FastQCFastQC Report
Fri 27 May 2016
SRR940136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262364
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.4394657803662088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.3983015962555838No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA5130.1955298745254684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.1844765287920599No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA3830.14598039365156806No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA3160.12044335350886555No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA3120.11891875409736091No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG2930.11167690689271395No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG2900.11053345733408547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.10977115762833316No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG2830.10786540836395238No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.10291046027656234No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3950.082.985131
GTATCAA9050.064.537511
CAGCGTA150.00422516559.535904118-119
ATCAACG11000.052.534913
CAACGCA11000.052.484835
TCAACGC11050.052.297194
AACGCAG11250.051.3087126
TATCAAC11600.049.8175852
GCTAGAC558.463216E-443.327763
TGGTATC1700.042.0534132
ACGCAGA13950.041.7966047
CGCAGAG14150.041.205848
GTATAGA905.082218E-639.7322771
CTATAGT905.0955696E-639.717114
GTGGTAT2500.038.1429861
GCAGAGT15350.037.9845359
GTAAGGT650.001924129736.661952
CAGTCCG650.001936786336.6130379
ACATGGG11400.033.4459883
GTACATG11250.033.3751141
Produced by FastQC (version 0.11.5)