FastQCFastQC Report
Fri 27 May 2016
SRR940131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084491
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.31507868668343025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29520.27220142905750255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.16219590572904707No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA13260.12226934110103264No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG11960.11028215079700984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.081.320721
GTATCAA42550.058.5313151
ATCAACG55800.044.3986973
TATCAAC56550.043.9192242
TCAACGC56550.043.7045444
TGGTATC8850.043.071292
CAACGCA58900.042.0250745
AACGCAG59950.041.3882756
GTGGTAT10450.038.2005921
GTACATG57750.035.594161
ACGCAGA70200.035.2554747
CGCAGAG72150.034.0552528
TACATGG61150.032.726082
ACATGGG61250.031.6000943
GCAGAGT76600.031.3001679
CATGGGG31900.028.0034564
GAGTACT44550.027.84381312-13
GTACTTT45850.026.92459114-15
CAGAGTA74450.026.88999610-11
AGTACTT44600.025.87838712-13
Produced by FastQC (version 0.11.5)