FastQCFastQC Report
Fri 27 May 2016
SRR940131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084491
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA13040.12024073966496725No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG11930.11000552332845547No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA11640.10733145779909653No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA10850.10004693446049806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13900.061.2456971
GTATCAA23850.055.663541
TCAACGC28100.046.397244
ATCAACG28300.046.0693443
CAACGCA29250.044.369545
AACGCAG30200.043.1510286
TATCAAC31150.041.47212
GTACATG62650.035.6341
ACGCAGA37700.034.4055947
TACATGG65150.034.1752362
CGCAGAG38700.033.5165638
ACATGGG66450.032.0732043
CGCACGA400.00536644629.74636328-29
GCAGAGT42750.028.3927869
CATGGGG33750.027.5172584
CATGGGA36150.026.843164
TATACGG1358.2151615E-526.4589025
TGTACGC1150.001001576425.8837072
ATGGGAG17450.024.9047245
GAGTACA49300.024.1511271
Produced by FastQC (version 0.11.5)