FastQCFastQC Report
Fri 27 May 2016
SRR940132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754439
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23540.13417394392167523No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20530.11701746256210675No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA19120.10898070551327234No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18110.10322387954212144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16900.063.7485051
GTATCAA32050.052.5577241
TCAACGC36850.044.580994
ATCAACG37250.044.1022723
CAACGCA40200.040.865915
AACGCAG40950.040.2513246
TATCAAC42050.039.775762
GTACATG90000.037.0359651
TACATGG89600.036.6698572
ACATGGG92850.033.975993
ACGCAGA51550.031.623827
CGCAGAG51550.031.281148
CATGGGG48200.030.6254714
GTAAGGT8900.028.0890734
TAAGGTG10000.027.3801615
CATGGGA51500.027.2761384
TAACGCG808.746054E-626.03200576-77
GAGTACA66400.025.2789571
GCAGAGT61050.025.0489439
TGGTATC10250.024.3895362
Produced by FastQC (version 0.11.5)