##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940135_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 266655 Sequences flagged as poor quality 0 Sequence length 125 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.046982055464927 31.0 30.0 34.0 15.0 34.0 2 29.26464157806904 33.0 30.0 34.0 16.0 34.0 3 29.53054696142956 33.0 30.0 34.0 18.0 34.0 4 32.090907727213065 37.0 33.0 37.0 17.0 37.0 5 32.93151450375954 37.0 33.0 37.0 23.0 37.0 6 32.29656297463014 37.0 35.0 37.0 15.0 37.0 7 32.4081378560312 37.0 33.0 37.0 15.0 37.0 8 32.05574618889577 37.0 33.0 37.0 13.0 37.0 9 33.10436331589507 39.0 34.0 39.0 10.0 39.0 10-11 33.49862556486846 39.0 34.0 39.0 11.5 39.0 12-13 33.40355515553806 39.0 34.0 39.0 11.5 39.0 14-15 34.60666966679793 40.0 34.0 41.0 12.5 41.0 16-17 34.80388516997618 40.0 34.0 41.0 13.0 41.0 18-19 34.82891001481315 40.0 34.0 41.0 14.5 41.0 20-21 34.88839511728638 40.0 34.0 41.0 17.5 41.0 22-23 34.968072978192794 40.0 34.0 41.0 20.0 41.0 24-25 35.07705087097561 40.0 34.0 41.0 23.5 41.0 26-27 35.06131705762127 40.0 34.0 41.0 25.0 41.0 28-29 34.93800791284619 40.0 33.5 41.0 25.0 41.0 30-31 34.7674598263674 40.0 33.0 41.0 24.0 41.0 32-33 34.632947066434156 40.0 33.0 41.0 23.0 41.0 34-35 34.462578987830724 40.0 33.0 41.0 19.5 41.0 36-37 34.234563387148185 40.0 33.0 41.0 13.5 41.0 38-39 34.01149800303763 40.0 33.0 41.0 7.5 41.0 40-41 33.83629408786635 40.0 33.0 41.0 2.0 41.0 42-43 33.683846168269866 40.0 33.0 41.0 2.0 41.0 44-45 33.70262886501284 40.0 33.0 41.0 2.0 41.0 46-47 33.5933153325458 40.0 33.0 41.0 2.0 41.0 48-49 33.45116348840262 40.0 33.0 41.0 2.0 41.0 50-51 33.312366166019764 40.0 33.0 41.0 2.0 41.0 52-53 33.15281543567531 40.0 32.0 41.0 2.0 41.0 54-55 33.00920290262699 39.5 32.0 41.0 2.0 41.0 56-57 32.835388423243515 39.0 32.0 41.0 2.0 41.0 58-59 32.60369391160863 39.0 31.0 41.0 2.0 41.0 60-61 32.37666460407643 39.0 31.0 41.0 2.0 41.0 62-63 32.101811329245656 38.0 31.0 41.0 2.0 41.0 64-65 31.810462957754403 37.5 31.0 40.5 2.0 41.0 66-67 31.502212596801108 37.0 31.0 40.0 2.0 41.0 68-69 31.188470120567775 37.0 30.5 40.0 2.0 41.0 70-71 31.03254204871463 36.0 31.0 39.5 2.0 41.0 72-73 30.75934447132062 36.0 31.0 39.0 2.0 41.0 74-75 30.405837130374454 35.5 30.0 39.0 2.0 41.0 76-77 30.04687892595301 35.0 30.0 37.5 2.0 40.0 78-79 29.699906245898255 35.0 30.0 37.0 2.0 39.0 80-81 29.353895482927378 35.0 30.0 37.0 2.0 39.0 82-83 29.025375485177477 35.0 30.0 36.0 2.0 38.5 84-85 28.708182858000036 35.0 29.0 36.0 2.0 37.0 86-87 28.473115073784477 35.0 29.0 36.0 2.0 37.0 88-89 28.25580619152088 35.0 29.0 35.0 2.0 36.5 90-91 28.077097748026475 35.0 29.0 35.0 2.0 36.0 92-93 27.908347865219103 35.0 28.0 35.0 2.0 36.0 94-95 27.773842605613993 35.0 28.0 35.0 2.0 36.0 96-97 27.621762202096342 35.0 28.0 35.0 2.0 36.0 98-99 27.5364478445932 35.0 28.0 35.0 2.0 35.0 100-101 27.467604207684087 35.0 27.5 35.0 2.0 35.0 102-103 26.79387785715625 34.0 25.5 35.0 2.0 35.0 104-105 27.271855018657067 35.0 27.0 35.0 2.0 35.0 106-107 27.292122780371642 35.0 27.0 35.0 2.0 35.0 108-109 27.25608557874407 35.0 27.0 35.0 2.0 35.0 110-111 27.158528810635467 35.0 26.0 35.0 2.0 35.0 112-113 27.069895557930657 35.0 25.5 35.0 2.0 35.0 114-115 26.94693517841406 35.0 25.0 35.0 2.0 35.0 116-117 26.85704187058184 35.0 25.0 35.0 2.0 35.0 118-119 26.718711443625658 35.0 25.0 35.0 2.0 35.0 120-121 26.552014775646434 35.0 25.0 35.0 2.0 35.0 122-123 26.33674973280081 35.0 24.5 35.0 2.0 35.0 124-125 24.56438469183027 32.0 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34486693296605964 1101 2 0.29805196987211247 1101 3 0.4745412422972741 1101 4 0.20400733806057048 1101 5 0.5050040802161178 1101 6 0.36458810768084504 1101 7 0.40930666485519396 1101 8 0.5871521072732975 1101 9 0.4464111792582912 1101 10-11 0.46773357939995464 1101 12-13 0.593785274326244 1101 14-15 0.4626332761276828 1101 16-17 0.4772161624915441 1101 18-19 0.42881817496847674 1101 20-21 0.4292989986783624 1101 22-23 0.33162626513528437 1101 24-25 0.44936776122702327 1101 26-27 0.5842980949955532 1101 28-29 0.7937100524097929 1101 30-31 0.9254710218646025 1101 32-33 1.0865681179169968 1101 34-35 0.7547402304632769 1101 36-37 0.8166541177067188 1101 38-39 0.6953593839393335 1101 40-41 0.6708421415275225 1101 42-43 0.5806475526990411 1101 44-45 0.5249748867987449 1101 46-47 0.5082963745115023 1101 48-49 0.5180780358500954 1101 50-51 0.7047275399784922 1101 52-53 0.6958915970853425 1101 54-55 0.7596623191116123 1101 56-57 0.706375132492397 1101 58-59 0.6840912019734873 1101 60-61 0.6697587507217548 1101 62-63 0.8967334867758296 1101 64-65 0.7869461758531955 1101 66-67 0.7737454069839664 1101 68-69 0.8165425709183971 1101 70-71 0.9175949320126691 1101 72-73 1.0981471342877782 1101 74-75 1.181859131963126 1101 76-77 0.872487572181452 1101 78-79 0.9396229907985862 1101 80-81 0.8741322616557561 1101 82-83 0.9169071357882856 1101 84-85 0.755783108634045 1101 86-87 0.7785482954164991 1101 88-89 0.8199642962360727 1101 90-91 0.688271418372544 1101 92-93 0.7290438012413176 1101 94-95 0.8969648037958287 1101 96-97 0.8558184784780423 1101 98-99 0.9305541985538781 1101 100-101 0.631936204406216 1101 102-103 0.45784009928035374 1101 104-105 0.33862235329261736 1101 106-107 0.47683168973766143 1101 108-109 0.22603431596609624 1101 110-111 -0.05442022990234818 1101 112-113 -0.09485082735153227 1101 114-115 0.007221095630544738 1101 116-117 -0.09436690108923784 1101 118-119 0.16507782567181195 1101 120-121 0.26176617279579517 1101 122-123 0.27528364476559375 1101 124-125 0.16204312916745778 1102 1 0.7341398631285152 1102 2 0.7364966751747808 1102 3 0.7942737176968926 1102 4 0.8193646413662208 1102 5 0.543466176067021 1102 6 0.6113817253496379 1102 7 0.5630898337029322 1102 8 0.757021533240291 1102 9 1.1948351608243115 1102 10-11 1.1396681329700868 1102 12-13 0.8539370068596099 1102 14-15 1.1561900780058565 1102 16-17 1.1225468002898964 1102 18-19 1.2161317700295555 1102 20-21 1.1171386578236167 1102 22-23 0.800268932845384 1102 24-25 0.9296548501941686 1102 26-27 0.9464453929391254 1102 28-29 0.8480047932181662 1102 30-31 0.911044112889634 1102 32-33 0.8286264342220804 1102 34-35 0.8791333727904274 1102 36-37 0.7603558408202034 1102 38-39 0.6876350820973798 1102 40-41 0.8612396604759809 1102 42-43 0.7063232479586645 1102 44-45 0.7821919643171071 1102 46-47 0.9265064523342019 1102 48-49 0.8576139980546387 1102 50-51 0.7575150999396456 1102 52-53 0.7842573203147722 1102 54-55 0.935868426092469 1102 56-57 0.7895490524668887 1102 58-59 0.6425459068413915 1102 60-61 0.9750389559302306 1102 62-63 1.015355140906813 1102 64-65 0.9504245753430709 1102 66-67 0.7413808721086674 1102 68-69 0.8207525328041179 1102 70-71 1.0185869178683546 1102 72-73 0.8689650697398612 1102 74-75 1.054219753965068 1102 76-77 1.1137488216923437 1102 78-79 1.0050549285404209 1102 80-81 1.0763589764362713 1102 82-83 1.1190541438893788 1102 84-85 0.9923452870900533 1102 86-87 1.080698201942301 1102 88-89 1.1712243863770766 1102 90-91 1.240185827890972 1102 92-93 1.271784696564712 1102 94-95 1.295012714756222 1102 96-97 1.3473116019654299 1102 98-99 1.350144651142898 1102 100-101 0.9560960235445108 1102 102-103 0.9417900724699244 1102 104-105 1.1050056989442538 1102 106-107 1.0621323288403097 1102 108-109 0.8060525760417434 1102 110-111 1.0722771600662035 1102 112-113 1.2782017935490089 1102 114-115 1.2455187058793733 1102 116-117 1.4656232442667623 1102 118-119 1.3316358014119984 1102 120-121 0.9824884602325739 1102 122-123 1.1955189968517317 1102 124-125 1.0964554010976642 1103 1 0.27518052181623176 1103 2 0.33392756672331814 1103 3 0.4196309312894009 1103 4 0.6607759533657713 1103 5 0.1962545641495268 1103 6 0.1935337780899289 1103 7 -0.03004034766254904 1103 8 0.14999892621768396 1103 9 0.1883133601848641 1103 10-11 0.34245935046718756 1103 12-13 0.041230105917421156 1103 14-15 0.004703220636649519 1103 16-17 0.14001136187211216 1103 18-19 0.23363169929419314 1103 20-21 0.5863192593571611 1103 22-23 0.09588616963908692 1103 24-25 0.4171700582976072 1103 26-27 0.37820698646541473 1103 28-29 0.4589249188441755 1103 30-31 0.5312588654573247 1103 32-33 0.4380328230402384 1103 34-35 0.3336859008723607 1103 36-37 0.5190350700876678 1103 38-39 0.57234351092346 1103 40-41 0.5014654477605873 1103 42-43 0.4928297697593109 1103 44-45 0.36401863437907167 1103 46-47 0.3586641639036756 1103 48-49 0.6992304426122544 1103 50-51 0.5923904642267814 1103 52-53 0.530515419514046 1103 54-55 0.48485266625317536 1103 56-57 0.42408064288083125 1103 58-59 0.3474025969921968 1103 60-61 0.350749846347 1103 62-63 0.2598448559847597 1103 64-65 0.43470717727273467 1103 66-67 0.6243067697404463 1103 68-69 0.6952043337265046 1103 70-71 0.4942822465048593 1103 72-73 0.5926302934050867 1103 74-75 0.6741161973909193 1103 76-77 0.5294110461780974 1103 78-79 0.9132970132530929 1103 80-81 0.8078333843812686 1103 82-83 0.7531118073588097 1103 84-85 0.6760308825403527 1103 86-87 0.6944406686259477 1103 88-89 0.6513618959263532 1103 90-91 0.8140618429434561 1103 92-93 0.7384966338648837 1103 94-95 0.8569273796120633 1103 96-97 0.5438427396730354 1103 98-99 0.48946502575739004 1103 100-101 0.3872917341519866 1103 102-103 0.33004941661162235 1103 104-105 0.6741495180880754 1103 106-107 0.5522930395892551 1103 108-109 0.5402361484606111 1103 110-111 0.5534969206977287 1103 112-113 0.6483741048977905 1103 114-115 0.5829405433012127 1103 116-117 0.34368962233314093 1103 118-119 0.6080059454292019 1103 120-121 0.5141213153948421 1103 122-123 0.33058110250207307 1103 124-125 0.038091651557444095 1104 1 0.10070200440577892 1104 2 0.41099341342267337 1104 3 -0.12517640409384967 1104 4 -0.04161192441478079 1104 5 -0.14892120249221108 1104 6 -0.06111316503869091 1104 7 0.15103115016287916 1104 8 -0.18514886180969015 1104 9 -0.3835168844202528 1104 10-11 0.11168425974445029 1104 12-13 0.13297779975935242 1104 14-15 -0.07554830987665895 1104 16-17 -0.2669735961599713 1104 18-19 -0.41939193016922616 1104 20-21 -0.07736256008382014 1104 22-23 0.466464301997334 1104 24-25 0.36175301073397037 1104 26-27 0.28129294452519815 1104 28-29 0.7258990732528829 1104 30-31 0.7130921572382576 1104 32-33 0.6655668267522543 1104 34-35 0.6504467529735294 1104 36-37 0.9123492893181151 1104 38-39 0.9332884124442771 1104 40-41 0.9699064018036339 1104 42-43 0.745849243982974 1104 44-45 0.6784248506177519 1104 46-47 0.4637086249690725 1104 48-49 0.5179858097969472 1104 50-51 0.24355116119899378 1104 52-53 0.4797314642798298 1104 54-55 0.3390937809654986 1104 56-57 0.2835435088882008 1104 58-59 0.29404239310843394 1104 60-61 0.2513149589539978 1104 62-63 0.17198923671553246 1104 64-65 0.033193284921146926 1104 66-67 0.2819357899349164 1104 68-69 0.32359431682852957 1104 70-71 0.19430856302332344 1104 72-73 -0.13472364991744712 1104 74-75 0.015323710064404139 1104 76-77 -0.02829138325207836 1104 78-79 0.14675915901890235 1104 80-81 0.08550728116511053 1104 82-83 -0.026632783904105395 1104 84-85 0.0011576173529519451 1104 86-87 -0.01749733519687524 1104 88-89 0.029821133024334756 1104 90-91 0.1219928105602861 1104 92-93 0.11428266671897447 1104 94-95 0.14005849127720538 1104 96-97 0.1119124922977619 1104 98-99 0.15728130875691804 1104 100-101 0.03589695867344034 1104 102-103 0.12379024045715781 1104 104-105 0.17901807400374992 1104 106-107 0.25601059409214955 1104 108-109 0.17134781360368834 1104 110-111 0.3505550109495381 1104 112-113 0.519956420197385 1104 114-115 0.42376715899693806 1104 116-117 0.522738903476661 1104 118-119 0.5605254058020677 1104 120-121 0.5791231159987404 1104 122-123 0.314908923217331 1104 124-125 -0.05308123411806065 1105 1 0.6723779424511527 1105 2 0.6494450931783469 1105 3 0.5571970688555403 1105 4 0.7702336253234421 1105 5 0.7007188498638115 1105 6 0.8070423759735235 1105 7 0.6164543613321598 1105 8 0.6017185029372598 1105 9 1.122795833729839 1105 10-11 0.8789590197793586 1105 12-13 0.7718848678221804 1105 14-15 0.8680258066154849 1105 16-17 0.9396145364752897 1105 18-19 0.9597188355375792 1105 20-21 1.0159034194100656 1105 22-23 0.9248998931840617 1105 24-25 1.0271857659695698 1105 26-27 0.924999314507744 1105 28-29 0.9666754148759225 1105 30-31 0.6723798972033634 1105 32-33 0.6883635108709214 1105 34-35 0.6728889432004053 1105 36-37 0.40866800659560454 1105 38-39 0.5282586894948835 1105 40-41 0.6920036422050373 1105 42-43 0.6392624747857596 1105 44-45 0.5825206185060523 1105 46-47 0.575306028983043 1105 48-49 0.5257846754164959 1105 50-51 0.6962057750733379 1105 52-53 0.5960122779796464 1105 54-55 0.5844103712796311 1105 56-57 0.6095551799178693 1105 58-59 0.715044792759393 1105 60-61 0.8390931003152531 1105 62-63 0.8553102991064492 1105 64-65 0.9794533743626843 1105 66-67 0.9981906983118769 1105 68-69 0.7936873033032263 1105 70-71 0.9691913073514193 1105 72-73 1.0509016227701622 1105 74-75 0.9013607410417137 1105 76-77 0.9745582022627524 1105 78-79 0.9380986005546816 1105 80-81 0.829596777238411 1105 82-83 0.6651075084170088 1105 84-85 0.8727694738101945 1105 86-87 0.8723300535465803 1105 88-89 0.6822398721168277 1105 90-91 0.9115734170175323 1105 92-93 1.1312711047643553 1105 94-95 1.10428187696656 1105 96-97 1.1864311986677478 1105 98-99 1.0015971355457474 1105 100-101 1.077788592617594 1105 102-103 0.9369855938602996 1105 104-105 0.8982111913684996 1105 106-107 1.0216424112823752 1105 108-109 1.0535876696246333 1105 110-111 0.9511903730786848 1105 112-113 0.9079227160089012 1105 114-115 0.9313615089096778 1105 116-117 1.149927221539489 1105 118-119 1.2272602443710028 1105 120-121 1.1996578564001368 1105 122-123 1.1984713141422887 1105 124-125 1.2881123195468618 1106 1 -0.35198817511990654 1106 2 -0.3657155980361253 1106 3 -0.29422411977495244 1106 4 -0.5548632739013648 1106 5 -0.7725531139604769 1106 6 0.0743631124076316 1106 7 -0.11561928207868988 1106 8 -0.2823254247113205 1106 9 -0.3575215112314041 1106 10-11 -0.4423628471457093 1106 12-13 -0.27890647584706585 1106 14-15 -0.7368373232294729 1106 16-17 -0.5342226728270418 1106 18-19 -0.542915612136845 1106 20-21 -0.4500102595305009 1106 22-23 -0.2748841022939672 1106 24-25 -0.28019674372035297 1106 26-27 -0.16842768807622832 1106 28-29 -0.18199423951683968 1106 30-31 -9.485911687363568E-4 1106 32-33 -0.03305121781124143 1106 34-35 0.07950231529343199 1106 36-37 0.13965153372738826 1106 38-39 0.1307599007351925 1106 40-41 -0.09414178738153112 1106 42-43 0.056383356568709075 1106 44-45 -0.11890864505983245 1106 46-47 -0.08891796972497445 1106 48-49 -0.13321080261968632 1106 50-51 -0.019889589913745453 1106 52-53 -0.2124401606508428 1106 54-55 -0.2216195867307107 1106 56-57 -0.12286164824750756 1106 58-59 -0.25527981964370383 1106 60-61 -0.10329223560205492 1106 62-63 -0.2763616162682254 1106 64-65 -0.26548969734274763 1106 66-67 -0.2856230936777884 1106 68-69 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120-121 -0.9122976360241069 2216 122-123 -0.8877029282819571 2216 124-125 -1.1155098395440461 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9724.0 3 2317.0 4 2917.0 5 2733.0 6 3882.0 7 3329.0 8 2734.0 9 2231.0 10 2237.0 11 2335.0 12 2029.0 13 1839.0 14 1762.0 15 1712.0 16 1519.0 17 1294.0 18 1168.0 19 1052.0 20 906.0 21 890.0 22 963.0 23 1650.0 24 3339.0 25 2424.0 26 4120.0 27 2954.0 28 2496.0 29 2904.0 30 3171.0 31 3574.0 32 4809.0 33 6034.0 34 8513.0 35 14068.0 36 28069.0 37 77273.0 38 51586.0 39 98.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49834021627826 24.155198217561075 13.916676981706747 19.42978458445392 2 13.016033755274261 28.433942803563056 38.036568213783404 20.51345522737928 3 15.033098921706516 38.28898265353962 27.154992967651197 19.522925457102673 4 9.591800751047602 24.48632770735404 37.70018869977228 28.221682841826073 5 9.603178175925093 47.42804837789982 31.406995588367696 11.561777857807389 6 23.176815682273418 45.42000195053152 18.145569667734456 13.257612699460603 7 23.21589306609859 41.765804554592094 21.78208167061718 13.236220708692132 8 22.826637264718787 41.61313186998639 19.734932439274104 15.825298426020723 9 24.95564998556046 24.77187402720634 18.700516447948274 31.571959539284922 10-11 19.57968003480165 36.94769251539448 25.84041491970869 17.632212530095178 12-13 21.27093838073397 35.36905741893263 25.373013982984897 17.986990217348502 14-15 20.20733356212037 35.63339315551705 23.65601681772237 20.503256464640213 16-17 18.226855003084562 38.23375542142242 24.807003924612932 18.732385650880083 18-19 18.30601092896175 36.75416586968507 26.79920939425648 18.1406138070967 20-21 20.945342854142602 35.56727321822355 25.81213153118015 17.675252396453697 22-23 20.90443046044687 35.650880916843555 25.590653056020162 17.85403556668942 24-25 19.420343121168766 36.02340979708634 26.34989808374947 18.206348997995427 26-27 18.574597466009386 36.42841204629961 26.34157683444307 18.65541365324793 28-29 19.056887883782725 34.73598148804468 27.29050755986101 18.91662306831159 30-31 19.066870178622995 35.98805179804314 26.688442946023077 18.256635077310793 32-33 18.620323683337116 36.58510516614507 26.291067487469505 18.50350366304831 34-35 18.96667785467712 35.45786776521679 27.10811180760122 18.467342572504872 36-37 18.89797739998575 35.978502608415184 25.810746445542065 19.312773546057 38-39 18.372857456267823 36.5739551436988 26.422127758516268 18.631059641517112 40-41 18.119863925676714 36.15214969675829 27.256684632378036 18.471301745186953 42-43 18.165094949941963 36.39132089836315 26.26382713803792 19.179757013656964 44-45 17.9197644874884 36.62350812386908 26.3657756818331 19.090951706809424 46-47 17.93528811677693 36.19668601947282 26.55028729165729 19.317738572092953 48-49 17.776002640204617 36.858043998409876 26.06620763109141 19.2997457302941 50-51 18.122252726645364 36.39190182576474 26.036837841487014 19.449007606102885 52-53 18.082638739135735 36.44299014598198 26.288194867659882 19.186176247222406 54-55 18.32456707825875 36.443244358188245 26.119502339230632 19.112686224322374 56-57 18.18137493905412 36.60353298578555 25.500693845403745 19.71439822975659 58-59 17.643892751117253 35.8147655688853 26.60049097288772 19.940850707109732 60-61 18.34415280012301 36.869255088149586 25.141853147771037 19.644738963956364 62-63 17.882840056931652 36.85349475219916 26.0237290233296 19.239936167539582 64-65 17.992874554659664 35.727732983311455 26.289518094880933 19.989874367147948 66-67 17.85514051111657 36.550516310615166 25.404132360027976 20.19021081824029 68-69 17.94876122008524 36.22135092920213 25.90402901143244 19.92585883928019 70-71 18.197654903681535 35.338085031774874 26.44993371249412 20.014326352049476 72-73 17.768068928661833 36.06922867777351 25.633736792267086 20.528965601297568 74-75 17.54701130371515 36.332968294091614 25.922036618936534 20.197983783256703 76-77 17.75147818031456 35.31918005764575 27.156511775747905 19.772829986291786 78-79 17.577128113391876 35.14769332890806 26.958134336894172 20.317044220805887 80-81 18.17089109616398 36.17939308477424 26.01263484765239 19.63708097140939 82-83 18.782486405400338 34.5379711231952 26.572848303018937 20.106694168385523 84-85 18.43359103259086 34.468385120071105 26.44526906540111 20.652754781936924 86-87 18.618156316683603 35.074873148797494 26.50055690771833 19.806413626800577 88-89 18.728564544044765 33.93013518049381 27.193799961371646 20.147500314089786 90-91 18.88767418180727 33.9223933438219 26.93149364591518 20.25843882845565 92-93 19.217930108434324 34.695044877781655 26.149887664892557 19.937137348891465 94-95 18.37992912189908 33.5643434400255 27.014869400536274 21.04085803753914 96-97 18.91206720357019 33.96049165111897 26.732170146539037 20.395270998771807 98-99 18.192676876112184 34.710489652107555 26.7753240689522 20.321509402828067 100-101 17.430575087660085 35.06103392023401 27.145562618364554 20.36282837374135 102-103 18.33717725150475 35.55024282312351 26.375466426656168 19.73711349871557 104-105 17.122818861166586 37.136133067049215 26.224672536168697 19.516375535615502 106-107 17.205565243479402 36.51872269411787 26.586975680186008 19.688736382216725 108-109 17.349571543755037 37.266692917815156 25.26466782921753 20.11906770921228 110-111 16.944023071052616 38.43494109418742 25.048705347181468 19.572330487578498 112-113 16.672042244541288 38.4238180604415 25.95654032808527 18.947599366931943 114-115 16.802521709617114 38.57739424589669 25.351919878339885 19.268164166146313 116-117 16.57259969848568 38.82109400196509 25.44514614444186 19.161160155107368 118-119 16.473118602178562 38.06299422262309 25.55134477307716 19.912542402121183 120-121 16.52143911224618 37.95642610833282 25.47904466708294 20.04309011233806 122-123 16.68563628215537 38.13042720074103 25.51972683753696 19.66420967956663 124-125 16.714957532812143 38.117819704925296 25.37372251267635 19.793500249586213 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3542.0 1 3311.0 2 3448.5 3 3063.0 4 1751.0 5 990.5 6 717.5 7 650.5 8 674.5 9 674.5 10 550.5 11 418.0 12 383.5 13 380.5 14 381.0 15 390.5 16 389.0 17 389.0 18 427.0 19 470.5 20 534.5 21 596.5 22 687.0 23 796.0 24 873.0 25 1021.5 26 1298.5 27 1520.0 28 1718.5 29 2360.5 30 3690.5 31 4641.0 32 6036.0 33 8167.0 34 9873.5 35 11219.0 36 13897.5 37 16126.0 38 15952.0 39 15673.0 40 14145.5 41 11998.0 42 9993.0 43 9221.5 44 9602.0 45 9089.5 46 7531.0 47 6184.5 48 5735.5 49 5040.5 50 4432.0 51 3633.0 52 2973.0 53 3024.5 54 3136.5 55 3023.5 56 2837.5 57 2561.5 58 2290.5 59 2038.5 60 1896.0 61 1905.0 62 1696.0 63 1164.5 64 737.0 65 622.5 66 612.5 67 497.5 68 295.0 69 184.5 70 150.5 71 108.5 72 69.0 73 74.0 74 67.0 75 35.0 76 16.0 77 17.5 78 19.0 79 10.5 80 6.5 81 6.0 82 4.0 83 0.5 84 1.5 85 2.5 86 1.5 87 1.5 88 1.5 89 2.5 90 3.0 91 3.5 92 2.5 93 2.0 94 3.0 95 2.0 96 2.5 97 3.0 98 3.0 99 4.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020250885976261464 2 0.011250492209034145 3 0.011250492209034145 4 0.03525154225497366 5 0.03262642740619902 6 0.022876000825036095 7 0.008625377360259512 8 0.002250098441806829 9 0.009750426581162925 10-11 3.750164069678048E-4 12-13 0.0035626558661941464 14-15 0.011813016819485852 16-17 0.0016875738313551216 18-19 0.009000393767227315 20-21 0.004125180476645853 22-23 3.750164069678048E-4 24-25 0.005437737901033171 26-27 1.875082034839024E-4 28-29 0.00618777071496878 30-31 0.0015000656278712193 32-33 0.0020625902383229265 34-35 1.875082034839024E-4 36-37 0.0067502953254204865 38-39 0.001312557424387317 40-41 0.013500590650840973 42-43 0.005437737901033171 44-45 9.375410174195122E-4 46-47 0.010125442988130732 48-49 0.0033751476627102432 50-51 0.010125442988130732 52-53 0.00468770508709756 54-55 0.0028126230522585362 56-57 0.00937541017419512 58-59 0.015563180889163902 60-61 0.002250098441806829 62-63 0.007312819935872195 64-65 0.0018750820348390244 66-67 0.001312557424387317 68-69 5.625246104517072E-4 70-71 0.005062721494065366 72-73 9.375410174195122E-4 74-75 0.006375278918452682 76-77 0.009937934784646829 78-79 0.0016875738313551216 80-81 0.004312688680129756 82-83 0.0018750820348390244 84-85 1.875082034839024E-4 86-87 0.0015000656278712193 88-89 0.004312688680129756 90-91 0.005812754308000975 92-93 0.015750689092647804 94-95 0.0 96-97 9.375410174195122E-4 98-99 0.002437606645290731 100-101 0.0 102-103 0.0 104-105 0.008437869156775609 106-107 0.0 108-109 0.0 110-111 1.875082034839024E-4 112-113 0.006375278918452682 114-115 0.003937672273161951 116-117 3.750164069678048E-4 118-119 0.004312688680129756 120-121 0.0016875738313551216 122-123 1.875082034839024E-4 124-125 0.08025351109111024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 266655.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.393298456807486 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96450443959397 26.532410792972193 2 9.555192947508953 6.763795916071329 3 4.269003369429316 4.532823311019857 4 2.4730339697810932 3.5011531754514262 5 1.6264383648731697 2.878250923477902 6 1.101951726037848 2.3401023794791023 7 0.815868104854945 2.021338433556468 8 0.6442179321452034 1.824079803491403 9 0.4831634491089025 1.539067334195871 >10 3.5092924198436077 24.58532560799535 >50 0.377206552374494 9.114773771352496 >100 0.17270974167708575 10.737844780709157 >500 0.004238275869376337 0.9176651478502185 >1k 0.003178706902032253 2.711368622377229 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4668 1.7505765877257131 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1320 0.4950216571975024 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1242 0.4657703774540136 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 670 0.2512609926684293 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 662 0.24826086141268683 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 604 0.22650990980855415 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 511 0.19163338396054827 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 463 0.17363259642609363 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 442 0.16575725187976975 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 418 0.15675685811254245 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 391 0.1466314151244117 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 385 0.14438131668260487 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 367 0.13763102135718439 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 357 0.13388085728750632 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 347 0.13013069321782827 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 334 0.1252554799272468 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 330 0.1237554142993756 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 312 0.11700511897395512 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 310 0.1162550861600195 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 308 0.1155050533460839 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 297 0.11137987286943803 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 292 0.109504790834599 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 291 0.1091297744276312 No Hit GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC 288 0.10800472520672778 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 284 0.10650465957885656 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 282 0.10575462676492095 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 281 0.10537961035795315 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 277 0.10387954473008193 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 277 0.10387954473008193 No Hit GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT 268 0.10050439706737169 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.750164069678048E-4 0.0 10-11 0.0 0.0 0.0 3.750164069678048E-4 0.0 12-13 0.0 0.0 0.0 7.500328139356096E-4 0.0 14-15 0.0 0.0 0.0 7.500328139356096E-4 0.0 16-17 0.0 0.0 0.0 0.0016875738313551218 0.0 18-19 0.0 0.0 0.0 0.0030001312557424386 0.0 20-21 0.0 0.0 0.0 0.00468770508709756 0.0 22-23 0.0 0.0 0.0 0.009937934784646829 0.0 24-25 0.0 0.0 0.0 0.020438394179745363 0.0 26-27 0.0 0.0 0.0 0.029438787946972682 0.0 28-29 0.0 0.0 0.0 0.04462695242916878 0.0 30-31 0.0 0.0 0.0 0.09731675760814536 0.0 32-33 0.0 0.0 0.0 0.15806941553692974 0.0 34-35 0.0 0.0 0.0 0.22538486058765073 0.0 36-37 0.0 0.0 0.0 0.32963942172470045 0.0 38-39 0.0 0.0 0.0 0.4770208696630478 0.0 40-41 0.0 0.0 0.0 0.6718418930828224 0.0 42-43 0.0 0.0 0.0 0.8237235379047834 0.0 44-45 0.0 0.0 0.0 0.981980461645197 0.0 46-47 0.0 0.0 0.0 1.172113779977874 0.0 48-49 0.0 0.0 0.0 1.3851230991355872 0.0 50-51 0.0 0.0 0.0 1.6240085503740789 0.0 52-53 0.0 0.0 0.0 1.8478933453338584 0.0 54-55 0.0 0.0 0.0 2.065215353171701 0.0 56-57 0.0 0.0 0.0 2.2988505747126435 0.0 58-59 0.0 0.0 0.0 2.559486977555268 0.0 60-61 0.0 0.0 0.0 2.853687348821511 0.0 62-63 0.0 0.0 0.0 3.146200146256399 0.0 64-65 0.0 0.0 0.0 3.4135868444244437 0.0 66-67 0.0 0.0 0.0 3.71660010125443 0.0 68-69 0.0 0.0 0.0 4.038364178432807 0.0 70-71 0.0 0.0 0.0 4.41713074947029 0.0 72-73 0.0 0.0 0.0 4.829836305338358 0.0 74-75 0.0 0.0 0.0 5.221728450619715 0.0 76-77 0.0 0.0 0.0 5.606682792372166 0.0 78-79 0.0 0.0 0.0 6.048077103373272 0.0 80-81 0.0 0.0 0.0 6.54572387541955 0.0 82-83 0.0 0.0 0.0 7.0564962217097005 0.0 84-85 0.0 0.0 0.0 7.565018469558043 0.0 86-87 0.0 0.0 0.0 8.048602126343027 0.0 88-89 0.0 0.0 0.0 8.575875194539762 0.0 90-91 0.0 0.0 0.0 9.190527085559992 0.0 92-93 0.0 0.0 0.0 9.848305863381523 0.0 94-95 0.0 3.750164069678048E-4 0.0 10.53046070765596 0.0 96-97 0.0 3.750164069678048E-4 0.0 11.223491027732464 0.0 98-99 0.0 3.750164069678048E-4 0.0 11.9056458720069 0.0 100-101 0.0 3.750164069678048E-4 0.0 12.690742719993999 0.0 102-103 0.0 3.750164069678048E-4 0.0 13.516341339933621 0.0 104-105 0.0 3.750164069678048E-4 0.0 14.374003862668992 0.0 106-107 0.0 3.750164069678048E-4 0.0 15.196789859556356 0.0 108-109 0.0 3.750164069678048E-4 0.0 15.98976205208978 0.0 110-111 0.0 3.750164069678048E-4 0.0 16.859237591644636 0.0 112-113 0.0 3.750164069678048E-4 0.0 17.760214509384785 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 430 0.0 73.36323 1 GACTGCC 25 0.0020552773 71.39854 7 GTATCAA 955 0.0 64.818794 1 TAAGACT 30 0.004227046 59.521107 4 GATAGTC 40 1.7696813E-4 59.498783 7 GTGGTAC 115 0.0 56.933235 1 GAACAAA 595 0.0 56.019863 1 GTATGGT 75 2.2972017E-8 55.553032 1 CGTCACT 45 3.160965E-4 52.90765 1 GTCCATT 35 0.0077903573 50.998955 6 TGGGAGT 105 1.10958354E-10 50.99895 6 GGATATG 85 6.1783794E-8 48.998997 9 TCCCGCT 135 0.0 48.48049 8 CTCCCGC 140 0.0 46.74904 7 CATATAG 130 1.8189894E-11 45.78547 3 ATCAACG 1150 0.0 45.546585 3 TCAACGC 1165 0.0 45.471058 4 GTACATG 2125 0.0 45.095993 1 GAAAACA 570 0.0 43.85766 1 CGCAGAA 95 1.4776379E-7 43.85766 2 >>END_MODULE