FastQCFastQC Report
Fri 27 May 2016
SRR940137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041217
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44320.4256557470728965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37920.364189213199554No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG25270.24269676734052556No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG21630.20773767620006203No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG21330.2048564324247491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.20322372761873847No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC17870.17162608754947337No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG17190.16509526832543073No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG16090.15453070781595No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC15360.14751968129602186No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC12970.12456577255269555No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC12540.12043598980808035No Hit
AGTCTGCACCGTCTCTTCGCGGTTAGCTCCTACTCCGGATCAGCCATGAC11820.11352100474732933No Hit
CCCCTGGGGCGCGCCAGTCTGCGCTGGTTGTGGCCCCGCCACCCTGCGGA11640.11179225848214155No Hit
GCTCTGGAGGGCGTAGGCCACTTCTTCCGCGAATTGGCCGAGGAGAAGCG11470.11015955367613091No Hit
GAACAGACCCGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAG11310.10862289032929735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.076.797821
GTATCAA41450.060.6527331
GTGGTAT7400.054.7443921
TGGTATC8450.047.9326132
TATCAAC53300.047.3824582
ATCAACG53350.047.1147583
TCAACGC53900.046.855014
CAACGCA54100.046.4191865
AACGCAG56000.044.9505166
TAGCACG705.41751E-542.545234
GTACATG52600.039.1879731
ACGCAGA66000.037.9467437
CGCAGAG67350.037.186128
TACATGG56700.035.7169842
ACATGGG55250.035.684093
GCAGAGT73150.033.7497149
ATGGGAG12500.033.325025
CATGGGA27650.032.0974164
GAGTACT48400.028.14663312-13
CGTATCC850.007172718628.02983
Produced by FastQC (version 0.11.5)