FastQCFastQC Report
Fri 27 May 2016
SRR940132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754439
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38910.22178029558166454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31780.18114052412195578No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22320.1272201541347405No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA19940.11365456422252355No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19680.11217260902202926No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA18250.10402185541931067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.10088695018749584No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.076.4110951
GTATCAA59100.058.9716221
ATCAACG75100.046.0707243
TCAACGC75600.045.844794
TATCAAC78300.044.3412552
CAACGCA78200.044.2864275
AACGCAG81400.042.6185386
GTACATG93100.038.5231251
TACATGG93850.037.628912
ACGCAGA93750.036.995827
CGCAGAG94400.036.615048
ACATGGG94550.036.02663
CATGGGG47250.032.894824
TGGTATC12600.031.6668932
GCAGAGT108300.031.3113529
CATGGGA53150.029.2432794
GAGTACT60400.028.21909312-13
TAAGGTG10100.027.1013475
GTAAGGT9250.026.3955614
ATGGGGA25150.026.2627035
Produced by FastQC (version 0.11.5)