388298409952 32.16886125384575 26.999587630533128 20.287668131521603 32-33 20.306751566606025 32.17525773195876 26.799878714372348 20.71811198706287 34-35 20.726030110935024 31.930406869562404 27.15886671625861 20.18469630324396 36-37 20.870285054692253 31.755974100271487 26.525995750762622 20.847745094273638 38-39 20.35902422064192 32.05778428225477 26.764900889828624 20.818290607274683 40-41 20.572552611016597 31.886941025322223 27.04154195588953 20.49896440777164 42-43 20.328133638852865 31.654626811663782 26.98905244134298 21.02818710814037 44-45 20.31281426716057 31.916960193247455 26.889292049261933 20.880933490330047 46-47 20.692272781028603 32.23544228819564 26.491502757952173 20.580782172823582 48-49 20.051365034379803 32.152874147579965 26.608644324931852 21.187116493108373 50-51 20.568988834913185 31.92905530387109 26.68380951225774 20.818146348957985 52-53 20.54881333695853 32.00609275994546 26.68725887457789 20.75783502851812 54-55 19.870908532246588 32.14500857105893 26.99853240830584 20.98555048838864 56-57 20.30734334899734 32.28338338469874 26.27347958237855 21.135793683925375 58-59 20.176719022617043 31.835201070705804 26.59589953641835 21.3921803702588 60-61 20.007762311551694 32.23734480430485 26.509405738450532 21.24548714569293 62-63 19.958194689836134 32.14005587518244 26.418611044849737 21.483138390131682 64-65 19.502928557350703 32.27821042152022 26.58513536049808 21.633725660630994 66-67 19.491448335250325 32.36099122900996 26.195524955579046 21.952035480160664 68-69 19.803862511724184 31.86622707720172 26.492407581099 21.837502829975097 70-71 19.683292448606025 32.19523190080122 26.227881059303577 21.89359459128918 72-73 19.61194937978001 32.23304464189155 26.13322929795322 22.021776680375215 74-75 19.44026384760234 32.36347978842867 26.390925841883483 21.805330522085512 76-77 19.402301639045987 32.23477324761573 26.57619820174973 21.786726911588556 78-79 19.83828501255818 32.17107424234001 26.121619777742964 21.86902096735884 80-81 19.63191052165045 32.52140989448937 26.102939318501804 21.743740265358372 82-83 19.864564382454013 32.32858297958359 25.96715180917728 21.839700828785123 84-85 19.906348038422976 32.026120670138106 26.352829166531905 21.714702124907014 86-87 19.74606755427801 32.31301489695239 26.17220387771542 21.768713671054186 88-89 19.818512236981213 31.80058582260774 26.311682693571115 22.069219246839932 90-91 19.784426077894878 31.90591079678863 26.145918721111343 22.163744404205147 92-93 19.95303716896814 31.879332007807598 26.12168488001027 22.045945943213994 94-95 19.707845499531032 31.427985219444356 26.449957954655712 22.4142113263689 96-97 19.727735080917398 31.64116983557778 26.20941496092597 22.421680122578845 98-99 19.30880154601403 31.728865363564967 26.113951826542404 22.8483812638786 100-101 19.1125400239335 32.166810860635856 26.252567191694425 22.468081923736214 102-103 19.243243680305476 32.37141034983581 25.944934693205123 22.44041127665359 104-105 18.762388296856187 32.70328430743293 26.190059847918967 22.34426754779191 106-107 18.73099873863967 33.07816391215757 25.781982761408845 22.408854587793915 108-109 18.58313253986222 33.603528574662825 25.384472330929203 22.42886655454575 110-111 18.497334753719922 34.17660364543431 25.23867952561244 22.08738207523332 112-113 18.711951297661006 34.24355414678422 25.367182927901656 21.67731162765311 114-115 18.393241937505305 34.62031380761596 25.15625694868384 21.83018730619489 116-117 18.23184819982899 34.86455509669438 25.04987841189562 21.85371829158101 118-119 18.074118247562833 34.86084508750562 25.042426202665347 22.0226104622662 120-121 18.17583657099887 34.99094396985636 24.88629426437948 21.946925194765292 122-123 18.262637860215403 34.77988857983764 24.9700831204114 21.987390439535563 124-125 18.455986179049283 34.79503238779581 24.489300496437544 22.259680936717363 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3504.0 1 3036.0 2 2733.0 3 2516.0 4 1738.5 5 1145.5 6 882.0 7 807.5 8 913.5 9 986.5 10 777.5 11 579.0 12 555.0 13 566.5 14 557.0 15 596.0 16 672.5 17 735.5 18 840.5 19 973.0 20 1139.5 21 1373.0 22 1702.0 23 2205.5 24 2719.5 25 3374.0 26 4104.0 27 4892.0 28 5766.0 29 6837.0 30 8652.5 31 10032.5 32 11585.0 33 13054.5 34 13992.5 35 14565.0 36 15447.5 37 16985.5 38 17040.0 39 16644.5 40 16433.5 41 15978.0 42 15304.5 43 15574.0 44 16426.0 45 16459.0 46 15748.5 47 14955.0 48 14426.5 49 13958.5 50 13459.0 51 12660.5 52 11646.0 53 11242.5 54 11060.0 55 10588.0 56 10180.0 57 9744.5 58 9806.5 59 9469.5 60 8491.0 61 7300.5 62 5816.5 63 3715.0 64 2139.0 65 1626.0 66 1238.0 67 932.0 68 595.0 69 380.0 70 303.5 71 263.5 72 238.0 73 258.5 74 223.5 75 159.0 76 111.0 77 89.5 78 62.0 79 30.5 80 27.0 81 26.5 82 17.5 83 9.5 84 7.0 85 5.5 86 1.5 87 1.5 88 2.0 89 3.0 90 2.0 91 0.5 92 1.0 93 2.5 94 2.5 95 0.5 96 0.5 97 1.5 98 3.5 99 5.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01677770949901355 2 0.010107053915068406 3 0.006872796662246516 4 0.0285018920404929 5 0.02668262233578059 6 0.020820531065040913 7 0.0095006306801643 8 4.042821566027362E-4 9 0.010107053915068406 10-11 0.0 12-13 0.0030321161745205214 14-15 0.013644522785342346 16-17 0.0013139170089588926 18-19 0.008287784210356091 20-21 0.002829975096219153 22-23 5.053526957534202E-4 24-25 0.005963161809890359 26-27 4.042821566027362E-4 28-29 0.005558879653287623 30-31 4.042821566027362E-4 32-33 8.085643132054724E-4 34-35 0.0 36-37 0.005457809114136939 38-39 4.042821566027362E-4 40-41 0.012027394158931401 42-43 0.005558879653287623 44-45 3.032116174520521E-4 46-47 0.008793136906109512 48-49 0.0019203402438629968 50-51 0.007479219897150619 52-53 0.0037396099485753095 54-55 0.0032342572528218897 56-57 0.009197419062712249 58-59 0.015564863029205343 60-61 0.0012128464698082085 62-63 0.006064232349041043 64-65 0.0013139170089588926 66-67 0.0010107053915068404 68-69 0.0 70-71 0.004143892105178046 72-73 1.0107053915068405E-4 74-75 0.004952456418383518 76-77 0.011420970924027298 78-79 0.0013139170089588926 80-81 0.002931045635369837 82-83 8.085643132054724E-4 84-85 0.0 86-87 0.0011117759306575245 88-89 0.0030321161745205214 90-91 0.004952456418383518 92-93 0.011623112002328666 94-95 0.0 96-97 4.042821566027362E-4 98-99 0.0025267634787671015 100-101 0.0 102-103 1.0107053915068405E-4 104-105 0.006771726123095831 106-107 0.0 108-109 0.0 110-111 6.064232349041042E-4 112-113 0.005154597496684886 114-115 0.0012128464698082085 116-117 2.021410783013681E-4 118-119 0.004346033183479414 120-121 0.0012128464698082085 122-123 0.0 124-125 0.07721789191112262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 494704.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.31639862488131 #Duplication Level Percentage of deduplicated Percentage of total 1 73.3210639508944 36.159308174027224 2 12.011710177042778 11.847485745151705 3 5.1361435143352665 7.598883028426704 4 2.760342389389827 5.445205824652242 5 1.718365134268465 4.237178997234066 6 1.096308658696781 3.243959689691964 7 0.805611242922259 2.781089163484815 8 0.5983018637088569 2.3604874566940315 9 0.4096444505687484 1.8181970116846993 >10 2.013310280891896 16.655363678924402 >50 0.08531191796757956 2.83606848824503 >100 0.0414254986039585 3.420839472987495 >500 0.0020507672576217076 0.6899528998557442 >1k 4.101534515243416E-4 0.9059803689398646 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4479 0.9053898897118277 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 943 0.1906190368381901 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 669 0.13523238138361524 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 667 0.1348280992270125 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 628 0.12694459717325918 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 504 0.10187910346388952 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.0107053915068405E-4 0.0 16-17 0.0 0.0 0.0 3.0321161745205217E-4 0.0 18-19 0.0 0.0 0.0 5.053526957534202E-4 0.0 20-21 0.0 0.0 0.0 0.0018192697047123127 0.0 22-23 0.0 0.0 0.0 0.005963161809890359 0.0 24-25 0.0 0.0 0.0 0.011319900384876614 0.0 26-27 0.0 0.0 0.0 0.01728306219476697 0.0 28-29 0.0 0.0 0.0 0.02749118664898606 0.0 30-31 0.0 0.0 0.0 0.05862091270739675 0.0 32-33 0.0 0.0 0.0 0.09874591675021832 0.0 34-35 0.0 0.0 0.0 0.158983958084026 0.0 36-37 0.0 0.0 0.0 0.24560141013616224 0.0 38-39 0.0 0.0 0.0 0.39326546783531163 0.0 40-41 0.0 0.0 0.0 0.5865123386914195 0.0 42-43 0.0 0.0 0.0 0.7432727449141305 0.0 44-45 0.0 0.0 0.0 0.9043791843203208 0.0 46-47 0.0 0.0 0.0 1.0966153497849218 0.0 48-49 0.0 0.0 0.0 1.3254390504220706 0.0 50-51 0.0 0.0 0.0 1.6064151492609722 0.0 52-53 0.0 0.0 0.0 1.8558572398848603 0.0 54-55 0.0 0.0 0.0 2.084074517287105 0.0 56-57 0.0 0.0 0.0 2.346049354765678 0.0 58-59 0.0 0.0 0.0 2.648351337365374 0.0 60-61 0.0 0.0 0.0 3.0217059089880007 0.0 62-63 0.0 0.0 0.0 3.392331576053559 0.0 64-65 0.0 0.0 0.0 3.7259654257899673 0.0 66-67 0.0 0.0 0.0 4.086585109479608 0.0 68-69 0.0 0.0 0.0 4.527758012872344 0.0 70-71 0.0 0.0 0.0 5.024317571719655 0.0 72-73 0.0 0.0 0.0 5.533713089039102 0.0 74-75 0.0 0.0 0.0 6.002882531776578 0.0 76-77 0.0 0.0 0.0 6.4759937255409294 0.0 78-79 0.0 0.0 0.0 7.020662861023966 0.0 80-81 0.0 0.0 0.0 7.6663014651185355 0.0 82-83 0.0 0.0 0.0 8.353277919725734 0.0 84-85 0.0 0.0 0.0 9.006800025874057 0.0 86-87 0.0 0.0 0.0 9.61292004916071 0.0 88-89 0.0 0.0 0.0 10.291507649018403 0.0 90-91 0.0 0.0 0.0 11.081879265176752 0.0 92-93 0.0 0.0 0.0 11.935217827225978 0.0 94-95 0.0 0.0 0.0 12.761974837478572 0.0 96-97 0.0 0.0 0.0 13.545979009670429 0.0 98-99 0.0 0.0 0.0 14.354037970180148 0.0 100-101 0.0 0.0 0.0 15.266603868171675 0.0 102-103 0.0 0.0 0.0 16.238397102105502 0.0 104-105 0.0 0.0 0.0 17.247485364985934 0.0 106-107 0.0 0.0 0.0 18.188755296096254 0.0 108-109 0.0 0.0 0.0 19.130732720980625 0.0 110-111 0.0 0.0 0.0 20.17933956466897 0.0 112-113 0.0 0.0 0.0 21.279391312785016 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1175 0.0 74.96009 1 GTATCAA 2445 0.0 59.877342 1 ATCAACG 3030 0.0 45.763535 3 TCAACGC 3060 0.0 45.314873 4 TATCAAC 3255 0.0 44.976986 2 CAACGCA 3140 0.0 44.16035 5 AACGCAG 3215 0.0 42.570545 6 GTACATG 6525 0.0 42.046196 1 GTCGGGA 255 0.0 42.008636 2 TACATGG 6650 0.0 40.808388 2 CGTCACT 60 0.0013064686 39.67482 1 ATGGGGA 1050 0.0 38.541256 5 CATGGGG 2180 0.0 38.491856 4 ACATGGG 6960 0.0 36.853123 3 GTGGTAT 425 0.0 36.407486 1 ATGGGAC 615 0.0 35.804108 5 GGATAGG 100 1.05947165E-5 35.70734 1 ATGGGCG 70 0.002784649 34.006992 5 ACGCAGA 4010 0.0 33.53039 7 CGGGAGT 320 0.0 33.475636 4 >>END_MODULE