##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940136_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 262364 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40437712491043 33.0 31.0 34.0 28.0 34.0 2 31.52102041438612 34.0 31.0 34.0 28.0 34.0 3 31.6302808312116 34.0 31.0 34.0 28.0 34.0 4 34.89422710432834 37.0 35.0 37.0 32.0 37.0 5 34.95439542010337 37.0 35.0 37.0 32.0 37.0 6 34.99268573432331 37.0 35.0 37.0 32.0 37.0 7 34.909187236053725 37.0 35.0 37.0 32.0 37.0 8 34.85414538579988 37.0 35.0 37.0 32.0 37.0 9 36.44674955405467 39.0 38.0 39.0 32.0 39.0 10-11 36.41233553383849 39.0 37.5 39.0 32.0 39.0 12-13 36.32074141269381 39.0 37.0 39.0 32.0 39.0 14-15 37.73987094265982 41.0 38.0 41.0 32.0 41.0 16-17 37.74106203595005 41.0 38.0 41.0 32.0 41.0 18-19 37.73422611333872 41.0 38.0 41.0 32.0 41.0 20-21 37.75831859553902 41.0 38.0 41.0 32.0 41.0 22-23 37.753744797304506 41.0 38.0 41.0 32.0 41.0 24-25 37.76017479532253 41.0 38.0 41.0 32.0 41.0 26-27 37.70531970849659 41.0 38.0 41.0 32.0 41.0 28-29 37.635563568172465 40.0 38.0 41.0 32.0 41.0 30-31 37.55635300574774 40.0 38.0 41.0 31.5 41.0 32-33 37.46435105426049 40.0 38.0 41.0 31.0 41.0 34-35 37.3785580338766 40.0 38.0 41.0 31.0 41.0 36-37 37.247796953850376 40.0 38.0 41.0 30.5 41.0 38-39 37.10825036971536 40.0 37.5 41.0 30.0 41.0 40-41 36.96750888079157 40.0 37.0 41.0 30.0 41.0 42-43 36.943608879266975 40.0 37.0 41.0 30.0 41.0 44-45 37.01996462929365 40.0 37.0 41.0 30.0 41.0 46-47 36.92231213123752 40.0 37.0 41.0 30.0 41.0 48-49 36.7959704837554 40.0 37.0 41.0 30.0 41.0 50-51 36.64232707231175 40.0 36.0 41.0 30.0 41.0 52-53 36.467579393514356 40.0 36.0 41.0 30.0 41.0 54-55 36.28110754524249 40.0 35.0 41.0 30.0 41.0 56-57 36.06563400466527 39.0 35.0 41.0 29.0 41.0 58-59 35.81606279824976 39.0 35.0 41.0 29.0 41.0 60-61 35.57118735802168 39.0 35.0 41.0 28.0 41.0 62-63 35.27659473098443 38.0 35.0 41.0 28.0 41.0 64-65 34.978554603527925 37.5 35.0 40.0 28.0 41.0 66-67 34.648661782866554 37.0 34.0 40.0 28.0 41.0 68-69 34.32587550121205 36.5 34.0 39.5 27.5 41.0 70-71 34.21910780442438 36.0 34.0 39.0 28.0 41.0 72-73 34.00516648625573 36.0 34.0 39.0 28.0 41.0 74-75 33.70326531078959 35.0 34.0 38.0 28.0 40.0 76-77 33.35989312558125 35.0 34.0 37.0 28.0 39.0 78-79 33.04222568645089 35.0 34.0 37.0 27.0 39.0 80-81 32.746762131999816 35.0 34.0 36.0 27.0 38.5 82-83 32.48273772316324 35.0 34.0 36.0 26.5 37.0 84-85 32.255757268527695 35.0 34.0 35.5 26.0 37.0 86-87 32.05700096049763 35.0 34.0 35.0 26.0 36.5 88-89 31.905259486819837 35.0 33.5 35.0 25.5 36.0 90-91 31.784042399109634 35.0 33.0 35.0 25.0 36.0 92-93 31.64223559634706 35.0 33.0 35.0 25.0 36.0 94-95 31.559874830388317 35.0 33.0 35.0 25.0 36.0 96-97 31.448022594563277 35.0 33.0 35.0 24.5 35.0 98-99 31.370441447759603 35.0 33.0 35.0 24.0 35.0 100-101 31.30707909621747 35.0 33.0 35.0 24.0 35.0 102-103 30.501463615435043 34.0 31.5 35.0 21.5 35.0 104-105 31.12046622250004 35.0 33.0 35.0 23.0 35.0 106-107 31.199105060145445 35.0 33.0 35.0 24.0 35.0 108-109 31.159177707307407 35.0 33.0 35.0 23.0 35.0 110-111 31.070249729383605 35.0 33.0 35.0 21.5 35.0 112-113 30.980631870226098 35.0 33.0 35.0 20.0 35.0 114-115 30.860287615678978 35.0 33.0 35.0 19.0 35.0 116-117 30.788433245414765 35.0 33.0 35.0 18.5 35.0 118-119 30.67640568065741 35.0 33.0 35.0 16.0 35.0 120-121 30.539464255766795 35.0 33.0 35.0 4.5 35.0 122-123 30.317621701148024 35.0 33.0 35.0 2.0 35.0 124-125 28.29695956762361 33.0 29.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43377805301900096 1101 2 0.5165172625399599 1101 3 0.3635233500096682 1101 4 0.5748878930206942 1101 5 0.33959903472840836 1101 6 0.48875783284888286 1101 7 0.39771574173366986 1101 8 0.5367008476641288 1101 9 0.6474274459454676 1101 10-11 0.4708320677959392 1101 12-13 0.49232933969236115 1101 14-15 0.6870326817668868 1101 16-17 0.4713056622575067 1101 18-19 0.6798574080991173 1101 20-21 0.7183437243200927 1101 22-23 0.6950590017260225 1101 24-25 0.643472245045686 1101 26-27 0.7205500350726268 1101 28-29 0.5636894252025328 1101 30-31 0.6941023673114515 1101 32-33 0.5958328726619229 1101 34-35 0.43708326210312265 1101 36-37 0.4751947225627333 1101 38-39 0.5281330457977731 1101 40-41 0.21188900581963566 1101 42-43 0.44053904137338407 1101 44-45 0.5776990981537793 1101 46-47 0.686167206917311 1101 48-49 0.4909059249523082 1101 50-51 0.31676625862852603 1101 52-53 0.19974134420550627 1101 54-55 0.16022246525625405 1101 56-57 0.14795708687535836 1101 58-59 0.09465875558896641 1101 60-61 0.05446810140371383 1101 62-63 0.08598070807195057 1101 64-65 -0.02799121832802598 1101 66-67 -0.01075877789003954 1101 68-69 -0.2777830421090286 1101 70-71 -0.0656780412680078 1101 72-73 0.16046776845981725 1101 74-75 0.3142051268264794 1101 76-77 0.3504782295963338 1101 78-79 0.30176933069356693 1101 80-81 0.18020361974698318 1101 82-83 0.11903953750218221 1101 84-85 0.25807169666424556 1101 86-87 0.29612307835391505 1101 88-89 0.14019079750649865 1101 90-91 0.24987653083417882 1101 92-93 -0.1367499630108142 1101 94-95 0.08582247140472177 1101 96-97 0.06787667803699549 1101 98-99 -0.005918930333532302 1101 100-101 0.17127244958020427 1101 102-103 0.17023601727537852 1101 104-105 0.16847982472502565 1101 106-107 0.2920914039572473 1101 108-109 0.3186643396567277 1101 110-111 0.25912147367743543 1101 112-113 0.06635306167567734 1101 114-115 -0.13316310911967122 1101 116-117 -0.1261647454228374 1101 118-119 -0.3017039522806009 1101 120-121 -0.34628894836620105 1101 122-123 -0.5490616447481464 1101 124-125 -0.5104413880744545 1102 1 -0.6127974495522928 1102 2 -0.381507515393654 1102 3 -0.3487253643428332 1102 4 -0.4917320228278328 1102 5 -0.45382078761848277 1102 6 -0.36835997921283337 1102 7 -0.505656161024632 1102 8 -0.3815728783760761 1102 9 -0.471436509173742 1102 10-11 -0.40771807245651814 1102 12-13 -0.35451790200937694 1102 14-15 -0.580849506171397 1102 16-17 -0.3465122433058454 1102 18-19 -0.3981434100402055 1102 20-21 -0.509376588910385 1102 22-23 -0.2948895723740179 1102 24-25 -0.45087674981171944 1102 26-27 -0.2541168653481307 1102 28-29 -0.23662614281990813 1102 30-31 -0.3431814101546564 1102 32-33 -0.33737189592152106 1102 34-35 -0.3469571773545681 1102 36-37 -0.398884870705146 1102 38-39 -0.4812463370914344 1102 40-41 -0.20920437893960298 1102 42-43 -0.12497054254433237 1102 44-45 -0.2548307054927861 1102 46-47 -0.0968359487629229 1102 48-49 -0.08293114844646965 1102 50-51 -0.43368663992219325 1102 52-53 -0.28649638370472985 1102 54-55 -0.07909789472505224 1102 56-57 -0.2957958350507752 1102 58-59 -0.27902637765086524 1102 60-61 -0.012224862598152697 1102 62-63 -0.12699848781397804 1102 64-65 -0.11168979242806643 1102 66-67 -0.034806229970449465 1102 68-69 -0.17560328521329183 1102 70-71 -0.10598601228250715 1102 72-73 -0.027762713073613554 1102 74-75 -0.12805761277146388 1102 76-77 -0.09536386951540265 1102 78-79 -0.18040691989082092 1102 80-81 -0.17235598754614756 1102 82-83 -0.3066120520256348 1102 84-85 -0.30080511492996465 1102 86-87 -0.16409489733566218 1102 88-89 -0.22275625719195347 1102 90-91 0.009489667814079894 1102 92-93 -0.035622099523202166 1102 94-95 0.06853927832384343 1102 96-97 -0.14285263472597975 1102 98-99 0.03114570267254635 1102 100-101 0.1059381812305098 1102 102-103 0.10284179100142055 1102 104-105 0.11443880088210534 1102 106-107 0.018160828922187733 1102 108-109 -0.058702420511572484 1102 110-111 -0.013128993830743951 1102 112-113 -0.13948202481333283 1102 114-115 0.05958174829033425 1102 116-117 0.012144628116200096 1102 118-119 0.24189118563431222 1102 120-121 0.21283622694937065 1102 122-123 0.15182077694423057 1102 124-125 0.24671733095313186 1103 1 0.18874280037505287 1103 2 0.28129399579495384 1103 3 0.28790642874057326 1103 4 0.04545193532386094 1103 5 0.019123124035111516 1103 6 0.05382833813677479 1103 7 -0.3334387823556426 1103 8 -0.12491783623716657 1103 9 0.30929583607473177 1103 10-11 0.31769605134469714 1103 12-13 0.32877910467473015 1103 14-15 0.40507028458709726 1103 16-17 0.11626747189323083 1103 18-19 0.31564618600745575 1103 20-21 0.17008432361504333 1103 22-23 0.03862539420546085 1103 24-25 0.42374545303628963 1103 26-27 0.22517694459083515 1103 28-29 0.15731457208855204 1103 30-31 0.5604948467474102 1103 32-33 0.3280596646713292 1103 34-35 0.4719980682135798 1103 36-37 0.31214390000103975 1103 38-39 0.012607193858876542 1103 40-41 -0.24709307408635084 1103 42-43 -0.004554965677144196 1103 44-45 -0.11446453286854563 1103 46-47 -0.02501816323545114 1103 48-49 -0.006347306540050113 1103 50-51 -0.0584614146969713 1103 52-53 -0.08455654063585882 1103 54-55 -0.21076020219381064 1103 56-57 -0.029848436038861337 1103 58-59 0.1399143372575935 1103 60-61 -0.09865322644587593 1103 62-63 -0.10351694175178494 1103 64-65 -0.13695860963237294 1103 66-67 0.014314665118185133 1103 68-69 0.08574516000612675 1103 70-71 0.16338335708686458 1103 72-73 0.13678974260787413 1103 74-75 0.026337911785354606 1103 76-77 -0.11283698779497087 1103 78-79 0.09721586418357475 1103 80-81 0.007700682026644756 1103 82-83 0.12127954925306739 1103 84-85 0.17442011029525872 1103 86-87 -0.02306757969544293 1103 88-89 0.07530389973917195 1103 90-91 0.3716009726673164 1103 92-93 0.23233053052620534 1103 94-95 0.2852422716397385 1103 96-97 -0.09684130081141973 1103 98-99 0.3167676149073593 1103 100-101 -0.03792261504964145 1103 102-103 0.08568108190522494 1103 104-105 0.34671866138777574 1103 106-107 0.374656033804488 1103 108-109 0.3201772656261781 1103 110-111 0.23982799541656874 1103 112-113 0.20544677495417574 1103 114-115 0.4103518732540081 1103 116-117 0.30012050722111283 1103 118-119 0.49791414407662415 1103 120-121 0.5381631667924367 1103 122-123 0.30530086994045647 1103 124-125 0.6924816436529255 1104 1 0.13276029266840794 1104 2 0.2774012436263398 1104 3 0.1913690425480148 1104 4 0.12923571910764764 1104 5 0.3061974662818656 1104 6 0.32388353922645763 1104 7 0.36224549608726164 1104 8 0.338835837861339 1104 9 0.2827347922723078 1104 10-11 0.15923666357496558 1104 12-13 0.07903718769143353 1104 14-15 -0.1517402394648002 1104 16-17 -0.13118395831681795 1104 18-19 0.08062216994895977 1104 20-21 0.05693284322218517 1104 22-23 0.14078325498401512 1104 24-25 0.03844543152936808 1104 26-27 -0.087264807506088 1104 28-29 0.12704716940735494 1104 30-31 0.07485429903789509 1104 32-33 -0.013828642734225127 1104 34-35 -0.02078636077774121 1104 36-37 0.2948774292862879 1104 38-39 0.18834380580238275 1104 40-41 0.24383817546558362 1104 42-43 0.23209999166746798 1104 44-45 0.1653583935061036 1104 46-47 0.09830608939913787 1104 48-49 0.180092581623974 1104 50-51 0.4208668525623267 1104 52-53 0.08739126924299256 1104 54-55 0.16039185171686654 1104 56-57 0.1935905201587147 1104 58-59 -0.08014086383208507 1104 60-61 0.030072847107781797 1104 62-63 0.14231071614483426 1104 64-65 0.16781592617664387 1104 66-67 -0.11686678158050512 1104 68-69 0.13209615290884358 1104 70-71 -0.05138831113307418 1104 72-73 0.024727946063322293 1104 74-75 0.08595867313024996 1104 76-77 0.017799616979566224 1104 78-79 0.09465654093460074 1104 80-81 -0.1864207603706589 1104 82-83 -0.09255298354498365 1104 84-85 0.1074619054060264 1104 86-87 0.24916698909085255 1104 88-89 0.21038526757916287 1104 90-91 0.14157731505745375 1104 92-93 0.08279651375080732 1104 94-95 0.21455978544001297 1104 96-97 0.1149444902847172 1104 98-99 0.26403623631391326 1104 100-101 0.26793085114864823 1104 102-103 -0.20319625697211663 1104 104-105 0.05181580096767391 1104 106-107 -0.09857143769884402 1104 108-109 -0.12889793689729956 1104 110-111 0.20773250470038462 1104 112-113 0.09908789618150493 1104 114-115 0.12732083160227603 1104 116-117 0.1610101767314731 1104 118-119 -0.09583092725465292 1104 120-121 -0.3254327899069658 1104 122-123 -0.2221025281525968 1104 124-125 -0.2753834362811176 1105 1 -0.08391389887739109 1105 2 -0.07691474486059491 1105 3 -0.18943687200698278 1105 4 -0.4026791745093732 1105 5 -0.1372742307665007 1105 6 -0.010432731443444254 1105 7 -0.08395632117105123 1105 8 -0.3840322698196843 1105 9 -0.24468633758829128 1105 10-11 -0.25959693312913146 1105 12-13 -0.1261662662280898 1105 14-15 -0.2432980305365362 1105 16-17 -0.011291470027408934 1105 18-19 -0.2231548490701698 1105 20-21 0.036365232183186436 1105 22-23 0.09925650403869923 1105 24-25 0.049866787136352286 1105 26-27 0.16429425338899506 1105 28-29 -0.0052860604588857996 1105 30-31 0.11633872253809585 1105 32-33 0.08837450078403464 1105 34-35 0.14765217977194567 1105 36-37 0.048824463543297725 1105 38-39 0.1438823519957353 1105 40-41 0.13265103172157922 1105 42-43 0.2643711412808756 1105 44-45 0.2707784228531267 1105 46-47 0.1447992605713324 1105 48-49 -0.09934615645379807 1105 50-51 0.24938073596432986 1105 52-53 0.22697307408525091 1105 54-55 -0.026092289600363472 1105 56-57 0.10100981583002522 1105 58-59 -0.005133103800488925 1105 60-61 -0.10542435928083904 1105 62-63 -0.06948876463850695 1105 64-65 -0.11647988622812022 1105 66-67 -0.02509735328320062 1105 68-69 0.13184924281233634 1105 70-71 0.17770193097990727 1105 72-73 0.10317128372346218 1105 74-75 0.09866396123906185 1105 76-77 0.19434386326886255 1105 78-79 0.38490994123934996 1105 80-81 0.2312558861669558 1105 82-83 -0.009111480074132317 1105 84-85 -0.09779093053279553 1105 86-87 -0.1318082352446055 1105 88-89 -0.3598959277478926 1105 90-91 0.053472738049723745 1105 92-93 -0.05499695653532655 1105 94-95 -0.10795065532978043 1105 96-97 0.040529314484729184 1105 98-99 -0.02300380544916436 1105 100-101 0.07390889731378536 1105 102-103 0.2004453142226481 1105 104-105 -0.1113247543684075 1105 106-107 0.14500537250489032 1105 108-109 0.1796726652811529 1105 110-111 0.30684927201231105 1105 112-113 0.26145597564422474 1105 114-115 0.421097991712891 1105 116-117 0.40150061072887766 1105 118-119 0.33243110784890817 1105 120-121 0.4768204410880088 1105 122-123 0.5266205939197555 1105 124-125 0.6539896942567225 1106 1 0.5417299980990933 1106 2 0.26337080945071634 1106 3 0.2612350204902185 1106 4 0.5029199296339613 1106 5 0.31997660909912184 1106 6 0.38995920584659416 1106 7 0.5709708904750883 1106 8 0.6451390627671003 1106 9 0.5457111988058827 1106 10-11 0.6538269190545165 1106 12-13 0.5131674403788509 1106 14-15 0.45556815087444136 1106 16-17 0.6550299185504187 1106 18-19 0.5415707948268036 1106 20-21 0.5706177517800484 1106 22-23 0.7377363472637839 1106 24-25 0.7426999338328741 1106 26-27 0.7436335591000827 1106 28-29 0.409341599115578 1106 30-31 0.4951321155952044 1106 32-33 0.605157815453687 1106 34-35 0.4502697609589532 1106 36-37 0.368687285491788 1106 38-39 0.41637675289159404 1106 40-41 0.5936252758425198 1106 42-43 0.7560424738676659 1106 44-45 0.4747957800759721 1106 46-47 0.5642421497090666 1106 48-49 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-0.9799551123894261 2216 88-89 -0.8830385152564695 2216 90-91 -0.7139646332611918 2216 92-93 -0.8366973683404062 2216 94-95 -0.6797806141492728 2216 96-97 -0.6317639250485598 2216 98-99 -0.647617738187666 2216 100-101 -0.6900967650060501 2216 102-103 -0.6216205745899792 2216 104-105 -0.7565289410533751 2216 106-107 -0.823573042045556 2216 108-109 -0.7240250011567326 2216 110-111 -0.7658171658737558 2216 112-113 -0.8232857882541964 2216 114-115 -0.7411574990214902 2216 116-117 -0.7956510708085283 2216 118-119 -0.6092512874839677 2216 120-121 -0.7508734504699852 2216 122-123 -0.8226415886471621 2216 124-125 -1.2620192281866522 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4845.0 3 910.0 4 636.0 5 476.0 6 450.0 7 481.0 8 513.0 9 578.0 10 645.0 11 713.0 12 754.0 13 858.0 14 824.0 15 791.0 16 751.0 17 733.0 18 667.0 19 624.0 20 658.0 21 661.0 22 827.0 23 1009.0 24 1356.0 25 1858.0 26 2416.0 27 2436.0 28 2774.0 29 3557.0 30 4091.0 31 4746.0 32 6073.0 33 8025.0 34 11989.0 35 19035.0 36 42193.0 37 88758.0 38 43348.0 39 305.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.54379109882779 17.26369960926332 11.30734775564662 24.88516153626227 2 15.956789888162962 19.262729372660534 38.674879738970674 26.105601000205837 3 20.225502864526753 22.936416273103944 28.386068832500467 28.45201202986884 4 12.539219610758467 14.792321915327577 35.62189054726368 37.046567926650276 5 13.283468859483591 39.09748352146879 33.61201017113274 14.00703744791488 6 34.254529096010735 35.031108942295965 16.349350372087347 14.365011589605952 7 30.588665810268395 32.506794643571865 18.71967187494044 18.1848676712193 8 28.354169842963866 35.17799969507547 18.15444427504193 18.313386186918738 9 26.84610575750183 16.281028909490118 18.39930470846548 38.47356062454257 10-11 26.617028250827097 26.048733820188748 25.915140796755654 21.419097132228508 12-13 26.268083833134177 23.26622947195493 26.627902323001894 23.837784371909002 14-15 22.434851557615772 24.96531007456428 23.8765801070432 28.723258260776753 16-17 23.65814148498247 28.988412867815217 24.516694618082024 22.836751029120293 18-19 23.55070184968124 27.33506770729104 26.01065401145449 23.10357643157323 20-21 23.629935965848453 26.77370788229913 26.29097423387712 23.3053819179753 22-23 23.57711182047739 26.65319929486874 26.211825241793317 23.557863642860546 24-25 23.233586116071685 26.955953290781466 26.354839877795204 23.455620715351643 26-27 23.1136893781159 27.476140019209954 25.932483115061515 23.47768748761263 28-29 23.00667047836859 27.572136458928913 26.125023823137028 23.29616923956547 30-31 22.924154701691933 27.084040051379194 26.257322869459486 23.734482377469384 32-33 22.705607699271045 27.299061413121155 26.36314259850398 23.632188289103816 34-35 23.543626412160204 27.23334756292784 25.894749279626776 23.328276745285176 36-37 23.585747121179203 26.84421777264463 25.80932886596759 23.760706240208577 38-39 23.149986850279955 26.993897767596803 26.084661328007382 23.77145405411586 40-41 23.346694341679676 26.77805287604758 26.360068691974437 23.51518409029831 42-43 23.27495678458234 26.722470301907574 26.354829549244613 23.647743364265473 44-45 22.930852776999586 27.02712489113262 26.487790654462085 23.554231677405706 46-47 23.26820705467977 27.37171533507537 25.778913723404862 23.58116388683999 48-49 23.397844544181126 27.258225894056775 26.00954804036477 23.33438152139733 50-51 23.296790842522956 27.054085681722018 26.197573405808427 23.4515500699466 52-53 23.600835515507733 26.927650913082758 26.21849187926191 23.253021692147602 54-55 23.081614295079966 26.952118495479425 26.375230602692522 23.591036606748084 56-57 23.16595987992567 27.441559060370707 26.2000285891266 23.192452470577024 58-59 23.447608083226275 26.849737535309792 26.065583769503775 23.637070611960155 60-61 22.87266684199894 26.99469048136332 26.803349608742224 23.329293067895517 62-63 22.937722389408023 27.217890638251646 26.634317961345456 23.210069010994875 64-65 22.74730950207445 27.224008370226237 26.567463985348294 23.46121814235102 66-67 22.707773112847065 27.44173548127665 26.264915126000776 23.585576279875514 68-69 22.937598146087115 26.87659130063576 26.649616563248006 23.53619399002912 70-71 22.798143718851545 27.032523036753986 26.578363080206973 23.590970164187496 72-73 22.81300562006529 27.12496212316119 26.581822547724816 23.480209709048705 74-75 22.40931432807887 27.50368307804314 26.578768317714797 23.508234276163193 76-77 22.945482892763135 27.03288409087011 26.364643074289873 23.656989942076883 78-79 23.14991452567946 27.07125146983433 26.513238514328624 23.26559549015759 80-81 23.13730796571827 27.62415882266619 26.391289681367457 22.84724353024808 82-83 22.909464440217867 27.475691890181846 26.462202842648107 23.152640826952176 84-85 22.921971002119193 27.44488573127411 26.357846350871313 23.275296915735392 86-87 22.80420567961137 27.287938877761242 26.58756401045131 23.32029143217608 88-89 22.796575606148966 27.5243660259269 26.091373073683165 23.587685294240966 90-91 22.7532961566756 27.687334906289667 26.47235954884868 23.087009388186054 92-93 22.716486582816017 26.915181745234563 26.779094390113272 23.58923728183615 94-95 22.682799469439406 26.95796679422482 26.947104023417843 23.41212971291793 96-97 22.94050048882562 26.856250964787403 26.812037497593987 23.391211048792993 98-99 22.862696892598837 26.90241369124191 26.41262399588348 23.82226542027577 100-101 22.45811163116891 26.97054473936973 26.96349346709152 23.607850162369836 102-103 22.5581634675489 26.49753777195042 26.952821271210993 23.99147748928969 104-105 22.25724806172002 26.86833418462641 26.991072857981447 23.883344895672128 106-107 22.677844521352014 27.029622966565537 26.528982634812703 23.763549877269746 108-109 22.873946120656797 26.742235977496915 26.287333628089222 24.09648427375707 110-111 22.594606338152982 26.819851084468688 26.7523874319408 23.83315514543753 112-113 22.233614758628576 27.23885574888986 26.93125726591832 23.596272226563244 114-115 22.18010436081857 27.11170571621328 26.888157067224682 23.820032855743467 116-117 22.20020010481681 26.95888322454619 27.01910524560484 23.82181142503216 118-119 22.178515206610943 27.372471441149905 26.284624151429565 24.164389200809598 120-121 22.324648000060986 27.431601094687412 26.552840012502 23.6909108927496 122-123 22.24581116311689 27.208001097711577 26.69306764647589 23.853120092695647 124-125 22.263247732842554 27.452106000934517 26.288727626422038 23.99591863980089 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 192.0 1 202.0 2 211.0 3 181.5 4 130.5 5 86.5 6 75.5 7 74.5 8 72.5 9 78.0 10 74.5 11 69.0 12 69.5 13 74.5 14 72.5 15 74.0 16 80.5 17 80.0 18 103.0 19 141.5 20 183.0 21 265.5 22 351.0 23 450.0 24 589.5 25 792.5 26 1040.5 27 1333.5 28 1640.0 29 1948.0 30 2462.5 31 3002.0 32 3695.0 33 4014.0 34 4076.0 35 4450.0 36 4999.0 37 5544.0 38 6017.5 39 6376.0 40 6602.5 41 7306.0 42 8102.5 43 8820.5 44 9242.5 45 9688.5 46 9948.0 47 9895.5 48 10105.0 49 10085.5 50 9969.0 51 9684.0 52 9577.0 53 9762.5 54 9827.0 55 9289.5 56 8291.0 57 7759.0 58 7597.0 59 7086.5 60 5962.0 61 4816.5 62 3996.0 63 3145.0 64 2449.0 65 2023.5 66 1550.5 67 1310.5 68 967.5 69 548.5 70 393.0 71 316.5 72 273.5 73 196.0 74 146.0 75 111.5 76 71.5 77 47.0 78 33.5 79 21.0 80 14.0 81 13.0 82 10.5 83 9.0 84 6.0 85 4.0 86 3.0 87 1.0 88 1.5 89 1.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014864844262170114 2 0.006860697351770822 3 0.006479547498894666 4 0.02248784131969325 5 0.02020094220243631 6 0.022868991172569405 7 0.00952874632190392 8 0.001524599411504627 9 0.010672195880532389 10-11 0.0 12-13 0.0036209236023234895 14-15 0.015245994115046272 16-17 0.001524599411504627 18-19 0.00819472183683737 20-21 0.001524599411504627 22-23 5.717247793142351E-4 24-25 0.0066701224253327435 26-27 0.0 28-29 0.0053360979402661945 30-31 3.8114985287615675E-4 32-33 5.717247793142351E-4 34-35 0.0 36-37 0.006479547498894666 38-39 3.8114985287615675E-4 40-41 0.012768520071351253 42-43 0.00590782271958043 44-45 0.0013340244850665486 46-47 0.010481620954094311 48-49 0.002477474043695019 50-51 0.008004146910399293 52-53 0.004192648381637724 54-55 0.003049198823009254 56-57 0.010100471101218155 58-59 0.01638944367367474 60-61 0.001905749264380784 62-63 0.005526672866704273 64-65 0.002096324190818862 66-67 0.0017151743379427056 68-69 0.0 70-71 0.004383223308075803 72-73 1.9057492643807837E-4 74-75 0.00590782271958043 76-77 0.012768520071351253 78-79 0.0017151743379427056 80-81 0.003239773749447333 82-83 0.0011434495586284702 84-85 0.0 86-87 0.0017151743379427056 88-89 0.004192648381637724 90-91 0.004954948087390038 92-93 0.012387370218475096 94-95 0.0 96-97 1.9057492643807837E-4 98-99 0.002477474043695019 100-101 0.0 102-103 0.0 104-105 0.006860697351770822 106-107 0.0 108-109 0.0 110-111 1.9057492643807837E-4 112-113 0.003430348675885411 114-115 0.001905749264380784 116-117 5.717247793142351E-4 118-119 0.004192648381637724 120-121 0.0022868991172569405 122-123 0.0 124-125 0.07489594609016481 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 262364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.99171996546984 #Duplication Level Percentage of deduplicated Percentage of total 1 82.53460571122895 53.6406598184466 2 10.304896394225917 13.394658814534214 3 3.006002909267672 5.860958978835367 4 1.32217792832446 3.4372247064875343 5 0.7266888494072805 2.361437910135373 6 0.43548915163301105 1.698191339455961 7 0.30324494223245324 1.3795887256561676 8 0.22301137754319333 1.1595114398720714 9 0.17219539470097328 1.0072147384574286 >10 0.8924338745012664 10.525521445966014 >50 0.05459683277733335 2.4117279789957204 >100 0.02465663415750538 3.1233041031575652 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 494 0.18828802732082145 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 379 0.14445579424006344 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 377 0.1436934945343111 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 321 0.12234910277324632 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 319 0.12158680306749402 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 309 0.11777530453873244 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 287 0.109390007775457 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.811498528761568E-4 2 0.0 0.0 0.0 0.0 3.811498528761568E-4 3 0.0 0.0 0.0 0.0 3.811498528761568E-4 4 0.0 0.0 0.0 0.0 3.811498528761568E-4 5 0.0 0.0 0.0 0.0 3.811498528761568E-4 6 0.0 0.0 0.0 0.0 3.811498528761568E-4 7 0.0 0.0 0.0 0.0 3.811498528761568E-4 8 0.0 0.0 0.0 0.0 3.811498528761568E-4 9 0.0 0.0 0.0 0.0 3.811498528761568E-4 10-11 0.0 0.0 0.0 0.0 3.811498528761568E-4 12-13 0.0 0.0 0.0 0.0 3.811498528761568E-4 14-15 0.0 0.0 0.0 0.0 3.811498528761568E-4 16-17 0.0 0.0 0.0 0.0 3.811498528761568E-4 18-19 0.0 0.0 0.0 0.0 3.811498528761568E-4 20-21 0.0 0.0 0.0 0.0 3.811498528761568E-4 22-23 0.0 0.0 0.0 0.0 3.811498528761568E-4 24-25 0.0 0.0 0.0 3.811498528761568E-4 3.811498528761568E-4 26-27 0.0 0.0 0.0 3.811498528761568E-4 3.811498528761568E-4 28-29 0.0 0.0 0.0 0.0017151743379427058 3.811498528761568E-4 30-31 0.0 0.0 0.0 0.007813571983961215 3.811498528761568E-4 32-33 0.0 0.0 0.0 0.013721394703541645 3.811498528761568E-4 34-35 0.0 0.0 0.0 0.02020094220243631 3.811498528761568E-4 36-37 0.0 0.0 0.0 0.03182601271515909 3.811498528761568E-4 38-39 0.0 0.0 0.0 0.054313854034852345 3.811498528761568E-4 40-41 0.0 0.0 0.0 0.07603939564879328 3.811498528761568E-4 42-43 0.0 0.0 0.0 0.09738378740985806 3.811498528761568E-4 44-45 0.0 0.0 0.0 0.12844750041926484 5.717247793142352E-4 46-47 0.0 0.0 0.0 0.16599076092756626 7.622997057523136E-4 48-49 0.0 0.0 0.0 0.19476757481971613 7.622997057523136E-4 50-51 0.0 0.0 0.0 0.24203015657635957 7.622997057523136E-4 52-53 0.0 0.0 0.0 0.29253251208245035 7.622997057523136E-4 54-55 0.0 0.0 0.0 0.34379716729429344 7.622997057523136E-4 56-57 0.0 0.0 0.0 0.40840206735680196 7.622997057523136E-4 58-59 0.0 0.0 0.0 0.4701483435227394 7.622997057523136E-4 60-61 0.0 0.0 0.0 0.5368495677760668 7.622997057523136E-4 62-63 0.0 0.0 0.0 0.6010733179856993 7.622997057523136E-4 64-65 0.0 0.0 0.0 0.668155692091903 7.622997057523136E-4 66-67 0.0 0.0 0.0 0.7565824579591712 7.622997057523136E-4 68-69 0.0 0.0 0.0 0.8684499397783232 7.622997057523136E-4 70-71 0.0 0.0 0.0 0.9988031894619689 7.622997057523136E-4 72-73 0.0 0.0 0.0 1.1276318397341099 7.622997057523136E-4 74-75 0.0 0.0 0.0 1.2461694439785946 7.622997057523136E-4 76-77 0.0 0.0 0.0 1.3894817886600297 7.622997057523136E-4 78-79 0.0 0.0 0.0 1.5476589776036347 7.622997057523136E-4 80-81 3.811498528761568E-4 0.0 0.0 1.7443323016877317 7.622997057523136E-4 82-83 3.811498528761568E-4 0.0 0.0 1.917946059672821 7.622997057523136E-4 84-85 3.811498528761568E-4 0.0 0.0 2.0980393651568052 7.622997057523136E-4 86-87 3.811498528761568E-4 0.0 0.0 2.2907106157857022 7.622997057523136E-4 88-89 3.811498528761568E-4 0.0 0.0 2.5214968517022154 7.622997057523136E-4 90-91 3.811498528761568E-4 0.0 0.0 2.7812504764373163 7.622997057523136E-4 92-93 3.811498528761568E-4 0.0 0.0 3.060061593816225 0.0011434495586284705 94-95 3.811498528761568E-4 0.0 0.0 3.3291533899467916 0.0011434495586284705 96-97 3.811498528761568E-4 0.0 0.0 3.5944336875485963 0.0011434495586284705 98-99 3.811498528761568E-4 0.0 0.0 3.901831043893217 0.0011434495586284705 100-101 5.717247793142352E-4 0.0 0.0 4.2688783522129565 0.0011434495586284705 102-103 7.622997057523136E-4 0.0 0.0 4.664321324571969 0.0011434495586284705 104-105 0.0011434495586284705 0.0 0.0 5.038991629949231 0.0011434495586284705 106-107 0.0011434495586284705 0.0 0.0 5.401846289887333 0.0011434495586284705 108-109 0.0011434495586284705 0.0 0.0 5.796336387614154 0.0011434495586284705 110-111 0.0011434495586284705 0.0 0.0 6.276585202238112 0.0011434495586284705 112-113 0.0011434495586284705 0.0 0.0 6.779703008034639 0.0011434495586284705 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCGC 15 0.0042377957 59.49104 62-63 GGTATCA 255 0.0 58.369057 1 GTGTTCG 35 0.0077707507 51.03123 1 GTCCGTA 35 0.0077707507 51.03123 1 GTATCAA 385 0.0 47.938427 1 ATCAACG 400 0.0 44.65233 3 CAACGCA 415 0.0 43.038387 5 TCAACGC 420 0.0 42.526024 4 AACGCAG 435 0.0 41.036125 6 TACATGG 1155 0.0 40.72189 2 GTACATG 1175 0.0 40.535446 1 TATCAAC 460 0.0 38.82811 2 ACATGGG 1200 0.0 38.69868 3 GAGTACA 940 0.0 38.63535 1 CATGGGG 780 0.0 37.401096 4 GTTCCGA 65 0.0019303631 36.63781 1 AGTACAT 935 0.0 33.74793 2 ATGGGGG 415 0.0 31.561485 5 GTAAGGT 115 2.7444035E-5 31.062489 4 TAAGGTG 135 2.3104349E-6 30.870743 5 >>END_MODULE