FastQCFastQC Report
Fri 27 May 2016
SRR940135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266655
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46681.7505765877257131No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13200.4950216571975024No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.4657703774540136No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6700.2512609926684293No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6620.24826086141268683No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6040.22650990980855415No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT5110.19163338396054827No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA4630.17363259642609363No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4420.16575725187976975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.15675685811254245No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3910.1466314151244117No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC3850.14438131668260487No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG3670.13763102135718439No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG3570.13388085728750632No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT3470.13013069321782827No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA3340.1252554799272468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.1237554142993756No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC3120.11700511897395512No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.1162550861600195No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.1155050533460839No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG2970.11137987286943803No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT2920.109504790834599No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA2910.1091297744276312No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC2880.10800472520672778No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT2840.10650465957885656No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA2820.10575462676492095No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT2810.10537961035795315No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA2770.10387954473008193No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA2770.10387954473008193No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT2680.10050439706737169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4300.073.363231
GACTGCC250.002055277371.398547
GTATCAA9550.064.8187941
TAAGACT300.00422704659.5211074
GATAGTC401.7696813E-459.4987837
GTGGTAC1150.056.9332351
GAACAAA5950.056.0198631
GTATGGT752.2972017E-855.5530321
CGTCACT453.160965E-452.907651
GTCCATT350.007790357350.9989556
TGGGAGT1051.10958354E-1050.998956
GGATATG856.1783794E-848.9989979
TCCCGCT1350.048.480498
CTCCCGC1400.046.749047
CATATAG1301.8189894E-1145.785473
ATCAACG11500.045.5465853
TCAACGC11650.045.4710584
GTACATG21250.045.0959931
GAAAACA5700.043.857661
CGCAGAA951.4776379E-743.857662
Produced by FastQC (version 0.11.5)