##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940136_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 262364 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70754371788812 34.0 31.0 34.0 31.0 34.0 2 32.7554199509079 34.0 31.0 34.0 31.0 34.0 3 32.840980469881536 34.0 31.0 34.0 31.0 34.0 4 36.257238035706116 37.0 37.0 37.0 35.0 37.0 5 36.21143525788599 37.0 37.0 37.0 35.0 37.0 6 36.27950862160967 37.0 37.0 37.0 35.0 37.0 7 36.25160845237914 37.0 37.0 37.0 35.0 37.0 8 36.23015352716074 37.0 37.0 37.0 35.0 37.0 9 38.03727264411276 39.0 39.0 39.0 35.0 39.0 10-11 38.01520597338049 39.0 39.0 39.0 35.0 39.0 12-13 38.00855681419707 39.0 39.0 39.0 35.0 39.0 14-15 39.51290192251986 41.0 39.0 41.0 36.0 41.0 16-17 39.49693174368434 41.0 39.0 41.0 36.0 41.0 18-19 39.4999676022625 41.0 39.5 41.0 36.0 41.0 20-21 39.484830235855526 41.0 39.5 41.0 36.0 41.0 22-23 39.464013736640695 41.0 39.0 41.0 36.0 41.0 24-25 39.446861993261265 41.0 39.0 41.0 36.0 41.0 26-27 39.334889695232576 41.0 39.0 41.0 36.0 41.0 28-29 39.22870896921833 41.0 39.0 41.0 35.5 41.0 30-31 39.08912236434877 41.0 39.0 41.0 35.0 41.0 32-33 38.92309158268665 41.0 38.0 41.0 35.0 41.0 34-35 38.80714960894025 40.5 38.0 41.0 35.0 41.0 36-37 38.68664717720419 40.0 38.0 41.0 35.0 41.0 38-39 38.51738424478968 40.0 38.0 41.0 34.5 41.0 40-41 38.38915590553582 40.0 38.0 41.0 34.0 41.0 42-43 38.19669619307527 40.0 38.0 41.0 34.0 41.0 44-45 38.28081405985577 40.0 38.0 41.0 34.0 41.0 46-47 38.10017761583144 40.0 38.0 41.0 33.5 41.0 48-49 37.85111905596804 40.0 38.0 41.0 33.0 41.0 50-51 37.65805903248921 40.0 37.0 41.0 33.0 41.0 52-53 37.511550746291405 40.0 37.0 41.0 33.0 41.0 54-55 37.30893720175024 40.0 36.0 41.0 32.5 41.0 56-57 37.122657834154076 40.0 36.0 41.0 32.0 41.0 58-59 36.88506616761446 39.0 35.0 41.0 32.0 41.0 60-61 36.62882483877361 39.0 35.0 41.0 31.0 41.0 62-63 36.369160784253936 39.0 35.0 41.0 31.0 41.0 64-65 36.06425043069933 38.0 35.0 41.0 31.0 41.0 66-67 35.76387194889543 37.0 35.0 40.0 31.0 41.0 68-69 35.43109382384778 37.0 35.0 39.5 31.0 41.0 70-71 35.17473815005108 36.0 35.0 39.0 30.5 41.0 72-73 35.00150173042033 36.0 35.0 39.0 31.0 41.0 74-75 34.69713451540608 35.5 35.0 38.5 31.0 40.5 76-77 34.37157536857191 35.0 35.0 37.0 31.0 39.0 78-79 34.051445701391955 35.0 35.0 37.0 30.0 39.0 80-81 33.78144867436082 35.0 35.0 36.0 30.0 39.0 82-83 33.54298798615663 35.0 34.0 36.0 30.0 37.0 84-85 33.36413913494229 35.0 34.0 36.0 30.0 37.0 86-87 33.15870889298837 35.0 34.0 35.0 30.0 36.5 88-89 33.00231929685475 35.0 34.0 35.0 30.0 36.0 90-91 32.8840580262536 35.0 34.0 35.0 29.5 36.0 92-93 32.76766438993155 35.0 34.0 35.0 29.0 36.0 94-95 32.66885891357046 35.0 34.0 35.0 29.0 36.0 96-97 32.6014639965849 35.0 34.0 35.0 29.0 35.0 98-99 32.53186603344971 35.0 34.0 35.0 29.0 35.0 100-101 32.494688676800166 35.0 34.0 35.0 29.0 35.0 102-103 31.704420194843806 34.5 32.5 35.0 26.0 35.0 104-105 32.338146620725404 35.0 34.0 35.0 29.0 35.0 106-107 32.399873839398694 35.0 34.0 35.0 29.0 35.0 108-109 32.395770761232484 35.0 34.0 35.0 29.0 35.0 110-111 32.354941988992394 35.0 34.0 35.0 29.0 35.0 112-113 32.29866902471376 35.0 34.0 35.0 29.0 35.0 114-115 32.2304927505298 35.0 34.0 35.0 29.0 35.0 116-117 32.151966352090994 35.0 34.0 35.0 29.0 35.0 118-119 32.07029737311521 35.0 34.0 35.0 28.5 35.0 120-121 31.958447043039442 35.0 34.0 35.0 27.0 35.0 122-123 31.804656888902443 35.0 34.0 35.0 27.0 35.0 124-125 30.05853699440472 34.0 30.5 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14737491436963523 1101 2 0.14545907041323147 1101 3 0.09631020565803539 1101 4 0.02821904357397642 1101 5 0.0454449434553581 1101 6 0.11844254389877307 1101 7 0.23121688360927806 1101 8 0.08413611099537377 1101 9 0.09441043686534556 1101 10-11 0.21190058466400075 1101 12-13 0.06634655923352284 1101 14-15 0.31500064306757025 1101 16-17 0.26179820181644686 1101 18-19 0.3088889112734705 1101 20-21 0.21858008393677153 1101 22-23 0.26076266056082886 1101 24-25 0.31045351760986506 1101 26-27 0.25259278384421435 1101 28-29 0.21634327921447039 1101 30-31 0.41005455283243464 1101 32-33 0.37459276802322705 1101 34-35 0.207789647013783 1101 36-37 0.29886027137678184 1101 38-39 0.508474807716027 1101 40-41 0.41305511012294716 1101 42-43 0.4908970795383354 1101 44-45 0.3781891336401699 1101 46-47 0.3322588263695536 1101 48-49 0.5007258907258105 1101 50-51 0.42500955032389953 1101 52-53 0.3978650297960229 1101 54-55 0.3306311430990405 1101 56-57 0.4003753856054004 1101 58-59 0.5433142485682083 1101 60-61 0.42093917847791573 1101 62-63 0.286753450984385 1101 64-65 0.22362678492551424 1101 66-67 0.46982361213766666 1101 68-69 0.0947120473410692 1101 70-71 0.19872487960271457 1101 72-73 0.05895985810609261 1101 74-75 -0.03314072878677621 1101 76-77 0.06601513867932596 1101 78-79 -0.1847266634519471 1101 80-81 -0.11233348125132636 1101 82-83 -0.1247602100201135 1101 84-85 -0.07714313423760899 1101 86-87 -0.1435080319788753 1101 88-89 -0.16460387054434733 1101 90-91 -0.19686710400426932 1101 92-93 -0.06787023638091227 1101 94-95 -0.08323049898736912 1101 96-97 -0.03831651445527484 1101 98-99 0.057000991127388545 1101 100-101 0.036810848903265025 1101 102-103 0.10608083825921355 1101 104-105 0.03914946681646825 1101 106-107 0.20088479519733937 1101 108-109 -0.034817510232457494 1101 110-111 -0.2256915368189496 1101 112-113 -0.29996066044602543 1101 114-115 -0.27670300051881824 1101 116-117 0.08071780289181163 1101 118-119 -0.015934581665383973 1101 120-121 -0.029320679024710472 1101 122-123 -0.04484491393445467 1101 124-125 -0.2762193703492919 1102 1 0.0011032921162410503 1102 2 -0.020301799151987154 1102 3 0.06188991580295777 1102 4 -0.018064266383944982 1102 5 -0.09977478538989715 1102 6 -0.12527531491375754 1102 7 -0.007913551173331257 1102 8 0.13982802310383136 1102 9 0.1598908015217262 1102 10-11 -0.013993524732917706 1102 12-13 0.12567626937845944 1102 14-15 0.05307918444204773 1102 16-17 0.04512276937138182 1102 18-19 0.08641345545299828 1102 20-21 0.002781229331809243 1102 22-23 0.06328428281422305 1102 24-25 0.11401242364071607 1102 26-27 0.03761206855669741 1102 28-29 -0.026644098064629418 1102 30-31 -0.17903497498430454 1102 32-33 -0.38139718055087 1102 34-35 -0.15260072231767907 1102 36-37 -0.3099522578424754 1102 38-39 -0.3989293531067801 1102 40-41 -0.3951934873525076 1102 42-43 -0.07163213972300042 1102 44-45 -0.1962469346160347 1102 46-47 -0.5474454747057109 1102 48-49 -0.45341447860565154 1102 50-51 -0.4682963169037748 1102 52-53 -0.1859650309800358 1102 54-55 -0.16026705699446353 1102 56-57 -0.20342604029455202 1102 58-59 -0.4157349566678832 1102 60-61 -0.8525384980064246 1102 62-63 -0.7298138701937305 1102 64-65 -0.6142939724377428 1102 66-67 -0.8177085290498027 1102 68-69 -0.6999934225046545 1102 70-71 -0.6993145079615601 1102 72-73 -0.6005773087943282 1102 74-75 -0.504169831171076 1102 76-77 -0.4952430427138452 1102 78-79 -0.5639780659764924 1102 80-81 -0.5509730324434727 1102 82-83 -0.6629790038490029 1102 84-85 -0.7832704133399844 1102 86-87 -0.5094354513337152 1102 88-89 -0.40231717115210586 1102 90-91 -0.30379499086728856 1102 92-93 -0.41930490211257165 1102 94-95 -0.3273135985666116 1102 96-97 -0.46371974026172325 1102 98-99 -0.5409711921358138 1102 100-101 -0.6183872576886067 1102 102-103 -0.7820093908197947 1102 104-105 -0.5444906687609077 1102 106-107 -0.4777763782481941 1102 108-109 -0.44602017502909064 1102 110-111 -0.41680011559407504 1102 112-113 -0.39970060902012605 1102 114-115 -0.452135445586606 1102 116-117 -0.5359985365191271 1102 118-119 -0.3982101777383136 1102 120-121 -0.5125049941252229 1102 122-123 -0.7388900284739606 1102 124-125 -0.8266518154170797 1103 1 0.03195188264225379 1103 2 -0.01624774509793525 1103 3 -0.05207404816100336 1103 4 -0.338290944675137 1103 5 -0.08026010942360529 1103 6 -0.03785886620698875 1103 7 0.11235671909693679 1103 8 0.08510555870395109 1103 9 0.06111137693584823 1103 10-11 -0.016521859519315285 1103 12-13 -0.024774181071997248 1103 14-15 0.06711580170447462 1103 16-17 -0.004557556115393879 1103 18-19 -0.05497712867952487 1103 20-21 -0.0784160970268033 1103 22-23 -0.09864538879287466 1103 24-25 -0.06262227557462552 1103 26-27 -0.2358840081651863 1103 28-29 -0.013239564498810807 1103 30-31 0.09499873614618792 1103 32-33 0.022932956037330143 1103 34-35 -0.0301363224339255 1103 36-37 -0.12273197304154593 1103 38-39 0.1313743376807821 1103 40-41 0.2276923016623087 1103 42-43 0.17365266120696532 1103 44-45 0.17320235338164736 1103 46-47 -0.04443036281963941 1103 48-49 -0.12284197061379132 1103 50-51 -0.2645982640121787 1103 52-53 -0.023330622377891075 1103 54-55 0.044666101970953775 1103 56-57 0.012383317450293418 1103 58-59 0.11119762107114894 1103 60-61 0.028731481650652313 1103 62-63 -0.046574986148399944 1103 64-65 -0.15773337958682987 1103 66-67 -0.2476925453241421 1103 68-69 -0.08249124290569654 1103 70-71 0.20335913342175616 1103 72-73 0.06389522825885052 1103 74-75 0.1280446413659817 1103 76-77 0.07305832316816208 1103 78-79 -0.01118817931563143 1103 80-81 0.20972879841964698 1103 82-83 -0.050053982053015034 1103 84-85 0.007749638970388162 1103 86-87 0.017133859913016636 1103 88-89 -0.13140028652554037 1103 90-91 -0.05737679848135713 1103 92-93 -0.2661590142892578 1103 94-95 -0.012012813910985187 1103 96-97 0.14918348554472516 1103 98-99 0.04693929900048488 1103 100-101 -0.20269414522130091 1103 102-103 -0.3498239796021245 1103 104-105 -0.2468155743116256 1103 106-107 -0.3362259568590673 1103 108-109 -0.3084220930761674 1103 110-111 -0.04676233587887424 1103 112-113 -0.09604614810760381 1103 114-115 -0.1966501215528993 1103 116-117 0.03082796740415006 1103 118-119 -0.17972717861015752 1103 120-121 -0.13438090229145416 1103 122-123 -0.011310836378640943 1103 124-125 0.024779454562576575 1104 1 -0.2072122151425333 1104 2 -0.20001002196365647 1104 3 -0.09350354131463234 1104 4 -0.18859562601430468 1104 5 -0.6264354624955146 1104 6 -0.04798145563992051 1104 7 -0.20751567318924913 1104 8 -0.042882110663029493 1104 9 -0.2834222813909122 1104 10-11 -0.10725954680872718 1104 12-13 -0.29695856704948653 1104 14-15 -0.1484695449153577 1104 16-17 -0.0922663524448879 1104 18-19 -0.048768798333256314 1104 20-21 -0.4040953571888224 1104 22-23 -0.38556201364053067 1104 24-25 -0.4240854628998889 1104 26-27 -0.3443935787437198 1104 28-29 -0.1822122507128725 1104 30-31 -0.4051123829304544 1104 32-33 -0.34361303292244827 1104 34-35 -0.11090196871681002 1104 36-37 -0.1559171476060328 1104 38-39 -0.028984827850806028 1104 40-41 0.06655888708062463 1104 42-43 -0.16085877330447573 1104 44-45 -0.3686357712151036 1104 46-47 -0.3850820224368121 1104 48-49 -0.04102606983236967 1104 50-51 -0.1365209824866227 1104 52-53 -0.11351629877046321 1104 54-55 -0.11202613403163042 1104 56-57 -0.6215259233554988 1104 58-59 -0.31338120178354245 1104 60-61 -0.42793566294751884 1104 62-63 -0.15619574749329956 1104 64-65 -0.013016912456564 1104 66-67 -0.36085119468968685 1104 68-69 -0.07450142284691452 1104 70-71 -0.23903100207182604 1104 72-73 -0.2733547538187153 1104 74-75 -0.2164997704198086 1104 76-77 -0.05871456377713713 1104 78-79 -0.09071717781015565 1104 80-81 -0.06463137076810455 1104 82-83 -0.07134941541741568 1104 84-85 0.0033514742273936804 1104 86-87 -0.002344238875430449 1104 88-89 0.029783310864949897 1104 90-91 -0.02113405706620597 1104 92-93 -0.11866815472943415 1104 94-95 0.09933566985129261 1104 96-97 0.07822857838291952 1104 98-99 0.1180017407525753 1104 100-101 0.12029675018338537 1104 102-103 -0.10103195149974908 1104 104-105 0.049409961569089944 1104 106-107 -0.09099690818173656 1104 108-109 0.04528426580260714 1104 110-111 -0.006415357600864979 1104 112-113 0.02568300810431623 1104 114-115 -0.06970553771176213 1104 116-117 -0.07065242545554895 1104 118-119 -0.08613265570528839 1104 120-121 -0.08349911865104787 1104 122-123 0.043574818507018165 1104 124-125 0.07434573171506287 1105 1 -0.08620510413175708 1105 2 -0.13732307574773017 1105 3 -0.07020228277860241 1105 4 -0.12985218676829646 1105 5 -0.2764792008554622 1105 6 0.0537455041207906 1105 7 -0.0744029128754562 1105 8 0.04273925623126473 1105 9 0.10429711586626667 1105 10-11 0.12928032564457226 1105 12-13 0.024169177179871326 1105 14-15 0.12385818238302448 1105 16-17 0.07640851634003099 1105 18-19 0.20776726464997353 1105 20-21 0.1426376695056888 1105 22-23 0.19713523225370722 1105 24-25 0.010846672096448629 1105 26-27 0.018694772743380383 1105 28-29 0.08261293693879423 1105 30-31 -0.01090983199642892 1105 32-33 0.07151691233404733 1105 34-35 -0.04787640293996276 1105 36-37 -0.038790052397501995 1105 38-39 0.06536657459854922 1105 40-41 0.01322277894811208 1105 42-43 0.26876479461697045 1105 44-45 0.3133475517715212 1105 46-47 -0.0573688332049187 1105 48-49 0.1180651599210023 1105 50-51 -0.3904680167436254 1105 52-53 -0.2427455159949119 1105 54-55 -0.11811423155405976 1105 56-57 -0.24118913798386643 1105 58-59 -0.6668788596649122 1105 60-61 -0.2943996281825889 1105 62-63 -0.38432656751700023 1105 64-65 -0.347950458367734 1105 66-67 -0.4754527523396703 1105 68-69 -0.5815136695877001 1105 70-71 -0.4519168872798005 1105 72-73 -0.3803902806543178 1105 74-75 -0.3840600164833532 1105 76-77 -0.32647445429014965 1105 78-79 -0.4325007152558342 1105 80-81 -0.43772663573790993 1105 82-83 -0.44373569568153926 1105 84-85 -0.4012354099082742 1105 86-87 -0.44869420219163914 1105 88-89 -0.5299195504703391 1105 90-91 -0.3339797312013104 1105 92-93 -0.30714521325418787 1105 94-95 -0.3547672704952305 1105 96-97 -0.2869867272212332 1105 98-99 -0.1328694991593835 1105 100-101 -0.27294285137426044 1105 102-103 -0.3493423809822289 1105 104-105 -0.4322022908153542 1105 106-107 -0.41593412247148365 1105 108-109 -0.3072504427023901 1105 110-111 -0.27804468205484056 1105 112-113 -0.12364821826285777 1105 114-115 -0.35388847692954783 1105 116-117 -0.2911889963681311 1105 118-119 -0.22580969729905576 1105 120-121 0.002623123010444317 1105 122-123 -0.1043384384487922 1105 124-125 0.3021802596229577 1106 1 0.13095615006757555 1106 2 0.04022451663748683 1106 3 0.07680219650471543 1106 4 0.01860805959228884 1106 5 0.10053647663613674 1106 6 5.0095386185944335E-5 1106 7 0.12892855408982484 1106 8 -0.11283185239135918 1106 9 0.07618961306671679 1106 10-11 -0.062041062933253954 1106 12-13 0.01427276060653071 1106 14-15 -0.13580027226422686 1106 16-17 -0.0969117524169647 1106 18-19 -0.04053900634666263 1106 20-21 0.07691818462326694 1106 22-23 0.04132616170500114 1106 24-25 0.0835370416373209 1106 26-27 0.10543889368402404 1106 28-29 -0.21043017616310777 1106 30-31 0.14709688692854428 1106 32-33 0.2767380769477086 1106 34-35 0.49674845708243964 1106 36-37 0.1964003171433788 1106 38-39 0.07383521961534711 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-0.4833066125273149 2216 102-103 -0.6304636918950592 2216 104-105 -0.7706331418791876 2216 106-107 -0.6968623997535701 2216 108-109 -0.7423911427710266 2216 110-111 -0.6086683951639671 2216 112-113 -0.8419049100953941 2216 114-115 -0.9306300692425182 2216 116-117 -1.156966278454611 2216 118-119 -0.9274440487060573 2216 120-121 -0.8011474672435028 2216 122-123 -0.8773308886890128 2216 124-125 -1.2708722455246075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 3.0 5 11.0 6 43.0 7 90.0 8 125.0 9 226.0 10 351.0 11 508.0 12 603.0 13 967.0 14 1478.0 15 1653.0 16 818.0 17 616.0 18 654.0 19 583.0 20 515.0 21 557.0 22 585.0 23 660.0 24 888.0 25 1118.0 26 1447.0 27 1722.0 28 2272.0 29 2820.0 30 3498.0 31 4513.0 32 5821.0 33 7914.0 34 11338.0 35 19218.0 36 41824.0 37 96880.0 38 49683.0 39 360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.902378211042404 17.521673821378737 11.743894349175365 23.832053618403496 2 16.59742338771154 19.254459521268487 38.532169538039334 25.61594755298064 3 20.506555877420336 22.915078518066778 29.14087513340448 27.4374904711084 4 12.731593623522322 14.953595446898923 35.57873134186023 36.73607958771853 5 12.832797807584265 38.37489851691766 34.21494974443415 14.577353931063925 6 34.16173930944566 35.03284269435898 15.877336444144547 14.92808155205081 7 29.696528487140007 33.080758030827404 18.937811590004728 18.284901892027868 8 28.279794483999325 34.58172615145371 17.99522800384199 19.143251360704973 9 27.2396365355003 15.084005427573905 18.821942034730373 38.85441600219542 10-11 26.705455016694362 25.595356070192555 25.80803768809745 21.891151225015626 12-13 26.801123629766277 22.870706346907347 27.022952844140203 23.30521717918617 14-15 22.768748761262977 24.209114055281976 24.92262657986614 28.099510603588907 16-17 22.912632830723727 28.543931332042504 25.450709701026053 23.092726136207713 18-19 22.985241877696634 25.704555503041576 28.41586498147612 22.89433763778567 20-21 23.041270906069432 25.171898583647145 29.088213321949656 22.698617188333763 22-23 22.9330243478526 25.173994907837965 29.053147535485053 22.83983320882438 24-25 22.605918192715748 25.457528858193214 29.258024740767 22.678528208324042 26-27 22.471642450946014 25.987368693875684 28.650462715921392 22.890526139256913 28-29 22.33017294773453 26.01114869693649 29.110295869264853 22.548382486064128 30-31 22.346506065428485 25.481660774354626 29.205347868761848 22.966485291455037 32-33 22.120222286594196 25.763252580384506 29.1139409370188 23.002584196002502 34-35 22.737113323474254 25.81947218368374 28.76042444847616 22.68299004436584 36-37 23.115346133689073 25.403973509933774 28.48978036114155 22.9908999952356 38-39 22.55339909438795 25.557812809684254 28.797205409278714 23.091582686649083 40-41 22.815292523540812 25.445612670969858 28.860531285792423 22.87856351969691 42-43 22.966756109832144 25.480820539403272 28.696010123340095 22.856413227424493 44-45 22.7865953132385 25.577459634999773 28.78131241519157 22.85463263657016 46-47 22.967693492197803 26.304685892011804 27.852547243884402 22.875073371905994 48-49 23.306741778597672 26.08894512966718 27.729032946593286 22.875280145141865 50-51 23.145091821099413 26.199696597778612 27.59035363892086 23.064857942201115 52-53 23.425660532695034 26.183279718254028 27.500533609794026 22.890526139256913 54-55 23.02536414867443 26.319330229437966 27.59315974134629 23.062145880541323 56-57 22.967137259685018 26.797502706163957 27.39476452562089 22.840595508530136 58-59 23.187458430131176 25.893097067192155 27.55055563072807 23.368888871948602 60-61 22.683604998408697 26.06707869044284 28.347502606117086 22.90181370503138 62-63 22.646371049968458 26.330494310201235 28.243547400378493 22.77958723945181 64-65 22.555267571276108 26.29230827870102 27.95052599481628 23.201898155206585 66-67 22.43727666873124 26.526275668207155 27.861070084329885 23.17537757873172 68-69 22.756257551560243 26.019865606049635 28.21148561344397 23.012391228946154 70-71 22.398339663778103 26.245585682077632 27.999878028560378 23.35619662558389 72-73 22.652235893213803 26.266218420771775 27.866143713122625 23.215401972891797 74-75 22.38893293287189 26.611120428107515 27.75094906313366 23.248997575886936 76-77 22.878078993758635 26.369431607032258 27.635046929343943 23.117442469865168 78-79 22.92739448778982 26.487919409367937 27.602215251388344 22.9824708514539 80-81 22.729239125256086 27.06522464147887 27.444661489351564 22.76087474391348 82-83 22.658154271284957 26.77002239268188 27.529658392491307 23.042164943541856 84-85 22.84002378375082 26.65933588449635 27.31967800460429 23.180962327148542 86-87 22.682581459127025 26.53575015580005 27.59233177882682 23.189336606246105 88-89 22.665307172601747 26.896446574742 27.147635337951805 23.290610914704455 90-91 22.690568260000344 27.022128540309165 27.269123529893825 23.018179669796666 92-93 22.739825235132123 26.43790320275202 27.46495649936631 23.357315062749546 94-95 22.716279495257314 26.330133427863 27.47668681139616 23.476900265483522 96-97 22.896996771500774 26.102244702707438 27.54897065381874 23.451787871973043 98-99 22.83715327938525 26.17409501012003 27.14740938666138 23.841342323833338 100-101 22.381107572560925 26.268684593203318 27.67406712826641 23.676140705969352 102-103 22.562152787703802 25.92588357489304 27.642243884775546 23.869719752627617 104-105 22.22086698658698 26.13881085696208 27.68441474784359 23.955907408607356 106-107 22.727012852373036 26.14020978487902 27.372276684301198 23.76050067844674 108-109 22.775881423542618 25.889523548145483 27.054018460389067 24.280576567922836 110-111 22.593198922835615 26.164117009172667 27.419284139269045 23.82339992872267 112-113 22.219426990125843 26.470975008719215 27.568357718556975 23.741240282597964 114-115 22.11602963821256 26.396151911085365 27.44278940708329 24.04502904361879 116-117 22.16386394474852 26.23073287493711 27.656995624399688 23.948407555914685 118-119 22.09360385118215 26.48907421453646 27.03850800995575 24.378813924325645 120-121 22.130936658490988 26.954397238945205 27.02795930074115 23.886706801822662 122-123 22.23003594311611 26.529488220339303 27.086358767947736 24.154117068596847 124-125 22.0047128444075 26.776467807002156 26.89848319619312 24.32033615239722 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 76.0 1 51.0 2 74.5 3 127.5 4 75.0 5 38.0 6 141.5 7 323.0 8 553.0 9 668.5 10 449.5 11 200.5 12 162.0 13 173.5 14 194.0 15 231.0 16 263.0 17 294.0 18 312.0 19 320.5 20 380.5 21 442.0 22 506.5 23 627.0 24 793.5 25 990.5 26 1202.5 27 1457.0 28 1788.5 29 2158.0 30 2551.0 31 3017.0 32 3769.0 33 4118.0 34 4145.0 35 4464.5 36 4972.0 37 5445.0 38 5847.5 39 6279.5 40 6552.5 41 7220.0 42 7967.0 43 8487.5 44 8926.5 45 9487.0 46 9692.5 47 9628.5 48 9955.0 49 10012.5 50 9773.0 51 9540.0 52 9532.5 53 9658.0 54 9712.5 55 9171.5 56 8107.0 57 7590.0 58 7359.0 59 6700.0 60 5666.0 61 4640.5 62 3865.5 63 3065.5 64 2461.0 65 2026.5 66 1531.0 67 1324.5 68 990.5 69 546.0 70 370.0 71 277.5 72 218.0 73 169.5 74 154.5 75 140.5 76 91.5 77 47.5 78 22.0 79 14.5 80 11.0 81 5.0 82 4.0 83 3.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025155890289826347 2 0.001524599411504627 3 0.001524599411504627 4 0.0811849186626214 5 0.001905749264380784 6 0.02020094220243631 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013340244850665486 26-27 0.0 28-29 5.717247793142351E-4 30-31 0.006288972572456587 32-33 0.0 34-35 0.0 36-37 5.717247793142351E-4 38-39 0.0 40-41 1.9057492643807837E-4 42-43 0.0 44-45 0.003049198823009254 46-47 7.622997057523135E-4 48-49 0.0 50-51 0.0022868991172569405 52-53 0.0 54-55 0.002096324190818862 56-57 0.0 58-59 0.0017151743379427056 60-61 1.9057492643807837E-4 62-63 0.003239773749447333 64-65 0.001524599411504627 66-67 5.717247793142351E-4 68-69 3.8114985287615675E-4 70-71 0.0028586238965711756 72-73 0.003049198823009254 74-75 0.0 76-77 5.717247793142351E-4 78-79 3.8114985287615675E-4 80-81 5.717247793142351E-4 82-83 5.717247793142351E-4 84-85 0.0 86-87 0.003239773749447333 88-89 0.004383223308075803 90-91 0.004002073455199646 92-93 0.004383223308075803 94-95 0.00476437316095196 96-97 0.004954948087390038 98-99 0.004954948087390038 100-101 0.00590782271958043 102-103 0.002477474043695019 104-105 0.0026680489701330972 106-107 0.0 108-109 0.0070512722782089 110-111 0.003239773749447333 112-113 0.00476437316095196 114-115 0.0 116-117 0.0 118-119 0.0011434495586284702 120-121 1.9057492643807837E-4 122-123 0.001905749264380784 124-125 0.03887728499336799 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 262364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.523101491294284 #Duplication Level Percentage of deduplicated Percentage of total 1 81.33509340355296 50.85322299674243 2 11.033531499878297 13.797012195485664 3 3.0962092030997477 5.807538067310549 4 1.4455809631254237 3.6152882108549527 5 0.7524322583358973 2.3522199226629534 6 0.4890096432368112 1.8344639732590058 7 0.3361854164207004 1.4713548437535138 8 0.2548443997259664 1.2746929814843653 9 0.18684118911748218 1.0513701566951805 >10 0.9818001909855388 11.177962090299607 >50 0.05796430406561951 2.3709296452615503 >100 0.028677076748253868 3.3597389196811696 >500 6.101505691117843E-4 0.19570183556221646 >1k 0.0012203011382235687 0.8385041609468844 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1153 0.4394657803662088 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1045 0.3983015962555838 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 513 0.1955298745254684 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 484 0.1844765287920599 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 383 0.14598039365156806 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 316 0.12044335350886555 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 312 0.11891875409736091 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 293 0.11167690689271395 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 290 0.11053345733408547 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 288 0.10977115762833316 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 283 0.10786540836395238 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 270 0.10291046027656234 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 3.811498528761568E-4 0.0 0.0 0.0 0.0 16-17 3.811498528761568E-4 0.0 0.0 0.0 0.0 18-19 3.811498528761568E-4 0.0 0.0 0.0 0.0 20-21 3.811498528761568E-4 0.0 0.0 0.0 0.0 22-23 3.811498528761568E-4 0.0 0.0 0.0 1.905749264380784E-4 24-25 3.811498528761568E-4 0.0 0.0 3.811498528761568E-4 3.811498528761568E-4 26-27 3.811498528761568E-4 0.0 0.0 3.811498528761568E-4 3.811498528761568E-4 28-29 3.811498528761568E-4 0.0 0.0 0.0017151743379427058 3.811498528761568E-4 30-31 3.811498528761568E-4 0.0 0.0 0.007813571983961215 3.811498528761568E-4 32-33 3.811498528761568E-4 0.0 0.0 0.013911969629979724 3.811498528761568E-4 34-35 5.717247793142352E-4 0.0 0.0 0.020582092055312466 3.811498528761568E-4 36-37 7.622997057523136E-4 0.0 0.0 0.03220716256803525 3.811498528761568E-4 38-39 7.622997057523136E-4 0.0 0.0 0.05488557881416658 3.811498528761568E-4 40-41 7.622997057523136E-4 0.0 0.0 0.07680169535454559 3.811498528761568E-4 42-43 7.622997057523136E-4 0.0 0.0 0.09757436233629614 3.811498528761568E-4 44-45 7.622997057523136E-4 0.0 0.0 0.12806635056638868 3.811498528761568E-4 46-47 7.622997057523136E-4 0.0 0.0 0.1658001860011282 3.811498528761568E-4 48-49 7.622997057523136E-4 0.0 0.0 0.19457699989327804 3.811498528761568E-4 50-51 7.622997057523136E-4 0.0 0.0 0.24107728194416916 3.811498528761568E-4 52-53 7.622997057523136E-4 0.0 0.0 0.29157963745025994 3.811498528761568E-4 54-55 7.622997057523136E-4 0.0 0.0 0.34246314280922685 3.811498528761568E-4 56-57 7.622997057523136E-4 0.0 0.0 0.40687746794529733 3.811498528761568E-4 58-59 7.622997057523136E-4 0.0 0.0 0.4678614444054825 3.811498528761568E-4 60-61 7.622997057523136E-4 0.0 0.0 0.5358966931438764 3.811498528761568E-4 62-63 7.622997057523136E-4 0.0 0.0 0.6005015932063851 3.811498528761568E-4 64-65 7.622997057523136E-4 0.0 0.0 0.6675839673125886 3.811498528761568E-4 66-67 7.622997057523136E-4 0.0 0.0 0.7556295833269808 3.811498528761568E-4 68-69 7.622997057523136E-4 0.0 0.0 0.8673064902196947 3.811498528761568E-4 70-71 7.622997057523136E-4 0.0 0.0 0.9974691649769023 3.811498528761568E-4 72-73 7.622997057523136E-4 0.0 0.0 1.1289658642191764 3.811498528761568E-4 74-75 7.622997057523136E-4 0.0 0.0 1.2463600189050328 3.811498528761568E-4 76-77 7.622997057523136E-4 0.0 0.0 1.390053513439344 3.811498528761568E-4 78-79 7.622997057523136E-4 0.0 0.0 1.548040127456511 3.811498528761568E-4 80-81 7.622997057523136E-4 0.0 0.0 1.7422359774969127 3.811498528761568E-4 82-83 7.622997057523136E-4 0.0 0.0 1.9147062859233737 3.811498528761568E-4 84-85 7.622997057523136E-4 0.0 0.0 2.094799591407358 3.811498528761568E-4 86-87 7.622997057523136E-4 0.0 0.0 2.2884237166684454 3.811498528761568E-4 88-89 7.622997057523136E-4 0.0 0.0 2.520543977070025 3.811498528761568E-4 90-91 7.622997057523136E-4 0.0 0.0 2.7820127761430684 3.811498528761568E-4 92-93 7.622997057523136E-4 0.0 0.0 3.0602521687426627 3.811498528761568E-4 94-95 7.622997057523136E-4 0.0 0.0 3.3314402890640484 3.811498528761568E-4 96-97 7.622997057523136E-4 0.0 0.0 3.597864036224482 3.811498528761568E-4 98-99 7.622997057523136E-4 0.0 0.0 3.9065954170541692 3.811498528761568E-4 100-101 9.528746321903921E-4 0.0 0.0 4.274595600006099 3.811498528761568E-4 102-103 0.0011434495586284705 0.0 0.0 4.674612370599625 3.811498528761568E-4 104-105 0.001905749264380784 0.0 0.0 5.048520376271135 3.811498528761568E-4 106-107 0.001905749264380784 0.0 0.0 5.416329984296626 3.811498528761568E-4 108-109 0.001905749264380784 0.0 0.0 5.8155844551844 3.811498528761568E-4 110-111 0.001905749264380784 0.0 0.0 6.302312817307253 3.811498528761568E-4 112-113 0.001905749264380784 0.0 0.0 6.807908097147474 3.811498528761568E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 395 0.0 82.98513 1 GTATCAA 905 0.0 64.53751 1 CAGCGTA 15 0.004225165 59.535904 118-119 ATCAACG 1100 0.0 52.53491 3 CAACGCA 1100 0.0 52.48483 5 TCAACGC 1105 0.0 52.29719 4 AACGCAG 1125 0.0 51.308712 6 TATCAAC 1160 0.0 49.817585 2 GCTAGAC 55 8.463216E-4 43.32776 3 TGGTATC 170 0.0 42.053413 2 ACGCAGA 1395 0.0 41.796604 7 CGCAGAG 1415 0.0 41.20584 8 GTATAGA 90 5.082218E-6 39.732277 1 CTATAGT 90 5.0955696E-6 39.71711 4 GTGGTAT 250 0.0 38.142986 1 GCAGAGT 1535 0.0 37.984535 9 GTAAGGT 65 0.0019241297 36.66195 2 CAGTCCG 65 0.0019367863 36.613037 9 ACATGGG 1140 0.0 33.445988 3 GTACATG 1125 0.0 33.375114 1 >>END_MODULE