FastQCFastQC Report
Fri 27 May 2016
SRR940135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266655
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69972.6239897995537302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58662.199846243273143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41261.547317695149163No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.49277155875569556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.4683954923027882No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.3667660460145131No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9670.3626408655378673No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT8930.3348896514222497No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7470.2801372560049502No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6920.25951135362172095No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.22838499184339314No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.21788453244829462No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT5070.19013331833267705TruSeq Adapter, Index 3 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.18375803941422436No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4310.16163207140312388No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4290.1608820385891883No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG4230.15863194014738147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3810.14288125105473365No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG3810.14288125105473365No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA3760.14100616901989463No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG3640.13650597213628096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3610.13538092291537754No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT3590.13463089010144194No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA3590.13463089010144194No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC3330.12488046352027901No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA3070.1151300369391161No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT3060.1147550205321483RNA PCR Primer, Index 3 (95% over 23bp)
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT2820.10575462676492095No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC2810.10537961035795315No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC2790.10462957754401754No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT2780.10425456113704974No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC2770.10387954473008193No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT2710.10162944628827511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.0101.478351
GTGGTAT2150.088.690651
GTATGGT701.8189894E-1276.614471
GTATCAA33700.064.893691
TGCGGGC150.004227555459.52753118-119
GCACCGT300.00423309559.4996226
TGCCGTG150.004235414359.49962290-91
GACGGCC150.004238561359.48846414-15
GATAGTC453.1695393E-452.878647
GTATAGA350.007743804251.0763171
GTGGTAC1051.10958354E-1050.9996766
TCTTCGA350.007795696650.990129
AAGCCTC856.177834E-848.999695
GGTGGTA1101.7644197E-1048.6815075
TATCAAC47700.045.847332
ATCAACG48000.045.5522273
TCAACGC48700.044.897474
ATATAAG802.2638014E-644.6917761
CAACGCA49450.044.278795
AACGCAG50350.043.6054846
Produced by FastQC (version 0.11.5)