FastQCFastQC Report
Fri 27 May 2016
SRR940134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251300
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19060.15232158555102693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.066.89231
GTATCAA29300.065.0115361
TCAACGC36400.049.2235764
ATCAACG37950.047.5268363
CAACGCA39100.045.8226745
AACGCAG39250.045.172736
TATCAAC43950.043.070142
GTACATG74800.038.835271
ACGCAGA46800.037.6294827
CGCAGAG48550.036.2731178
TACATGG81050.035.546792
ACATGGG81800.033.6926963
CATGGGA55500.031.7473014
GCAGAGT53750.031.324959
TAAGGTA3800.029.7630964
ATGGGAG22750.029.0423855
GTGTAAG5950.028.0123251
CATGGGG28550.027.9387364
ATATAGG4100.027.5853082
ATGGGAT23150.024.6837395
Produced by FastQC (version 0.11.5)