FastQCFastQC Report
Fri 27 May 2016
SRR940130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709537
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38780.5465535976277488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31120.4385958730834333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20610.2904711100337262No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.12021924156175082No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA7630.10753491361267982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.082.52421
GTATCAA38750.062.446271
GCGTATC350.007743031751.086411
ATCAACG50250.048.0229153
TCAACGC50850.047.4529234
TATCAAC51500.046.9730072
CAACGCA52450.045.9534725
AACGCAG53950.044.6726576
GTGGTAT7450.042.4005781
TGGTATC7350.042.1546552
GTACATG48700.039.8970571
ACGCAGA61700.038.9567387
CGCAGAG61900.038.6386348
TACATGG50000.038.252922
ACATGGG50800.036.594893
GCAGAGT68000.034.6475569
CATGGGA33000.032.1369744
CATGGGG18950.031.1261124
ATGGGAT13250.030.5423075
GAGTACT42800.029.95641712-13
Produced by FastQC (version 0.11.5)