##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940139_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 483723 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.403408562338363 33.0 31.0 34.0 28.0 34.0 2 31.43908393853507 34.0 31.0 34.0 28.0 34.0 3 31.59268424284146 34.0 31.0 34.0 28.0 34.0 4 34.730457307177865 37.0 35.0 37.0 32.0 37.0 5 34.90093503926834 37.0 35.0 37.0 32.0 37.0 6 34.79526092412393 37.0 36.0 37.0 32.0 37.0 7 34.70400001653839 37.0 35.0 37.0 32.0 37.0 8 34.62423535783909 37.0 35.0 37.0 32.0 37.0 9 36.14737773477796 39.0 38.0 39.0 32.0 39.0 10-11 36.23727112417644 39.0 38.0 39.0 32.0 39.0 12-13 36.25063414392121 39.0 38.0 39.0 32.0 39.0 14-15 37.721411841074335 41.0 39.0 41.0 32.0 41.0 16-17 37.790534045311055 41.0 39.0 41.0 32.0 41.0 18-19 37.896837652954275 41.0 39.0 41.0 32.0 41.0 20-21 37.99738796790725 41.0 39.0 41.0 32.0 41.0 22-23 38.01403179092166 41.0 39.0 41.0 32.0 41.0 24-25 38.07858836565555 41.0 39.0 41.0 32.0 41.0 26-27 38.05707502020785 41.0 39.0 41.0 32.0 41.0 28-29 37.96573452161671 41.0 39.0 41.0 32.0 41.0 30-31 37.87207244642079 41.0 38.0 41.0 31.0 41.0 32-33 37.7729888799995 41.0 38.0 41.0 31.0 41.0 34-35 37.68160186718432 40.5 38.0 41.0 31.0 41.0 36-37 37.522658629008745 40.0 38.0 41.0 30.0 41.0 38-39 37.385097462804126 40.0 38.0 41.0 30.0 41.0 40-41 37.310725353146324 40.0 38.0 41.0 30.0 41.0 42-43 37.26631460567308 40.0 38.0 41.0 30.0 41.0 44-45 37.342149329264885 40.0 38.0 41.0 30.0 41.0 46-47 37.31008552415329 40.0 38.0 41.0 30.0 41.0 48-49 37.22791866419418 40.0 38.0 41.0 30.0 41.0 50-51 37.15192062399348 40.0 38.0 41.0 30.0 41.0 52-53 37.087445707564044 40.0 38.0 41.0 30.0 41.0 54-55 36.98212303322356 40.0 38.0 41.0 30.0 41.0 56-57 36.846913419457 40.0 38.0 41.0 30.0 41.0 58-59 36.66592967462783 40.0 37.5 41.0 30.0 41.0 60-61 36.50104501956699 40.0 37.0 41.0 30.0 41.0 62-63 36.23278405202977 40.0 36.5 41.0 30.0 41.0 64-65 35.968189438997115 39.0 36.0 41.0 29.5 41.0 66-67 35.63571300103572 39.0 36.0 41.0 29.0 41.0 68-69 35.27226946000087 38.5 35.0 40.5 29.0 41.0 70-71 35.11581421598725 37.5 35.0 40.0 29.0 41.0 72-73 34.801116548107075 37.0 35.0 39.5 29.0 41.0 74-75 34.37842525577655 37.0 35.0 39.0 29.0 41.0 76-77 33.98337064807751 36.0 35.0 39.0 28.5 41.0 78-79 33.55996613764489 36.0 35.0 37.5 28.0 39.5 80-81 33.15348040097329 35.5 35.0 37.0 27.5 39.0 82-83 32.76409846130947 35.0 34.0 37.0 26.5 39.0 84-85 32.3917696698317 35.0 34.0 36.0 26.0 38.0 86-87 32.12750479096508 35.0 34.0 36.0 26.0 37.0 88-89 31.867301120682704 35.0 34.0 36.0 25.0 37.0 90-91 31.656733295708495 35.0 34.0 35.5 24.0 36.5 92-93 31.462111580387948 35.0 34.0 35.0 23.0 36.0 94-95 31.287506486150132 35.0 34.0 35.0 20.0 36.0 96-97 31.113185645503727 35.0 34.0 35.0 18.5 36.0 98-99 30.98693467128915 35.0 34.0 35.0 16.5 36.0 100-101 30.906159103453838 35.0 34.0 35.0 12.5 35.5 102-103 30.148972655838158 34.5 31.5 35.0 8.0 35.0 104-105 30.653179608991096 35.0 33.0 35.0 7.0 35.0 106-107 30.63764695910676 35.0 33.0 35.0 3.5 35.0 108-109 30.54881822861431 35.0 33.0 35.0 2.0 35.0 110-111 30.412657657378293 35.0 33.0 35.0 2.0 35.0 112-113 30.260181963644484 35.0 33.0 35.0 2.0 35.0 114-115 30.08247488748726 35.0 33.0 35.0 2.0 35.0 116-117 29.925234069911912 35.0 33.0 35.0 2.0 35.0 118-119 29.713002069366148 35.0 32.5 35.0 2.0 35.0 120-121 29.45389303382308 35.0 32.0 35.0 2.0 35.0 122-123 29.14503135058701 35.0 32.0 35.0 2.0 35.0 124-125 27.096975955247114 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17869839001954801 1101 2 0.2308349724640415 1101 3 0.28048151106368735 1101 4 0.2738849800086953 1101 5 0.19592574025332254 1101 6 0.18189656727042802 1101 7 0.3790572428739907 1101 8 0.40625048883803316 1101 9 0.49243693931705934 1101 10-11 0.380198065838556 1101 12-13 0.4423034048833969 1101 14-15 0.2618345505342603 1101 16-17 0.29789912108836347 1101 18-19 0.3716581307528841 1101 20-21 0.38969372729770413 1101 22-23 0.2893716633950447 1101 24-25 0.2787910418155519 1101 26-27 0.542236085858562 1101 28-29 0.35987997766956425 1101 30-31 0.37488501468869373 1101 32-33 0.4632546989671411 1101 34-35 0.4225751639487072 1101 36-37 0.5518616202868785 1101 38-39 0.5554884253022081 1101 40-41 0.6829598082503239 1101 42-43 0.46606762016475756 1101 44-45 0.5063669997611129 1101 46-47 0.435373725116186 1101 48-49 0.4167406473999975 1101 50-51 0.5118352376720878 1101 52-53 0.5767164065276447 1101 54-55 0.5471384564970023 1101 56-57 0.439724177003022 1101 58-59 0.3165673799174016 1101 60-61 0.4803298744410114 1101 62-63 0.31851576146162586 1101 64-65 0.3508874061512941 1101 66-67 0.5496369335893405 1101 68-69 0.4107901203818187 1101 70-71 0.28334389552012595 1101 72-73 0.34829250378797383 1101 74-75 0.39742283133470835 1101 76-77 0.34799036613914325 1101 78-79 0.5006773972047185 1101 80-81 0.5462885239504871 1101 82-83 0.48550412694079625 1101 84-85 0.4976722442830308 1101 86-87 0.42613129650958825 1101 88-89 0.32324316948493603 1101 90-91 0.26135165803015425 1101 92-93 0.26938092553939086 1101 94-95 0.40660991243028377 1101 96-97 0.36404366091780815 1101 98-99 0.27879153734643225 1101 100-101 0.37320787475327677 1101 102-103 0.34697128547540856 1101 104-105 0.31485800512482953 1101 106-107 0.4083455066818189 1101 108-109 0.4563186782322681 1101 110-111 0.35092045964991314 1101 112-113 0.5264833139847944 1101 114-115 0.6222748747348135 1101 116-117 0.5300345107851605 1101 118-119 0.715768616182288 1101 120-121 0.6181965254604442 1101 122-123 0.45233821414104014 1101 124-125 0.6724845965986788 1102 1 0.39168479362141895 1102 2 0.32776153850768 1102 3 0.39944161563417424 1102 4 0.34921595231315905 1102 5 0.37445974242069724 1102 6 0.3501567422249039 1102 7 0.26894094171991156 1102 8 0.24735250092620475 1102 9 0.2986195578247006 1102 10-11 0.29728349858833525 1102 12-13 0.367801399498461 1102 14-15 0.4984800275254031 1102 16-17 0.21384835524358436 1102 18-19 0.3552731862221634 1102 20-21 0.518276327930046 1102 22-23 0.3577066911576168 1102 24-25 0.33265154746302983 1102 26-27 0.3244937359272768 1102 28-29 0.554073857614604 1102 30-31 0.5792130710852632 1102 32-33 0.35919588923851364 1102 34-35 0.40562938196029563 1102 36-37 0.4195475513105791 1102 38-39 0.3230782766020752 1102 40-41 0.39345931109360777 1102 42-43 0.2907143037384756 1102 44-45 0.29457265505834584 1102 46-47 0.37640982204499096 1102 48-49 0.2784461742185371 1102 50-51 0.20447508394452996 1102 52-53 0.2816008315575331 1102 54-55 0.3895207867654662 1102 56-57 0.30860551854409835 1102 58-59 0.15169122221259101 1102 60-61 0.16369914457027335 1102 62-63 0.12104735046384718 1102 64-65 0.0014543882424717935 1102 66-67 0.12875970336668274 1102 68-69 -0.02360306912984811 1102 70-71 0.048535105342601526 1102 72-73 -0.11195953790615931 1102 74-75 0.07401066049155247 1102 76-77 0.08338457230836127 1102 78-79 0.15683376136922078 1102 80-81 0.22723680848054073 1102 82-83 0.32719267222674375 1102 84-85 0.21096587955992874 1102 86-87 0.2512192764892518 1102 88-89 0.2927791937953437 1102 90-91 0.31472729623413187 1102 92-93 0.26720125645583437 1102 94-95 0.4002954636039071 1102 96-97 0.34588235870239004 1102 98-99 0.34076295921462574 1102 100-101 0.46188834263919176 1102 102-103 0.32545277895651026 1102 104-105 0.3671767663667147 1102 106-107 0.23835701390455455 1102 108-109 0.2655914887876314 1102 110-111 0.1799164265359252 1102 112-113 0.01434040098163436 1102 114-115 -0.0749633918082182 1102 116-117 -0.09403457723950481 1102 118-119 -0.24703065539934244 1102 120-121 -0.010582703443912322 1102 122-123 0.17953771363271542 1102 124-125 0.29902717890884034 1103 1 0.27500267737189077 1103 2 0.445313409051618 1103 3 0.3949669586721356 1103 4 0.23307857691118272 1103 5 0.3138104021751076 1103 6 0.35356480576274407 1103 7 0.27638649624550027 1103 8 0.4818451251809037 1103 9 0.40754026141014776 1103 10-11 0.455880905347243 1103 12-13 0.3668275035714288 1103 14-15 0.4547393974113021 1103 16-17 0.2228800669429276 1103 18-19 0.5736490345187022 1103 20-21 0.34709838306788043 1103 22-23 0.29832993877437275 1103 24-25 0.44033514000602736 1103 26-27 0.28257501217726144 1103 28-29 0.1692831136313515 1103 30-31 0.31210923844429317 1103 32-33 0.27238983300203046 1103 34-35 0.2977077513998836 1103 36-37 0.12038869526635665 1103 38-39 0.04543439520909942 1103 40-41 0.09843064353430009 1103 42-43 0.004545685846714775 1103 44-45 0.009888341534818323 1103 46-47 0.06562944644845459 1103 48-49 0.238762490122447 1103 50-51 0.11870128872588737 1103 52-53 0.09193145263075309 1103 54-55 0.19153495460516012 1103 56-57 0.20625718194918363 1103 58-59 0.11141473190004803 1103 60-61 0.07756142584423742 1103 62-63 0.25058123082686024 1103 64-65 0.27440960734045206 1103 66-67 0.06749581304727315 1103 68-69 0.11666083263368421 1103 70-71 0.05762747095983656 1103 72-73 -0.09635774804337416 1103 74-75 -0.11039784936757258 1103 76-77 -0.10471238240720027 1103 78-79 -0.019584453116770817 1103 80-81 -0.14600794724439226 1103 82-83 -0.10133641304350505 1103 84-85 -0.04771506938778458 1103 86-87 -0.0687438733945811 1103 88-89 -0.13873108876036255 1103 90-91 -0.050688526427546066 1103 92-93 -0.03564032237709469 1103 94-95 -0.02245891091686758 1103 96-97 -0.17652640292771338 1103 98-99 -0.2304596177757965 1103 100-101 -0.4198742831032476 1103 102-103 -0.38152127718841555 1103 104-105 -0.11728209768840259 1103 106-107 -0.13440136606362785 1103 108-109 -0.15124169004987564 1103 110-111 -0.13984873980458445 1103 112-113 -0.19064008676742006 1103 114-115 -0.20851187488806033 1103 116-117 -0.023256032704654928 1103 118-119 0.19442458086483683 1103 120-121 -0.069438747664055 1103 122-123 0.024903410981107044 1103 124-125 -0.06982646204285459 1104 1 -0.026706161933407913 1104 2 -0.0567171254010006 1104 3 0.028021799718843 1104 4 0.0029003817273007826 1104 5 -0.14798829908043132 1104 6 -0.09148447873141663 1104 7 -0.010101642907592634 1104 8 -0.15354931693723017 1104 9 0.06165815869368174 1104 10-11 0.0119183389643851 1104 12-13 -0.19022685892051072 1104 14-15 0.07366690301105194 1104 16-17 0.0030506185437602085 1104 18-19 0.062077314446653986 1104 20-21 0.1076515126166484 1104 22-23 0.21764693477976493 1104 24-25 0.07074131336248968 1104 26-27 0.0724728540282058 1104 28-29 0.17630191684564522 1104 30-31 0.11828126558898333 1104 32-33 0.047459123743983866 1104 34-35 0.035705664369373835 1104 36-37 0.08767748312701684 1104 38-39 -0.08866396108821561 1104 40-41 0.051273059438273094 1104 42-43 -0.14282259527761454 1104 44-45 -0.16488384645744958 1104 46-47 -0.31518050749064486 1104 48-49 -0.1761411674982014 1104 50-51 -0.1627420856169337 1104 52-53 -0.20812238478026046 1104 54-55 -0.3063148722510718 1104 56-57 -0.21171055548376927 1104 58-59 -0.11465586822092177 1104 60-61 -0.10030475163311792 1104 62-63 -0.2356270867208181 1104 64-65 -0.10485833680157697 1104 66-67 -0.05273442496725522 1104 68-69 0.10096490101384603 1104 70-71 0.15544539416509195 1104 72-73 0.020771853892114223 1104 74-75 0.11612139584521941 1104 76-77 -0.02874181352521532 1104 78-79 0.14117671482271987 1104 80-81 0.08747692843107302 1104 82-83 -0.003418790928961357 1104 84-85 -0.0683328038297688 1104 86-87 0.0866900494916365 1104 88-89 -0.012342781448449358 1104 90-91 0.10033870466788031 1104 92-93 0.031260969056109644 1104 94-95 0.046127292083404825 1104 96-97 0.08622010262551782 1104 98-99 0.13778827359227463 1104 100-101 0.11363452031044119 1104 102-103 0.3173747235560036 1104 104-105 0.24915792012447113 1104 106-107 0.07821320459963133 1104 108-109 0.15967932847418354 1104 110-111 0.31308340511775157 1104 112-113 0.28158712040026757 1104 114-115 0.33011480586938546 1104 116-117 0.3168036537090444 1104 118-119 0.1560272075987683 1104 120-121 0.11163441139558827 1104 122-123 0.03544823228742899 1104 124-125 0.2981921218338357 1105 1 0.20650286068689283 1105 2 0.12983606789684643 1105 3 0.15104690623293848 1105 4 -0.00515537747115502 1105 5 0.09506783198768431 1105 6 0.21132791834037334 1105 7 0.18028499528448094 1105 8 0.376324566125696 1105 9 0.19073305324207013 1105 10-11 0.15787292836716205 1105 12-13 -0.0068706821655553085 1105 14-15 0.040223071266140664 1105 16-17 0.12941830200831106 1105 18-19 0.16121138939433166 1105 20-21 0.2144949767418538 1105 22-23 0.11905897698166257 1105 24-25 0.15875329819020578 1105 26-27 0.2341309676928205 1105 28-29 0.14461224738465006 1105 30-31 0.13135620263676628 1105 32-33 0.18485858612671535 1105 34-35 0.08520789027488007 1105 36-37 0.005486852117336127 1105 38-39 0.10925356884729354 1105 40-41 0.18810834243107877 1105 42-43 0.16338869043514848 1105 44-45 0.14215511485748067 1105 46-47 -0.12109854741881776 1105 48-49 -0.11253126939048741 1105 50-51 -0.05043957496552309 1105 52-53 0.019968648911124376 1105 54-55 -0.11968836750707368 1105 56-57 -0.2624059517374491 1105 58-59 -0.3590326678044278 1105 60-61 -0.22245968315597509 1105 62-63 -0.29465980062555275 1105 64-65 -0.1920915203245599 1105 66-67 -0.19477907057547128 1105 68-69 -0.24752052700812044 1105 70-71 -0.20209877863742776 1105 72-73 -0.1278368223581623 1105 74-75 -0.056075503824345674 1105 76-77 -0.07325886109266122 1105 78-79 -0.05591526448007755 1105 80-81 -0.03728215315713612 1105 82-83 -0.09454598097559597 1105 84-85 -0.07241593263479018 1105 86-87 0.026824347561102968 1105 88-89 -0.15721257394517707 1105 90-91 -0.040461771325276175 1105 92-93 0.148102414460336 1105 94-95 0.025294091560663645 1105 96-97 0.08757052071325688 1105 98-99 -0.05451721201637483 1105 100-101 -0.1037392299419011 1105 102-103 -0.05563497692955721 1105 104-105 -0.025562380771201987 1105 106-107 -0.07367854945640318 1105 108-109 -0.21265888166404423 1105 110-111 -0.17159556512205398 1105 112-113 -0.12189251707994586 1105 114-115 -0.07517743055471726 1105 116-117 0.01471905867509804 1105 118-119 0.03458945326648433 1105 120-121 -0.1012089039817532 1105 122-123 -0.38435010811143044 1105 124-125 -0.4515682878602725 1106 1 0.23543547467187054 1106 2 0.2850460541445976 1106 3 0.3440967316851733 1106 4 0.24025877676559304 1106 5 0.2183302883507423 1106 6 0.23874778504935534 1106 7 0.21001100819043472 1106 8 0.14290660357412577 1106 9 0.13321692079734504 1106 10-11 0.4456598743102518 1106 12-13 0.46463042859294035 1106 14-15 0.3615126089790266 1106 16-17 0.5380610609208176 1106 18-19 0.3753233115354462 1106 20-21 0.4376780932128099 1106 22-23 0.46300798787767405 1106 24-25 0.3657669446516536 1106 26-27 0.3683370398392327 1106 28-29 0.38436153332561673 1106 30-31 0.42199938469536846 1106 32-33 0.24249108374652195 1106 34-35 0.3246715526043005 1106 36-37 0.26840338655085105 1106 38-39 0.22313276063825782 1106 40-41 0.3047538517931869 1106 42-43 0.2385077002732956 1106 44-45 0.16340233796125858 1106 46-47 0.19725541428645954 1106 48-49 0.05848322336313316 1106 50-51 0.1381407698985413 1106 52-53 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-0.8346286264317904 2216 100-101 -0.8168642245936653 2216 102-103 -0.8854781239161476 2216 104-105 -0.713652504935812 2216 106-107 -0.7737144895904073 2216 108-109 -0.8552222689361031 2216 110-111 -0.8575814800123922 2216 112-113 -0.7654998796731114 2216 114-115 -0.6213622141865542 2216 116-117 -0.6069716679146104 2216 118-119 -0.7058209488351608 2216 120-121 -0.6712416779610955 2216 122-123 -0.8899507789939278 2216 124-125 -1.3868863152603446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7253.0 3 983.0 4 885.0 5 1214.0 6 2538.0 7 2747.0 8 1985.0 9 1682.0 10 1757.0 11 1849.0 12 1948.0 13 2098.0 14 1994.0 15 2043.0 16 1721.0 17 1666.0 18 1435.0 19 1344.0 20 1066.0 21 1002.0 22 1128.0 23 1427.0 24 2218.0 25 2656.0 26 3920.0 27 3468.0 28 3416.0 29 4770.0 30 5711.0 31 6366.0 32 8559.0 33 12156.0 34 17414.0 35 26381.0 36 51112.0 37 182839.0 38 110863.0 39 109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.83176920834393 25.23358495793507 14.862392149808645 22.07225368391236 2 13.593379148369596 24.677888869592543 41.28067085121691 20.44806113082095 3 14.910750096134365 32.43139314200183 32.01046934244094 20.647387419422863 4 9.863773965691221 21.528800185274026 43.44882632214521 25.158599526889546 5 9.80023613532084 42.607951267624856 37.59969066686517 9.992121930189136 6 23.793267441379594 45.358959513667756 19.711343616889295 11.136429428063357 7 23.875465433367307 35.93300653932589 26.320838389957185 13.87068963734962 8 22.562846274704373 38.71971388406516 23.2361696849417 15.481270156288762 9 25.385949714375506 18.9949077586902 23.725130614025474 31.89401191290882 10-11 20.0339037361297 31.052101153037125 31.825271720873022 17.088723389960155 12-13 20.887351692856658 30.281636273048466 30.390792811380397 18.440219222714482 14-15 20.235478741990644 31.21866296704455 27.35328841131969 21.192569879645117 16-17 18.210469301391925 34.08146051736649 28.95513717674931 18.752933004492277 18-19 18.292811511505302 33.449833571088924 30.314147491161698 17.943207426244083 20-21 18.814959681712416 33.1723220460045 30.004434443115603 18.00828382916747 22-23 19.463802481182334 32.41765397822298 29.41478662286731 18.703756917727368 24-25 19.013137424552067 33.61667333409828 29.439243158646626 17.930946082703027 26-27 18.483175218772804 33.44334854182473 30.107954792121905 17.96552144728056 28-29 18.508380667869197 31.609305306464204 31.15334322233191 18.728970803334693 30-31 19.158500785578433 32.12674687835938 30.024911105598278 18.689841230463905 32-33 18.407484510635307 33.30280596792766 30.006574064694586 18.283135456742446 34-35 19.02258009499248 32.52949780090858 30.289267090118816 18.158655013980123 36-37 19.404276433101266 32.123279529044495 28.99281058926292 19.47963344859132 38-39 17.904657751738863 33.116231377417336 29.793548133684638 19.185562737159163 40-41 18.207258010327234 32.31101623515912 30.87430027549633 18.607425479017312 42-43 18.503874334292615 32.16731169991069 29.91238298435381 19.41643098144289 44-45 17.736020292773283 32.622245559922185 30.104254742939602 19.53747940436493 46-47 18.541796961818566 32.435972316264454 29.545306531728272 19.47692419018871 48-49 17.89797276903294 32.96069578304535 29.683966145284195 19.45736530263751 50-51 17.70006460783047 32.22700607313607 29.893371236593875 20.17955808243959 52-53 18.139719023829535 32.515068538462884 29.870179540692547 19.47503289701503 54-55 18.540539534806797 32.41333187241195 29.619112986419633 19.42701560636162 56-57 18.49007569985931 32.18458650386149 29.462885567407817 19.862452228871387 58-59 17.962032736246094 31.137064034479227 30.618510979451198 20.282392249823477 60-61 18.76806321038291 32.56866604095775 28.732443283069887 19.930827465589456 62-63 18.224773411398896 31.994682610565313 30.133906420561672 19.64663755747412 64-65 18.36437251985409 31.161641167915505 29.84930222558048 20.62468408664993 66-67 18.107639050398113 31.768476913742187 29.37824375127269 20.745640284587008 68-69 18.011153088806083 31.753329646646577 29.815648434794745 20.419868829752595 70-71 18.679819270570068 31.547332938465782 29.54994402597443 20.22290376498972 72-73 18.51538693712504 31.351685472234053 29.172024427253668 20.960903163387236 74-75 17.83916767021051 32.13865960998755 29.09712064750544 20.925052072296506 76-77 17.736194689076186 31.655892991718414 30.426215747105168 20.18169657210023 78-79 17.88700831271177 30.890375984304875 29.82952842261074 21.39308728037261 80-81 18.17556916556839 32.185647466211854 29.10261777398765 20.536165594232113 82-83 18.860640165054505 30.92642629295107 29.600036384760575 20.61289715723385 84-85 18.293443038105526 30.92982029986201 29.246003648786235 21.53073301324623 86-87 18.434764991477444 31.457165509136033 29.619828970068212 20.48824052931831 88-89 18.910143765014286 30.37837033239199 29.843127850388456 20.868358052205267 90-91 19.05646451740948 30.401672930854122 29.90281206197204 20.63905048976436 92-93 19.324175403846912 30.52375527487228 29.610822456224504 20.54124686505631 94-95 18.74502556215024 29.577154693905396 30.257916204108547 21.419903539835815 96-97 19.241896921879473 29.79881977298874 29.66062013084002 21.298663174291764 98-99 18.426196498547178 30.12599400264826 29.511167250862343 21.936642247942224 100-101 18.398959735220362 30.401283379124 29.825540650330872 21.37421623532476 102-103 19.070644842859284 29.967595057083347 29.35412349022425 21.60763660983312 104-105 18.259289351435502 30.961817163153114 29.299212940278974 21.479680545132407 106-107 18.58615364578488 30.620727151696318 29.193567392908754 21.599551809610045 108-109 18.257039669397567 31.4441322823186 28.381015581231406 21.917812467052425 110-111 18.02636638399742 31.910684235986785 28.345930100346894 21.7170192796689 112-113 17.642881508357366 31.487683608811157 29.49649062961929 21.372944253212182 114-115 17.786111435567882 32.22665435913126 28.528708566726863 21.458525638573995 116-117 17.662502777935696 32.44587547612526 28.217107949289105 21.67451379664994 118-119 17.817892007889157 31.210228611831255 28.7009357000798 22.270943680199792 120-121 17.53074117870817 31.890406767607438 28.44447981873661 22.13437223494778 122-123 17.885357823958987 31.70609181917318 28.625709988681525 21.782840368186303 124-125 17.982776901393883 31.877524632134268 27.813907781426984 22.32579068504487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1423.0 1 1050.5 2 749.5 3 638.5 4 371.0 5 257.5 6 255.5 7 269.0 8 280.5 9 291.5 10 240.5 11 201.0 12 190.5 13 190.0 14 214.5 15 226.5 16 211.0 17 229.0 18 281.0 19 327.5 20 375.5 21 441.5 22 533.0 23 664.5 24 821.5 25 1236.5 26 2306.5 27 3573.0 28 3959.5 29 4880.0 30 7681.0 31 9843.5 32 13176.5 33 18788.0 34 24493.5 35 28137.5 36 32729.0 37 36944.0 38 35634.5 39 33946.0 40 30707.0 41 26572.0 42 22870.0 43 21736.5 44 22071.0 45 20060.5 46 16431.5 47 12332.0 48 10185.0 49 8263.0 50 6345.5 51 4379.0 52 3003.0 53 2329.5 54 1696.0 55 1307.0 56 1079.5 57 881.5 58 727.0 59 604.5 60 483.0 61 425.5 62 381.0 63 265.0 64 166.0 65 136.0 66 117.0 67 98.0 68 93.5 69 89.0 70 80.5 71 69.0 72 56.0 73 43.5 74 35.5 75 35.0 76 33.0 77 23.5 78 16.5 79 18.5 80 14.5 81 9.5 82 9.0 83 9.5 84 8.0 85 4.0 86 3.5 87 2.5 88 2.5 89 3.5 90 2.0 91 1.0 92 3.0 93 3.0 94 3.0 95 3.0 96 3.5 97 4.0 98 3.0 99 6.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013644172387916223 2 0.008062465501950496 3 0.005168247116634933 4 0.023773936736520694 5 0.02108644823587053 6 0.021293178120535928 7 0.007028816078623511 8 6.20189653996192E-4 9 0.009509574694608279 10-11 1.0336494233269867E-4 12-13 0.002894218385315563 14-15 0.014471091926577815 16-17 8.269195386615894E-4 18-19 0.0068220861939581115 20-21 0.0019639339043212747 22-23 4.134597693307947E-4 24-25 0.005891801712963825 26-27 4.134597693307947E-4 28-29 0.004237962635640646 30-31 6.20189653996192E-4 32-33 8.269195386615894E-4 34-35 1.0336494233269867E-4 36-37 0.005271612058967632 38-39 5.168247116634934E-4 40-41 0.010439859175602566 42-43 0.005581706885965728 44-45 8.269195386615894E-4 46-47 0.009406209752275579 48-49 0.0018605689619885762 50-51 0.007338910905621605 52-53 0.0034110430969790557 54-55 0.0020672988466539735 56-57 0.007545640790287003 58-59 0.014367726984245117 60-61 0.0010336494233269867 62-63 0.005581706885965728 64-65 0.0013437442503250827 66-67 9.302844809942881E-4 68-69 1.0336494233269867E-4 70-71 0.0034110430969790557 72-73 2.0672988466539734E-4 74-75 0.004237962635640646 76-77 0.012300428137591142 78-79 0.001240379307992384 80-81 0.002170663788986672 82-83 8.269195386615894E-4 84-85 1.0336494233269867E-4 86-87 0.0013437442503250827 88-89 0.0035144080393117554 90-91 0.004341327577973344 92-93 0.012817252849254635 94-95 0.0 96-97 5.168247116634934E-4 98-99 0.0019639339043212747 100-101 0.0 102-103 1.0336494233269867E-4 104-105 0.004961517231969536 106-107 0.0 108-109 0.0 110-111 2.0672988466539734E-4 112-113 0.0037211379239771524 114-115 0.0017572040196558777 116-117 1.0336494233269867E-4 118-119 0.004341327577973344 120-121 0.0014471091926577815 122-123 1.0336494233269867E-4 124-125 0.07452612342187574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 483723.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.771182436422826 #Duplication Level Percentage of deduplicated Percentage of total 1 75.23569460633699 15.627343383992184 2 8.356376501881828 3.4714364165604845 3 3.512836817906833 2.1889732324237756 4 1.9708623897553073 1.6374856901876702 5 1.356904342414545 1.4092253822533434 6 0.9369339307947604 1.1676735364447637 7 0.749947116009822 1.0904101855016417 8 0.6199562825580656 1.0301780038096062 9 0.5219631927341272 0.9757613431240455 >10 4.907653927086321 22.493812094865 >50 1.0059290649243764 14.763738517089307 >100 0.7969438019331292 29.914209179963404 >500 0.024998237199909953 3.3360320760447424 >1k 0.0029997884639891945 0.8937209577400401 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1966 0.4064309532521712 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1295 0.2677152006416896 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1042 0.21541253982134403 No Hit GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC 870 0.1798549996588957 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 859 0.17758097092757633 No Hit GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT 837 0.17303291346493757 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 806 0.16662428704031026 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 802 0.16579736750164867 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 719 0.1486387870744207 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 702 0.14512437903510894 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 687 0.14202343076512797 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 679 0.1403695916878048 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 676 0.1397494020338086 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 618 0.12775906872321557 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 596 0.12321101126057682 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 596 0.12321101126057682 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 588 0.12155717218325364 No Hit ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA 587 0.12135044229858824 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 573 0.11845622391327268 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 562 0.1161821951819533 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 557 0.11514854575862632 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 551 0.11390816645063394 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 545 0.11266778714264156 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 545 0.11266778714264156 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 532 0.10998029864199138 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 529 0.1093601089879952 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 525 0.10853318944933361 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 521 0.10770626991067202 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 498 0.10295148256336789 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 486 0.1004707239473831 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.10094826998096E-4 0.0 18-19 0.0 0.0 0.0 6.20189653996192E-4 0.0 20-21 0.0 0.0 0.0 0.001653839077323179 0.0 22-23 0.0 0.0 0.0 0.004134597693307947 0.0 24-25 0.0 0.0 0.0 0.007649005732619701 0.0 26-27 0.0 0.0 0.0 0.014987916638241307 0.0 28-29 0.0 0.0 0.0 0.025841235583174665 0.0 30-31 0.0 0.0 0.0 0.06026176137996333 0.0 32-33 0.0 0.0 0.0 0.12083361758692474 0.0 34-35 0.0 0.0 0.0 0.21014092776237642 0.0 36-37 0.0 0.0 0.0 0.33903701085125165 0.0 38-39 0.0 0.0 0.0 0.5357404961103772 0.0 40-41 0.0 0.0 0.0 0.7728596738215879 0.0 42-43 0.0 0.0 0.0 0.9797962883716507 0.0 44-45 0.0 0.0 0.0 1.2142279775822113 0.0 46-47 0.0 0.0 0.0 1.4837003822435566 0.0 48-49 0.0 0.0 0.0 1.772502031121117 0.0 50-51 0.0 0.0 0.0 2.088385294889844 0.0 52-53 0.0 0.0 0.0 2.373775900670425 0.0 54-55 0.0 0.0 0.0 2.6772553713592284 0.0 56-57 0.0 0.0 0.0 3.0274557959824113 0.0 58-59 0.0 0.0 0.0 3.432439640041925 0.0 60-61 0.0 0.0 0.0 3.869776711051573 0.0 62-63 0.0 0.0 0.0 4.292022500480647 0.0 64-65 0.0 0.0 0.0 4.722020660584674 0.0 66-67 0.0 0.0 0.0 5.185612426946827 0.0 68-69 0.0 0.0 0.0 5.693341023685043 0.0 70-71 0.0 0.0 0.0 6.27466545936414 0.0 72-73 0.0 0.0 0.0 6.866739849045838 0.0 74-75 0.0 0.0 0.0 7.491891020274 0.0 76-77 0.0 0.0 0.0 8.108049441519217 0.0 78-79 0.0 0.0 0.0 8.795426308031663 0.0 80-81 0.0 0.0 0.0 9.58968252491612 0.0 82-83 0.0 0.0 0.0 10.343212954521492 0.0 84-85 0.0 0.0 0.0 11.1237216340757 0.0 86-87 0.0 0.0 0.0 11.923559557846122 0.0 88-89 0.0 0.0 0.0 12.769808340723927 0.0 90-91 0.0 0.0 0.0 13.708672111931829 0.0 92-93 0.0 0.0 0.0 14.73022783700589 0.0 94-95 0.0 0.0 0.0 15.741240337962015 0.0 96-97 0.0 0.0 0.0 16.779024358982312 0.0 98-99 0.0 0.0 0.0 17.827248239178207 0.0 100-101 0.0 0.0 0.0 18.985659147900762 0.0 102-103 0.0 0.0 0.0 20.220766843834177 0.0 104-105 0.0 0.0 0.0 21.46135288171123 0.0 106-107 0.0 0.0 0.0 22.69914806614529 0.0 108-109 0.0 0.0 0.0 23.88515741447068 0.0 110-111 0.0 0.0 0.0 25.160474072971514 0.0 112-113 0.0 0.0 0.0 26.486439553215373 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 60 3.943569E-9 69.45734 2 GGTATCA 565 0.0 68.49143 1 GTATGGT 195 0.0 67.16754 1 GTACATT 35 0.0077755125 51.02988 1 GTATCAA 1140 0.0 48.04568 1 CATATAG 215 0.0 47.074074 3 ACATATA 230 0.0 44.00403 2 CTCCTCG 155 1.8189894E-12 42.228703 9 GTACATG 2175 0.0 41.879696 1 AGGTTAG 90 5.128608E-6 39.689907 1 TGGGTAT 120 2.0667358E-8 39.677593 6 GTGGTAC 330 0.0 39.677593 6 CATGGGT 335 0.0 39.097523 4 GGTACTC 335 0.0 39.07731 8 TGGTACT 340 0.0 38.49866 7 TCAACGC 1240 0.0 38.409588 4 ATGGGTA 220 0.0 37.885822 5 CAACGCA 1280 0.0 36.74417 5 GGTATGG 65 0.0019371378 36.6179 8 ACATGGG 2165 0.0 36.57338 3 >>END_MODULE