FastQCFastQC Report
Fri 27 May 2016
SRR940133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793941
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38390.21399811922465675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30890.17219072422114218No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18430.10273470532196989No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG18330.10217727338858969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.074.5530241
GTATCAA56750.061.62861
ATCAACG72750.047.242373
TCAACGC74900.045.9645274
CAACGCA75650.045.544355
TATCAAC76100.045.2409862
AACGCAG78050.044.218896
ACGCAGA92350.037.2990347
CGCAGAG91800.037.1984758
GTACATG85050.034.0464671
GCAGAGT104400.032.253139
ACATGGG88450.031.516423
TACATGG91550.031.4251692
CCGTTGC1352.3340635E-630.8475199
GTGGTAT13900.030.4336191
TGGTATC14100.027.8813422
CATGGGG45650.027.0088634
TAGGACG2705.820766E-1126.4724564
CATGGGA49300.026.2173964
GAGTACT60650.025.99387412-13
Produced by FastQC (version 0.11.5)