FastQCFastQC Report
Fri 27 May 2016
SRR940139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483723
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19660.4064309532521712No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT12950.2677152006416896No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT10420.21541253982134403No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC8700.1798549996588957No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC8590.17758097092757633No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT8370.17303291346493757No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT8060.16662428704031026No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA8020.16579736750164867No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7190.1486387870744207No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7020.14512437903510894No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA6870.14202343076512797No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT6790.1403695916878048No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT6760.1397494020338086No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6180.12775906872321557No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA5960.12321101126057682No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5960.12321101126057682No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT5880.12155717218325364No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA5870.12135044229858824No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT5730.11845622391327268No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG5620.1161821951819533No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5570.11514854575862632No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC5510.11390816645063394No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG5450.11266778714264156No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA5450.11266778714264156No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT5320.10998029864199138No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5290.1093601089879952No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA5250.10853318944933361No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5210.10770626991067202No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG4980.10295148256336789No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG4860.1004707239473831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA603.943569E-969.457342
GGTATCA5650.068.491431
GTATGGT1950.067.167541
GTACATT350.007775512551.029881
GTATCAA11400.048.045681
CATATAG2150.047.0740743
ACATATA2300.044.004032
CTCCTCG1551.8189894E-1242.2287039
GTACATG21750.041.8796961
AGGTTAG905.128608E-639.6899071
TGGGTAT1202.0667358E-839.6775936
GTGGTAC3300.039.6775936
CATGGGT3350.039.0975234
GGTACTC3350.039.077318
TGGTACT3400.038.498667
TCAACGC12400.038.4095884
ATGGGTA2200.037.8858225
CAACGCA12800.036.744175
GGTATGG650.001937137836.61798
ACATGGG21650.036.573383
Produced by FastQC (version 0.11.5)