##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940130_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 709537 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.983096018953205 33.0 31.0 34.0 27.0 34.0 2 31.006030693254896 33.0 31.0 34.0 27.0 34.0 3 31.07537168604315 34.0 31.0 34.0 27.0 34.0 4 34.30830245639058 37.0 35.0 37.0 30.0 37.0 5 34.35380959696253 37.0 35.0 37.0 30.0 37.0 6 34.35753315190046 37.0 35.0 37.0 30.0 37.0 7 34.225228564542796 37.0 35.0 37.0 30.0 37.0 8 34.198604160177695 37.0 35.0 37.0 30.0 37.0 9 35.6868056211304 39.0 37.0 39.0 30.0 39.0 10-11 35.69686288382424 39.0 37.0 39.0 30.0 39.0 12-13 35.659098820780315 39.0 37.0 39.0 30.0 39.0 14-15 37.00459595482688 40.0 38.0 41.0 30.0 41.0 16-17 37.03997606890127 40.0 38.0 41.0 30.0 41.0 18-19 37.06103134861184 40.0 38.0 41.0 30.0 41.0 20-21 37.127284412229386 40.0 38.0 41.0 30.0 41.0 22-23 37.13826974491816 40.0 38.0 41.0 30.0 41.0 24-25 37.16427755000797 40.0 38.0 41.0 30.0 41.0 26-27 37.15375378591955 40.0 38.0 41.0 30.0 41.0 28-29 37.086030044944806 40.0 38.0 41.0 30.0 41.0 30-31 37.01105227775296 40.0 38.0 41.0 30.0 41.0 32-33 36.92826237391426 40.0 37.0 41.0 30.0 41.0 34-35 36.83843407743359 40.0 37.0 41.0 30.0 41.0 36-37 36.69713630156003 40.0 37.0 41.0 30.0 41.0 38-39 36.54671144704223 40.0 37.0 41.0 29.5 41.0 40-41 36.41938968651388 40.0 36.0 41.0 29.0 41.0 42-43 36.381680588891065 40.0 36.5 41.0 29.0 41.0 44-45 36.455870518380294 40.0 36.0 41.0 29.0 41.0 46-47 36.35881145028378 40.0 36.0 41.0 29.0 41.0 48-49 36.236196280109425 40.0 36.0 41.0 29.0 41.0 50-51 36.10301365538372 40.0 35.5 41.0 29.0 41.0 52-53 35.924617743683555 40.0 35.0 41.0 28.0 41.0 54-55 35.740340531924346 39.5 35.0 41.0 28.0 41.0 56-57 35.53506511993032 39.0 35.0 41.0 28.0 41.0 58-59 35.29043939921385 39.0 35.0 41.0 27.5 41.0 60-61 35.04055391050784 38.5 34.5 41.0 27.0 41.0 62-63 34.750177228248845 38.0 34.0 40.5 26.0 41.0 64-65 34.44632908502305 37.0 34.0 40.0 26.0 41.0 66-67 34.117513251599284 37.0 34.0 40.0 26.0 41.0 68-69 33.79419043686235 36.0 34.0 39.5 26.0 41.0 70-71 33.7018189326279 36.0 34.0 39.0 26.0 41.0 72-73 33.47813926546466 35.5 34.0 39.0 26.0 41.0 74-75 33.1445210045424 35.0 34.0 38.0 26.0 40.0 76-77 32.79922470568836 35.0 34.0 37.0 25.5 39.0 78-79 32.480658513932326 35.0 33.0 37.0 25.0 39.0 80-81 32.17293178509366 35.0 33.0 36.0 25.0 39.0 82-83 31.896682625430387 35.0 33.0 36.0 24.0 37.0 84-85 31.655328756639893 35.0 33.0 35.5 23.5 37.0 86-87 31.453619050169337 35.0 33.0 35.0 23.0 36.5 88-89 31.280640051188307 35.0 33.0 35.0 21.5 36.0 90-91 31.14662167018774 35.0 33.0 35.0 20.5 36.0 92-93 31.010165784166293 35.0 33.0 35.0 20.0 36.0 94-95 30.919653943346155 35.0 33.0 35.0 20.0 36.0 96-97 30.798316366870225 35.0 33.0 35.0 19.0 35.0 98-99 30.72073690307905 35.0 33.0 35.0 18.0 35.0 100-101 30.658363834444152 35.0 32.5 35.0 17.5 35.0 102-103 29.85784532730499 34.0 30.5 35.0 15.0 35.0 104-105 30.47441430115695 35.0 32.0 35.0 16.5 35.0 106-107 30.552174868963846 35.0 32.0 35.0 15.5 35.0 108-109 30.52297836476463 35.0 32.5 35.0 10.5 35.0 110-111 30.440341377546204 35.0 32.0 35.0 7.5 35.0 112-113 30.349131898688864 35.0 32.0 35.0 4.5 35.0 114-115 30.240713310229065 35.0 32.0 35.0 2.0 35.0 116-117 30.173342616382236 35.0 32.0 35.0 2.0 35.0 118-119 30.05215725184169 35.0 32.0 35.0 2.0 35.0 120-121 29.921921619309494 35.0 32.0 35.0 2.0 35.0 122-123 29.7053219211965 35.0 32.0 35.0 2.0 35.0 124-125 27.681262569816653 33.0 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36582437238729426 1101 2 0.3646890213155487 1101 3 0.3879408502742798 1101 4 0.42529807275833065 1101 5 0.513490540373617 1101 6 0.2924451487216899 1101 7 0.34841375650796635 1101 8 0.29185900853990887 1101 9 0.31274404765433417 1101 10-11 0.2107581697089458 1101 12-13 0.37189146040957155 1101 14-15 0.40634964626813286 1101 16-17 0.44997229528951266 1101 18-19 0.4685411660992784 1101 20-21 0.4412440698080218 1101 22-23 0.4444570838824973 1101 24-25 0.5070846615810751 1101 26-27 0.5549049884228694 1101 28-29 0.5535491865357471 1101 30-31 0.5133175216652432 1101 32-33 0.45090759205221786 1101 34-35 0.5145674140167174 1101 36-37 0.5395525779367745 1101 38-39 0.5068837670081763 1101 40-41 0.5254476816442946 1101 42-43 0.4524740484271632 1101 44-45 0.3620464331430284 1101 46-47 0.2641550681385283 1101 48-49 0.3114325585616342 1101 50-51 0.3021382490491433 1101 52-53 0.24286503713344132 1101 54-55 0.33020203277464333 1101 56-57 0.3895376448511314 1101 58-59 0.4134719282178594 1101 60-61 0.43358739343070596 1101 62-63 0.3588503247986665 1101 64-65 0.3696583993400253 1101 66-67 0.44057088752806806 1101 68-69 0.4411706281150245 1101 70-71 0.4163620784528206 1101 72-73 0.4307792921695892 1101 74-75 0.4476124642571122 1101 76-77 0.40093342055267556 1101 78-79 0.3597386843682173 1101 80-81 0.31790293389492774 1101 82-83 0.3649631985180193 1101 84-85 0.30035904213878695 1101 86-87 0.30206992949161915 1101 88-89 0.27112695708980183 1101 90-91 0.29429995015188126 1101 92-93 0.2961305693231999 1101 94-95 0.26445398778610496 1101 96-97 0.3303773650034252 1101 98-99 0.32541649750226753 1101 100-101 0.38150503449521267 1101 102-103 0.194833060573405 1101 104-105 0.1701774260853739 1101 106-107 0.21402045361642763 1101 108-109 0.19098228976741183 1101 110-111 0.1678375419381659 1101 112-113 0.16034268454315637 1101 114-115 0.32378702810072113 1101 116-117 0.29077996309776566 1101 118-119 0.25953259088881 1101 120-121 0.24805717679912576 1101 122-123 0.2059321676863739 1101 124-125 0.37123240950562675 1102 1 0.39618340500681626 1102 2 0.45918992395499103 1102 3 0.36338672303324415 1102 4 0.43189667833601675 1102 5 0.41859667832182623 1102 6 0.49702526575356387 1102 7 0.4184188922151364 1102 8 0.38697463976500757 1102 9 0.5347544435706695 1102 10-11 0.5705650359938019 1102 12-13 0.4768349676306869 1102 14-15 0.6859713936535883 1102 16-17 0.6500392151102261 1102 18-19 0.5661507278522677 1102 20-21 0.606639130191482 1102 22-23 0.4513474910040145 1102 24-25 0.39088734148645443 1102 26-27 0.2977344518192879 1102 28-29 0.3971065975168244 1102 30-31 0.44838973282062256 1102 32-33 0.35725680457711917 1102 34-35 0.28045503540615613 1102 36-37 0.3527353935493238 1102 38-39 0.20281683162470898 1102 40-41 0.3180818325407131 1102 42-43 0.14545213607656393 1102 44-45 0.16407464840697372 1102 46-47 0.38490838300406693 1102 48-49 0.35175953191820497 1102 50-51 0.15074577208201134 1102 52-53 0.31820235971810007 1102 54-55 0.44354942445791323 1102 56-57 0.43539328479137396 1102 58-59 0.0713988144230342 1102 60-61 0.3826388438789152 1102 62-63 0.4889763052518532 1102 64-65 0.3573113033489932 1102 66-67 0.33360422293642955 1102 68-69 0.2766782071737879 1102 70-71 0.35903863784027124 1102 72-73 0.2793187281755678 1102 74-75 0.4335301217521348 1102 76-77 0.5121915131821595 1102 78-79 0.3049441904688095 1102 80-81 0.39920273469174106 1102 82-83 0.2651515077809634 1102 84-85 0.1669104147732483 1102 86-87 0.37518761499958586 1102 88-89 0.34203084155195285 1102 90-91 0.34213789524650196 1102 92-93 0.29124386613594666 1102 94-95 0.3908176736675806 1102 96-97 0.20831872456517786 1102 98-99 0.3727272544743805 1102 100-101 0.24475321365357772 1102 102-103 0.17495748475169037 1102 104-105 0.24877514458636796 1102 106-107 0.23778477134550968 1102 108-109 0.05969299382617521 1102 110-111 0.09626640461246794 1102 112-113 0.21713310100231809 1102 114-115 0.20737766555091852 1102 116-117 0.2465992640190784 1102 118-119 0.2744008522184913 1102 120-121 0.28595247062382967 1102 122-123 0.06731087162063787 1102 124-125 -0.07795326965274185 1103 1 0.22457192187189534 1103 2 0.20154793486769051 1103 3 0.20755524981895945 1103 4 0.10898001166935245 1103 5 -0.028606942709920702 1103 6 0.2671455534519751 1103 7 -0.07744234290391461 1103 8 0.20166027964595656 1103 9 0.17888266777701745 1103 10-11 0.2106475012715805 1103 12-13 0.1961895063211685 1103 14-15 0.3447093028797781 1103 16-17 0.012545415507048574 1103 18-19 0.19710310880464732 1103 20-21 0.14488782810814627 1103 22-23 0.1222459285040145 1103 24-25 0.2727620314666126 1103 26-27 0.28866637146214913 1103 28-29 0.18089100971920402 1103 30-31 0.40746711377300215 1103 32-33 0.3853523526921947 1103 34-35 0.45410489899742856 1103 36-37 0.35804448333107075 1103 38-39 0.4138550880731202 1103 40-41 0.3068281123026182 1103 42-43 0.44455927893370273 1103 44-45 0.37906565783157475 1103 46-47 0.24275653528581387 1103 48-49 0.4108958166404264 1103 50-51 0.308383110871695 1103 52-53 0.3884296042617521 1103 54-55 0.5441043599737867 1103 56-57 0.4338741875691525 1103 58-59 0.5529991368436669 1103 60-61 0.40587483098209276 1103 62-63 0.38518941289073894 1103 64-65 0.28996724332915846 1103 66-67 0.44125861281737855 1103 68-69 0.3256070886221991 1103 70-71 0.23527096670932224 1103 72-73 0.0902841299612831 1103 74-75 0.22292589308150212 1103 76-77 0.0815739536583493 1103 78-79 0.3274748824330942 1103 80-81 0.22608765532666553 1103 82-83 0.2611522518285838 1103 84-85 0.32175757499150137 1103 86-87 0.11146923702339606 1103 88-89 0.3154931431392569 1103 90-91 0.4690987707425336 1103 92-93 0.43968136613594666 1103 94-95 0.35441359380646986 1103 96-97 0.11500275234295643 1103 98-99 0.16588201637914324 1103 100-101 0.19766895026072007 1103 102-103 0.2430765943747062 1103 104-105 0.3257298196855736 1103 106-107 0.44940431499630407 1103 108-109 0.43057748043331756 1103 110-111 0.47333578705294244 1103 112-113 0.40918264217295075 1103 114-115 0.42513560205885526 1103 116-117 0.36573213852304676 1103 118-119 0.32277169795261784 1103 120-121 0.28303828411589294 1103 122-123 0.20137895197778022 1103 124-125 0.4345327794544005 1104 1 0.2697251469384163 1104 2 0.39619882027001907 1104 3 0.31997955480106555 1104 4 0.38655708296029445 1104 5 0.4487591000462956 1104 6 0.3297602428730215 1104 7 0.2701526199367166 1104 8 0.18268491493360273 1104 9 0.30270136674022297 1104 10-11 0.3733803207963078 1104 12-13 0.3575231149091067 1104 14-15 0.42283860373306936 1104 16-17 0.6085720005148545 1104 18-19 0.5803607471200891 1104 20-21 0.5304249186669097 1104 22-23 0.6516293581764643 1104 24-25 0.6425121232181468 1104 26-27 0.5044401809859593 1104 28-29 0.5054420006358029 1104 30-31 0.586365407194414 1104 32-33 0.5213051924022665 1104 34-35 0.36833154823130343 1104 36-37 0.4738865330604014 1104 38-39 0.3808532071666164 1104 40-41 0.48413509000699406 1104 42-43 0.4957652381960216 1104 44-45 0.40016851130437203 1104 46-47 0.15544347904212685 1104 48-49 0.29500789114267434 1104 50-51 0.43653706995051067 1104 52-53 0.32181225404613656 1104 54-55 0.3872493732777542 1104 56-57 0.4147067180765376 1104 58-59 0.40356742292496506 1104 60-61 0.5169773403866245 1104 62-63 0.32626198835637865 1104 64-65 0.23401887650651076 1104 66-67 0.4370953868333487 1104 68-69 0.48221262582745084 1104 70-71 0.5371822659110848 1104 72-73 0.6084165274289433 1104 74-75 0.5130127129732287 1104 76-77 0.25134583639988506 1104 78-79 0.34438583756659824 1104 80-81 0.37481689654144645 1104 82-83 0.514575731046861 1104 84-85 0.4136467391350038 1104 86-87 0.3496935007173114 1104 88-89 0.5072825274864421 1104 90-91 0.4779958025803168 1104 92-93 0.37122038347698805 1104 94-95 0.390199560408913 1104 96-97 0.3779884620410918 1104 98-99 0.514104903800007 1104 100-101 0.4151200925673528 1104 102-103 0.26887024516835467 1104 104-105 0.30700103722846706 1104 106-107 0.3200117101701707 1104 108-109 0.3379973125280067 1104 110-111 0.48600610084320195 1104 112-113 0.5269715448245691 1104 114-115 0.5955640817358905 1104 116-117 0.5937089024463411 1104 118-119 0.5329744688490301 1104 120-121 0.48237136513249723 1104 122-123 0.2786566838797917 1104 124-125 0.3614612820693175 1105 1 0.03481249002385667 1105 2 0.05961040685421182 1105 3 -0.003991905557310105 1105 4 -0.06387376957050606 1105 5 -0.0802261217025162 1105 6 0.18009222351889775 1105 7 0.20721419254376627 1105 8 0.11362937595050226 1105 9 0.10669676647719228 1105 10-11 0.08148218697968446 1105 12-13 0.15707303207810952 1105 14-15 0.146473862160164 1105 16-17 0.13100813246660437 1105 18-19 0.1909073003936541 1105 20-21 0.21845363466446344 1105 22-23 0.18285936569972705 1105 24-25 0.14242160009769833 1105 26-27 0.22871801285872806 1105 28-29 0.208683760217653 1105 30-31 -0.005800262310827975 1105 32-33 0.07107977576139035 1105 34-35 0.19390944220386785 1105 36-37 -0.019377233489379364 1105 38-39 0.21519026836400457 1105 40-41 0.27447979079290263 1105 42-43 0.23144288583313966 1105 44-45 0.26883165835707246 1105 46-47 0.2595047308314733 1105 48-49 0.17035567815725017 1105 50-51 0.21226017614116444 1105 52-53 0.13825669348757685 1105 54-55 0.011582644698904687 1105 56-57 0.13111466685077033 1105 58-59 -0.006124273149993087 1105 60-61 -0.057768022296833976 1105 62-63 0.1328633282861773 1105 64-65 0.004380950227037772 1105 66-67 -0.3751681039257875 1105 68-69 -0.2133388898016193 1105 70-71 -0.11976653271108972 1105 72-73 -0.12168902346756738 1105 74-75 -0.08625644181285708 1105 76-77 0.05842371254924217 1105 78-79 0.004577052415008609 1105 80-81 -0.015871510683140855 1105 82-83 0.06548584018116088 1105 84-85 0.05440081079929371 1105 86-87 0.07571003314710367 1105 88-89 -0.08827283420267307 1105 90-91 -0.012775060419272677 1105 92-93 0.04542016847284813 1105 94-95 0.055379865579702425 1105 96-97 0.05244527641522723 1105 98-99 0.08511771211800223 1105 100-101 -4.462257025572569E-4 1105 102-103 -0.21035727836296303 1105 104-105 -0.2373623272673342 1105 106-107 -0.008073158303957939 1105 108-109 -0.06527990585128407 1105 110-111 -0.06556768816382075 1105 112-113 -0.038755276097241165 1105 114-115 -0.19467321078793276 1105 116-117 -0.03901769146412448 1105 118-119 0.0017758864504777705 1105 120-121 -0.012653146464778331 1105 122-123 0.10209532421314194 1105 124-125 0.3618740299760326 1106 1 -0.2015867929801658 1106 2 -0.22155135949486393 1106 3 -0.1463760768100535 1106 4 -0.12099025512588923 1106 5 0.07151110997563848 1106 6 -0.0804009788221336 1106 7 -0.19996553250761906 1106 8 -0.14096898517472312 1106 9 -0.15441239888352243 1106 10-11 -0.12896730640012066 1106 12-13 0.0055583358201758415 1106 14-15 -0.21031004092331784 1106 16-17 -0.1164615834673981 1106 18-19 -0.11815496163664108 1106 20-21 -0.03549026575234393 1106 22-23 0.10368276553390388 1106 24-25 -0.05969643006007885 1106 26-27 -0.019989262922756268 1106 28-29 -0.0773817666044394 1106 30-31 0.07828667110413079 1106 32-33 0.13988312675045478 1106 34-35 0.18435403417061735 1106 36-37 0.22718872572747983 1106 38-39 -0.09075508933117504 1106 40-41 0.00913587967185947 1106 42-43 0.008217324118994895 1106 44-45 -1.4558950888954314E-4 1106 46-47 0.026739254056380446 1106 48-49 -0.02992573055768588 1106 50-51 -0.09570912711640034 1106 52-53 -0.14754967816159592 1106 54-55 -0.08270852364960746 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2216 34-35 -1.6909729141575482 2216 36-37 -1.4317740583272496 2216 38-39 -1.6202163271484196 2216 40-41 -1.5527014543600473 2216 42-43 -1.5108241465138477 2216 44-45 -1.6343276164952556 2216 46-47 -1.4665847145306259 2216 48-49 -1.5818793912840619 2216 50-51 -1.5093542167478873 2216 52-53 -1.568871615917942 2216 54-55 -1.6734762733448605 2216 56-57 -1.7085745321766694 2216 58-59 -1.6896538361762055 2216 60-61 -1.8064964260432887 2216 62-63 -1.819300821666772 2216 64-65 -1.893547398159626 2216 66-67 -1.645794332835223 2216 68-69 -1.594739557886136 2216 70-71 -1.7278495527347921 2216 72-73 -1.7365962016499168 2216 74-75 -1.5665910245630457 2216 76-77 -1.6085605631424826 2216 78-79 -1.5897049205225713 2216 80-81 -1.5885758607666283 2216 82-83 -1.572624130885984 2216 84-85 -1.650671570482217 2216 86-87 -1.718581275612273 2216 88-89 -1.4701669086583067 2216 90-91 -1.4759935604147678 2216 92-93 -1.5854185077076188 2216 94-95 -1.5588918215752692 2216 96-97 -1.5372756562169947 2216 98-99 -1.4360406176871017 2216 100-101 -1.5105792775417548 2216 102-103 -1.6004877587565112 2216 104-105 -1.5880415901960134 2216 106-107 -1.6648940816822417 2216 108-109 -1.6784014033826153 2216 110-111 -1.8290479187422441 2216 112-113 -1.8739980852624 2216 114-115 -1.930421955979778 2216 116-117 -1.8249513923886163 2216 118-119 -2.0037498554402546 2216 120-121 -2.209201676768938 2216 122-123 -2.38744118499206 2216 124-125 -2.711977643470025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16437.0 3 2936.0 4 2090.0 5 1574.0 6 1601.0 7 1626.0 8 1749.0 9 1919.0 10 2241.0 11 2538.0 12 2814.0 13 2896.0 14 2916.0 15 2791.0 16 2533.0 17 2610.0 18 2183.0 19 2086.0 20 2072.0 21 2031.0 22 2453.0 23 3122.0 24 4594.0 25 6154.0 26 8566.0 27 7769.0 28 8137.0 29 10024.0 30 11218.0 31 12719.0 32 16503.0 33 21969.0 34 31631.0 35 51380.0 36 113359.0 37 230599.0 38 109148.0 39 549.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.425173807872525 18.608296541885867 12.848672970549927 23.117856679691684 2 15.248814247355401 20.349136321030635 39.91049593707935 24.491553494534614 3 18.835670983955698 26.597206659027194 28.385012537475003 26.18210981954211 4 12.2279114880563 16.649843231904 36.67583966796743 34.446405612072276 5 13.09395925847607 40.25234369493198 32.97737365193487 13.67632339465708 6 31.823122174433404 36.54730740331928 17.705967681227452 13.923602741019861 7 29.449523439019647 33.770427565831945 19.924364796788534 16.855684198359874 8 27.603007603571356 35.490990578336515 19.062343647600187 17.843658170491942 9 28.05688366166372 16.777832906710568 18.76952222009224 36.395761211533475 10-11 25.179924048902414 27.850967287753747 26.35407248826873 20.6150361750751 12-13 25.83605411351661 25.12351860478063 26.83428035041159 22.20614693129117 14-15 22.73743800318997 26.113475747150982 24.632600401882403 26.516485847776643 16-17 22.68745423884802 29.714957792274145 25.228021786360337 22.369566182517495 18-19 22.971764432275865 28.234862980150886 26.791476826255945 22.001895761317307 20-21 23.87476269067549 27.297725072126532 26.52071761494087 22.30679462225711 22-23 23.664529349876574 27.4042347482751 26.334026041017033 22.597209860831292 24-25 23.05790039705304 27.593635875440643 26.636099549386376 22.71236417811994 26-27 22.758128381173318 27.990354252887983 26.315526143512198 22.9359912224265 28-29 22.76995371336111 27.872900610577712 26.526937583510456 22.830208092550727 30-31 22.723304039355547 27.560087184289916 26.690287887148067 23.02632088920647 32-33 22.515383509554265 27.839924118355068 26.498465172523627 23.146227199567036 34-35 23.143786925540073 27.758070062688855 26.290914062147657 22.807228949623415 36-37 23.244013871848196 27.330867258308555 26.192098740723146 23.2330201291201 38-39 22.900430917540778 27.39663088019224 26.330506354536258 23.372431847730724 40-41 23.041635098229232 27.415522861421906 26.517557924411943 23.025284115936923 42-43 22.89354546812763 27.29835401967058 26.462326247452385 23.345774264749412 44-45 22.766712964822563 27.43859856821015 26.586627936885115 23.208060530082175 46-47 23.164351866534453 27.653140115003527 26.142758576459585 23.03974944200243 48-49 22.975142314723072 27.550636417065643 26.15652294018906 23.317698328022225 50-51 23.04496528744365 27.429297524417894 26.428549782619264 23.097187405519197 52-53 23.3061591521881 27.295393778972993 26.36698957148515 23.031457497353756 54-55 22.693100760662595 27.555027180615436 26.463221956468736 23.288650102253236 56-57 22.87649365888725 27.733444216179265 26.406867218966607 22.983194905966883 58-59 23.074570059204962 27.103185790809135 26.387158161826896 23.435085988159006 60-61 22.678177678399656 27.45800526691505 26.70158211813985 23.162234936545442 62-63 22.695646229642904 27.53652807621265 26.79035671473181 22.97746897941263 64-65 22.49105028751832 27.448204419889503 26.667676739203973 23.393068553388204 66-67 22.51309859800077 27.63830604385344 26.46188105380731 23.386714304338483 68-69 22.727279133517023 27.284418668564626 26.818761583290463 23.16954061462789 70-71 22.557935002297384 27.282234581438814 26.755668060132322 23.404162356131483 72-73 22.706593341545293 27.496004419802293 26.38781228242762 23.409589956224792 74-75 22.458588357170793 27.88302906655109 26.532133884431474 23.126248691846637 76-77 22.825789718506407 27.450981774100335 26.51551245365998 23.20771605373328 78-79 22.7825595505713 27.543504579837442 26.465526839862836 23.208409029728426 80-81 22.792766254925947 27.754584867260128 26.41140057617391 23.04124830164001 82-83 22.8014640698266 27.675401569209996 26.555997006464853 22.967137354498547 84-85 22.667826109016236 27.658267051800717 26.4701674966686 23.203739342514446 86-87 22.75216466308632 27.48359281572158 26.607157076987804 23.1570854442043 88-89 22.668326048870966 27.658452912680886 26.456129025892842 23.217092012555305 90-91 22.60398747562532 27.781955231632573 26.556579207931257 23.05747808481085 92-93 22.61780082572286 27.49940445901621 26.70920262348137 23.17359209177956 94-95 22.413066549031267 27.202245971668848 26.954055954798694 23.430631524501187 96-97 22.742884766621472 27.238441004941272 26.772078575515764 23.246595652921496 98-99 22.576330525303977 27.430293736689816 26.48753440740339 23.505841330602824 100-101 22.197573910874276 27.354669312523516 26.97413947405139 23.473617302550817 102-103 22.394917241690344 27.081430681459434 26.849870901546925 23.673781175303297 104-105 22.032872240561314 27.289160702153993 26.89513639682356 23.78283066046113 106-107 22.24041875194669 27.349454644366677 26.795642792412515 23.614483811274113 108-109 22.42349588534495 27.289415492074408 26.36423470516689 23.92285391741375 110-111 22.25787174908496 27.517825154926285 26.69467577878909 23.529627317199672 112-113 22.162563284359603 27.6104445972916 26.997685681103206 23.229306437245594 114-115 22.15682626662382 27.679049616362516 26.637788150929353 23.526335966084304 116-117 21.90882492220268 27.660612005592387 26.84754543814549 23.58301763405944 118-119 21.809872480170824 27.83935109003203 26.565844136166795 23.78493229363035 120-121 21.844109773036223 27.97129320799951 26.77583459768843 23.408762421275835 122-123 21.801783137148785 27.86616887656158 26.74769144905967 23.584356537229965 124-125 21.802517476204716 27.97116637798252 26.487593164109885 23.738722981702878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 747.0 1 762.0 2 803.5 3 683.5 4 436.0 5 301.0 6 259.0 7 239.5 8 258.0 9 267.5 10 231.0 11 215.5 12 206.0 13 220.0 14 239.5 15 254.0 16 267.5 17 295.5 18 348.0 19 400.5 20 497.5 21 669.5 22 916.5 23 1249.0 24 1647.5 25 2168.0 26 2890.5 27 3879.0 28 4855.0 29 6023.5 30 7475.0 31 8929.5 32 11021.5 33 12374.0 34 13269.0 35 14382.0 36 16024.0 37 17880.5 38 18783.5 39 19736.5 40 20685.5 41 21644.0 42 22290.5 43 23297.0 44 24770.0 45 26242.5 46 26435.0 47 26207.5 48 26438.5 49 25729.5 50 24993.5 51 24271.5 52 23743.5 53 23953.0 54 24690.5 55 23915.5 56 21638.5 57 20214.5 58 19501.5 59 17743.5 60 14704.5 61 12035.0 62 10163.0 63 8037.0 64 6175.0 65 4915.5 66 3586.0 67 2760.5 68 2039.5 69 1276.0 70 916.0 71 696.5 72 533.0 73 423.5 74 341.5 75 272.5 76 175.5 77 97.0 78 60.0 79 38.5 80 37.0 81 28.0 82 14.5 83 9.5 84 6.5 85 6.5 86 7.5 87 5.5 88 4.5 89 6.0 90 5.0 91 3.0 92 3.0 93 3.0 94 2.0 95 1.5 96 1.5 97 0.5 98 0.5 99 3.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01874461796918272 2 0.010147462359256811 3 0.00901996654156161 4 0.029455828237287134 5 0.02635521473862533 6 0.023395538217175424 7 0.011134021199740112 8 2.8187395442380033E-4 9 0.012543390971859114 10-11 2.1140546581785023E-4 12-13 0.004016703850539155 14-15 0.01768759064009347 16-17 0.0014798382607249516 18-19 0.008385750144108058 20-21 0.002818739544238003 22-23 3.5234244302975037E-4 24-25 0.00845621863271401 26-27 2.1140546581785023E-4 28-29 0.006342163974535507 30-31 7.751533746654509E-4 32-33 0.0015503067493309018 34-35 1.4093697721190017E-4 36-37 0.005989821531505756 38-39 6.342163974535507E-4 40-41 0.014023229232584065 42-43 0.007187785837806908 44-45 7.751533746654509E-4 46-47 0.011345426665557963 48-49 0.002536865589814203 50-51 0.00951324596180326 52-53 0.005637479088476006 54-55 0.0033824874530856038 56-57 0.011204489688346062 58-59 0.01930836587803032 60-61 0.0019026491923606521 62-63 0.006835443394777157 64-65 0.0023959286126023025 66-67 0.002043586169572552 68-69 2.8187395442380033E-4 70-71 0.004932794202416505 72-73 4.2281093163570047E-4 74-75 0.006271695485929557 76-77 0.015080256561673317 78-79 0.001973117680966602 80-81 0.004087172339145104 82-83 0.0011274958176952013 84-85 7.046848860595008E-5 86-87 0.0021845231467844525 88-89 0.005073731179628406 90-91 0.0057079475770819565 92-93 0.013811823766766214 94-95 0.0 96-97 4.2281093163570047E-4 98-99 0.0033824874530856038 100-101 0.0 102-103 1.4093697721190017E-4 104-105 0.00887902956434971 106-107 0.0 108-109 0.0 110-111 8.456218632714009E-4 112-113 0.005778416065687906 114-115 0.001832180703754702 116-117 1.4093697721190017E-4 118-119 0.005567010599870056 120-121 0.002607334078420153 122-123 1.4093697721190017E-4 124-125 0.0807568879424188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 709537.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.73128036507856 #Duplication Level Percentage of deduplicated Percentage of total 1 77.27683959871958 43.84014053002166 2 12.309967569778916 13.967204429723049 3 4.249389624057463 7.232199424285793 4 1.9553699612853512 4.437225659645283 5 1.1333555851720478 3.2148356727861565 6 0.645540266671336 2.1973395513287493 7 0.444713980371743 1.7660435451917575 8 0.32046631240855866 1.4544371373450236 9 0.23564115429810562 1.203140195103291 >10 1.321247403559199 13.659725727483178 >50 0.07438230012723246 2.8528094551247776 >100 0.030847324814134765 3.3109026720734245 >500 0.001990149987818921 0.7172211158820256 >1k 2.487687484773651E-4 0.14677488400576674 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1040 0.14657445630037616 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 854 0.12036017853896273 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 717 0.10105181266093241 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.046848860595008E-5 0.0 20-21 0.0 0.0 0.0 1.4093697721190017E-4 0.0 22-23 0.0 0.0 0.0 2.8187395442380033E-4 0.0 24-25 0.0 0.0 0.0 5.637479088476007E-4 0.0 26-27 0.0 0.0 0.0 7.751533746654508E-4 0.0 28-29 7.046848860595008E-5 0.0 0.0 0.0018321807037547022 0.0 30-31 1.4093697721190017E-4 0.0 0.0 0.004791857225204605 0.0 32-33 1.4093697721190017E-4 0.0 0.0 0.011063552711134163 0.0 34-35 1.4093697721190017E-4 0.0 0.0 0.01888555494639462 0.0 36-37 1.4093697721190017E-4 0.0 0.0 0.03565705523461074 0.0 38-39 1.4093697721190017E-4 0.0 0.0 0.06158945904160036 0.0 40-41 1.4093697721190017E-4 0.0 0.0 0.09745791974202896 0.0 42-43 1.4093697721190017E-4 0.0 0.0 0.1302962354324017 0.0 44-45 1.4093697721190017E-4 0.0 0.0 0.16679891253028384 0.0 46-47 1.4093697721190017E-4 0.0 0.0 0.2110531233748205 0.0 48-49 1.4093697721190017E-4 0.0 0.0 0.26038106539898553 0.0 50-51 1.4093697721190017E-4 0.0 0.0 0.3227456778152513 0.0 52-53 1.4093697721190017E-4 0.0 0.0 0.37905000021140545 0.0 54-55 1.4093697721190017E-4 0.0 0.0 0.4383139991290095 0.0 56-57 2.8187395442380033E-4 0.0 0.0 0.5104737314615023 0.0 58-59 2.8187395442380033E-4 0.0 0.0 0.5963748190721555 0.0 60-61 2.8187395442380033E-4 0.0 0.0 0.6928461799737011 0.0 62-63 2.8187395442380033E-4 0.0 0.0 0.7955892363611764 0.0 64-65 2.8187395442380033E-4 0.0 0.0 0.8886781098096364 0.0 66-67 2.8187395442380033E-4 0.0 0.0 0.9945922481843794 0.0 68-69 2.8187395442380033E-4 0.0 0.0 1.1182644456878217 0.0 70-71 2.8187395442380033E-4 0.0 0.0 1.2608221981376588 0.0 72-73 3.523424430297504E-4 0.0 0.0 1.414302566321418 0.0 74-75 4.2281093163570047E-4 0.0 0.0 1.5592562473838574 0.0 76-77 4.2281093163570047E-4 0.0 0.0 1.7123842731245869 0.0 78-79 4.2281093163570047E-4 0.0 0.0 1.9064544907453733 0.0 80-81 5.637479088476007E-4 0.0 0.0 2.1334334925451386 0.0 82-83 5.637479088476007E-4 0.0 0.0 2.372674011362339 0.0 84-85 5.637479088476007E-4 0.0 0.0 2.6006395720025877 0.0 86-87 5.637479088476007E-4 0.0 0.0 2.836920414298338 0.0 88-89 5.637479088476007E-4 0.0 0.0 3.106532851704703 0.0 90-91 5.637479088476007E-4 0.0 0.0 3.420469968444211 0.0 92-93 5.637479088476007E-4 0.0 0.0 3.7639333819096112 0.0 94-95 5.637479088476007E-4 0.0 0.0 4.089004519848859 0.0 96-97 5.637479088476007E-4 0.0 0.0 4.412032071618535 0.0 98-99 7.046848860595007E-4 0.0 0.0 4.760780621729381 0.0 100-101 7.046848860595007E-4 0.0 0.0 5.181829841149932 0.0 102-103 7.046848860595007E-4 0.0 0.0 5.639522674645579 0.0 104-105 7.046848860595007E-4 0.0 0.0 6.095242390460258 0.0 106-107 7.046848860595007E-4 0.0 0.0 6.5401804275182265 0.0 108-109 7.046848860595007E-4 0.0 0.0 6.998225603456902 0.0 110-111 7.046848860595007E-4 0.0 0.0 7.5357592345430895 0.0 112-113 7.046848860595007E-4 0.0 0.0 8.12177518579017 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1030 0.0 65.30586 1 GTATCAA 1765 0.0 54.636295 1 ATCAACG 2110 0.0 43.728054 3 GTACATG 5110 0.0 43.45091 1 TATCGTC 55 8.52817E-4 43.270702 7 TCAACGC 2170 0.0 42.51899 4 TACATGG 5260 0.0 41.532806 2 CAACGCA 2235 0.0 41.282417 5 TATCAAC 2390 0.0 39.352303 2 AACGCAG 2360 0.0 39.334213 6 ACATGGG 5425 0.0 38.184795 3 TAAGGTG 415 0.0 37.293762 5 GTAAGGT 370 0.0 37.003014 4 CATGGGG 1995 0.0 34.01519 4 CATGGGA 3525 0.0 33.09847 4 ATGGGAG 1445 0.0 32.131996 5 ACGCAGA 2885 0.0 31.965576 7 AATAACG 75 0.0039103827 31.731846 7 CGCAGAG 3000 0.0 30.742395 8 GGTGCGT 90 0.009528724 26.443207 7 >>END_MODULE