FastQCFastQC Report
Fri 27 May 2016
SRR940134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251300
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63020.5036362183329338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48950.39119315911452085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.2665228162710781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29400.082.880431
GTATCAA59350.063.7424961
TCAACGC81600.046.4235954
ATCAACG82300.045.9563673
TATCAAC83400.045.4930652
CAACGCA85150.044.5852365
AACGCAG87150.043.4937746
ACGCAGA97550.038.849057
GTACATG73900.038.374131
CGCAGAG99400.038.0661548
GTGGTAT10400.036.08971
ACATGGG78400.035.099243
GCAGAGT107900.034.791749
TACATGG82450.034.0975462
GGTATAG1152.7399583E-531.083471
CATGGGA53500.030.8388234
CATGGGG26700.029.4465524
GAGTACT66900.029.43541112-13
ATGGGAT22850.028.6458385
CAGAGTA105950.028.35689510-11
Produced by FastQC (version 0.11.5)