FastQCFastQC Report
Fri 27 May 2016
SRR940133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793941
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19150.1067482152423073No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG17980.10022626162175903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34100.056.3876231
GGTATCA19950.055.800021
ATCAACG40250.047.180273
CAACGCA41800.045.1434145
TCAACGC42050.045.0190964
AACGCAG42650.044.3672076
TATCAAC44000.043.700412
GTACATG91450.038.3413351
TACATGG98950.034.8937642
ACGCAGA54150.034.6103177
ACATGGG97050.034.2274323
CGCAGAG54500.033.9542128
CATGGGA52100.029.250814
CATGGGG50550.028.145724
GCAGAGT64000.027.7055079
GAGTACA72350.024.8487221
ATGGGAG24650.023.6657665
AGTACAT72250.023.482412
GGACCGA3603.6379788E-1223.1408626
ATGGGGA29050.022.7451215
Produced by FastQC (version 0.11.5)