FastQCFastQC Report
Fri 27 May 2016
SRR940139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483723
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36100.7462948836420844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.6261848206514885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.3657051659730879No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT17550.3628109475877723No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT11980.247662401829146No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT9210.19039822377683097No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT9040.1868838157375192No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC8710.1800617295435611No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT8650.17882135023556872RNA PCR Primer, Index 26 (100% over 22bp)
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT8060.16662428704031026No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7470.1544272238450518No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT7360.15215319511373246No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC7340.15173973534440163No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA7050.14574456868910513No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA6530.13499461468650448No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG6510.13458115491717368No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6470.13375423537851208No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA6160.12734560895388475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.1263119595305578No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6100.12610522964589238No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT6070.1254850399918962No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA5960.12321101126057682No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG5820.12031679287526126No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT5780.11948987333659966RNA PCR Primer, Index 26 (100% over 24bp)
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5420.11204759748864536No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.11060048829598758No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5310.109773568757326No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC5300.1095668388726606No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5290.1093601089879952No Hit
TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT5270.1089466492186644No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA5120.10584570094868344No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT5120.10584570094868344No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5100.10543224117935265No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT5090.10522551129468725No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG5050.10439859175602566No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT4930.1019178331400409No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA4910.1015043733707101No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA4900.1012976434860447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.092.080341
GTATCAA30100.062.6908951
CGGGGTA150.004235766459.5020744-45
GTATGGT1650.054.115161
ATTGTCA350.007779735651.022871
GTAAGAG350.007779735651.022873
TCAACGC38300.049.11344
CAACGCA38750.048.676535
AACGCAG39150.048.1791956
ATCAACG40600.046.3311163
TATCAAC40950.045.9351232
AAGACAT1501.8189894E-1243.6348535
GGTACTC2500.042.859213
GGTTTGC705.4494783E-542.4970868
ACGCAGA44800.041.833077
CGCAGAG44950.041.5611088
GTGGTAC2600.041.210781
ACTCCCG2800.038.251336
GCAGAGT48300.037.8162469
TGGTACT2850.037.5958022
Produced by FastQC (version 0.11.5)