FastQCFastQC Report
Fri 27 May 2016
SRR940137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041217
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG22050.21177141748550013No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG21560.20706538598582236No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG20790.19967019362918584No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG17970.17258650214124432No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC16910.162406107468472No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG16330.15683570283620032No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC14890.1430057327146983No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC12790.1228370262875078No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC12480.11985974105301776No Hit
CCCCTGGGGCGCGCCAGTCTGCGCTGGTTGTGGCCCCGCCACCCTGCGGA12360.1187072435428926No Hit
AGTCTGCACCGTCTCTTCGCGGTTAGCTCCTACTCCGGATCAGCCATGAC12340.11851516062453839No Hit
GAACAGACCCGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAG12310.11822703624700712No Hit
GCTCTGGAGGGCGTAGGCCACTTCTTCCGCGAATTGGCCGAGGAGAAGCG10430.10017124192171277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.063.600071
GTATCAA17950.055.371121
GTACATG58700.045.7266851
TACATGG62250.042.449732
ATCAACG23000.042.1784933
TCAACGC23500.041.5343364
ACATGGG63200.040.3990823
TATCAAC24250.040.2497672
AACGCAG24400.039.750786
CAACGCA24750.039.434755
CATGGGG34850.037.0585334
ACTTGAC7100.034.3599478
CATGGGA29750.033.80894
ACGCAGA30100.031.8247857
AGTACAT40000.030.5017782
CGCAGAG32000.029.7506838
ATGGGGA18850.029.6774715
GAGTACA44500.029.2897321
ATGGGGG17850.028.0060275
ATGGGAG14950.027.0693685