##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940132_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1754439 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.210176586361793 33.0 31.0 34.0 28.0 34.0 2 31.341704670267816 33.0 31.0 34.0 28.0 34.0 3 31.530742305660098 34.0 31.0 34.0 28.0 34.0 4 34.83985023132751 37.0 35.0 37.0 32.0 37.0 5 34.919281890108465 37.0 35.0 37.0 32.0 37.0 6 34.90587988525107 37.0 35.0 37.0 32.0 37.0 7 34.79960146804762 37.0 35.0 37.0 32.0 37.0 8 34.81542190979567 37.0 35.0 37.0 32.0 37.0 9 36.410100892650014 39.0 37.0 39.0 32.0 39.0 10-11 36.37219504354383 39.0 37.0 39.0 32.0 39.0 12-13 36.30398007568231 39.0 37.0 39.0 32.0 39.0 14-15 37.69767401431454 41.0 38.0 41.0 32.0 41.0 16-17 37.709454418192934 41.0 38.0 41.0 32.0 41.0 18-19 37.70745577361197 41.0 38.0 41.0 32.0 41.0 20-21 37.73457213388439 41.0 38.0 41.0 32.0 41.0 22-23 37.733405379155386 41.0 38.0 41.0 32.0 41.0 24-25 37.739698558912565 41.0 38.0 41.0 32.0 41.0 26-27 37.69642489707536 40.0 38.0 41.0 32.0 41.0 28-29 37.62786651459527 40.0 38.0 41.0 32.0 41.0 30-31 37.54247682592555 40.0 38.0 41.0 31.0 41.0 32-33 37.453440957479856 40.0 38.0 41.0 31.0 41.0 34-35 37.37567165344592 40.0 38.0 41.0 31.0 41.0 36-37 37.243619755374795 40.0 38.0 41.0 30.0 41.0 38-39 37.08574507292644 40.0 37.5 41.0 30.0 41.0 40-41 36.95886120862566 40.0 37.0 41.0 30.0 41.0 42-43 36.93394697678289 40.0 37.0 41.0 30.0 41.0 44-45 37.004167999001396 40.0 37.0 41.0 30.0 41.0 46-47 36.90836472513436 40.0 37.0 41.0 30.0 41.0 48-49 36.79086591212348 40.0 37.0 41.0 30.0 41.0 50-51 36.64759190829661 40.0 36.0 41.0 30.0 41.0 52-53 36.470125778097724 40.0 36.0 41.0 30.0 41.0 54-55 36.28455420792629 40.0 35.5 41.0 29.5 41.0 56-57 36.07768950644622 39.5 35.0 41.0 29.0 41.0 58-59 35.838529581250754 39.0 35.0 41.0 29.0 41.0 60-61 35.583785472165175 39.0 35.0 41.0 28.0 41.0 62-63 35.294236505230444 38.0 35.0 41.0 28.0 41.0 64-65 34.99352072086861 37.5 34.5 40.0 28.0 41.0 66-67 34.65804282736533 37.0 34.0 40.0 28.0 41.0 68-69 34.31924763414402 36.5 34.0 39.5 27.0 41.0 70-71 34.2164207476008 36.0 34.0 39.0 28.0 41.0 72-73 33.99229468793159 36.0 34.0 39.0 28.0 41.0 74-75 33.6657079556485 35.0 34.0 38.0 28.0 40.5 76-77 33.31130349929522 35.0 34.0 37.0 27.0 39.0 78-79 32.99297068749612 35.0 34.0 37.0 27.0 39.0 80-81 32.691752463323034 35.0 34.0 36.0 26.0 39.0 82-83 32.42001260801886 35.0 34.0 36.0 26.0 37.0 84-85 32.18438315609719 35.0 34.0 36.0 26.0 37.0 86-87 31.98755214629862 35.0 33.5 35.0 26.0 36.5 88-89 31.820165306402785 35.0 33.0 35.0 25.0 36.0 90-91 31.69174790346088 35.0 33.0 35.0 25.0 36.0 92-93 31.560376564816444 35.0 33.0 35.0 25.0 36.0 94-95 31.476219178894222 35.0 33.0 35.0 25.0 36.0 96-97 31.35998088277791 35.0 33.0 35.0 24.0 35.0 98-99 31.28873531653138 35.0 33.0 35.0 24.0 35.0 100-101 31.231627032914794 35.0 33.0 35.0 23.5 35.0 102-103 30.427956742867664 34.0 31.5 35.0 21.5 35.0 104-105 31.04939157189278 35.0 33.0 35.0 23.0 35.0 106-107 31.12717712043565 35.0 33.0 35.0 23.0 35.0 108-109 31.097996282572378 35.0 33.0 35.0 23.0 35.0 110-111 31.01928365705505 35.0 33.0 35.0 21.0 35.0 112-113 30.93021273466903 35.0 33.0 35.0 19.5 35.0 114-115 30.820113722962155 35.0 33.0 35.0 19.0 35.0 116-117 30.753506391501787 35.0 33.0 35.0 18.0 35.0 118-119 30.63527059077004 35.0 33.0 35.0 16.0 35.0 120-121 30.500216308460995 35.0 33.0 35.0 4.5 35.0 122-123 30.284264371688046 35.0 33.0 35.0 2.0 35.0 124-125 28.223024282975924 33.0 28.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3885423048201808 1101 2 0.4487122240170507 1101 3 0.4466335556806875 1101 4 0.5043643257211912 1101 5 0.5147822232357839 1101 6 0.5253605165340076 1101 7 0.498654599743098 1101 8 0.5153578898833544 1101 9 0.5569199218850471 1101 10-11 0.5959270465646398 1101 12-13 0.6135785574671146 1101 14-15 0.6356601916629074 1101 16-17 0.6269673915050689 1101 18-19 0.6225219398967994 1101 20-21 0.6322008346874597 1101 22-23 0.5508070761087325 1101 24-25 0.6165465662235974 1101 26-27 0.7112312961405252 1101 28-29 0.6467221326558104 1101 30-31 0.7613153355133804 1101 32-33 0.7716731983099407 1101 34-35 0.7196134877623734 1101 36-37 0.7271558159888301 1101 38-39 0.8227138024076339 1101 40-41 0.7117658732164145 1101 42-43 0.750400139071786 1101 44-45 0.6817019208474449 1101 46-47 0.6661495831332829 1101 48-49 0.7103186128299299 1101 50-51 0.666619513878473 1101 52-53 0.5604490980443231 1101 54-55 0.6030320501077426 1101 56-57 0.5894396235338277 1101 58-59 0.5688686563183083 1101 60-61 0.5516193594282441 1101 62-63 0.5349878669487822 1101 64-65 0.56013299317366 1101 66-67 0.5196067925549883 1101 68-69 0.4363964008140897 1101 70-71 0.47638682987908254 1101 72-73 0.5567960290728706 1101 74-75 0.5479339936797984 1101 76-77 0.6265735865912561 1101 78-79 0.6033934495713922 1101 80-81 0.6079499707008011 1101 82-83 0.5595963119826379 1101 84-85 0.5485670843828316 1101 86-87 0.5152904222844441 1101 88-89 0.4609182956632232 1101 90-91 0.4226431976914178 1101 92-93 0.42924824948102014 1101 94-95 0.43682249296115927 1101 96-97 0.43859176145656065 1101 98-99 0.44559941072474984 1101 100-101 0.3951620005915153 1101 102-103 0.3113440051577854 1101 104-105 0.3623934421087682 1101 106-107 0.45319557930783816 1101 108-109 0.3704739095120999 1101 110-111 0.32753819908251103 1101 112-113 0.3579649576988331 1101 114-115 0.30906262799758366 1101 116-117 0.3153513085795723 1101 118-119 0.37337040353941475 1101 120-121 0.25208250534432963 1101 122-123 0.25473238572252654 1101 124-125 0.09701316329179832 1102 1 -8.540793290272575E-4 1102 2 0.1468059121284604 1102 3 0.13035088555494667 1102 4 0.09795621978976499 1102 5 -0.04947655152236763 1102 6 0.1341840340974656 1102 7 0.1642858583247957 1102 8 0.23169196888817822 1102 9 0.21545929615935222 1102 10-11 0.2648940720605353 1102 12-13 0.16007051348044854 1102 14-15 0.3346908755435365 1102 16-17 0.24081799401774617 1102 18-19 0.2670779596162447 1102 20-21 0.2731593949165685 1102 22-23 0.36987640806290045 1102 24-25 0.16802818933112462 1102 26-27 0.042152869246031344 1102 28-29 0.2134476194675301 1102 30-31 0.31561042495831515 1102 32-33 0.033961206406452504 1102 34-35 0.1722582871143601 1102 36-37 0.16106774969710358 1102 38-39 0.06255652036625037 1102 40-41 0.16766130030520543 1102 42-43 0.16575188705114385 1102 44-45 0.0817290817169507 1102 46-47 0.197496759162064 1102 48-49 0.2610328212958777 1102 50-51 0.03324105187471815 1102 52-53 0.18233245411758503 1102 54-55 0.2386354412964593 1102 56-57 0.20742145162612502 1102 58-59 0.014459066687592781 1102 60-61 0.1460546658936508 1102 62-63 0.1891696492410091 1102 64-65 0.14500877364194054 1102 66-67 0.1242098394684561 1102 68-69 0.04778664378689257 1102 70-71 0.11912277695675044 1102 72-73 -0.027278482504044632 1102 74-75 0.09190694560614077 1102 76-77 0.18050604046813135 1102 78-79 0.09439271236992397 1102 80-81 0.08828963805839862 1102 82-83 0.03717255841555556 1102 84-85 0.022295799201138777 1102 86-87 0.14605947308564993 1102 88-89 0.1513515494920057 1102 90-91 0.14572829441540947 1102 92-93 0.08068799248000147 1102 94-95 0.16054175647860802 1102 96-97 0.04107911143265497 1102 98-99 0.11740934103612588 1102 100-101 0.11253525787234864 1102 102-103 0.01049205560232025 1102 104-105 0.07490942797705813 1102 106-107 0.09508749423125806 1102 108-109 0.1452668408067197 1102 110-111 0.09815280323259401 1102 112-113 0.1223543308420787 1102 114-115 0.08799832785401662 1102 116-117 0.0027052201321318137 1102 118-119 -0.0025375023652962625 1102 120-121 0.09756551175045303 1102 122-123 0.0072392270698955485 1102 124-125 -0.12208805518230292 1103 1 0.15277071175382417 1103 2 0.24387081965628354 1103 3 0.35395269230325255 1103 4 0.2884828365931895 1103 5 0.19200822606841683 1103 6 0.2917054958999188 1103 7 0.09389813445434214 1103 8 0.23874373681842798 1103 9 0.3099647866231052 1103 10-11 0.33205183642252223 1103 12-13 0.3314218844642838 1103 14-15 0.44402377507910984 1103 16-17 0.17441973333634309 1103 18-19 0.3178107672635022 1103 20-21 0.41519868988383024 1103 22-23 0.29325335206896597 1103 24-25 0.3518090004920893 1103 26-27 0.3087736786047799 1103 28-29 0.18558114438296514 1103 30-31 0.30001255487881195 1103 32-33 0.3651170710897489 1103 34-35 0.540993339557815 1103 36-37 0.5296640348720345 1103 38-39 0.3816081399511475 1103 40-41 0.3641893141295256 1103 42-43 0.4343471607805043 1103 44-45 0.3919126989344548 1103 46-47 0.3699109908756597 1103 48-49 0.4149198986074083 1103 50-51 0.3209701941044969 1103 52-53 0.29959592986756434 1103 54-55 0.36185648475889565 1103 56-57 0.4273794490497025 1103 58-59 0.4926244086159599 1103 60-61 0.3624267056146522 1103 62-63 0.4424729385539763 1103 64-65 0.4474357526348314 1103 66-67 0.4093258507439259 1103 68-69 0.41462155778490484 1103 70-71 0.3841746196726703 1103 72-73 0.18981934604489936 1103 74-75 0.34430054689491385 1103 76-77 0.28566933222696633 1103 78-79 0.4185296044796374 1103 80-81 0.33190220597466435 1103 82-83 0.2691751869480612 1103 84-85 0.3676964190027334 1103 86-87 0.28475126630817016 1103 88-89 0.3171418087806792 1103 90-91 0.37788340603149706 1103 92-93 0.35742318100886195 1103 94-95 0.3294263496184975 1103 96-97 0.11404165392486121 1103 98-99 0.24689061963819725 1103 100-101 0.12107538329772893 1103 102-103 0.2144814728165727 1103 104-105 0.3324022474796884 1103 106-107 0.354870548349421 1103 108-109 0.39763423646001783 1103 110-111 0.43632472875219364 1103 112-113 0.42278485418547973 1103 114-115 0.3177663512778679 1103 116-117 0.26692928659084814 1103 118-119 0.4777567026338829 1103 120-121 0.358899602339811 1103 122-123 0.15395676434089722 1103 124-125 0.3173419094140364 1104 1 -0.013712013001214274 1104 2 0.1275803830758271 1104 3 0.12178128234595675 1104 4 0.070246393851491 1104 5 0.06969055190449325 1104 6 0.05873734195755986 1104 7 0.11135175040186596 1104 8 0.0227721801532752 1104 9 0.07605372780443531 1104 10-11 0.15974068024429755 1104 12-13 0.1272373984249242 1104 14-15 0.11192123698459255 1104 16-17 0.1680757884994719 1104 18-19 0.16360814025699 1104 20-21 0.13830022399584152 1104 22-23 0.14627718085227315 1104 24-25 0.1333376671705011 1104 26-27 0.024292394795580208 1104 28-29 0.13097911222999414 1104 30-31 0.14735965873570223 1104 32-33 0.0845180909949832 1104 34-35 0.026500773100188724 1104 36-37 0.15365131613967975 1104 38-39 0.06491410483485538 1104 40-41 0.21113591891229788 1104 42-43 0.1551847963895483 1104 44-45 0.12816916906080422 1104 46-47 -0.10067139494412203 1104 48-49 -0.016165013273685247 1104 50-51 0.04757358819741597 1104 52-53 -0.04483076695098731 1104 54-55 -0.001804727057709954 1104 56-57 -0.04079371301507706 1104 58-59 -0.018264653748239823 1104 60-61 0.00200465920416093 1104 62-63 -0.020361552620755674 1104 64-65 -0.06079058343068766 1104 66-67 0.13680955274101336 1104 68-69 0.14640029328960225 1104 70-71 0.1091701679948045 1104 72-73 0.0921658664736924 1104 74-75 0.08381297117840347 1104 76-77 -0.21691218513579713 1104 78-79 1.6973023269883925E-4 1104 80-81 -0.04557500842361151 1104 82-83 0.024491067678347633 1104 84-85 0.01814064479669497 1104 86-87 0.10237741895848984 1104 88-89 0.1426048896334784 1104 90-91 0.12467880570100931 1104 92-93 0.16020192441414594 1104 94-95 0.12524672547989013 1104 96-97 0.21505706189606855 1104 98-99 0.17577635707003125 1104 100-101 0.1160500011371397 1104 102-103 0.005618394880912092 1104 104-105 0.10509896758066972 1104 106-107 0.09824643648194709 1104 108-109 0.0526879999662242 1104 110-111 0.05154471152890849 1104 112-113 0.11725313544886617 1104 114-115 0.2528176625628866 1104 116-117 0.23362237971387145 1104 118-119 0.253252434188898 1104 120-121 0.1662719303455127 1104 122-123 0.23715695249645563 1104 124-125 0.2645401479473932 1105 1 0.03768055432938766 1105 2 0.16560115972084688 1105 3 0.15366037146300826 1105 4 0.271849388203826 1105 5 0.09602537273918443 1105 6 0.27779005755455444 1105 7 0.2726108054152263 1105 8 0.25537990306684577 1105 9 0.3883528244194636 1105 10-11 0.37138975085828463 1105 12-13 0.36745254191438903 1105 14-15 0.4023108293124764 1105 16-17 0.3519980756235128 1105 18-19 0.30190404418616623 1105 20-21 0.31759916339527905 1105 22-23 0.33059311162903526 1105 24-25 0.28302172430023376 1105 26-27 0.30397137265237006 1105 28-29 0.24552881049699238 1105 30-31 0.16115290814008887 1105 32-33 0.20347950061415077 1105 34-35 0.21664789061279066 1105 36-37 0.014580823087399608 1105 38-39 0.20545123794808262 1105 40-41 0.224212023351825 1105 42-43 0.19992079513181693 1105 44-45 0.26091778133088184 1105 46-47 0.15924588546802454 1105 48-49 0.22789435375216271 1105 50-51 0.29265697292814963 1105 52-53 0.2868608728076225 1105 54-55 0.14267761037532267 1105 56-57 0.15995668909403093 1105 58-59 0.17511203480767534 1105 60-61 0.1639884176242674 1105 62-63 0.2556769772925307 1105 64-65 0.3423582634534199 1105 66-67 0.2113947454510452 1105 68-69 0.20769018674580764 1105 70-71 0.3261576149345373 1105 72-73 0.2539001963120029 1105 74-75 0.24760440655674643 1105 76-77 0.25188989252870186 1105 78-79 0.29110987946658184 1105 80-81 0.2467691969725081 1105 82-83 0.1931171848160531 1105 84-85 0.17349655670758324 1105 86-87 0.15359946784043643 1105 88-89 0.07678839650863623 1105 90-91 0.17198104662785596 1105 92-93 0.2749656777549774 1105 94-95 0.24870480333788691 1105 96-97 0.3692683233697842 1105 98-99 0.36347726045500295 1105 100-101 0.3350942512792372 1105 102-103 0.4179471205880816 1105 104-105 0.3170240724918436 1105 106-107 0.28565800271203656 1105 108-109 0.2888161427663576 1105 110-111 0.28257634814321264 1105 112-113 0.3443589173545618 1105 114-115 0.3394549420075528 1105 116-117 0.3601703486093726 1105 118-119 0.3253892964075682 1105 120-121 0.35297784044238156 1105 122-123 0.3982424656844259 1105 124-125 0.5008675312752864 1106 1 -0.07152105826328992 1106 2 -0.11475432750785686 1106 3 -0.14173260173252444 1106 4 -0.0032109655357359657 1106 5 0.06885522999333915 1106 6 0.04173954702856264 1106 7 -0.10818485681710399 1106 8 0.06520578808223121 1106 9 -0.1640642873097491 1106 10-11 0.004933026340893321 1106 12-13 0.148063218083621 1106 14-15 0.09853216684020794 1106 16-17 0.14554363195910014 1106 18-19 0.11487824685031711 1106 20-21 0.0426835105233252 1106 22-23 0.27240825590039464 1106 24-25 0.16355590303024314 1106 26-27 0.22222426889133118 1106 28-29 0.12112827494087242 1106 30-31 0.2565278122405914 1106 32-33 0.3214015250637132 1106 34-35 0.3169386993677108 1106 36-37 0.3681980485630447 1106 38-39 0.2593530516705229 1106 40-41 0.3432295977683566 1106 42-43 0.40455670286686995 1106 44-45 0.2228158360891257 1106 46-47 0.2736381418240512 1106 48-49 0.2637398837246465 1106 50-51 0.2502078552035485 1106 52-53 0.19228508184039583 1106 54-55 0.19752931049943356 1106 56-57 0.37073690373118495 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0.2919359819226699 2204 38-39 0.31481934022422564 2204 40-41 0.2909466739848696 2204 42-43 0.22272279403701134 2204 44-45 -3.3312365049198434E-4 2204 46-47 0.1103446652882667 2204 48-49 0.18652390293900822 2204 50-51 0.24532556433567976 2204 52-53 0.2220046103365334 2204 54-55 0.21330323944773255 2204 56-57 0.1081604114369199 2204 58-59 0.17632603420729254 2204 60-61 0.20286994894104282 2204 62-63 0.16829065178176705 2204 64-65 0.16936511482457206 2204 66-67 0.2618353168695293 2204 68-69 0.0034844513711931313 2204 70-71 0.12860194754389198 2204 72-73 0.14017317400973184 2204 74-75 0.13399172092520217 2204 76-77 0.07951566286650547 2204 78-79 0.05364806526587529 2204 80-81 0.11291406462450482 2204 82-83 0.12165499979371219 2204 84-85 0.14737084817339507 2204 86-87 0.10652277839472646 2204 88-89 0.14254655017006357 2204 90-91 0.22931492736455183 2204 92-93 0.04133171402486724 2204 94-95 0.07070821136529304 2204 96-97 0.04111898820000093 2204 98-99 0.1099729960062028 2204 100-101 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-1.6049295085355908 2216 110-111 -1.7048445567134145 2216 112-113 -1.6613367515468234 2216 114-115 -1.6748662927361906 2216 116-117 -1.7111534155418013 2216 118-119 -1.7700045626168475 2216 120-121 -1.9180129669215482 2216 122-123 -2.047043750272941 2216 124-125 -2.2965688856430972 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30650.0 3 5445.0 4 3588.0 5 2983.0 6 3191.0 7 3667.0 8 3965.0 9 4324.0 10 4897.0 11 5576.0 12 6113.0 13 6164.0 14 6173.0 15 5733.0 16 5305.0 17 5249.0 18 4666.0 19 4653.0 20 4635.0 21 4738.0 22 5294.0 23 6628.0 24 8920.0 25 12817.0 26 16478.0 27 16938.0 28 18670.0 29 23851.0 30 26900.0 31 31535.0 32 40357.0 33 53515.0 34 77233.0 35 125474.0 36 283048.0 37 597259.0 38 286115.0 39 1691.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.09961204181169 18.159122718500846 12.613401518196406 24.127863721491057 2 15.543351487423058 19.79920672290368 39.73528167721444 24.922160112458826 3 19.18651414590712 24.821961256443625 28.623636385401653 27.367888212247603 4 12.438170361572599 15.905191608445838 36.821641556587345 34.83499647339423 5 13.531111521628569 38.748229643465585 33.7879814172431 13.932677417662742 6 32.83471215259427 35.93350653378464 17.448605702160215 13.783175611460877 7 29.75853203896778 32.39826252515291 20.240099870601448 17.603105565277865 8 27.395290300737223 34.79915322539275 19.523922978622018 18.281633495248006 9 27.51805481686952 15.96969857619335 19.005427418467587 37.50681918846954 10-11 25.430281218900223 26.482871398981096 27.055221228042775 21.0316261540759 12-13 26.182039447152754 23.61918577725084 27.271887811738953 22.92688696385745 14-15 22.823178987273216 25.043966536976072 24.767084241880085 27.365770233870624 16-17 23.18994601600881 28.5797382480368 25.41060835871225 22.81970737724214 18-19 23.368653324741086 27.320099333378746 26.783188341524184 22.52805900035598 20-21 24.058163080636863 26.528612000552904 26.660254135726202 22.752970783084027 22-23 23.739805099203103 26.45108300069937 26.581638149348706 23.227473750748818 24-25 23.310377613655895 26.704115185403797 26.701749595783436 23.28375760515687 26-27 23.050712593676828 27.153085094013118 26.380671623245306 23.41553068906475 28-29 23.09896952582684 27.316001819489742 26.36666936474043 23.218359289942985 30-31 23.100014278267764 26.840293442627157 26.4947365631098 23.56495571599528 32-33 22.885513013214137 27.032066068972515 26.427275509872555 23.655145407940793 34-35 23.534135928129547 26.991232787544934 26.155721401967412 23.318909882358106 36-37 23.61166885886472 26.58759553162109 26.14420625567347 23.656529353840718 38-39 23.202623320098176 26.788427843307584 26.271019791178613 23.737929045415633 40-41 23.536346291206993 26.621073831548216 26.362013267412408 23.480566609832387 42-43 23.269456695162056 26.62380673070291 26.339764888825805 23.76697168530923 44-45 23.0919509732366 26.852432656883618 26.515400020348494 23.54021634953129 46-47 23.443047210789736 27.081589958158997 25.981724487818227 23.493638343233044 48-49 23.227651234252995 26.9593092948453 26.139789475604214 23.67324999529749 50-51 23.431755141407955 26.9014288434155 26.261168361373233 23.405647653803314 52-53 23.68811806410168 26.616692383415348 26.31530196897004 23.379887583512932 54-55 23.03563112711918 26.94949193253735 26.454163918802685 23.56071302154078 56-57 23.150093656763637 27.066804691160694 26.333483062547668 23.449618589527997 58-59 23.36796079007039 26.552157101954084 26.28017313253304 23.79970897544249 60-61 22.940652746261833 26.7754045108222 26.703357210401485 23.58058553251448 62-63 23.015551814967434 26.856479029874098 26.776761941460236 23.35120721369823 64-65 22.843735019901263 26.865683126701782 26.601719893457123 23.688861959939832 66-67 22.8635739627837 27.050742425058544 26.335035605794392 23.75064800636336 68-69 23.216005845759945 26.597039273064706 26.725684750751526 23.461270130423827 70-71 22.99970416444125 26.623005746277972 26.64537866666515 23.73191142261563 72-73 22.970399175799514 26.8074913326427 26.451563259239837 23.770546232317947 74-75 22.941572297808197 27.15446148829995 26.480529181930084 23.423437031961765 76-77 23.09560340677473 26.707655666181452 26.561635561980733 23.635105365063087 78-79 23.16723147965804 26.788238020012933 26.555111944690413 23.489418555638615 80-81 23.253826227797248 27.037346422921253 26.390163086894653 23.318664262386843 82-83 23.028434321138455 27.013160823444217 26.52578941713503 23.432615438282294 84-85 23.0852404503553 26.975690777155293 26.554900517943935 23.384168254545475 86-87 23.103239147931045 26.955355982517222 26.462911790389033 23.478493079162703 88-89 22.981236526471672 27.097253366743313 26.36089048693374 23.560619619851273 90-91 22.977738738266297 27.144443405755304 26.550718557549587 23.32709929842882 92-93 22.990660172567907 26.576409410970648 26.783596928072384 23.64933348838906 94-95 22.745162413740232 26.64780593682653 26.917949270393553 23.68908237903968 96-97 23.043035157937698 26.584392844155296 26.80323935007803 23.56933264782898 98-99 22.834404879398697 26.68205277427309 26.51886275422916 23.96467959209905 100-101 22.540738093487434 26.669579278618404 27.019833690427536 23.76984893746662 102-103 22.623240389937056 26.32585268094551 27.07102050401354 23.979886425103896 104-105 22.479915134531833 26.53881363912567 26.884420391881463 24.096850834461033 106-107 22.71310088296031 26.635123820206914 26.65313527572061 23.99864002111216 108-109 22.77289207547256 26.35090191223519 26.643588064332814 24.232617947959433 110-111 22.626034344439013 26.615102034418538 26.874701648538775 23.884161972603675 112-113 22.49735228364823 26.816607024172022 27.006364779501375 23.679675912678373 114-115 22.47886376623247 26.838720525766043 26.75273634605711 23.929679361944377 116-117 22.338825030145006 26.792477242693025 26.97310503970933 23.895592687452638 118-119 22.224160264713806 26.92932842552573 26.600288711329995 24.24622259843047 120-121 22.29763524826687 26.994335710672374 26.890825276267698 23.817203764793057 122-123 22.22496131233132 26.994189012417102 26.87990720659358 23.900942468658002 124-125 22.279165584064632 26.94054810703172 26.669571985501168 24.110714323402487 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 295.0 1 276.5 2 259.5 3 233.5 4 198.5 5 174.5 6 161.5 7 162.5 8 169.0 9 176.5 10 163.5 11 159.5 12 169.0 13 183.0 14 222.0 15 256.5 16 297.5 17 385.0 18 485.5 19 571.5 20 749.0 21 1074.0 22 1550.5 23 2198.5 24 3118.5 25 4344.0 26 5725.5 27 7706.5 28 9890.5 29 12619.5 30 16699.5 31 20462.5 32 25491.5 33 28723.5 34 30379.5 35 32996.5 36 36760.0 37 41191.0 38 44074.5 39 46603.0 40 49134.0 41 52598.5 42 55988.0 43 59952.0 44 63013.5 45 65293.5 46 66508.0 47 67106.5 48 68602.0 49 68042.0 50 66650.0 51 64438.5 52 62536.0 53 63268.0 54 65062.5 55 62201.5 56 55704.0 57 51701.5 58 49792.5 59 45642.0 60 37766.0 61 30783.0 62 25249.5 63 18974.0 64 14361.0 65 11643.0 66 8786.5 67 7022.0 68 5462.5 69 3724.5 70 2628.0 71 1850.0 72 1406.5 73 1094.5 74 883.5 75 734.5 76 537.5 77 342.0 78 206.0 79 133.5 80 86.0 81 56.0 82 46.5 83 38.0 84 26.0 85 23.5 86 21.5 87 18.5 88 13.5 89 7.5 90 10.0 91 10.0 92 9.0 93 9.0 94 6.5 95 7.0 96 3.5 97 2.0 98 4.0 99 4.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021887338345761807 2 0.011228660557591344 3 0.010601679511228377 4 0.03579491791963129 5 0.03163404370285886 6 0.022913307330719394 7 0.009632708803212878 8 9.689707080154967E-4 9 0.011285658834533432 10-11 2.8499138471044016E-4 12-13 0.003790385416648855 14-15 0.015047545112711244 16-17 0.0017099483082626413 18-19 0.008236251018131722 20-21 0.0035053940319384144 22-23 4.5598621553670434E-4 24-25 0.006811294094579521 26-27 1.7099483082626412E-4 28-29 0.005756825971150892 30-31 0.001396457785081157 32-33 0.0017099483082626413 34-35 2.564922462393962E-4 36-37 0.006355307879042817 38-39 0.0011969638157838488 40-41 0.012311627819491015 42-43 0.006839793233050565 44-45 5.414836309498364E-4 46-47 0.010202691572633758 48-49 0.0033059000626411063 50-51 0.00872073637213947 52-53 0.003989879385946163 54-55 0.0026219207393360497 56-57 0.009176722587676175 58-59 0.016614997728618665 60-61 0.0020519379699151697 62-63 0.006269810463629685 64-65 0.001595951754378465 66-67 0.0011114664003707168 68-69 2.8499138471044016E-4 70-71 0.004901851817019571 72-73 6.554801848340124E-4 74-75 0.006184313048216553 76-77 0.013024106281267118 78-79 0.001339459508139069 80-81 0.0038473836935909427 82-83 0.0015674526159074212 84-85 1.1399655388417608E-4 86-87 0.001538953477436377 88-89 0.004160874216772427 90-91 0.005186843201730012 92-93 0.013166601973622337 94-95 0.0 96-97 7.409776002471446E-4 98-99 0.0024224267700387416 100-101 0.0 102-103 1.1399655388417608E-4 104-105 0.00758077083329771 106-107 0.0 108-109 0.0 110-111 3.704888001235723E-4 112-113 0.00552883286338254 114-115 0.002650419877807094 116-117 1.4249569235522008E-4 118-119 0.005215342340201055 120-121 0.001510454338965333 122-123 2.2799310776835217E-4 124-125 0.07595020402533231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1754439.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.496065737279295 #Duplication Level Percentage of deduplicated Percentage of total 1 71.796580828139 34.818517035533155 2 14.135558976818857 13.710379947459916 3 5.538630738251948 8.058054011303469 4 2.705955980401751 5.249128764309894 5 1.5392827002007883 3.73245775085971 6 0.9428829640278006 2.743566852363178 7 0.6440086592089288 2.186232039066132 8 0.4568802976340952 1.7725517558504649 9 0.3305072468514315 1.4425471052958796 >10 1.7315834248469855 15.033642003092595 >50 0.11409924220393483 3.808661978159246 >100 0.05814451110553168 4.803487942626459 >500 0.004354478428576087 1.4967746666071373 >1k 0.0015299518803105173 1.1439981474727725 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2354 0.13417394392167523 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2053 0.11701746256210675 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1912 0.10898070551327234 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1811 0.10322387954212144 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 5.699827694208804E-5 0.0 7 0.0 0.0 0.0 5.699827694208804E-5 0.0 8 5.699827694208804E-5 0.0 0.0 5.699827694208804E-5 0.0 9 5.699827694208804E-5 0.0 0.0 5.699827694208804E-5 0.0 10-11 5.699827694208804E-5 0.0 0.0 5.699827694208804E-5 0.0 12-13 5.699827694208804E-5 0.0 0.0 5.699827694208804E-5 0.0 14-15 5.699827694208804E-5 0.0 0.0 5.699827694208804E-5 8.549741541313206E-5 16-17 5.699827694208804E-5 0.0 0.0 5.699827694208804E-5 1.1399655388417608E-4 18-19 8.549741541313206E-5 0.0 0.0 5.699827694208804E-5 1.7099483082626412E-4 20-21 1.1399655388417608E-4 0.0 0.0 1.1399655388417608E-4 1.7099483082626412E-4 22-23 1.1399655388417608E-4 0.0 0.0 2.2799310776835217E-4 2.2799310776835217E-4 24-25 1.1399655388417608E-4 0.0 0.0 5.984819078919245E-4 2.849913847104402E-4 26-27 1.1399655388417608E-4 0.0 0.0 0.0010829672618996727 2.849913847104402E-4 28-29 1.1399655388417608E-4 0.0 0.0 0.0021374353853283014 2.849913847104402E-4 30-31 1.1399655388417608E-4 0.0 0.0 0.005072846647845835 3.1349052318148423E-4 32-33 1.1399655388417608E-4 0.0 0.0 0.011570650219243871 3.9898793859461626E-4 34-35 1.1399655388417608E-4 0.0 0.0 0.02020588917597021 4.5598621553670434E-4 36-37 1.1399655388417608E-4 0.0 0.0 0.03733387139706767 4.5598621553670434E-4 38-39 2.2799310776835217E-4 0.0 0.0 0.06785644869955582 4.5598621553670434E-4 40-41 2.2799310776835217E-4 0.0 0.0 0.1037083648961292 4.5598621553670434E-4 42-43 2.564922462393962E-4 0.0 0.0 0.13785033278443992 4.8448535400774835E-4 44-45 2.849913847104402E-4 0.0 0.0 0.17840460682873555 5.129844924787924E-4 46-47 3.1349052318148423E-4 0.0 0.0 0.2259981680753791 5.129844924787924E-4 48-49 3.4198966165252824E-4 0.0 0.0 0.27732511646172936 6.839793233050565E-4 50-51 3.4198966165252824E-4 0.0 0.0 0.3400802193749683 6.839793233050565E-4 52-53 3.4198966165252824E-4 0.0 0.0 0.39930142911779776 7.694767387181886E-4 54-55 3.4198966165252824E-4 0.0 0.0 0.4631110001544653 8.549741541313207E-4 56-57 3.4198966165252824E-4 0.0 0.0 0.534102354085836 8.549741541313207E-4 58-59 3.4198966165252824E-4 0.0 0.0 0.6160088780516165 8.549741541313207E-4 60-61 3.4198966165252824E-4 0.0 0.0 0.7097710436213513 8.549741541313207E-4 62-63 3.4198966165252824E-4 0.0 0.0 0.8084350610081057 9.119724310734087E-4 64-65 3.4198966165252824E-4 0.0 0.0 0.9029097050396167 9.119724310734087E-4 66-67 3.4198966165252824E-4 0.0 0.0 1.0137428545535068 9.119724310734087E-4 68-69 3.4198966165252824E-4 0.0 0.0 1.1408205129958922 9.119724310734087E-4 70-71 3.7048880012357225E-4 0.0 0.0 1.284456170889954 9.404715695444526E-4 72-73 4.8448535400774835E-4 0.0 0.0 1.4387790057106573 0.0010829672618996727 74-75 5.129844924787924E-4 0.0 0.0 1.588741472345291 0.0010829672618996727 76-77 5.129844924787924E-4 0.0 0.0 1.7497331055682186 0.0010829672618996727 78-79 5.129844924787924E-4 0.0 0.0 1.9407913298780977 0.0010829672618996727 80-81 5.129844924787924E-4 0.0 0.0 2.159807209028071 0.001168464677312805 82-83 5.129844924787924E-4 0.0 0.0 2.3977465161228175 0.001367958646610113 84-85 5.414836309498364E-4 0.0 0.0 2.6289030282614556 0.001367958646610113 86-87 5.699827694208804E-4 0.0 0.0 2.8606010240310438 0.001367958646610113 88-89 5.699827694208804E-4 0.0 0.0 3.1231635867647722 0.001367958646610113 90-91 5.699827694208804E-4 0.0 0.0 3.4245704752345336 0.001367958646610113 92-93 5.699827694208804E-4 0.0 0.0 3.754334006482984 0.001424956923552201 94-95 5.699827694208804E-4 0.0 0.0 4.081675110961395 0.0015389534774363771 96-97 5.699827694208804E-4 0.0 0.0 4.40935820510146 0.0015389534774363771 98-99 6.269810463629685E-4 0.0 0.0 4.76177855143439 0.0015389534774363771 100-101 6.554801848340124E-4 0.0 0.0 5.182311838713115 0.0015389534774363771 102-103 6.839793233050565E-4 0.0 0.0 5.624903459168429 0.001595951754378465 104-105 8.549741541313207E-4 0.0 0.0 6.075246845287866 0.001595951754378465 106-107 8.549741541313207E-4 0.0 0.0 6.515587033803968 0.001595951754378465 108-109 8.549741541313207E-4 0.0 0.0 6.97861253654302 0.0017099483082626413 110-111 8.834732926023647E-4 2.849913847104402E-5 0.0 7.489402595359542 0.0019379414160309934 112-113 9.119724310734087E-4 5.699827694208804E-5 0.0 8.05191859050101 0.0020519379699151697 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1690 0.0 63.748505 1 GTATCAA 3205 0.0 52.557724 1 TCAACGC 3685 0.0 44.58099 4 ATCAACG 3725 0.0 44.102272 3 CAACGCA 4020 0.0 40.86591 5 AACGCAG 4095 0.0 40.251324 6 TATCAAC 4205 0.0 39.77576 2 GTACATG 9000 0.0 37.035965 1 TACATGG 8960 0.0 36.669857 2 ACATGGG 9285 0.0 33.97599 3 ACGCAGA 5155 0.0 31.62382 7 CGCAGAG 5155 0.0 31.28114 8 CATGGGG 4820 0.0 30.625471 4 GTAAGGT 890 0.0 28.089073 4 TAAGGTG 1000 0.0 27.380161 5 CATGGGA 5150 0.0 27.276138 4 TAACGCG 80 8.746054E-6 26.032005 76-77 GAGTACA 6640 0.0 25.278957 1 GCAGAGT 6105 0.0 25.048943 9 TGGTATC 1025 0.0 24.389536 2 >>END_MODULE