FastQCFastQC Report
Fri 27 May 2016
SRR940138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445889
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53310.217957560625196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41910.17134874068283557No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35250.074.695611
GTATCAA74700.067.385741
ATCAACG98400.050.5336953
TATCAAC99000.050.3508262
TCAACGC100150.049.650684
CAACGCA102850.048.4862375
AACGCAG104950.047.5708056
ACGCAGA130050.037.9691667
CGCAGAG131550.037.4457748
GCAGAGT140650.034.4731679
GTACATG125200.033.87721
TACATGG129000.031.8086952
ACATGGG127200.031.7418523
GGACCGA10800.029.7530176
GAGTACT78400.027.58364712-13
CATGGGG69150.027.5579854
AGAGTAC132850.027.33930410-11
AGTACTT82500.026.53732912-13
AGGACCG12650.026.343675
CAGAGTA141350.025.59004810-11
Produced by FastQC (version 0.11.5)