##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940139_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 483723 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63602723046041 34.0 31.0 34.0 31.0 34.0 2 32.721313644379116 34.0 31.0 34.0 31.0 34.0 3 32.802506806581455 34.0 31.0 34.0 31.0 34.0 4 36.25074267711066 37.0 37.0 37.0 35.0 37.0 5 36.22854195479644 37.0 37.0 37.0 35.0 37.0 6 36.300146984948 37.0 37.0 37.0 35.0 37.0 7 36.26538328754266 37.0 37.0 37.0 35.0 37.0 8 36.25230348773989 37.0 37.0 37.0 35.0 37.0 9 38.0674373556767 39.0 39.0 39.0 35.0 39.0 10-11 38.03520299841025 39.0 39.0 39.0 35.0 39.0 12-13 38.06017700212725 39.0 39.0 39.0 35.0 39.0 14-15 39.476801805992274 41.0 39.0 41.0 36.0 41.0 16-17 39.453542626668565 41.0 39.0 41.0 36.0 41.0 18-19 39.573156537935965 41.0 39.5 41.0 36.5 41.0 20-21 39.58677590273773 41.0 40.0 41.0 37.0 41.0 22-23 39.5344567035266 41.0 39.5 41.0 36.0 41.0 24-25 39.50845318498397 41.0 39.0 41.0 36.0 41.0 26-27 39.383581099100105 41.0 39.0 41.0 36.0 41.0 28-29 39.2739015924403 41.0 39.0 41.0 35.5 41.0 30-31 39.08520062101657 41.0 39.0 41.0 35.0 41.0 32-33 38.840479985446215 41.0 38.0 41.0 35.0 41.0 34-35 38.69298131368573 40.5 38.0 41.0 35.0 41.0 36-37 38.538075510157675 40.0 38.0 41.0 35.0 41.0 38-39 38.357106236420435 40.0 38.0 41.0 35.0 41.0 40-41 38.27476779065705 40.0 38.0 41.0 35.0 41.0 42-43 38.06873148475471 40.0 38.0 41.0 34.0 41.0 44-45 38.032711903300026 40.5 38.0 41.0 34.0 41.0 46-47 37.67007874341307 41.0 38.0 41.0 33.0 41.0 48-49 37.25963206215127 41.0 38.0 41.0 32.5 41.0 50-51 37.058367081987 41.0 38.0 41.0 31.0 41.0 52-53 37.008995850931214 40.5 38.0 41.0 31.0 41.0 54-55 36.86754299464776 40.0 38.0 41.0 31.0 41.0 56-57 36.74834564409797 40.0 38.0 41.0 31.0 41.0 58-59 36.57929434821168 40.0 37.0 41.0 31.0 41.0 60-61 36.40008951404006 40.0 37.0 41.0 30.5 41.0 62-63 36.155555969015325 40.0 36.5 41.0 29.5 41.0 64-65 35.88780459064382 39.0 36.0 41.0 29.0 41.0 66-67 35.55598865466393 39.0 36.0 41.0 28.0 41.0 68-69 35.17034232401602 38.5 35.0 41.0 28.0 41.0 70-71 34.83494375913487 37.5 35.0 40.0 28.0 41.0 72-73 34.55299623958339 37.0 35.0 39.5 28.0 41.0 74-75 34.17455237811723 37.0 35.0 39.0 28.0 41.0 76-77 33.80372961384924 36.0 35.0 39.0 27.5 41.0 78-79 33.41854739179241 36.0 35.0 37.5 26.0 39.5 80-81 33.05709879414458 35.5 35.0 37.0 26.0 39.0 82-83 32.7184194259938 35.0 34.5 37.0 26.0 39.0 84-85 32.41290573323989 35.0 34.0 36.0 26.0 38.0 86-87 32.190119138432536 35.0 34.0 36.0 26.0 37.0 88-89 31.97001589752813 35.0 34.0 36.0 25.0 37.0 90-91 31.816755663882013 35.0 34.0 35.0 25.0 36.0 92-93 31.683098591549296 35.0 34.0 35.0 25.0 36.0 94-95 31.546852227411144 35.0 34.0 35.0 24.0 36.0 96-97 31.451506337304615 35.0 34.0 35.0 23.5 36.0 98-99 31.380227940370833 35.0 34.0 35.0 23.0 36.0 100-101 31.36698275666032 35.0 34.0 35.0 23.0 36.0 102-103 30.634436443997906 34.5 32.5 35.0 19.5 35.0 104-105 31.20797749951935 35.0 34.0 35.0 20.0 35.0 106-107 31.231652205911235 35.0 34.0 35.0 20.0 35.0 108-109 31.214467784248423 35.0 34.0 35.0 19.0 35.0 110-111 31.141932469615874 35.0 34.0 35.0 18.5 35.0 112-113 31.06145045821679 35.0 34.0 35.0 16.0 35.0 114-115 30.966159351529697 35.0 34.0 35.0 6.0 35.0 116-117 30.838932612259498 35.0 34.0 35.0 2.0 35.0 118-119 30.728492339624125 35.0 34.0 35.0 2.0 35.0 120-121 30.566587695850725 35.0 34.0 35.0 2.0 35.0 122-123 30.373301455585118 35.0 34.0 35.0 2.0 35.0 124-125 28.71191260287396 33.5 29.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21230770528568144 1101 2 0.16434675874543814 1101 3 0.19669364065225636 1101 4 0.039703590652415244 1101 5 0.09223664707800339 1101 6 0.0763321457409134 1101 7 0.09242921046091368 1101 8 0.16651236920381507 1101 9 0.23969809565534916 1101 10-11 0.14484763530841605 1101 12-13 0.12196844204175505 1101 14-15 0.05419243914785454 1101 16-17 0.12279225087528545 1101 18-19 0.1691523574696916 1101 20-21 0.16257955168119764 1101 22-23 0.3045211520757647 1101 24-25 0.17194765807762025 1101 26-27 0.24003266967396542 1101 28-29 0.2954187081984614 1101 30-31 0.42281715331873215 1101 32-33 0.3315921244008351 1101 34-35 0.44691006237511743 1101 36-37 0.45260344975929456 1101 38-39 0.5085367883955669 1101 40-41 0.5193971876310712 1101 42-43 0.5278254861902454 1101 44-45 0.5217095567644847 1101 46-47 0.5790056414071785 1101 48-49 0.4374753643200293 1101 50-51 0.7418508131657546 1101 52-53 0.6450368868007956 1101 54-55 0.5906182054793945 1101 56-57 0.476253606153513 1101 58-59 0.453879146618263 1101 60-61 0.4338256648815104 1101 62-63 0.5040717410450242 1101 64-65 0.38292978686362034 1101 66-67 0.5443516947229128 1101 68-69 0.48308609490261034 1101 70-71 0.38530627557792485 1101 72-73 0.4273614313233054 1101 74-75 0.48778854464332966 1101 76-77 0.5215813953205455 1101 78-79 0.5520494604260762 1101 80-81 0.5329812402697769 1101 82-83 0.46369075437507234 1101 84-85 0.45070107967350026 1101 86-87 0.40737468691550305 1101 88-89 0.4392851208942652 1101 90-91 0.4341078510470986 1101 92-93 0.40297442693699637 1101 94-95 0.43545876662204464 1101 96-97 0.44913689956143443 1101 98-99 0.47983997097993836 1101 100-101 0.4378412830060512 1101 102-103 0.5031238910741855 1101 104-105 0.42792192958531317 1101 106-107 0.41698411662233426 1101 108-109 0.3119883809945989 1101 110-111 0.19989357951472897 1101 112-113 0.3933738647637597 1101 114-115 0.5671863218836961 1101 116-117 0.4992847285537714 1101 118-119 0.31894647510602425 1101 120-121 0.14584011174644118 1101 122-123 0.12331056529231788 1101 124-125 0.16495332533479257 1102 1 0.12920991623653322 1102 2 0.14188197866746322 1102 3 0.20421243764473473 1102 4 0.095928104388463 1102 5 0.03736171829989132 1102 6 0.10790438987279316 1102 7 0.10570608764644618 1102 8 0.15495822381060975 1102 9 0.14860781423113423 1102 10-11 0.19133569796134253 1102 12-13 0.23608098938336042 1102 14-15 0.2260142671204619 1102 16-17 0.2860294672445889 1102 18-19 0.3079864411931226 1102 20-21 0.22482915786605417 1102 22-23 0.2363447675781174 1102 24-25 0.32592770477683075 1102 26-27 0.3263357229982162 1102 28-29 0.30299001387721347 1102 30-31 0.3535905950955325 1102 32-33 0.33943937998407137 1102 34-35 0.2485188391461719 1102 36-37 0.41179638007915287 1102 38-39 0.49485324819290355 1102 40-41 0.354506506247084 1102 42-43 0.32530842086720213 1102 44-45 0.4267839068298471 1102 46-47 0.3585395430260121 1102 48-49 0.5059288159282502 1102 50-51 0.2189492055061919 1102 52-53 0.19531227810223584 1102 54-55 0.44181864342418464 1102 56-57 0.25541212652576917 1102 58-59 0.12462733953741179 1102 60-61 0.1690511729309776 1102 62-63 0.1469713377336248 1102 64-65 0.11198049684808353 1102 66-67 0.14891548160467494 1102 68-69 0.26121678567113094 1102 70-71 0.30732193880731273 1102 72-73 0.31341646489534014 1102 74-75 0.44449904302896925 1102 76-77 0.4967459039174429 1102 78-79 0.32747986407264307 1102 80-81 0.3490466638584664 1102 82-83 0.24250964409566222 1102 84-85 0.39860911126809384 1102 86-87 0.21007609124356463 1102 88-89 0.29290319281998833 1102 90-91 0.3482192811826117 1102 92-93 0.3226562466422713 1102 94-95 0.29754302630542995 1102 96-97 0.37785356492469546 1102 98-99 0.26000732845237096 1102 100-101 0.24157722017437777 1102 102-103 0.258582001431801 1102 104-105 0.23611887067503545 1102 106-107 0.2268903830312965 1102 108-109 0.23772210609487132 1102 110-111 0.31447758782674384 1102 112-113 0.426672192306242 1102 114-115 0.2548065611892376 1102 116-117 0.2987238910960883 1102 118-119 0.48330313197851993 1102 120-121 0.4776480863635193 1102 122-123 0.5930029984554608 1102 124-125 0.5137204884133624 1103 1 0.10953744739319404 1103 2 0.08234043073370145 1103 3 -0.04379342175261769 1103 4 -0.11436246221068558 1103 5 -0.16344623766308075 1103 6 -0.07893256552941352 1103 7 -0.061820033592724144 1103 8 0.02592330775334517 1103 9 0.1511896111892881 1103 10-11 -0.11568642355248926 1103 12-13 0.06834544046540003 1103 14-15 0.12576478496759336 1103 16-17 0.15491629618909286 1103 18-19 0.03924266394177067 1103 20-21 0.14247601473424965 1103 22-23 0.2631595213575437 1103 24-25 0.26067438883858074 1103 26-27 0.1407679222177265 1103 28-29 0.33129364967904706 1103 30-31 0.3093143794422417 1103 32-33 0.30333771028470835 1103 34-35 0.31622463331194695 1103 36-37 0.2124612843489544 1103 38-39 0.43974058067937705 1103 40-41 0.4527235099435316 1103 42-43 0.446626556388658 1103 44-45 0.34476722128035675 1103 46-47 0.4089601150039073 1103 48-49 0.5768103576821773 1103 50-51 0.4801169650877952 1103 52-53 0.5200235997854392 1103 54-55 0.4613892594619955 1103 56-57 0.4759200172571596 1103 58-59 0.49131460564789364 1103 60-61 0.5538407725006849 1103 62-63 0.4257710768073366 1103 64-65 0.2560461404090475 1103 66-67 0.27200745812131544 1103 68-69 0.23485750848272602 1103 70-71 0.3218067810499079 1103 72-73 0.17006035773245998 1103 74-75 0.28447798581973416 1103 76-77 0.26963131378915506 1103 78-79 0.18267721818851612 1103 80-81 0.18650789684962632 1103 82-83 0.1483621749662163 1103 84-85 0.2295712197356039 1103 86-87 0.26139731668158817 1103 88-89 0.27081028932838436 1103 90-91 0.3424308374705909 1103 92-93 0.22135619285483799 1103 94-95 0.3023873288425918 1103 96-97 0.24319042305174676 1103 98-99 0.48915897469125724 1103 100-101 0.34190337803478243 1103 102-103 0.3703374173689049 1103 104-105 0.28698118628140534 1103 106-107 0.2619998461869031 1103 108-109 0.16621342571812647 1103 110-111 0.036874905321393925 1103 112-113 0.12973120971277297 1103 114-115 -0.03930543112030449 1103 116-117 0.08795152639437731 1103 118-119 0.021525399977281978 1103 120-121 0.022255037531390798 1103 122-123 0.06500148782958348 1103 124-125 0.06047893704636209 1104 1 0.15147080864222318 1104 2 0.04155693023852791 1104 3 0.1108130001864609 1104 4 0.05545136669269368 1104 5 -0.09901415588338836 1104 6 0.03085587338506457 1104 7 0.050288066611329896 1104 8 0.14933061767737854 1104 9 0.18403043191070623 1104 10-11 0.24386439962817263 1104 12-13 0.12426323539138906 1104 14-15 0.17143020872119052 1104 16-17 0.1810639967999208 1104 18-19 0.1936164550924957 1104 20-21 0.1868619691519129 1104 22-23 0.21695240019678863 1104 24-25 0.15019470415300162 1104 26-27 0.1860877230857696 1104 28-29 0.2984229192848389 1104 30-31 0.24969110838749486 1104 32-33 0.1654607653251503 1104 34-35 0.1661644816366703 1104 36-37 0.23825006888631606 1104 38-39 0.3010029018029101 1104 40-41 0.1480602596261562 1104 42-43 0.2156773563062444 1104 44-45 0.12192021922655982 1104 46-47 0.11422022945825461 1104 48-49 -0.10715739304396976 1104 50-51 -0.09581040093348747 1104 52-53 0.029850588944839274 1104 54-55 0.010255018203928046 1104 56-57 -0.13636754098361337 1104 58-59 -0.16269029953843983 1104 60-61 -0.30524811240024974 1104 62-63 -0.2532781943936371 1104 64-65 -0.15081513852856432 1104 66-67 -0.26341556435082225 1104 68-69 -0.4705887106022999 1104 70-71 -0.5351751335679538 1104 72-73 -0.456949406114056 1104 74-75 -0.47454310382288156 1104 76-77 -0.32681408776481646 1104 78-79 -0.3801297713696812 1104 80-81 -0.3472169208675382 1104 82-83 -0.3426782163852309 1104 84-85 -0.36808053862082346 1104 86-87 -0.27369337609290056 1104 88-89 -0.12988959701485925 1104 90-91 -0.2147963364641221 1104 92-93 -0.23971802965983713 1104 94-95 -0.18772253935544825 1104 96-97 -0.03473357979299507 1104 98-99 -0.05352212837747672 1104 100-101 -0.17474633981730392 1104 102-103 -0.056613191791431206 1104 104-105 -0.1188963967511718 1104 106-107 -0.08505870365922874 1104 108-109 -0.08859040006134933 1104 110-111 -0.02968740982021245 1104 112-113 -0.12318498338407835 1104 114-115 0.00173063994508027 1104 116-117 0.0828402956445764 1104 118-119 0.016879430804184636 1104 120-121 0.1459980004500423 1104 122-123 0.027634336626217504 1104 124-125 0.3358007983585338 1105 1 -0.044787120150054704 1105 2 -0.06133409070304907 1105 3 -0.045642125771102826 1105 4 -0.04874013555446055 1105 5 -0.28843818291299783 1105 6 0.06247329388464351 1105 7 -0.09478784792239736 1105 8 -0.01885221469441234 1105 9 -0.1422543777451537 1105 10-11 0.05999823020435002 1105 12-13 0.005797250839925994 1105 14-15 0.029532186005262417 1105 16-17 0.04493951608932889 1105 18-19 0.09143411146368408 1105 20-21 0.10003359230982056 1105 22-23 -0.02612710553532338 1105 24-25 0.05575043678934577 1105 26-27 0.15194902632953244 1105 28-29 0.1256342156224548 1105 30-31 -0.11725074182341189 1105 32-33 -0.02728033902147331 1105 34-35 -0.2693012585433081 1105 36-37 -0.08651326264079273 1105 38-39 0.13722194365418972 1105 40-41 0.011336537529665236 1105 42-43 -0.19004199897802465 1105 44-45 -0.12947535446207326 1105 46-47 -0.2733083248187853 1105 48-49 -0.5622068517079981 1105 50-51 -0.47625961947596807 1105 52-53 -0.5190502706053053 1105 54-55 -0.5434291475861883 1105 56-57 -0.6974115384761532 1105 58-59 -0.39731986423845456 1105 60-61 -0.29338228297154956 1105 62-63 -0.3813915402642891 1105 64-65 -0.19696522512106895 1105 66-67 -0.34003133799907914 1105 68-69 -0.3737538526033788 1105 70-71 -0.31682602233642143 1105 72-73 -0.11948513216299261 1105 74-75 -0.15939207161896718 1105 76-77 -0.16196108252676567 1105 78-79 -0.23188021495705868 1105 80-81 -0.18457388386119788 1105 82-83 -0.16714405218884565 1105 84-85 -0.361398572174096 1105 86-87 -0.32502626318265015 1105 88-89 -0.29901272836984916 1105 90-91 -0.32549237020863586 1105 92-93 -0.2793359601520855 1105 94-95 -0.2699121078804012 1105 96-97 -0.3021694854018513 1105 98-99 -0.26500227585035674 1105 100-101 -0.2500807568850618 1105 102-103 -0.2039118243735878 1105 104-105 -0.27708018535920687 1105 106-107 -0.19342683336736144 1105 108-109 -0.2021275179652804 1105 110-111 -0.19944590704181664 1105 112-113 -0.15767005016124358 1105 114-115 -0.17792490250649706 1105 116-117 -0.14515405193667874 1105 118-119 -0.20725172231049172 1105 120-121 -0.14609923815215353 1105 122-123 -0.22379629905839238 1105 124-125 -0.18598863841831204 1106 1 0.03792736202491653 1106 2 0.03535479113340756 1106 3 0.08220088702906736 1106 4 0.004809002285377062 1106 5 0.03958466697592655 1106 6 -0.03434253177916702 1106 7 0.09820908426844 1106 8 -0.12341161382695987 1106 9 0.0438241158547612 1106 10-11 -0.09791460170085742 1106 12-13 -0.01635444703376976 1106 14-15 -0.1400912139735837 1106 16-17 0.05224936481459963 1106 18-19 -0.050235199866051516 1106 20-21 0.10529118249283442 1106 22-23 -0.11889659670214314 1106 24-25 -0.17293357886706673 1106 26-27 0.02351889832785048 1106 28-29 0.03652493814440305 1106 30-31 0.019303791129090087 1106 32-33 0.054651322884801345 1106 34-35 0.17042687009310242 1106 36-37 -0.007845114962805155 1106 38-39 0.12496657498815722 1106 40-41 -0.20739825565768655 1106 42-43 0.08542610638535564 1106 44-45 0.006788265552735595 1106 46-47 -0.2485542784842778 1106 48-49 -0.03894968467107418 1106 50-51 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0.35104101609728744 2207 94-95 0.40850872507116875 2207 96-97 0.40446297548037435 2207 98-99 0.3181076000518708 2207 100-101 0.2862873217882438 2207 102-103 0.34644601292497157 2207 104-105 0.17864673846165502 2207 106-107 0.16666030994062098 2207 108-109 0.20861167310863848 2207 110-111 0.25660067797890207 2207 112-113 0.18550966255089207 2207 114-115 0.3594164121765431 2207 116-117 0.29154590428806415 2207 118-119 0.320153626415447 2207 120-121 0.5724960850212462 2207 122-123 0.5526688996100226 2207 124-125 0.3021696447770772 2208 1 0.12515901357882342 2208 2 0.014351923583227233 2208 3 0.005517170064024413 2208 4 0.012431260674901523 2208 5 0.0460947809029193 2208 6 0.10162197259965922 2208 7 0.023548152702133507 2208 8 0.12628225581575947 2208 9 0.1625135679201719 2208 10-11 0.07364240054668159 2208 12-13 0.03041374640961436 2208 14-15 0.08099496753460045 2208 16-17 0.148618426273174 2208 18-19 0.14294080571404777 2208 20-21 0.12477889852787172 2208 22-23 0.2210146642449189 2208 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-0.19117361675566258 2215 114-115 -0.22506290770483872 2215 116-117 -0.2670933824632371 2215 118-119 -0.23469664562248127 2215 120-121 -0.2563746838726537 2215 122-123 -0.277736991451043 2215 124-125 -0.3378358769026555 2216 1 -0.4133069856312659 2216 2 -0.24581822142244647 2216 3 -0.32733918467013723 2216 4 -0.1511044829415269 2216 5 -0.2787292124266898 2216 6 -0.31335097597441575 2216 7 -0.3620318386464234 2216 8 -0.39341857986328677 2216 9 -0.4373964696320982 2216 10-11 -0.44815076732262327 2216 12-13 -0.36414995139178785 2216 14-15 -0.6180046312391525 2216 16-17 -0.51312433754876 2216 18-19 -0.5800328450059666 2216 20-21 -0.6031411195273151 2216 22-23 -0.650825679054293 2216 24-25 -0.535469046186158 2216 26-27 -0.5548787434140081 2216 28-29 -0.6506957832651139 2216 30-31 -0.5955856867579854 2216 32-33 -0.7468540205747729 2216 34-35 -0.8855299404816819 2216 36-37 -0.7858774645558881 2216 38-39 -0.7127528126802574 2216 40-41 -0.9088832107182512 2216 42-43 -0.7847048890782276 2216 44-45 -0.7273232250091368 2216 46-47 -0.7234867419125948 2216 48-49 -0.5733508604020869 2216 50-51 -0.4053762623251629 2216 52-53 -0.4141607009456152 2216 54-55 -0.42443050817698236 2216 56-57 -0.6057034408216708 2216 58-59 -0.7460854917788637 2216 60-61 -0.7807835545677193 2216 62-63 -0.8436834939528595 2216 64-65 -0.7406590151793395 2216 66-67 -0.5471320816175691 2216 68-69 -0.5151911156766076 2216 70-71 -0.5454198218075916 2216 72-73 -0.3356496352906433 2216 74-75 -0.3067962526545571 2216 76-77 -0.4149941731215776 2216 78-79 -0.3364351990834962 2216 80-81 -0.49677128076863397 2216 82-83 -0.4135983868257185 2216 84-85 -0.4060678801553692 2216 86-87 -0.47705645289521215 2216 88-89 -0.5062135909330614 2216 90-91 -0.6525184952631911 2216 92-93 -0.5797210330164297 2216 94-95 -0.4287683559015534 2216 96-97 -0.48189839410535384 2216 98-99 -0.3909151124682211 2216 100-101 -0.6376557391289239 2216 102-103 -0.7176169954742946 2216 104-105 -0.6680883061180616 2216 106-107 -0.5706741613895439 2216 108-109 -0.6634405308609423 2216 110-111 -0.6596764373299315 2216 112-113 -0.6769382405754953 2216 114-115 -0.7429166448328495 2216 116-117 -0.7728236765334202 2216 118-119 -0.6754104750166938 2216 120-121 -0.6703109084270942 2216 122-123 -0.7143483224023655 2216 124-125 -1.0688434830628069 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 8.0 5 27.0 6 70.0 7 203.0 8 417.0 9 703.0 10 1401.0 11 2025.0 12 2395.0 13 3728.0 14 7154.0 15 7721.0 16 2484.0 17 1638.0 18 1342.0 19 1505.0 20 1653.0 21 2134.0 22 2253.0 23 1228.0 24 1437.0 25 1722.0 26 2268.0 27 2666.0 28 3402.0 29 4328.0 30 5414.0 31 6710.0 32 8771.0 33 11606.0 34 15927.0 35 25027.0 36 45135.0 37 181333.0 38 127683.0 39 197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.17215196155468 24.53101349059959 15.793554950826724 21.503279597019 2 14.055115668479049 23.97717640735151 42.158524735895476 19.809183188273966 3 14.619054903890284 31.877664258927723 33.724194675410054 19.779086161771943 4 9.712914205315485 21.35194132069811 43.86939924064514 25.065745233341264 5 9.281969436358825 40.62748081503043 38.80511544059274 11.285434308017994 6 23.109784087572528 45.440654064538 19.28262155154232 12.166940296347146 7 22.26728933707928 36.16470583371061 26.851731259419132 14.716273569790975 8 22.919108663429277 36.41319515507842 23.583331782859197 17.0843643986331 9 25.785005054545678 16.018051653528982 24.55930356836454 33.637639723560795 10-11 20.25146623170699 29.208968769316325 32.20882612569591 18.330738873280783 12-13 22.290443084161804 28.526966879805176 31.274613776892974 17.907976259140046 14-15 20.813668153054536 28.88636678429597 29.696851297126663 20.603113765522828 16-17 17.298019734434792 32.32159727778088 30.990773645247383 19.389609342536946 18-19 17.173258249039222 29.734682866020428 35.13891214600091 17.953146738939434 20-21 17.60749437177889 29.33910523171319 35.53231911651917 17.521081279988753 22-23 18.137032971349303 28.991075470878997 35.027794832993266 17.84409672477844 24-25 17.955916463724733 30.052737338697337 34.99962788236121 16.991718315216715 26-27 17.33057969126959 29.68258693508475 35.864327311291795 17.122506062353867 28-29 17.208562418017916 27.77214433601361 37.053368849857925 17.965924396110548 30-31 18.079647371620197 28.240455505890232 35.597665408264106 18.082231714225465 32-33 17.62785726541843 29.287112665719846 35.74907540059083 17.33595466827089 34-35 18.209801890751525 28.714367520254356 35.868978733696764 17.20685185529735 36-37 18.470047816820028 28.164789207001554 34.52734117394118 18.83782180223724 38-39 17.26246219427234 29.18054341017483 35.14573423219487 18.411260163357955 40-41 17.510124596101488 28.333881167527696 36.18031394000285 17.975680296367962 42-43 18.157395864988846 28.151545409252815 35.01952563760665 18.67153308815169 44-45 17.354720066653503 28.83074165461535 34.8625323163023 18.952005962428842 46-47 18.686370115700566 28.831282670964047 33.375783405398536 19.106563807936855 48-49 18.431312962170498 29.666668733965512 32.576908685342644 19.325109618521346 50-51 18.040459733045562 29.22192900578956 32.753757115494544 19.98385414567034 52-53 18.346967169227018 29.76796637745156 32.818265825689494 19.06680062763193 54-55 18.613004760108122 29.73232790477716 32.455357835055274 19.199309500059435 56-57 18.718105356077828 29.623967510230575 32.030931021259136 19.62699611243246 58-59 17.842966595411262 28.967285913344053 33.49265876379454 19.697088727450147 60-61 18.83740834075841 30.57837058965809 31.41139210412614 19.172828965457363 62-63 18.300731967256326 30.260194150670927 32.60722374464153 18.831850137431218 64-65 18.316535905148797 29.44346244095058 32.34967593884702 19.890325715053596 66-67 18.029843526150298 29.919085923141964 31.93656286759158 20.114507683116166 68-69 18.049978034472957 29.635062149520635 32.28343282424994 20.03152699175647 70-71 18.846870277299406 29.336614974953534 32.16894321078517 19.647571536961884 72-73 18.7225491543925 29.10603662765877 31.692837894131863 20.47857632381687 74-75 17.908389718909376 29.829571883081847 31.519588690221468 20.74244970778731 76-77 17.84826796331671 29.342702622243173 32.70681898996916 20.10221042447096 78-79 17.85707640687763 28.572728043281863 32.07201377676432 21.498181773076183 80-81 18.02350941968614 29.982365867928003 31.125380126147096 20.868744586238762 82-83 18.488668372225238 28.791896012610795 31.524821045559086 21.194614569604877 84-85 17.884719147115188 28.830446350493983 31.110470248468648 22.17436425392218 86-87 17.873504761195942 29.481788373833478 31.54876349487908 21.0959433700915 88-89 18.26231494249547 28.41249036078073 31.702539378665744 21.622655318058058 90-91 18.383251359850153 28.351867816983084 31.790722627782497 21.474158195384263 92-93 18.439863672590935 28.58244510671641 31.502299517147918 21.47539170354474 94-95 18.00245194315461 27.506729405538167 31.965606638853167 22.525212012454052 96-97 18.56196435198154 27.743039870664038 31.39601593131301 22.298979846041412 98-99 17.98539137913568 27.866884989673075 31.125190464081108 23.022533167110133 100-101 17.81549609522382 28.050402881936726 31.60082902197115 22.5332720008683 102-103 18.545267732178925 27.220695828707637 31.18235888784832 23.051677551265115 104-105 17.80320375594231 27.854401417367924 31.24380444209227 23.098590384597493 106-107 18.288190324194474 27.326646296839918 31.020090051723926 23.36507332724168 108-109 18.088132831898843 27.69646811211568 30.137136406583704 24.078262649401772 110-111 17.79690538038765 28.065340735832695 30.27492684157156 23.8628270422081 112-113 17.38855833915643 27.52012586157707 31.39841143173746 23.692904367529035 114-115 17.460302693897127 28.207672572939472 30.351564841861972 23.980459891301425 116-117 17.254520928838332 28.362645938528807 30.106517683175788 24.276315449457076 118-119 17.36202920512325 27.26504057645336 30.592196048718616 24.780734169704775 120-121 17.055527991399984 27.938476804763084 30.323741007194243 24.682254196642685 122-123 17.268710991848472 28.082251252292167 30.445739519095 24.20329823676436 124-125 16.998305184645947 28.169402947262185 29.907234969242047 24.92505689884982 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 627.0 1 421.0 2 267.5 3 287.0 4 173.5 5 128.5 6 290.5 7 642.0 8 1080.0 9 1205.5 10 830.0 11 474.5 12 405.5 13 429.0 14 460.0 15 492.0 16 564.0 17 641.0 18 727.0 19 820.0 20 890.0 21 980.0 22 1092.5 23 1267.0 24 1460.5 25 1871.0 26 2836.0 27 3939.5 28 4303.5 29 5270.5 30 7912.0 31 9805.0 32 13103.5 33 18591.0 34 24087.0 35 27735.0 36 32036.5 37 35667.5 38 34637.5 39 33405.0 40 30117.0 41 26002.5 42 22506.0 43 21542.0 44 21888.0 45 19957.5 46 16539.0 47 12618.0 48 10447.0 49 8444.0 50 6405.5 51 4383.0 52 2958.0 53 2202.5 54 1519.5 55 1130.0 56 884.0 57 676.0 58 501.0 59 390.5 60 323.0 61 264.5 62 204.0 63 115.0 64 51.5 65 26.5 66 15.5 67 9.5 68 8.5 69 6.0 70 7.5 71 8.0 72 8.0 73 7.5 74 3.0 75 2.5 76 2.0 77 2.5 78 2.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026254695352505464 2 0.0026874885006501655 3 0.0010336494233269867 4 0.08641309179013609 5 0.002274028731319371 6 0.020879718351205133 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010336494233269867 26-27 0.0 28-29 9.302844809942881E-4 30-31 0.008475925271281291 32-33 0.0 34-35 0.0 36-37 4.134597693307947E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.005685071828298427 46-47 0.0036177729816444537 48-49 0.0 50-51 0.00155047413499048 52-53 0.0 54-55 0.0032043132123136587 56-57 3.10094826998096E-4 58-59 0.0035144080393117554 60-61 4.134597693307947E-4 62-63 0.00547834194363303 64-65 0.0037211379239771524 66-67 0.0 68-69 0.002170663788986672 70-71 0.004548057462638742 72-73 0.0036177729816444537 74-75 0.0 76-77 0.00310094826998096 78-79 0.0020672988466539735 80-81 4.134597693307947E-4 82-83 0.002170663788986672 84-85 0.0 86-87 0.004341327577973344 88-89 0.004548057462638742 90-91 0.00620189653996192 92-93 0.007132181020956209 94-95 0.005168247116634933 96-97 0.004444692520306042 98-99 0.007442275847954305 100-101 0.004237962635640646 102-103 0.002480758615984768 104-105 0.0019639339043212747 106-107 2.0672988466539734E-4 108-109 0.009096114925277484 110-111 0.0025841235583174667 112-113 0.0035144080393117554 114-115 0.0 116-117 1.0336494233269867E-4 118-119 0.0013437442503250827 120-121 6.20189653996192E-4 122-123 0.0020672988466539735 124-125 0.039175313144092797 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 483723.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.326445093576282 #Duplication Level Percentage of deduplicated Percentage of total 1 70.45888416933136 12.20801987914571 2 9.659714599341383 3.347370292502114 3 4.1080036271655604 2.1353129787088894 4 2.370782226888751 1.643089123320578 5 1.710972175822078 1.482253273050899 6 1.169283634801699 1.2155717218325364 7 0.946165226936477 1.1475575897776207 8 0.718274232806758 0.9956111245485536 9 0.6299813869135685 0.9823804119299682 >10 5.989595761943397 22.907945249657345 >50 1.2313272562401565 15.063786505913509 >100 0.9652555719944639 30.556950982277048 >500 0.03579439698372548 3.965492647651652 >1k 0.005965732830620914 2.3486582196835792 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3610 0.7462948836420844 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3029 0.6261848206514885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1769 0.3657051659730879 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 1755 0.3628109475877723 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1198 0.247662401829146 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 921 0.19039822377683097 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 904 0.1868838157375192 No Hit GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC 871 0.1800617295435611 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 865 0.17882135023556872 RNA PCR Primer, Index 26 (100% over 22bp) GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT 806 0.16662428704031026 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 747 0.1544272238450518 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 736 0.15215319511373246 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 734 0.15173973534440163 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 705 0.14574456868910513 No Hit ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA 653 0.13499461468650448 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 651 0.13458115491717368 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 647 0.13375423537851208 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 616 0.12734560895388475 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 611 0.1263119595305578 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 610 0.12610522964589238 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 607 0.1254850399918962 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 596 0.12321101126057682 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 582 0.12031679287526126 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 578 0.11948987333659966 RNA PCR Primer, Index 26 (100% over 24bp) GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 542 0.11204759748864536 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 535 0.11060048829598758 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 531 0.109773568757326 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 530 0.1095668388726606 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 529 0.1093601089879952 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 527 0.1089466492186644 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 512 0.10584570094868344 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 512 0.10584570094868344 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 510 0.10543224117935265 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 509 0.10522551129468725 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 505 0.10439859175602566 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 493 0.1019178331400409 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 491 0.1015043733707101 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 490 0.1012976434860447 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0336494233269867E-4 0.0 12-13 0.0 0.0 0.0 2.0672988466539734E-4 0.0 14-15 0.0 0.0 0.0 2.0672988466539734E-4 0.0 16-17 0.0 0.0 0.0 5.168247116634934E-4 0.0 18-19 0.0 0.0 0.0 6.20189653996192E-4 0.0 20-21 0.0 0.0 0.0 0.0015504741349904802 0.0 22-23 0.0 0.0 0.0 0.0039278678086425494 0.0 24-25 0.0 0.0 0.0 0.007649005732619701 0.0 26-27 0.0 0.0 0.0 0.015091281580574007 0.0 28-29 0.0 0.0 0.0 0.02573787064084197 0.0 30-31 0.0 0.0 0.0 0.06057185620696142 0.0 32-33 0.0 0.0 0.0 0.12114371241392284 0.0 34-35 0.0 0.0 0.0 0.21045102258937448 0.0 36-37 0.0 0.0 0.0 0.3400706602745786 0.0 38-39 0.0 0.0 0.0 0.5372909702453676 0.0 40-41 0.0 0.0 0.0 0.7741000531295803 0.0 42-43 0.0 0.0 0.0 0.9803131130833143 0.0 44-45 0.0 0.0 0.0 1.2152616270055383 0.0 46-47 0.0 0.0 0.0 1.4869046954558702 0.0 48-49 0.0 0.0 0.0 1.7792207523727424 0.0 50-51 0.0 0.0 0.0 2.09820496441145 0.0 52-53 0.0 0.0 0.0 2.3882469925970025 0.0 54-55 0.0 0.0 0.0 2.695240871325118 0.0 56-57 0.0 0.0 0.0 3.049575893641609 0.0 58-59 0.0 0.0 0.0 3.4598313497600897 0.0 60-61 0.0 0.0 0.0 3.9026467627133714 0.0 62-63 0.0 0.0 0.0 4.331094448682407 0.0 64-65 0.0 0.0 0.0 4.76595076107607 0.0 66-67 0.0 0.0 0.0 5.232953570535203 0.0 68-69 0.0 0.0 0.0 5.745230224736058 0.0 70-71 0.0 0.0 0.0 6.32944887880047 0.0 72-73 0.0 0.0 0.0 6.92503767652148 0.0 74-75 0.0 0.0 0.0 7.552049416711631 0.0 76-77 0.0 0.0 0.0 8.171825610938491 0.0 78-79 0.0 0.0 0.0 8.861786601009255 0.0 80-81 0.0 0.0 0.0 9.659453860990691 0.0 82-83 0.0 0.0 0.0 10.419186187136027 0.0 84-85 0.0 0.0 0.0 11.204346289095206 0.0 86-87 0.0 0.0 0.0 12.004907767461956 0.0 88-89 0.0 0.0 0.0 12.856014702629398 0.0 90-91 0.0 0.0 0.0 13.803871223820245 0.0 92-93 0.0 0.0 0.0 14.833179319569258 0.0 94-95 0.0 0.0 0.0 15.850703811892345 0.0 96-97 0.0 0.0 0.0 16.894276269683267 0.0 98-99 0.0 0.0 0.0 17.950872710208117 0.0 100-101 0.0 0.0 0.0 19.115382150528298 0.0 102-103 0.0 0.0 0.0 20.35906913667533 0.0 104-105 0.0 0.0 0.0 21.607304180285 0.0 106-107 0.0 0.0 0.0 22.853988749759676 0.0 108-109 0.0 0.0 0.0 24.046716819336687 0.0 110-111 0.0 0.0 0.0 25.331853147359126 2.0672988466539734E-4 112-113 0.0 0.0 0.0 26.66743156723993 2.0672988466539734E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1280 0.0 92.08034 1 GTATCAA 3010 0.0 62.690895 1 CGGGGTA 15 0.0042357664 59.50207 44-45 GTATGGT 165 0.0 54.11516 1 ATTGTCA 35 0.0077797356 51.02287 1 GTAAGAG 35 0.0077797356 51.02287 3 TCAACGC 3830 0.0 49.1134 4 CAACGCA 3875 0.0 48.67653 5 AACGCAG 3915 0.0 48.179195 6 ATCAACG 4060 0.0 46.331116 3 TATCAAC 4095 0.0 45.935123 2 AAGACAT 150 1.8189894E-12 43.634853 5 GGTACTC 250 0.0 42.85921 3 GGTTTGC 70 5.4494783E-5 42.497086 8 ACGCAGA 4480 0.0 41.83307 7 CGCAGAG 4495 0.0 41.561108 8 GTGGTAC 260 0.0 41.21078 1 ACTCCCG 280 0.0 38.25133 6 GCAGAGT 4830 0.0 37.816246 9 TGGTACT 285 0.0 37.595802 2 >>END_MODULE