FastQCFastQC Report
Fri 27 May 2016
SRR940138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445889
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.063.6275441
GTATCAA40650.059.4538841
ATCAACG49150.048.203033
TCAACGC49950.047.311834
TATCAAC53350.045.1892782
CAACGCA52500.045.0101475
AACGCAG53600.044.1839266
GTACATG124300.037.4019851
TACATGG129950.034.6306232
ACATGGG127300.034.042213
ACGCAGA71150.032.9461867
CGCAGAG71850.032.54448
CATGGGG67200.031.0922834
TAAGGTG10900.027.8498525
GTAAGGT9250.027.6720584
GCAGAGT80250.027.5808969
GAGTACA92450.025.3046531
CATGGGA71400.025.0113894
AGGTAAG10300.024.8511222
ATGGGGA38600.024.826635
Produced by FastQC (version 0.11.5)