FastQCFastQC Report
Fri 27 May 2016
SRR940136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262364
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA4940.18828802732082145No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA3790.14445579424006344No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA3770.1436934945343111No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA3210.12234910277324632No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG3190.12158680306749402No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG3090.11777530453873244No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG2870.109390007775457No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGC150.004237795759.4910462-63
GGTATCA2550.058.3690571
GTGTTCG350.007770750751.031231
GTCCGTA350.007770750751.031231
GTATCAA3850.047.9384271
ATCAACG4000.044.652333
CAACGCA4150.043.0383875
TCAACGC4200.042.5260244
AACGCAG4350.041.0361256
TACATGG11550.040.721892
GTACATG11750.040.5354461
TATCAAC4600.038.828112
ACATGGG12000.038.698683
GAGTACA9400.038.635351
CATGGGG7800.037.4010964
GTTCCGA650.001930363136.637811
AGTACAT9350.033.747932
ATGGGGG4150.031.5614855
GTAAGGT1152.7444035E-531.0624894
TAAGGTG1352.3104349E-630.8707435
Produced by FastQC (version 0.11.5)