FastQCFastQC Report
Fri 27 May 2016
SRR940130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709537
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10400.14657445630037616No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA8540.12036017853896273No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG7170.10105181266093241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10300.065.305861
GTATCAA17650.054.6362951
ATCAACG21100.043.7280543
GTACATG51100.043.450911
TATCGTC558.52817E-443.2707027
TCAACGC21700.042.518994
TACATGG52600.041.5328062
CAACGCA22350.041.2824175
TATCAAC23900.039.3523032
AACGCAG23600.039.3342136
ACATGGG54250.038.1847953
TAAGGTG4150.037.2937625
GTAAGGT3700.037.0030144
CATGGGG19950.034.015194
CATGGGA35250.033.098474
ATGGGAG14450.032.1319965
ACGCAGA28850.031.9655767
AATAACG750.003910382731.7318467
CGCAGAG30000.030.7423958
GGTGCGT900.00952872426.4432077
Produced by FastQC (version 0.11.5)